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The map label for this gene is proS1 [H]

Identifier: 218901598

GI number: 218901598

Start: 430837

End: 432267

Strand: Direct

Name: proS1 [H]

Synonym: BCAH820_0440

Alternate gene names: 218901598

Gene position: 430837-432267 (Clockwise)

Preceding gene: 218901597

Following gene: 218901600

Centisome position: 8.12

GC content: 36.41

Gene sequence:

>1431_bases
ATGGCAAAAGAACAAGTACAAGCGATTACGAAGATGGAAGAGGACTTTGCACAGTGGTACACAGATATTGTAAAAAAAGC
AGAACTTGTTGATTATTCAAGTGTAAAAGGATGTATGATTCTACGTCCGTACGGCTATGCCTTATGGGAAAATATGCAGA
AAGTTATGGATGAGAAGTTAAAAGCAACTGGTCATGAAAATGTGTATATGCCAATGTTTATTCCAGAGAGCTTATTACAA
AAAGAGAAGGATCATGTAGAAGGATTTGCTCCTGAAGTAGCATGGGTGACACATGGCGGGGATGAAAAGTTAGCAGAAAG
ACTTTGTATACGCCCTACATCTGAAACTTTATTCTGTGAGCACTTTTCAAAAATTGTGCAATCCTATAATGATTTGCCAA
AGCTATATAATCAGTGGTGTTCAGTAGTTCGTTGGGAGAAAACAACTAGGCCATTCCTTCGCACAACAGAATTCTTATGG
CAAGAGGGACATACAATTCATGAAACAGCAGAAGAATCTCAAGCTGAAACGTTAAATATTTTAAATCTATATGCTTCTTT
CTGTGAAGACTATTTAGCGATACCGGTAATTAAAGGACAAAAAACAGAAAAAGAAAAATTTGCGGGAGCAAAGGCAACTT
ATACGATTGAAAGCTTAATGCATGATGGGAAAGCACTTCAAACAGGAACATCCCATAACTTTGGAACGAATTTCTCTGAA
GCATTTGATATTAAATTTTTAGATCGTACCGGTAAGTGGCAATATGTACACCAAACATCTTGGGGCGTATCAACGAGAAT
GATAGGTGGACTTATTATGGTTCATAGTGATAATAATGGGCTTGTAATGCCTCCTAAAGTCGCACCAGTGCAAGTTGTTA
TTGTACCGATTGCGCAGCATAAAGAAGGAGTTTTGGCGAAAGCGATAGAGTTACAAGGACATATTCAAAAGGTTGCACGA
GTAAAAATAGATGCTAGTAATAAAACACCAGGTTGGAAATTTAATGAGTATGAAATGAAAGGTATTCCAATTCGATTAGA
AGTTGGTCCTAAAGATATTGAAAAGAATCAAGTTGTACTCGTAAGAAGAGATACGAAAGAAAAAGAGTTTATATCAATGG
ATCAATTAGAAGAACGCATTCCAGCACTACTTGAGGAAATTCATAACTCCTTATTTAATAAAGCAAAAGTATTTCGAGAT
GAAAATACGTATAGTGTGACGAGTTTTGAAGAAATGAAAAAAGTAGCTGATGAAAAGCAAGGATTTATTAAGGCAATGTG
GTGCGGAGAAGTAGCTTGTGAAGAGAAGCTAAAAGAAGAAGTTGGAGTATCTTCACGCTGTATGCCTTTTGAACAAGAAC
ATTTAGCAGATGAATGTGTTTGTTGTGGGAAGGAAGCTAAACAAATGGTTTATTGGGGAAAAGCGTATTAA

Upstream 100 bases:

>100_bases
CAATTTTTGTGCATGAATCAGGGTGGTAACGCCGGCAAAGCTCGGTCCCTATTTAGGGACGCGGGCTTTTTTGTATTTTC
TAGAAGGAGGAAGACTGACT

Downstream 100 bases:

>100_bases
TGCTCTTTTTGATAGATAATGAAAAGGACTTTGGGTGCATAAAAAAATGCAATCCAAAGTCCTTTTAGTTTTATAATTTA
TTTCATGTATTTTTTAGATG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 2; ProRS 2 [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATGHENVYMPMFIPESLLQ
KEKDHVEGFAPEVAWVTHGGDEKLAERLCIRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFDIKFLDRTGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKAIELQGHIQKVAR
VKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRD
ENTYSVTSFEEMKKVADEKQGFIKAMWCGEVACEEKLKEEVGVSSRCMPFEQEHLADECVCCGKEAKQMVYWGKAY

Sequences:

>Translated_476_residues
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATGHENVYMPMFIPESLLQ
KEKDHVEGFAPEVAWVTHGGDEKLAERLCIRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFDIKFLDRTGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKAIELQGHIQKVAR
VKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRD
ENTYSVTSFEEMKKVADEKQGFIKAMWCGEVACEEKLKEEVGVSSRCMPFEQEHLADECVCCGKEAKQMVYWGKAY
>Mature_475_residues
AKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATGHENVYMPMFIPESLLQK
EKDHVEGFAPEVAWVTHGGDEKLAERLCIRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQ
EGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSEA
FDIKFLDRTGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKAIELQGHIQKVARV
KIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDE
NTYSVTSFEEMKKVADEKQGFIKAMWCGEVACEEKLKEEVGVSSRCMPFEQEHLADECVCCGKEAKQMVYWGKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=495, Percent_Identity=45.2525252525253, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI1786392, Length=159, Percent_Identity=26.4150943396226, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=43.0612244897959, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=47.0588235294118, Blast_Score=353, Evalue=8e-98,
Organism=Saccharomyces cerevisiae, GI6321808, Length=501, Percent_Identity=47.5049900199601, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24649468, Length=494, Percent_Identity=43.3198380566802, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24649466, Length=494, Percent_Identity=43.3198380566802, Blast_Score=411, Evalue=1e-115,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54640; Mature: 54509

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KATGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGDEKLAERLCIRPTSETLFCE
HHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQAETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
HHHHHHHHHHHHCCEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCC
AFDIKFLDRTGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEECCCC
KEGVLAKAIELQGHIQKVARVKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVL
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDENTYSVTSFEEMKKVADEKQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHC
GFIKAMWCGEVACEEKLKEEVGVSSRCMPFEQEHLADECVCCGKEAKQMVYWGKAY
CCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHEEECCCCC
>Mature Secondary Structure 
AKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KATGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGDEKLAERLCIRPTSETLFCE
HHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQAETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
HHHHHHHHHHHHCCEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCC
AFDIKFLDRTGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEECCCC
KEGVLAKAIELQGHIQKVARVKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVL
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDENTYSVTSFEEMKKVADEKQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHC
GFIKAMWCGEVACEEKLKEEVGVSSRCMPFEQEHLADECVCCGKEAKQMVYWGKAY
CCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]