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The map label for this gene is rpoA2 [H]

Identifier: 218884684

GI number: 218884684

Start: 1271922

End: 1273142

Strand: Reverse

Name: rpoA2 [H]

Synonym: DKAM_1373

Alternate gene names: 218884684

Gene position: 1273142-1271922 (Counterclockwise)

Preceding gene: 218884685

Following gene: 218884683

Centisome position: 93.26

GC content: 44.64

Gene sequence:

>1221_bases
ATGCAGGGTTTAACTATAGAGGAAATCCAACAGCTCCTCGAGGAGAGACTGAAGGAGAACGTTAGCCAGCAGGTATATGA
GGAAGTACGTAATAAATTATTGAACATAGCTGGGAAGATCGTGATAACTAGGGAAACCGTAGAAAGATTCATAGAACATA
TAATCGAGAGATATCATTCCTCGTTGATAGAGCCAGGCGAGGCTGTGGGAACTATTGCCGCCCAAAGCCTTGGAGAGCCA
TCAACACAGATGACATTAAGGGTATTCCACTATGCCGGTGTACGTGAATACAACGTAACCCTCGGTCTCCCCAGGCTCAT
AGAGATAGTTGACGCCAGGAAAAAGCCTGAAACACCCATCATGGAGATATATCTAGTGGATGAATATAGGAGAAGCGAGG
AGAAGGCTAGGGAGATAGCTAGAAAAATAGAGTCAACATACCTAGAGAACATCACCAGGGAACTAGATATCGACCTCATA
GAAGGTGTGACGAGGGTACGTCTGGATCCCGAGATGCTGGAGGATAAGGGATTAAGTGTCGAGGTGATCATTAAGAAGTT
GAAGGAGCTAGATGTGGGCGAGGTTTCAAGAGGAGATGACCCATACGAGGTAATAATATCGCTTAGAGAGGAATACCTCG
ACTACAGTAAGCTAGAGAAGCTGAGAAGCAAACTTCTCGGTCTGCATTTGAAGGGTATAAAGGAGATACGTAAGGTAATA
ATCCAGAAACGCGGCGACGAGTACGTGATAATATCTGAGGGAAGCAACCTTGAGGAAATAATGAGGATAGAAGGCGTGGA
CTGGAGGAGAGTGTATACTAATAATATACATGAGATAGAAAGAGTGCTCGGTATAGAGGCCGCTAGGCAAGCTATAATCA
AGGAGATCAAAGAGATACTTGATGACCAGGGTCTAGACGTAGATATAAGGCATATAATGCTTCTAGCCGATATGATGACG
TGGACCGGGCATGTGAGGCAGATTGGTAGAATGGGTGTTGCCGGTGAAAAGCCAAGCGTACTGGCCAGGGCTACATTCGA
GATGACTGTTCAAAAGCTCCTAGAATCAGCAGCCACTAGTGAGGAAGACCGTTTATATGGCGTTACAGAAAATATTATAA
TAGGTCAAACAATACCTGTAGGAACTGGTATGGTTCAGATTTATATGACTCCAAGCCTATTAAAGACCGAGGCTGGCAAC
AAAGGTGATAGGAATGAGTAG

Upstream 100 bases:

>100_bases
CTCTATGGTGAAGATGGTGTTGACCCGATGAAGAGTGACCATGGTAAATCCGTTAACGTAGATAGAGTCATTGAGAAAAT
ATTTGGAGGTGGACTATAAG

Downstream 100 bases:

>100_bases
CCAGGTTGACCTGCTTAAAGCGATAAGAACGACTGCTGATACAGGGCAAATAGTGTATGGAAGTAGGCAAGTCAGGAGAC
TAGTTCTTCACGGGAAGGCT

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MQGLTIEEIQQLLEERLKENVSQQVYEEVRNKLLNIAGKIVITRETVERFIEHIIERYHSSLIEPGEAVGTIAAQSLGEP
STQMTLRVFHYAGVREYNVTLGLPRLIEIVDARKKPETPIMEIYLVDEYRRSEEKAREIARKIESTYLENITRELDIDLI
EGVTRVRLDPEMLEDKGLSVEVIIKKLKELDVGEVSRGDDPYEVIISLREEYLDYSKLEKLRSKLLGLHLKGIKEIRKVI
IQKRGDEYVIISEGSNLEEIMRIEGVDWRRVYTNNIHEIERVLGIEAARQAIIKEIKEILDDQGLDVDIRHIMLLADMMT
WTGHVRQIGRMGVAGEKPSVLARATFEMTVQKLLESAATSEEDRLYGVTENIIIGQTIPVGTGMVQIYMTPSLLKTEAGN
KGDRNE

Sequences:

