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The map label for this gene is cisA [H]

Identifier: 218901967

GI number: 218901967

Start: 811041

End: 812495

Strand: Reverse

Name: cisA [H]

Synonym: BCAH820_0849

Alternate gene names: 218901967

Gene position: 812495-811041 (Counterclockwise)

Preceding gene: 218901969

Following gene: 218901966

Centisome position: 15.32

GC content: 36.56

Gene sequence:

>1455_bases
ATGAACGAACAGTCTTCTCAATTTATTGCGTTATACTGCCGAGTTTCCACAGATGACCAAGCAAAAGAAGGCGTTTCATT
AGAAGAACAACAAGAACGTTTAGAAGCCTATTGCCGCGCGATGGGTTGGGGTGAAGATGTCGTCGTTTATGTTGAAGATG
GCTACTCAGCTAAAAGCACCGACCGCCCACATTTAAAGAGACTTATTGAAGATGTAAAAGCTGGTGAAATCAAAAAAATC
ATTGTAACAAAACTAGATCGAATGAGTAGAAAATTATTAGACTTGCTCACATTAATTGATTTATTTCAAGAACACGATGT
TTCATTCGTTTCTATTAGTGAGTCATTCGATACAAATACACCATCGGGGCGGTTAACTCTTCAAGTATTAGGAGCAGTCG
CCGAATTTGAAAGAGAACGCATTCGTGAACGTGTCTTTGAAAATATGTATCATGCTGCTTCTAAAGGAAAATGGCTTACA
CAACACCCTTTTGGTTATCGATTAGAAAACAAAGAACTCGTTATTTATGAAAATGAAGCAAAAATTGTTCGAGATATATT
CATTTGGTACACACAAGAAGGTCACGGTTTCTATGCGATTGCAAAAAAACTAAATTTACTAGGCATCCCTTCTCGGCAAA
ATAAACAGTGGTCTATTCGTTCCGTCAAATTACTACTATCTAATCCTGTATACAAAGGGACTGTAGTTTGGAATCGAAAA
GATCGATCAAAGAAAAAAGAACAATTCAAAAAAGAAGACGAGTGGGTTATCCAAGAGAATGCTGCTCCAGCCATTGTTAC
AGATGAATTATGGAATGAAGCTCAAAAACGGATGCAAACTTCTACCCTTGCTCCACGCGCTCAAACAAGCCCACACCTTC
TAGGAGGATTACTGAAGTGTGGAAAGTGCGGATCTGGAATGAGCATTGGTTGGTCAGGCTCAACGAAAAATCGTTACAGA
GTGTACAGATGCTCAGCTAATAAAAATAAAGGAACATGTACGAGCAAGCAATATAAAGCTCATCAGTTAGAGGAACTTTT
CAAAAAAGGGCTATCCAATCTATGTGAATCGTTTAGCTTTTCATTACATCCTTCATTATCTTTACAGAAAAGACAAACAG
ACGTAACAAATCAGATGAAACTGGCAACCGCCAAAAAGAGGTATAAACGAAAAGTTGAAGCTTTTACTGCAGGGTTAATC
GAGTTAGAGGACTTACAAGAAGAAAAAAGAAGACTAGAAACTTTAGAACTCGCATTACAACCTACTACTGAATTAAATGA
AAACATCAATTTACCTCAAATAGAAGAATGGATGAAAAGTAAATTAAAAAACATCTATGATGCATTATCAATCGTTCCTG
TTGAAGAGTTAAAACCGCTCATACAAACGATTATTGAAAAAGTGATTCTACATAGCGAAGAAGAACTAGAAATTGTTTTA
CAACCTATTATATAA

Upstream 100 bases:

>100_bases
AACATACCTATGCGGGGAGAAGATGAGGACAACCCTATAACTTGTTCTATCATGTTGAACTTTTTCTTTCATATACATAT
GGACAAGGAGGTGTTTTTTT

Downstream 100 bases:

>100_bases
ATATGGAGGCTTCATCATGAATACACATCATTTATCATCAACAAGCGGCAAATGGATTCCACATATTCAAATGCCGCATA
CTCCTCCTAATAAGGAAGCT

Product: cassette chromosome recombinase B

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNEQSSQFIALYCRVSTDDQAKEGVSLEEQQERLEAYCRAMGWGEDVVVYVEDGYSAKSTDRPHLKRLIEDVKAGEIKKI
IVTKLDRMSRKLLDLLTLIDLFQEHDVSFVSISESFDTNTPSGRLTLQVLGAVAEFERERIRERVFENMYHAASKGKWLT
QHPFGYRLENKELVIYENEAKIVRDIFIWYTQEGHGFYAIAKKLNLLGIPSRQNKQWSIRSVKLLLSNPVYKGTVVWNRK
DRSKKKEQFKKEDEWVIQENAAPAIVTDELWNEAQKRMQTSTLAPRAQTSPHLLGGLLKCGKCGSGMSIGWSGSTKNRYR
VYRCSANKNKGTCTSKQYKAHQLEELFKKGLSNLCESFSFSLHPSLSLQKRQTDVTNQMKLATAKKRYKRKVEAFTAGLI
ELEDLQEEKRRLETLELALQPTTELNENINLPQIEEWMKSKLKNIYDALSIVPVEELKPLIQTIIEKVILHSEEELEIVL
QPII

