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The map label for this gene is recJ1 [H]

Identifier: 218901966

GI number: 218901966

Start: 809345

End: 811024

Strand: Reverse

Name: recJ1 [H]

Synonym: BCAH820_0848

Alternate gene names: 218901966

Gene position: 811024-809345 (Counterclockwise)

Preceding gene: 218901967

Following gene: 218901965

Centisome position: 15.29

GC content: 35.12

Gene sequence:

>1680_bases
ATGAATACACATCATTTATCATCAACAAGCGGCAAATGGATTCCACATATTCAAATGCCGCATACTCCTCCTAATAAGGA
AGCTCTATTAACAATGGCAAAACATTTAAACATCCATCCAATATTTTTGTATCATTTACATCTTCATGGTTATGAGCAAT
TTGAAACAATTGAACGATTTCTTGTTCCAAATTGGGAACATTGCCATTCTCCGTTTCTTTTACAAGATATGAAAAAGGCG
GTATATCGAATTTTCAAAGCGATTCAAAACAAAGAAAACATCATGATCTTTGGTGATTATGATGCGGATGGTATTACGTC
TTCAACTTTATTATATAAAGGCTTAAAGAAGTTGAATGCCAATGTAACAGTACGACTTCCAATCCGTTCAGAAGGTTATG
GATTATCCGCAAAAGTGATGAATGAATTACCTTCAAACATTTCTCTTATTATTACAGTCGATAATGGTTCATCCGCCCAC
GAAGCTATGCAAGTTGCTAAACAAAAAGGAGTAGATGTCATTGTTACAGATCACCATGACATTTTACAAGGTTATCCTGA
TTGCTATGCATTCATTAATCCAAAAAGAAATGATGACACATATCCCTTTCCATTTCTTGCTGGAGCTGGCGTGGCACTCA
AAGTTGTACAAGCTCTATTTGAAACAATCAAACAACCCTTTTTAAAGTATTGTAGTGAATTAATTGAACTAGCAACAATC
GGAACTATTGCCGACTTGATGCCATTAAAAGACGAAAACCGTATTATCTGTTCATACGGATTAAAACAAATGAATAGTAA
TCCAAGCCCAGCTCTCAAAACACTATTCAAACTTTTAAAAATAAAAACAGTCGATAGTAGTACGATTGGATTCCAAATCG
GTCCACTTTTTAATGCAACAGGACGGATTGCAGATCCAAATATTACAGGTGAAATGCTTAGAAACAATGAAACAGATCAA
GCAAAATGGACTGAACTATTATGTATCAACAATCAACGTAAACAAATGACAAAAGAGCAGTTCCTAATCGCAGAAGAAAC
CATTTTCCAGGATCAGTTACATAAGGACAACGTAATTGTTGTTTGGGGAGATTTCCACCCTGGCATAATCGGATTGATTT
CATCTCGTATTTCCGAAAAATATAAAAAGCCTTCCATCGTTATCTCTAACACAGGGACAGGGTCTGCACGAAGCGTTAAT
AAAACAGACTTCTCTATCGTAAAAGTCATTCAAGATTGCCGAGACTATTTGAAAAAATACGGAGGGCATCGAGCAGCTGC
AGGATTATCAATACCAATGAAAGAACAGCATCTTCTTTTATTCCGTTCAGCTATTCAAGAAGCTGCAAAAACACAAACCT
GCCTAATTCCACCAACCTATTACTTATCGAAATTACCAATAGACAAATTTTCAGAACAGCTCTTTCGAGATTTGTACACA
TTAGAACCTTTCGGGATGGAAAATCCTAAGCCTATCTTCTTAAGTCAAAATACTAAACCTTCTTTTGTTAAAATGCTTGG
GGAAGATCATTTATTACTTGGAATTTCTTCAAGATGTGTATTTGGTTTTCAAAAAGGGGATTTGTATGAACAAATAAAAA
AAGCTCGAAGCATGGATTTTCTATATACAACAAATTGTTATCACCAACAGAACTTCTTAATTCAGCACCTTTCCTTATAA

