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The map label for this gene is kinE [H]

Identifier: 218901525

GI number: 218901525

Start: 354350

End: 356368

Strand: Direct

Name: kinE [H]

Synonym: BCAH820_0367

Alternate gene names: 218901525

Gene position: 354350-356368 (Clockwise)

Preceding gene: 218901524

Following gene: 218901526

Centisome position: 6.68

GC content: 35.31

Gene sequence:

>2019_bases
ATGTTAAGTGATCAAACGAGATATTCTAGGCTCGCGAATATAACCAAAATAATAAATACAAAATTAGAACTACGTGAAGT
ATTGCAGCGTGTAACAATGGCGATATCAGAGGAGATTGTTAGGTGTGACGCTGTTGGAATTTATTTACCTGAAGAAGATG
GAACATTTAGAGGGTTTGCAGGAAAACCAGAGACCATAAATGGCGTAACGCTCGATACGCAGGTAATTGACCTTGAAGTA
GACTTACTGGCAAAAGAAGTTATTGAAACTAAAAAAACCATCTATATTCCTGATACCTCAAAGGATCATCGTCCGGATCC
GAGACCAGTCGATGCGTTCAAAATTAAGTCTTTATTAGCTCTGCCTATCTCATTTGGGCAAGAGTTATTTGGTCTTGTTT
TTTTATTTGATTATGGAGTTCCAATGAACTTAACAGGTTCAGAAATTCAAAGTGTTGAAGCTTATGTAAACATGGCGGCG
GTCGCCATTCAAAACGCAAAAAATTTAACCCAAAAAGAAAATCTTATTGCCGAGAAGCAGCTGTTACTAAATGTTACCCG
TGATTTATCAATGTGTTCCTCAATACAGGAGAGTTTTGATAAATGCTTTTTTTACTTAGGACAGATTTTAGAGAGTAAAA
ACATGGCTGCCCACCTTTTAGATCCGCTAGACAAAACAACGATTAAGACAACGGAGTTAAGCTCGGGCTGTGATTGGATA
GAAGCAGATTGGATGGAGAAAAGCTATGAAGCCAAGATTCAAGAGATTATTCGAACAAAACATATAGATAGTAAGGGTTT
ACTGATGATTCCATTGGTTTCAATGGGAGAAGTATTAGGGGTAATCGTCGTTGGCAAAGAGGAAAAAACTCATAATTACG
ATGATTCCCGAATTCAACTGGCAAAATCTATCGTTGATGCTACAGCCCCTACATTTTCAAACTTGTTATATATGGATCAA
CTTGAAAGCATGGTGGAAGAACGAACGAGAGAACTAGCTGCTGCTAATGAAAAAGTGACAAGTGTGATTGAAAGTATTAC
GGATGGATTCTTGACTTTGAATAATAAATGGGAATTTACGTATGTAAATAAACATCAACATTTCCCACAAAGAAAAACAG
CAAAAGACGTATTAGGTAAGAATATATGGGAGGTCTTCCCAAGTAGCGTAGATACAGTTATATATAAGGAGCTTCATCGT
GTAATGTCAGAGCGAACTACAGTTCATTTTGAATTTTTTTCTACATTTGATGAATATTGGCATGAAGTTATTGCATATCC
GTATGATGATGGTATTTGTTGTATTTGTAAAAACATAACAGAAAAAAAGCAATATGAGCAGGAATTGAAAAGGTTATCCA
ACATAGAGTTAATAGGACAAATGGCAGCAGGTATCAGCCACGAGATTAGAAATCCAATGACAACAATACGAGGATTTTTG
CAGTTATTAAAAGAAGAGAATAACTATGAGAAACATAATAACTACTTTAATTTAATGGTTGAAGAACTTGACCGTGCCAA
TTCTATTATTACTGAATTTCTCTCAATAGGTAATACAAAGAAATCAGATTTGCAGATGCTAGATTTAAACTCAATTATCC
ACGATATTATTCCTTTAATAAAGATAGATACGCATAATCAAAATAAATATATTCAAGTCCATACAAATGACGTTCCAGCA
TTACTTTTAAATCGTAATGAGATAAGGCAATTATTAATGAACCTATACCGTAATGGCTTAGAAGCGATGAGTACAGGGAA
AACTCTAACCATTAGCACCTATAAAGAAGGGCAAAATTGTGTGGTGCTTGCAGTGCGGGATGAAGGGAAAGGTATTAGCC
CTGAAGTATTAGAGAAACTGGGGACTCCATTTTACACAACCAAAGAGAATGGAACTGGATTGGGATTAGGCGTATGTTAT
GCCATTGCTACCCGTCATAATGCAAAAATAGAAATTCAAACAGGATCGGAAGGCACTACATTTTTTGTTAAATTTAATTA
TGAAAATAATAAAAAATAA

Upstream 100 bases:

>100_bases
GAGGTTAGGTTGGATTCAGGCTGATAATAAGTGGGGGGATGAATCACCCCTCACTTATTAAAGTTGCACTTTATTACGCC
TATAATTGGAGGCTATGCAA

Downstream 100 bases:

>100_bases
TCTTATTTAGATTAGTTAAGGTAAACTGTATTACAAATAAAAAGTTAAAAATAATCAACATTTCAGGAGGAAAAACAATG
GCAATGAGCAACAACGATAT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGTFRGFAGKPETINGVTLDTQVIDLEV
DLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKIKSLLALPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAYVNMAA
VAIQNAKNLTQKENLIAEKQLLLNVTRDLSMCSSIQESFDKCFFYLGQILESKNMAAHLLDPLDKTTIKTTELSSGCDWI
EADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDDSRIQLAKSIVDATAPTFSNLLYMDQ
LESMVEERTRELAAANEKVTSVIESITDGFLTLNNKWEFTYVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIYKELHR
VMSERTTVHFEFFSTFDEYWHEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTTIRGFL
QLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHDIIPLIKIDTHNQNKYIQVHTNDVPA
LLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYKEGQNCVVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLGLGVCY
AIATRHNAKIEIQTGSEGTTFFVKFNYENNKK

Sequences:

>Translated_672_residues
MLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGTFRGFAGKPETINGVTLDTQVIDLEV
DLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKIKSLLALPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAYVNMAA
VAIQNAKNLTQKENLIAEKQLLLNVTRDLSMCSSIQESFDKCFFYLGQILESKNMAAHLLDPLDKTTIKTTELSSGCDWI
EADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDDSRIQLAKSIVDATAPTFSNLLYMDQ
LESMVEERTRELAAANEKVTSVIESITDGFLTLNNKWEFTYVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIYKELHR
VMSERTTVHFEFFSTFDEYWHEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTTIRGFL
QLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHDIIPLIKIDTHNQNKYIQVHTNDVPA
LLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYKEGQNCVVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLGLGVCY
AIATRHNAKIEIQTGSEGTTFFVKFNYENNKK
>Mature_672_residues
MLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGTFRGFAGKPETINGVTLDTQVIDLEV
DLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKIKSLLALPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAYVNMAA
VAIQNAKNLTQKENLIAEKQLLLNVTRDLSMCSSIQESFDKCFFYLGQILESKNMAAHLLDPLDKTTIKTTELSSGCDWI
EADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDDSRIQLAKSIVDATAPTFSNLLYMDQ
LESMVEERTRELAAANEKVTSVIESITDGFLTLNNKWEFTYVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIYKELHR
VMSERTTVHFEFFSTFDEYWHEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTTIRGFL
QLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHDIIPLIKIDTHNQNKYIQVHTNDVPA
LLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYKEGQNCVVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLGLGVCY
AIATRHNAKIEIQTGSEGTTFFVKFNYENNKK

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=30.5676855895196, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1788549, Length=245, Percent_Identity=26.530612244898, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790300, Length=233, Percent_Identity=26.6094420600858, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI48994928, Length=359, Percent_Identity=20.891364902507, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789808, Length=210, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=252, Percent_Identity=20.6349206349206, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790346, Length=214, Percent_Identity=25.7009345794392, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1788393, Length=201, Percent_Identity=26.3681592039801, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76466; Mature: 76466

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGTFRGFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC
GKPETINGVTLDTQVIDLEVDLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKIKSLLA
CCCCCCCCEEEEEEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
LPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAYVNMAAVAIQNAKNLTQKENLIAEKQ
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLNVTRDLSMCSSIQESFDKCFFYLGQILESKNMAAHLLDPLDKTTIKTTELSSGCDWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCCH
EADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDDSRIQL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCCCHHHHHH
AKSIVDATAPTFSNLLYMDQLESMVEERTRELAAANEKVTSVIESITDGFLTLNNKWEFT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
YVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIYKELHRVMSERTTVHFEFFSTFDEYW
EECCCCCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
HEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTTIRGFL
HHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHDIIPLI
HHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHE
KIDTHNQNKYIQVHTNDVPALLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYKEGQNC
EEECCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCE
VVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLGLGVCYAIATRHNAKIEIQTGSEGTT
EEEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCE
FFVKFNYENNKK
EEEEEECCCCCC
>Mature Secondary Structure
MLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGTFRGFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC
GKPETINGVTLDTQVIDLEVDLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKIKSLLA
CCCCCCCCEEEEEEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
LPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAYVNMAAVAIQNAKNLTQKENLIAEKQ
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLNVTRDLSMCSSIQESFDKCFFYLGQILESKNMAAHLLDPLDKTTIKTTELSSGCDWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCCH
EADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDDSRIQL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCCCHHHHHH
AKSIVDATAPTFSNLLYMDQLESMVEERTRELAAANEKVTSVIESITDGFLTLNNKWEFT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
YVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIYKELHRVMSERTTVHFEFFSTFDEYW
EECCCCCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
HEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTTIRGFL
HHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHDIIPLI
HHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHE
KIDTHNQNKYIQVHTNDVPALLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYKEGQNC
EEECCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCE
VVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLGLGVCYAIATRHNAKIEIQTGSEGTT
EEEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCE
FFVKFNYENNKK
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]