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The map label for this gene is phaC [H]

Identifier: 218902523

GI number: 218902523

Start: 1339881

End: 1340966

Strand: Direct

Name: phaC [H]

Synonym: BCAH820_1406

Alternate gene names: 218902523

Gene position: 1339881-1340966 (Clockwise)

Preceding gene: 218902522

Following gene: 218902524

Centisome position: 25.27

GC content: 37.94

Gene sequence:

>1086_bases
ATGACTACATTCGCAACAGAATGGGAAAAGCAATTAGAGCTATACCCAGAAGAGTATCGAAAAGCATACCGCCGAGTGAA
AAGGGCGAGTGAAATTTTATTACGTGAACCAGAGCCGCAAGTCGGATTAACGCCGAAAGAGGTTATTTGGACGAAGAATA
AGACGAAGCTTTATCGCTACATTCCAAAACAAGAAAAAACACAAAGAGTTCCAATTCTATTAATATATGCTCTTATTAAT
AAACCATATATTATGGACTTAACTCCTGGAAATAGTTTAGTGGAATATCTAGTGGACCGTGGTTTTGATGTGTATATGCT
TGATTGGGGCACATTTGGTTTAGAAGATAGTCATTTGAAATTTGATGATTTCGTGTTTGATTATATTGCAAAAGCAGTGA
AAAAAGTAATGCGAACTGCAAAATCGGACGAGATTTCTTTACTTGGTTATTGCATGGGTGGAACGTTAACTTCTATTTAT
GCGGCACTTCATCCGCACATGCCAATTCGCAACTTAATCTTTATGACAAGTCCTTTTGATTTCTCTGAAACAGGATTATA
TGGTCCTTTACTAGATGAAAAATATTTTAACTTAGATAAAGCAGTTGATACATTTGGCAATATTCCGCCAGAAATGATTG
ATTTCGGCAACAAAATGTTAAAACCAATTACAAACTTTGTTGGTCCATACGTTGCTTTAGTAGATCGTTCAGAGAATGAG
CGTTTCGTTGAAAGCTGGAGGTTAGTTCAAAAGTGGGTTGGCGATGGCATTCCGTTCCCGGGTGAATCATACAGACAGTG
GATTCGTGATTTTTATCAAAATAATAAACTCGTTAAGGGTGAACTCGTTATTCGCGGACAAAAGGTAGACCTTGCAAATA
TTAAGGCGAATGTCTTAAATATTTCCGGGAAACGTGATCATATCGCTCTGCCATGCCAAGTAGAAGCATTGCTAGATCAT
ATTTCTAGCACAGATAAACAATATGTATGTTTACCGACAGGGCATATGTCTATCGTTTACGGCGGAACAGCTGTAAAACA
AACGTATCCGACGATTGGAGATTGGCTTGACGAGCGTTCAAAGTAA

Upstream 100 bases:

>100_bases
GGCGGATTATATATGTAATGAAGTAAGAAAAAAGTGCATATCCATAGCAGGAATGCACTTCTTTTTTTAGAAGGAAATCG
ACCAAAAAGGAGATAGAAAA

Downstream 100 bases:

>100_bases
AAATGAAAAATCCAACTAGCGTTGTGTTAGTTGGATTTTTTTATGGAAAATAACAAAACATAGAAAGGAGAAATTTATAT
TGTAGGAGTATAATTATGAA

Product: poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRYIPKQEKTQRVPILLIYALIN
KPYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLKFDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIY
AALHPHMPIRNLIFMTSPFDFSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENE
RFVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLNISGKRDHIALPCQVEALLDH
ISSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGDWLDERSK

Sequences:

>Translated_361_residues
MTTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRYIPKQEKTQRVPILLIYALIN
KPYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLKFDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIY
AALHPHMPIRNLIFMTSPFDFSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENE
RFVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLNISGKRDHIALPCQVEALLDH
ISSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGDWLDERSK
>Mature_360_residues
TTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRYIPKQEKTQRVPILLIYALINK
PYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLKFDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIYA
ALHPHMPIRNLIFMTSPFDFSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENER
FVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLNISGKRDHIALPCQVEALLDHI
SSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGDWLDERSK

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010125 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 41682; Mature: 41550

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRY
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCHHHHHC
IPKQEKTQRVPILLIYALINKPYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLK
CCCCCCCCCCHHHEEEHHHCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
FDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIYAALHPHMPIRNLIFMTSPFD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCHHHEEEEECCCC
FSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCH
RFVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCEEHHHCEEEEEE
ISGKRDHIALPCQVEALLDHISSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGDWLDERS
ECCCCCCEECCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCHHHHCCCCHHHHHHHCC
K
C
>Mature Secondary Structure 
TTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRY
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCHHHHHC
IPKQEKTQRVPILLIYALINKPYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLK
CCCCCCCCCCHHHEEEHHHCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
FDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIYAALHPHMPIRNLIFMTSPFD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCHHHEEEEECCCC
FSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCH
RFVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCEEHHHCEEEEEE
ISGKRDHIALPCQVEALLDHISSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGDWLDERS
ECCCCCCEECCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCHHHHCCCCHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1396692 [H]