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The map label for this gene is ansA [H]

Identifier: 218902707

GI number: 218902707

Start: 1501961

End: 1502893

Strand: Reverse

Name: ansA [H]

Synonym: BCAH820_1590

Alternate gene names: 218902707

Gene position: 1502893-1501961 (Counterclockwise)

Preceding gene: 218902711

Following gene: 218902694

Centisome position: 28.34

GC content: 36.44

Gene sequence:

>933_bases
ATGGAGGAAGATAAAGAAACTGGGGTCGTACAACCAGGTGAAAAAAATCCTCTTTTAAAATTCATTCCTGATTTAGAAGG
CGATGTTGATTTAATCGTTGAAGATGTGTTCCATCTACCATCTCCTCATATGACACCAAGCGAAATGTTACAATTACAAG
TCATCATTGATGAAAGAGTAAAACAAGACAACATTCATGGCGTAGTCATTACGCATGGTACGGATACATTAGAAGAAACA
GCCTACTTCTTAGATTTAACAGTACAAGCAACTATTCCGATCGTTGTAACAGGTGCAATGCGATCTAGTAATGAATTAGG
TGCTGATGGTCTATATAACTTCTTATCAGCTGTAAAAGTCGCTAGCAGTAGTGAAGCCGCAGAAAAAGGTGTTTTAGTCG
TATTAAACGACGAAATTCATTGCGCTACAAACGTAACAAAAACACATACAAGCAATGTAGCAACATTCCAAAGTCCACAG
TATGGACCAATTGGTATGGTAACAAAGCGCGGCGTTGTATTCCACCATGCTTTAGTACATCATGAAACATATTCAGTAAA
TAAAATTTCTAAAAATGTAGTCGTTCTAAAAGCTTACGCTGGTATGGACGATACATTGTTAGCAGCAATTGAAAATCTTC
CAGTAGACGGGATTGTTATTGAAGCACTTGGACAAGGTAATTTACCTCCAAGAACACTTCCTAGTCTAGAACGATTAATA
AACAAAGGAATTCCTGTCGTATTAGTTTCTCGCTGTTTCAACGGCATCGTTCAAGATGTATATTCATATGAAGGTGGCGG
CAAACAATTAAAAGATATGGGTATTGTATTCACATACGGTTTAAACGCTCAAAAAGCACGTATTAAATTATTAGTTGCAT
TAGAGAATACAACTTCTCATGAAACAATCCAAAACATGTTTATGCATGATTAA

Upstream 100 bases:

>100_bases
ATTTACTCAAAACATGTTATGTTCTAAATGTATTTTTTACTTGAAAGGGGTATAAGCATTGAAAAGAATCCTAGTTTTAC
ACACAGGTGGAACAATTGCA

Downstream 100 bases:

>100_bases
AAAAAGAAGCTGTCGAAGTTTATTTCGACAGCTTCTTTTTACATTTCTCTTGTTGCGATGGTTTGCGCAATTGCATCTCC
ATGAAATCGACCGTTCTCAA

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYSVNKISKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHETIQNMFMHD

Sequences:

>Translated_310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYSVNKISKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHETIQNMFMHD
>Mature_310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYSVNKISKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHETIQNMFMHD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=235, Percent_Identity=28.5106382978723, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789327, Length=299, Percent_Identity=36.1204013377926, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1788065, Length=272, Percent_Identity=27.2058823529412, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=237, Percent_Identity=27.0042194092827, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17558278, Length=237, Percent_Identity=27.0042194092827, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=275, Percent_Identity=26.5454545454545, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=237, Percent_Identity=27.0042194092827, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323189, Length=304, Percent_Identity=29.9342105263158, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323187, Length=304, Percent_Identity=29.9342105263158, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323186, Length=304, Percent_Identity=29.9342105263158, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323184, Length=304, Percent_Identity=29.9342105263158, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320527, Length=299, Percent_Identity=29.0969899665552, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24645530, Length=238, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639609, Length=233, Percent_Identity=24.0343347639485, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33836; Mature: 33836

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERV
CCCCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHEEEEEEEHHHC
KQDNIHGVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKV
CCCCCCEEEEECCCCCHHHHEEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
ASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVH
CCCCCHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHH
HETYSVNKISKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
CCCCCHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHH
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSH
HCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCEEEEEEEEECCCCCH
ETIQNMFMHD
HHHHHHHCCC
>Mature Secondary Structure
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERV
CCCCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHEEEEEEEHHHC
KQDNIHGVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKV
CCCCCCEEEEECCCCCHHHHEEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
ASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVH
CCCCCHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHH
HETYSVNKISKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
CCCCCHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHH
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSH
HCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCEEEEEEEEECCCCCH
ETIQNMFMHD
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]