Upstream 100 bases:

>100_bases
AAACCGCTCATACAAACGATTATTGAAAAAGTGATTCTACATAGCGAAGAAGAACTAGAAATTGTTTTACAACCTATTAT
ATAAATATGGAGGCTTCATC

Downstream 100 bases:

>100_bases
CATTTGTTAAACTTTCTTTTCAAACATTTATGAAATCAAGAGATAATGAATTTATATGGAATATATGCTAAAGGGGACAA
ACATACAAACAAAGTATTCA

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MNTHHLSSTSGKWIPHIQMPHTPPNKEALLTMAKHLNIHPIFLYHLHLHGYEQFETIERFLVPNWEHCHSPFLLQDMKKA
VYRIFKAIQNKENIMIFGDYDADGITSSTLLYKGLKKLNANVTVRLPIRSEGYGLSAKVMNELPSNISLIITVDNGSSAH
EAMQVAKQKGVDVIVTDHHDILQGYPDCYAFINPKRNDDTYPFPFLAGAGVALKVVQALFETIKQPFLKYCSELIELATI
GTIADLMPLKDENRIICSYGLKQMNSNPSPALKTLFKLLKIKTVDSSTIGFQIGPLFNATGRIADPNITGEMLRNNETDQ
AKWTELLCINNQRKQMTKEQFLIAEETIFQDQLHKDNVIVVWGDFHPGIIGLISSRISEKYKKPSIVISNTGTGSARSVN
KTDFSIVKVIQDCRDYLKKYGGHRAAAGLSIPMKEQHLLLFRSAIQEAAKTQTCLIPPTYYLSKLPIDKFSEQLFRDLYT
LEPFGMENPKPIFLSQNTKPSFVKMLGEDHLLLGISSRCVFGFQKGDLYEQIKKARSMDFLYTTNCYHQQNFLIQHLSL

Sequences:

>Translated_559_residues
MNTHHLSSTSGKWIPHIQMPHTPPNKEALLTMAKHLNIHPIFLYHLHLHGYEQFETIERFLVPNWEHCHSPFLLQDMKKA
VYRIFKAIQNKENIMIFGDYDADGITSSTLLYKGLKKLNANVTVRLPIRSEGYGLSAKVMNELPSNISLIITVDNGSSAH
EAMQVAKQKGVDVIVTDHHDILQGYPDCYAFINPKRNDDTYPFPFLAGAGVALKVVQALFETIKQPFLKYCSELIELATI
GTIADLMPLKDENRIICSYGLKQMNSNPSPALKTLFKLLKIKTVDSSTIGFQIGPLFNATGRIADPNITGEMLRNNETDQ
AKWTELLCINNQRKQMTKEQFLIAEETIFQDQLHKDNVIVVWGDFHPGIIGLISSRISEKYKKPSIVISNTGTGSARSVN
KTDFSIVKVIQDCRDYLKKYGGHRAAAGLSIPMKEQHLLLFRSAIQEAAKTQTCLIPPTYYLSKLPIDKFSEQLFRDLYT
LEPFGMENPKPIFLSQNTKPSFVKMLGEDHLLLGISSRCVFGFQKGDLYEQIKKARSMDFLYTTNCYHQQNFLIQHLSL
>Mature_559_residues
MNTHHLSSTSGKWIPHIQMPHTPPNKEALLTMAKHLNIHPIFLYHLHLHGYEQFETIERFLVPNWEHCHSPFLLQDMKKA
VYRIFKAIQNKENIMIFGDYDADGITSSTLLYKGLKKLNANVTVRLPIRSEGYGLSAKVMNELPSNISLIITVDNGSSAH
EAMQVAKQKGVDVIVTDHHDILQGYPDCYAFINPKRNDDTYPFPFLAGAGVALKVVQALFETIKQPFLKYCSELIELATI
GTIADLMPLKDENRIICSYGLKQMNSNPSPALKTLFKLLKIKTVDSSTIGFQIGPLFNATGRIADPNITGEMLRNNETDQ
AKWTELLCINNQRKQMTKEQFLIAEETIFQDQLHKDNVIVVWGDFHPGIIGLISSRISEKYKKPSIVISNTGTGSARSVN
KTDFSIVKVIQDCRDYLKKYGGHRAAAGLSIPMKEQHLLLFRSAIQEAAKTQTCLIPPTYYLSKLPIDKFSEQLFRDLYT
LEPFGMENPKPIFLSQNTKPSFVKMLGEDHLLLGISSRCVFGFQKGDLYEQIKKARSMDFLYTTNCYHQQNFLIQHLSL

