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The map label for this gene is tilS [H]

Identifier: 218901265

GI number: 218901265

Start: 67558

End: 68988

Strand: Direct

Name: tilS [H]

Synonym: BCAH820_0071

Alternate gene names: 218901265

Gene position: 67558-68988 (Clockwise)

Preceding gene: 218901264

Following gene: 218901266

Centisome position: 1.27

GC content: 36.41

Gene sequence:

>1431_bases
TTGAAAGATACATTTGTTGAAAAGGTAGATGACTTTGTAAGGCAACATGATGTATTAAAGGAACGTTCAACAATTGTTGT
AGGGGTTTCTGGTGGTCCTGACTCTTTGGCTCTTCTATATTATTTATTAGAAAAAAGAGCAGCAAAACAGTTTGAAATTG
TAGTGGCGCATGTGGATCATATGTTTAGAGGTGATGAATCTCATGAGGACCTACAGTTTGTGCAGGACCTTTGTAAAGGA
CTGGGAGTTATTTGCGAAACGATAAGGATTAATGTGTCGCAATATCAAAAGCAATACGGGATGAATGCACAGGTTGCTGC
TAGAGAATGCAGATATGCATTTTTGGAAAGAATAATGAAGAAATATGATGCGAGATATGTAGCCCTTGGACATCATGGTG
ATGATCAAGTAGAGACGATTTTAATGCGTCTTGTACGAGGGAGTACTCCGAAAGGATATGCAGGAATTGCAGTGAAGCGT
CCTTTTCATAATGGGTATTTAATTAGGCCGTTACTTGGAGTGACGAAGGAAGAAATTGTTAATTACTGTAATGAGCTAAA
AATAATTCCTCGTATAGATCCAAGTAATAAAAAGGAAGTATATACAAGGAATCGATTACGTAAATATGTTCTTCCTCATT
TGAAAGAAGAAAACCCACAAGTGCATGAGAAATTCCAAAAATTTAGCATGCAGATGCAAGAGGATGAGGCGTATTTGCAG
GAATTAGCTTTTGAGAAAATGAATAAAGTAATTACAAAAAAAAGTGATAAACAAATTAGCTTATCAATTCCTGCCTTTGA
ATCCATGTCTATGCCTTTACAAAGAAGAGGGATTCAACTAATATTAAACTATCTTTATGAATATAAGATTCCATCTTCTC
TTTCCTCTATACATATTGACAAGGTGATTGAGTTTTTTAAGCGGACACAACCTTCAGGTTCACTTGATTTCCCAGGTGAT
TTGAAAATTGTTCGCGCGTACGAGGAGTGTAGCTTTGGATTTAAACAAGAAATTGTTTCCCCTTTTTTACAAGACTTATC
AGTGCCCGGGACAATTACGCTATCGAACGGGGATAAACTTGTAACAGAGGTGAGCGAAGATATACCAAGTGACATGAATG
AAACAGTATTTGTTGCTAAGTATAATGATATATCATATCCAATTCGTATTCGGTCTAGAGAAAATGGAGATCGCATGTCA
ATACAAGGTATGAATGGCACGAAAAAGATAAAAGCTATTTTTATCGAAGCAAAAGTACCAAGAGAAAAAAGAGAAGAATG
GCCGGTCGTTTGTGATGCAAGTGGGAATATTATTTGGTTACCCTTGTTGAAGCGATCTGCATTTGCTATCTCGAAAGAGA
CAGCAAAGAAGGATAAATATATGATTATTCACTACAAAAGCAAGGAGTCTTCCGGGAGGATAATGAAATGA

Upstream 100 bases:

>100_bases
TTTGTATATCTTTTTTTTGCACAATGTTTGTAAAAGTTGTACCATTATAAACAAGGTAACTAACGTTGCTGTTCTTGTTT
AATGAAAGGTGATTACGAAG

Downstream 100 bases:

>100_bases
TGAATCAAGATATCGAAAAAGTATTAATTTCTGAAGAGCAAATACAAGAAAAGGTGCTCGAATTAGGTACGATTATTGCA
GAGGATTATAAAAACACAGT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK

Sequences:

>Translated_476_residues
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
>Mature_476_residues
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=421, Percent_Identity=27.3159144893112, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54862; Mature: 54862

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDH
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRGDESHEDLQFVQDLCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMK
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIV
HHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCHHHHH
NYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
HHHHHCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KVIEFFKRTQPSGSLDFPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKL
HHHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCHH
VTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMSIQGMNGTKKIKAIFIEAKVP
HHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCC
REKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
HHHHCCCCEEEECCCCEEEEEEHHCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDH
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRGDESHEDLQFVQDLCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMK
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIV
HHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCHHHHH
NYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
HHHHHCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KVIEFFKRTQPSGSLDFPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKL
HHHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCHH
VTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMSIQGMNGTKKIKAIFIEAKVP
HHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCC
REKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
HHHHCCCCEEEECCCCEEEEEEHHCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]