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The map label for this gene is nadE [H]

Identifier: 218884778

GI number: 218884778

Start: 1358175

End: 1359017

Strand: Reverse

Name: nadE [H]

Synonym: DKAM_1467

Alternate gene names: 218884778

Gene position: 1359017-1358175 (Counterclockwise)

Preceding gene: 218884780

Following gene: 218884775

Centisome position: 99.55

GC content: 44.96

Gene sequence:

>843_bases
ATGGTGCCGGATTTGAAGCACATCTCCATTGAGGATTTATTAGCGATACCATTTGATGTCGTAGCTAGATCGATAGCAGA
TTTTATTGAGAAATATTTCAATGATTCTGGAGCAAAGAAAGCCATTATTGGTTTAAGCGGTGGACTTGACTCGTCTGTAA
CACTGAGGCTACTTGTTGACGCGGTTGGATCGGACCGGGTTATCGGAGTCATAATGCCTGATACAAGGGTGACACCTACC
GAAGACGTAGATGATGCAGTGGAATTATCAAGAGATCTCGGCGTGGAATACATGATCATACCAATCGATGACGTGGTCAA
CTCTTATAGAGTTATACCAGGCTTCGATGTAAACGAAAAATTAAGCACCGGGAATCTGAGAGCCAGGATTAGGATGAATA
TTTTATACTATTTAGCAAACAAGTTGAATGGTGTGGTGATCGGCACAGGGGATAGAAGCGAGATTCTCCTAGGGTACTTC
ACAAAATATGGTGATGGAGGCGTGGACGTATTGCCCATTGCATGCCTATACAAGACACAGGTCAAGGAGATGGGTAGGTA
TCTTGGGCTACCTGTAAGAATAACCAGTAAGCCGAGCTCACCGAGACTATGGAGGGATCATCTGGCTGAAGAGGAACTGG
GTTTATCATATAACATGGTAGACCTAGTATTCTACTCGTTGATCGATAAGGGGTTAAGCACGGAAGAAGCCGTTGATGCG
ACCGGCCTACCCCTTAACGTGTTTGAAAAAGTTCTAACGCTACATAGGAGAAGCCGGCATAAGAGGAGGCTACCGCCCAT
ACCTAGTCTACCATGGCTTCCCCAACCAATTAGAGAGATCTAA

Upstream 100 bases:

>100_bases
ACCACGATACTAATTGATCAGGTATATTTATGAAAATCAATCCTCAAATACACTGTTTACAAGCAAGTCCTTTTAAGCTT
CGACGAGACAGATTATTTAA

Downstream 100 bases:

>100_bases
CCTTAATGATTACATCGAGAAACGATTGTTTAACCTCTATGTAAGAGTTTTCAAACACTAGCCTTGCATTCAATGGATGA
GTGCTTAGACTTATTATAGA

Product: NAD+ synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MVPDLKHISIEDLLAIPFDVVARSIADFIEKYFNDSGAKKAIIGLSGGLDSSVTLRLLVDAVGSDRVIGVIMPDTRVTPT
EDVDDAVELSRDLGVEYMIIPIDDVVNSYRVIPGFDVNEKLSTGNLRARIRMNILYYLANKLNGVVIGTGDRSEILLGYF
TKYGDGGVDVLPIACLYKTQVKEMGRYLGLPVRITSKPSSPRLWRDHLAEEELGLSYNMVDLVFYSLIDKGLSTEEAVDA
TGLPLNVFEKVLTLHRRSRHKRRLPPIPSLPWLPQPIREI

Sequences:

>Translated_280_residues
MVPDLKHISIEDLLAIPFDVVARSIADFIEKYFNDSGAKKAIIGLSGGLDSSVTLRLLVDAVGSDRVIGVIMPDTRVTPT
EDVDDAVELSRDLGVEYMIIPIDDVVNSYRVIPGFDVNEKLSTGNLRARIRMNILYYLANKLNGVVIGTGDRSEILLGYF
TKYGDGGVDVLPIACLYKTQVKEMGRYLGLPVRITSKPSSPRLWRDHLAEEELGLSYNMVDLVFYSLIDKGLSTEEAVDA
TGLPLNVFEKVLTLHRRSRHKRRLPPIPSLPWLPQPIREI
>Mature_280_residues
MVPDLKHISIEDLLAIPFDVVARSIADFIEKYFNDSGAKKAIIGLSGGLDSSVTLRLLVDAVGSDRVIGVIMPDTRVTPT
EDVDDAVELSRDLGVEYMIIPIDDVVNSYRVIPGFDVNEKLSTGNLRARIRMNILYYLANKLNGVVIGTGDRSEILLGYF
TKYGDGGVDVLPIACLYKTQVKEMGRYLGLPVRITSKPSSPRLWRDHLAEEELGLSYNMVDLVFYSLIDKGLSTEEAVDA
TGLPLNVFEKVLTLHRRSRHKRRLPPIPSLPWLPQPIREI

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=263, Percent_Identity=26.2357414448669, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 31193; Mature: 31193

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPDLKHISIEDLLAIPFDVVARSIADFIEKYFNDSGAKKAIIGLSGGLDSSVTLRLLVD
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHEE
AVGSDRVIGVIMPDTRVTPTEDVDDAVELSRDLGVEYMIIPIDDVVNSYRVIPGFDVNEK
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHCEEECCCCCCCCC
LSTGNLRARIRMNILYYLANKLNGVVIGTGDRSEILLGYFTKYGDGGVDVLPIACLYKTQ
CCCCCEEEEEHHHHHHHHHHHCCEEEEECCCCCHHHEEHHHHCCCCCCCHHHHHHHHHHH
VKEMGRYLGLPVRITSKPSSPRLWRDHLAEEELGLSYNMVDLVFYSLIDKGLSTEEAVDA
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHCC
TGLPLNVFEKVLTLHRRSRHKRRLPPIPSLPWLPQPIREI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
>Mature Secondary Structure
MVPDLKHISIEDLLAIPFDVVARSIADFIEKYFNDSGAKKAIIGLSGGLDSSVTLRLLVD
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHEE
AVGSDRVIGVIMPDTRVTPTEDVDDAVELSRDLGVEYMIIPIDDVVNSYRVIPGFDVNEK
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHCEEECCCCCCCCC
LSTGNLRARIRMNILYYLANKLNGVVIGTGDRSEILLGYFTKYGDGGVDVLPIACLYKTQ
CCCCCEEEEEHHHHHHHHHHHCCEEEEECCCCCHHHEEHHHHCCCCCCCHHHHHHHHHHH
VKEMGRYLGLPVRITSKPSSPRLWRDHLAEEELGLSYNMVDLVFYSLIDKGLSTEEAVDA
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHCC
TGLPLNVFEKVLTLHRRSRHKRRLPPIPSLPWLPQPIREI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA