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The map label for this gene is dus2 [H]

Identifier: 218901524

GI number: 218901524

Start: 353044

End: 354033

Strand: Direct

Name: dus2 [H]

Synonym: BCAH820_0366

Alternate gene names: 218901524

Gene position: 353044-354033 (Clockwise)

Preceding gene: 218901523

Following gene: 218901525

Centisome position: 6.66

GC content: 41.11

Gene sequence:

>990_bases
ATGATAGATAATTTTTGGCGTGATTTACCACGACCATTTTTTGTACTTGCACCAATGGAAGATGTGACAGACGTTGTGTT
TCGTCACGTAGTAAGTGAGGCTGGCCGTCCGGACGTATTCTTCACAGAGTTCACAAATTCGGATAGCTACTGTCATCCAG
AAGGTATGAAAAGCGTACGTGGCCGTTTAATTTTTACAGAAGACGAACAGCCAATGGTGGCACATATTTGGGGAGATAAC
CCTGAATATTTCCGTCAAATGAGTATTGGTATGGCAGAACTAGGATTTAAAGGCATCGATATTAATATGGGTTGCCCAGT
ACCGAACGTTGCATCAAGAGGAAAAGGTAGTGGTCTTATTCTACGTCCAGACGTTGCGGCAGAACTTATTCAAGCTGCAA
AAGCAGGCGGACTACCTGTCAGCGTAAAAACACGACTTGGCTTTAAAGAGTTAAGCGAGTGGGAAGATTGGTTAACGCAC
ATTTTCAAACAAGATATTGCAAACCTTTCTATTCATTTACGCACAAGAGAAGAAATGAGCCAAGTAGATGCGCACTGGGA
ACTAATTCCGGAAATTAAAAAATTACGTGATCGCATTGCACCAAATACGCTCCTAACAATCAACGGAGACATCCTTGATC
GTAAAATGGGGCTGGAACTTGCTGAAAAATATGGTATTGATGGCGTAATGATTGGACGAGGAATCTTTAAAAATCCATTT
GCGTTTGAAAAAGAGCCAAGAGAGCATAGCAGTAAAGAACATCTAGATCTTTTAAGACTGCAGCTTGATCTTCAAGATCA
ATATGCAGAAGTATTACCACGATCCATAACGGGATTACATCGCTTCTTCAAAATTTATGTAAAAGGCTTCCCTGGGGCTG
CTGAACTAAGAAATCAATTGATGAGCACGAAGTCAACGGATGAAGTACGTGCATTGCTTAATAAGTTTGAGGAGAGTGTT
AATGTGGTAGAAGGTAGTGAAACGGTGTAA

Upstream 100 bases:

>100_bases
ATAATAAAAAAGCAGTTGATATAGGAAAATCTATACCATCTGCTTTTTGGTTTATTGAGAGTTTGAAATTGTATTCATAG
TGAGAGGATTTGAATTTGAA

Downstream 100 bases:

>100_bases
CTGTTGAAAGTTAGAGGGGCTGGAGTGTAGTAATTTTATTAGTGTAAGGTGAACCGTATCATAAGTAAGTGAGGTATTCT
ATATAAAACAAGGGTAAGAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLNKFEESV
NVVEGSETV

Sequences:

>Translated_329_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLNKFEESV
NVVEGSETV
>Mature_329_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLNKFEESV
NVVEGSETV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=334, Percent_Identity=24.5508982035928, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI239788483, Length=174, Percent_Identity=32.183908045977, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI239788462, Length=174, Percent_Identity=32.183908045977, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=28.75, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789660, Length=323, Percent_Identity=26.9349845201238, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693211, Length=338, Percent_Identity=25.1479289940828, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=176, Percent_Identity=31.8181818181818, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=26.7605633802817, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=322, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921524, Length=236, Percent_Identity=27.9661016949153, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24585320, Length=265, Percent_Identity=26.4150943396226, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37402; Mature: 37402

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHC
GRLIFTEDEQPMVAHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLI
CEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCCEEEECCCCCCCHHCCCCCCCEE
LRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTHIFKQDIANLSIHLRTREEMS
ECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHH
QVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQL
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MSTKSTDEVRALLNKFEESVNVVEGSETV
HCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHC
GRLIFTEDEQPMVAHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLI
CEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCCEEEECCCCCCCHHCCCCCCCEE
LRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTHIFKQDIANLSIHLRTREEMS
ECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHH
QVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQL
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MSTKSTDEVRALLNKFEESVNVVEGSETV
HCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]