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=482, Percent_Identity=29.6680497925311, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63399; Mature: 63399

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTHHLSSTSGKWIPHIQMPHTPPNKEALLTMAKHLNIHPIFLYHLHLHGYEQFETIERF
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHH
LVPNWEHCHSPFLLQDMKKAVYRIFKAIQNKENIMIFGDYDADGITSSTLLYKGLKKLNA
HCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
NVTVRLPIRSEGYGLSAKVMNELPSNISLIITVDNGSSAHEAMQVAKQKGVDVIVTDHHD
CEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCHH
ILQGYPDCYAFINPKRNDDTYPFPFLAGAGVALKVVQALFETIKQPFLKYCSELIELATI
HHCCCCCEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTIADLMPLKDENRIICSYGLKQMNSNPSPALKTLFKLLKIKTVDSSTIGFQIGPLFNAT
HHHHHHCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHEEECCCCCCCEEECCCCCCC
GRIADPNITGEMLRNNETDQAKWTELLCINNQRKQMTKEQFLIAEETIFQDQLHKDNVIV
CCCCCCCCCHHHHCCCCCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VWGDFHPGIIGLISSRISEKYKKPSIVISNTGTGSARSVNKTDFSIVKVIQDCRDYLKKY
EECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GGHRAAAGLSIPMKEQHLLLFRSAIQEAAKTQTCLIPPTYYLSKLPIDKFSEQLFRDLYT
CCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHHHHC
LEPFGMENPKPIFLSQNTKPSFVKMLGEDHLLLGISSRCVFGFQKGDLYEQIKKARSMDF
CCCCCCCCCCEEEEECCCCHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHHCCCE
LYTTNCYHQQNFLIQHLSL
EEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNTHHLSSTSGKWIPHIQMPHTPPNKEALLTMAKHLNIHPIFLYHLHLHGYEQFETIERF
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHH
LVPNWEHCHSPFLLQDMKKAVYRIFKAIQNKENIMIFGDYDADGITSSTLLYKGLKKLNA
HCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
NVTVRLPIRSEGYGLSAKVMNELPSNISLIITVDNGSSAHEAMQVAKQKGVDVIVTDHHD
CEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCHH
ILQGYPDCYAFINPKRNDDTYPFPFLAGAGVALKVVQALFETIKQPFLKYCSELIELATI
HHCCCCCEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTIADLMPLKDENRIICSYGLKQMNSNPSPALKTLFKLLKIKTVDSSTIGFQIGPLFNAT
HHHHHHCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHEEECCCCCCCEEECCCCCCC
GRIADPNITGEMLRNNETDQAKWTELLCINNQRKQMTKEQFLIAEETIFQDQLHKDNVIV
CCCCCCCCCHHHHCCCCCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VWGDFHPGIIGLISSRISEKYKKPSIVISNTGTGSARSVNKTDFSIVKVIQDCRDYLKKY
EECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GGHRAAAGLSIPMKEQHLLLFRSAIQEAAKTQTCLIPPTYYLSKLPIDKFSEQLFRDLYT
CCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHHHHC
LEPFGMENPKPIFLSQNTKPSFVKMLGEDHLLLGISSRCVFGFQKGDLYEQIKKARSMDF
CCCCCCCCCCEEEEECCCCHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHHCCCE
LYTTNCYHQQNFLIQHLSL
EEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]