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The map label for this gene is nrgA [H]

Identifier: 218902355

GI number: 218902355

Start: 1182396

End: 1183628

Strand: Reverse

Name: nrgA [H]

Synonym: BCAH820_1237

Alternate gene names: 218902355

Gene position: 1183628-1182396 (Counterclockwise)

Preceding gene: 218902358

Following gene: 218902351

Centisome position: 22.32

GC content: 43.8

Gene sequence:

>1233_bases
ATGAATATAGGAGATACGGTTTTTATGTTTGTAGCGACAGTAATGGTCATGTTAATGACACCAGGATTGGCACTTTTTTA
TGGAGGGATGGTTCGCAGTAAAAACGTACTCAGTACGACAATGCATAGTTATAGCGCGATGGCTATCGTTTCGATTCAAT
GGATTGTAATTGGTTATTCTTTATCATTCGGACCAGATTGGCACGGACTTATCGGCACACTCGATTGGTTCGGTTTAAAT
GGAGTTACCTACGCACCAAATCCAGACTATTCATCTACTATTCCTCACAATTTATTTATGATGTTTCAACTCATGTTCGC
TATTTTAACTCCTGCTTTAATTTCAGGTGCATTTGCCGAAAGAATGCGATTTTCAGCTTTCCTTATTTTCATCCTTCTAT
GGACAACGATCGTTTACAATCCTGTTGCTCATTGGGTATGGGGCGTTGGCGGATGGCTAAGAGAACTTGGCGCGTTAGAC
TTCGCTGGCGGTAATGTCGTTCATATTACATCTGGTGTGGCTGGCCTCGTATTAGCTATTTTCCTTGGAAAACGAAAAAA
CATAAACGGTACTTCTCCTCATCATTTACCATTTACGATGCTAGGCGCTGGGCTGCTATGGTTCGGTTGGTTCGGTTTTA
ACGTCGGAAGCGCATTATCTTTAAATGATGTAGCTTTAACTGCATTTATTAATACGAATATAGCCGCTGCTGCCTCCGCA
TTAACTTGGATGCTTTCAGAGTGGTTCTTTCAATCAAAACCAACTGCGATGGGAGCTGCTTGTGGCGCCGTTTCCGGTCT
AGTCGCTATTACACCAGCTTGCGGGTTCGTTACACCTTTCTCCGCTCTTCTTATCGGAGCGATTGGCGGCGTATTATGCT
TCGGAGCAGTCTTTTTCTTAAAAACAAAATTCGGATACGACGATACACTCGATGCATTCGGATGTCACGGCATCGGCGGA
ACTTGGGGCGGTATTGCGACAGGACTATTTGCAACTACTACCGTAAACGGCGATGGCGCTAACGGATTATTTTACGGAAA
CGCCGCGTTGCTGTTTAAACAACTTGTAGCAATCGGCGCAACATATGCATTCACAATTATAATGACGTACGCCATTATTA
AAGCAATCAACTTCTTCCTCCCTGTCCGGGTCGATGAGCACGAGGAACATATGGGACTTGATATTTCGATGCATGGAGAG
AAAGCTCACGAGTATACTGAGCGAGTGAACTAA

Upstream 100 bases:

>100_bases
ATTTTTCTGAATTTTTAGTTGATTTTTCTTTTCACTTCGAATATGATATGAAACATAACAAATTGATGTTATTTTTTCTA
ACATTACAGGAGGAGAGAAA

Downstream 100 bases:

>100_bases
AAAAAGAGATGCAATCTATTGATAGTAGATTGCATCTCTTTTTTTGCATTTGTCGGCACAGCATACCCTAAGCGGTATTC
CTGTAAATATATCAGCGATT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNIGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAHEYTERVN

Sequences:

>Translated_410_residues
MNIGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAHEYTERVN
>Mature_410_residues
MNIGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAHEYTERVN

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=404, Percent_Identity=42.8217821782178, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=29.1375291375291, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=28.2660332541568, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17550182, Length=442, Percent_Identity=27.6018099547511, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=27.4599542334096, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324187, Length=414, Percent_Identity=40.0966183574879, Blast_Score=256, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6325396, Length=412, Percent_Identity=36.4077669902913, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=36.3636363636364, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=30.2884615384615, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44070; Mature: 44070

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEE
LSFGPDWHGLIGTLDWFGLNGVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHH
FLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHEEEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAHEYTERVN
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHCCCEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MNIGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEE
LSFGPDWHGLIGTLDWFGLNGVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHH
FLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHEEEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAHEYTERVN
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHCCCEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]