Sequences:

>Translated_484_residues
MNEQSSQFIALYCRVSTDDQAKEGVSLEEQQERLEAYCRAMGWGEDVVVYVEDGYSAKSTDRPHLKRLIEDVKAGEIKKI
IVTKLDRMSRKLLDLLTLIDLFQEHDVSFVSISESFDTNTPSGRLTLQVLGAVAEFERERIRERVFENMYHAASKGKWLT
QHPFGYRLENKELVIYENEAKIVRDIFIWYTQEGHGFYAIAKKLNLLGIPSRQNKQWSIRSVKLLLSNPVYKGTVVWNRK
DRSKKKEQFKKEDEWVIQENAAPAIVTDELWNEAQKRMQTSTLAPRAQTSPHLLGGLLKCGKCGSGMSIGWSGSTKNRYR
VYRCSANKNKGTCTSKQYKAHQLEELFKKGLSNLCESFSFSLHPSLSLQKRQTDVTNQMKLATAKKRYKRKVEAFTAGLI
ELEDLQEEKRRLETLELALQPTTELNENINLPQIEEWMKSKLKNIYDALSIVPVEELKPLIQTIIEKVILHSEEELEIVL
QPII
>Mature_484_residues
MNEQSSQFIALYCRVSTDDQAKEGVSLEEQQERLEAYCRAMGWGEDVVVYVEDGYSAKSTDRPHLKRLIEDVKAGEIKKI
IVTKLDRMSRKLLDLLTLIDLFQEHDVSFVSISESFDTNTPSGRLTLQVLGAVAEFERERIRERVFENMYHAASKGKWLT
QHPFGYRLENKELVIYENEAKIVRDIFIWYTQEGHGFYAIAKKLNLLGIPSRQNKQWSIRSVKLLLSNPVYKGTVVWNRK
DRSKKKEQFKKEDEWVIQENAAPAIVTDELWNEAQKRMQTSTLAPRAQTSPHLLGGLLKCGKCGSGMSIGWSGSTKNRYR
VYRCSANKNKGTCTSKQYKAHQLEELFKKGLSNLCESFSFSLHPSLSLQKRQTDVTNQMKLATAKKRYKRKVEAFTAGLI
ELEDLQEEKRRLETLELALQPTTELNENINLPQIEEWMKSKLKNIYDALSIVPVEELKPLIQTIIEKVILHSEEELEIVL
QPII

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=153, Percent_Identity=34.640522875817, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787827, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787638, Length=156, Percent_Identity=29.4871794871795, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 55809; Mature: 55809

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQSSQFIALYCRVSTDDQAKEGVSLEEQQERLEAYCRAMGWGEDVVVYVEDGYSAKST
CCCCCCCEEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
DRPHLKRLIEDVKAGEIKKIIVTKLDRMSRKLLDLLTLIDLFQEHDVSFVSISESFDTNT
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PSGRLTLQVLGAVAEFERERIRERVFENMYHAASKGKWLTQHPFGYRLENKELVIYENEA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCEEEEEECCH
KIVRDIFIWYTQEGHGFYAIAKKLNLLGIPSRQNKQWSIRSVKLLLSNPVYKGTVVWNRK
HHEEEEEEEEEECCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCC
DRSKKKEQFKKEDEWVIQENAAPAIVTDELWNEAQKRMQTSTLAPRAQTSPHLLGGLLKC
HHHHHHHHHCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GKCGSGMSIGWSGSTKNRYRVYRCSANKNKGTCTSKQYKAHQLEELFKKGLSNLCESFSF
CCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SLHPSLSLQKRQTDVTNQMKLATAKKRYKRKVEAFTAGLIELEDLQEEKRRLETLELALQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTTELNENINLPQIEEWMKSKLKNIYDALSIVPVEELKPLIQTIIEKVILHSEEELEIVL
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHE
QPII
ECCC
>Mature Secondary Structure
MNEQSSQFIALYCRVSTDDQAKEGVSLEEQQERLEAYCRAMGWGEDVVVYVEDGYSAKST
CCCCCCCEEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
DRPHLKRLIEDVKAGEIKKIIVTKLDRMSRKLLDLLTLIDLFQEHDVSFVSISESFDTNT
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PSGRLTLQVLGAVAEFERERIRERVFENMYHAASKGKWLTQHPFGYRLENKELVIYENEA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCEEEEEECCH
KIVRDIFIWYTQEGHGFYAIAKKLNLLGIPSRQNKQWSIRSVKLLLSNPVYKGTVVWNRK
HHEEEEEEEEEECCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCC
DRSKKKEQFKKEDEWVIQENAAPAIVTDELWNEAQKRMQTSTLAPRAQTSPHLLGGLLKC
HHHHHHHHHCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GKCGSGMSIGWSGSTKNRYRVYRCSANKNKGTCTSKQYKAHQLEELFKKGLSNLCESFSF
CCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SLHPSLSLQKRQTDVTNQMKLATAKKRYKRKVEAFTAGLIELEDLQEEKRRLETLELALQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTTELNENINLPQIEEWMKSKLKNIYDALSIVPVEELKPLIQTIIEKVILHSEEELEIVL
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHE
QPII
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]