>Translated_406_residues
MQGLTIEEIQQLLEERLKENVSQQVYEEVRNKLLNIAGKIVITRETVERFIEHIIERYHSSLIEPGEAVGTIAAQSLGEP
STQMTLRVFHYAGVREYNVTLGLPRLIEIVDARKKPETPIMEIYLVDEYRRSEEKAREIARKIESTYLENITRELDIDLI
EGVTRVRLDPEMLEDKGLSVEVIIKKLKELDVGEVSRGDDPYEVIISLREEYLDYSKLEKLRSKLLGLHLKGIKEIRKVI
IQKRGDEYVIISEGSNLEEIMRIEGVDWRRVYTNNIHEIERVLGIEAARQAIIKEIKEILDDQGLDVDIRHIMLLADMMT
WTGHVRQIGRMGVAGEKPSVLARATFEMTVQKLLESAATSEEDRLYGVTENIIIGQTIPVGTGMVQIYMTPSLLKTEAGN
KGDRNE
>Mature_406_residues
MQGLTIEEIQQLLEERLKENVSQQVYEEVRNKLLNIAGKIVITRETVERFIEHIIERYHSSLIEPGEAVGTIAAQSLGEP
STQMTLRVFHYAGVREYNVTLGLPRLIEIVDARKKPETPIMEIYLVDEYRRSEEKAREIARKIESTYLENITRELDIDLI
EGVTRVRLDPEMLEDKGLSVEVIIKKLKELDVGEVSRGDDPYEVIISLREEYLDYSKLEKLRSKLLGLHLKGIKEIRKVI
IQKRGDEYVIISEGSNLEEIMRIEGVDWRRVYTNNIHEIERVLGIEAARQAIIKEIKEILDDQGLDVDIRHIMLLADMMT
WTGHVRQIGRMGVAGEKPSVLARATFEMTVQKLLESAATSEEDRLYGVTENIIIGQTIPVGTGMVQIYMTPSLLKTEAGN
KGDRNE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=361, Percent_Identity=36.01108033241, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI4505939, Length=441, Percent_Identity=31.2925170068027, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI103471997, Length=146, Percent_Identity=35.6164383561644, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25145495, Length=379, Percent_Identity=36.9393139841689, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI71987878, Length=415, Percent_Identity=32.7710843373494, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71998295, Length=99, Percent_Identity=45.4545454545455, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324690, Length=373, Percent_Identity=34.5844504021448, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6320061, Length=407, Percent_Identity=31.4496314496315, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324917, Length=109, Percent_Identity=44.0366972477064, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281360912, Length=381, Percent_Identity=35.1706036745407, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17530899, Length=410, Percent_Identity=31.7073170731707, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17647875, Length=157, Percent_Identity=32.484076433121, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 46491; Mature: 46491

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGLTIEEIQQLLEERLKENVSQQVYEEVRNKLLNIAGKIVITRETVERFIEHIIERYHS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
SLIEPGEAVGTIAAQSLGEPSTQMTLRVFHYAGVREYNVTLGLPRLIEIVDARKKPETPI
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCCCE
MEIYLVDEYRRSEEKAREIARKIESTYLENITRELDIDLIEGVTRVRLDPEMLEDKGLSV
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHCCCHHHHHCCCCHH
EVIIKKLKELDVGEVSRGDDPYEVIISLREEYLDYSKLEKLRSKLLGLHLKGIKEIRKVI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQKRGDEYVIISEGSNLEEIMRIEGVDWRRVYTNNIHEIERVLGIEAARQAIIKEIKEIL
HHCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DDQGLDVDIRHIMLLADMMTWTGHVRQIGRMGVAGEKPSVLARATFEMTVQKLLESAATS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EEDRLYGVTENIIIGQTIPVGTGMVQIYMTPSLLKTEAGNKGDRNE
CCCCEECCCCCEEECCCCCCCCCEEEEEECHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQGLTIEEIQQLLEERLKENVSQQVYEEVRNKLLNIAGKIVITRETVERFIEHIIERYHS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
SLIEPGEAVGTIAAQSLGEPSTQMTLRVFHYAGVREYNVTLGLPRLIEIVDARKKPETPI
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCCCE
MEIYLVDEYRRSEEKAREIARKIESTYLENITRELDIDLIEGVTRVRLDPEMLEDKGLSV
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHCCCHHHHHCCCCHH
EVIIKKLKELDVGEVSRGDDPYEVIISLREEYLDYSKLEKLRSKLLGLHLKGIKEIRKVI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQKRGDEYVIISEGSNLEEIMRIEGVDWRRVYTNNIHEIERVLGIEAARQAIIKEIKEIL
HHCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DDQGLDVDIRHIMLLADMMTWTGHVRQIGRMGVAGEKPSVLARATFEMTVQKLLESAATS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EEDRLYGVTENIIIGQTIPVGTGMVQIYMTPSLLKTEAGNKGDRNE
CCCCEECCCCCEEECCCCCCCCCEEEEEECHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]