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The map label for this gene is tuf [H]

Identifier: 218884679

GI number: 218884679

Start: 1268578

End: 1269900

Strand: Reverse

Name: tuf [H]

Synonym: DKAM_1368

Alternate gene names: 218884679

Gene position: 1269900-1268578 (Counterclockwise)

Preceding gene: 218884680

Following gene: 218884678

Centisome position: 93.02

GC content: 46.94

Gene sequence:

>1323_bases
ATGAACGTGAGCGCGCCTCAGAAACCACATTTAAACATAGTGATAATTGGGCACGTAGACCATGGAAAAAGCACTATGAC
AGGGCATATACTCTATCGTCTAGGCTACTTCGATGAAAAAACCGTGAAGATGATCGAGGAAGAAGCAAAGAAGATGGGTA
AGGAGAGCTTCAAGTTTGCCTGGTTACTTGATAGAATGAAGGAAGAGCGTGAACGCGGTGTCACTATTTCACTATCATAC
ATGAAGTTCGAGACCAAGAAATACTTCTTCACCATCATAGATGCACCCGGTCACCGAGACTTCGTTAAAAACATGATCAC
CGGCGCAAGCCAGGCCGATGCAGCCATCCTAGTTGTAAGCGCCAGAAAAGGAGAGTTCGAGGCTGGAATGAGTGCCGAGG
GCCAGACCCGTGAGCACGCCATACTAGCTAGGACCATGGGTATAAACCAGCTAATTGTGGCCATAAACAAGATGGATGCA
ACCGAGCCACCATACAGTGAGAAGAGGTACAACGAGATTAAGGAGATCCTGGGTAAATTCCTGAAGGGACTGGGCTACGA
TATATCCAAGATACCATTCATCCCGGTGTCAGCGTGGACAGGTGAAAACCTGATTGAACGTTCGCCCAACATGCCGTGGT
ATAATGGTCCAACACTAGTAGAGGCATTGGACTTATTAACGGTGCCGCCCAAGCCACTTGATAAACCACTTAGAATACCG
ATACAAGATGTATACAACGTCTCCGGTGTTGGTGTCGTACCTGTTGGAAGGGTTGAAACCGGTGTACTTAAAGTAGGCGA
TAAAGTAGTATTCATGCCGCCAGGACTAATCGGTGAAGTCAAGAGTATAGAGACTCATTACACTAAGATCGAGAAAGCAG
AGCCCGGCGACAACATCGGGTTCAATGTAAAGGGTGTAGAGAAGAAGGATATTAAGCGCGGCGATGTGGCTGGATCCCTT
GATGTTCCCCCAACAGTTGCCGATGAATTCACCGCGAGAATAATGATAATGTGGCATCCAACAGCAATAGCCGTGGGATA
CACGCCAGTAATACATGTCCACACAGCCAGCGTGGCATGCAGGATAACCGAGATAATTTCAAAGATCGACCCTAGGACAG
GTAAGGAGGTTGAGAAGAACCCACAGTTCCTGAAGCAGGGAGACATAGCTATAGTTAAATTCAAACCGATAAAGCCACTC
GTAGTAGAGAAATACGCTGACTTCCCAGCTCTTGGAAGATTCGCTATGAGAGACATGGGTAAGACAATCGGTATTGGACA
GGTACTCGAGATCAAGCCAGCACAAGTCAATATCAAGAAATAG

Upstream 100 bases:

>100_bases
TAAACAAGGACGGAGATAATTACTGGCAGGGAGAAATACTTTATAAACACCCTGAAAGTTATTAAACTAGTTGGACATGT
TAATGTAGATAAGAAAGGTG

Downstream 100 bases:

>100_bases
TTTTTTAAACCCGAATTCTACACATTACGTTTCCAAGTATGCTTACAGGTATAAGGTTGGTCACGATGTCCGCAACAAGG
ATGGTTAAAATAAAGATCTG

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MNVSAPQKPHLNIVIIGHVDHGKSTMTGHILYRLGYFDEKTVKMIEEEAKKMGKESFKFAWLLDRMKEERERGVTISLSY
MKFETKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVSARKGEFEAGMSAEGQTREHAILARTMGINQLIVAINKMDA
TEPPYSEKRYNEIKEILGKFLKGLGYDISKIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDLLTVPPKPLDKPLRIP
IQDVYNVSGVGVVPVGRVETGVLKVGDKVVFMPPGLIGEVKSIETHYTKIEKAEPGDNIGFNVKGVEKKDIKRGDVAGSL
DVPPTVADEFTARIMIMWHPTAIAVGYTPVIHVHTASVACRITEIISKIDPRTGKEVEKNPQFLKQGDIAIVKFKPIKPL
VVEKYADFPALGRFAMRDMGKTIGIGQVLEIKPAQVNIKK

Sequences:

>Translated_440_residues
MNVSAPQKPHLNIVIIGHVDHGKSTMTGHILYRLGYFDEKTVKMIEEEAKKMGKESFKFAWLLDRMKEERERGVTISLSY
MKFETKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVSARKGEFEAGMSAEGQTREHAILARTMGINQLIVAINKMDA
TEPPYSEKRYNEIKEILGKFLKGLGYDISKIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDLLTVPPKPLDKPLRIP
IQDVYNVSGVGVVPVGRVETGVLKVGDKVVFMPPGLIGEVKSIETHYTKIEKAEPGDNIGFNVKGVEKKDIKRGDVAGSL
DVPPTVADEFTARIMIMWHPTAIAVGYTPVIHVHTASVACRITEIISKIDPRTGKEVEKNPQFLKQGDIAIVKFKPIKPL
VVEKYADFPALGRFAMRDMGKTIGIGQVLEIKPAQVNIKK
>Mature_440_residues
MNVSAPQKPHLNIVIIGHVDHGKSTMTGHILYRLGYFDEKTVKMIEEEAKKMGKESFKFAWLLDRMKEERERGVTISLSY
MKFETKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVSARKGEFEAGMSAEGQTREHAILARTMGINQLIVAINKMDA
TEPPYSEKRYNEIKEILGKFLKGLGYDISKIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDLLTVPPKPLDKPLRIP
IQDVYNVSGVGVVPVGRVETGVLKVGDKVVFMPPGLIGEVKSIETHYTKIEKAEPGDNIGFNVKGVEKKDIKRGDVAGSL
DVPPTVADEFTARIMIMWHPTAIAVGYTPVIHVHTASVACRITEIISKIDPRTGKEVEKNPQFLKQGDIAIVKFKPIKPL
VVEKYADFPALGRFAMRDMGKTIGIGQVLEIKPAQVNIKK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503471, Length=433, Percent_Identity=55.6581986143187, Blast_Score=521, Evalue=1e-148,
Organism=Homo sapiens, GI4503475, Length=439, Percent_Identity=54.4419134396355, Blast_Score=520, Evalue=1e-147,
Organism=Homo sapiens, GI223555963, Length=428, Percent_Identity=43.2242990654206, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI5729864, Length=428, Percent_Identity=43.2242990654206, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI194097354, Length=432, Percent_Identity=42.5925925925926, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI194018520, Length=432, Percent_Identity=42.5925925925926, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI194018522, Length=432, Percent_Identity=42.5925925925926, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI46094014, Length=432, Percent_Identity=42.1296296296296, Blast_Score=348, Evalue=8e-96,
Organism=Homo sapiens, GI34147630, Length=447, Percent_Identity=33.1096196868009, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI169162126, Length=140, Percent_Identity=40, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI239752724, Length=140, Percent_Identity=40, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI169162824, Length=140, Percent_Identity=40, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI45593140, Length=472, Percent_Identity=23.5169491525424, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=22.8985507246377, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI82546879, Length=418, Percent_Identity=25.1196172248804, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI157426893, Length=265, Percent_Identity=29.4339622641509, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789108, Length=438, Percent_Identity=32.4200913242009, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1790412, Length=438, Percent_Identity=29.9086757990868, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789737, Length=438, Percent_Identity=29.9086757990868, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI2367247, Length=385, Percent_Identity=25.974025974026, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788922, Length=316, Percent_Identity=27.8481012658228, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994988, Length=264, Percent_Identity=27.6515151515151, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17552884, Length=439, Percent_Identity=53.7585421412301, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17569207, Length=439, Percent_Identity=53.7585421412301, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI32566303, Length=439, Percent_Identity=48.74715261959, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32566629, Length=429, Percent_Identity=38.9277389277389, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI115532067, Length=426, Percent_Identity=39.906103286385, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI115532065, Length=426, Percent_Identity=39.6713615023474, Blast_Score=301, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI32566301, Length=171, Percent_Identity=57.3099415204678, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17556456, Length=445, Percent_Identity=31.2359550561798, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI25141371, Length=318, Percent_Identity=33.9622641509434, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI32566299, Length=63, Percent_Identity=55.5555555555556, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565438, Length=321, Percent_Identity=24.6105919003115, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=258, Percent_Identity=27.5193798449612, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17509919, Length=337, Percent_Identity=24.0356083086053, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6325337, Length=438, Percent_Identity=51.5981735159817, Blast_Score=494, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6319594, Length=438, Percent_Identity=51.5981735159817, Blast_Score=494, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320377, Length=429, Percent_Identity=39.6270396270396, Blast_Score=310, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6322937, Length=451, Percent_Identity=33.4811529933481, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324761, Length=437, Percent_Identity=32.9519450800915, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323320, Length=320, Percent_Identity=27.8125, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=152, Percent_Identity=33.5526315789474, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652838, Length=433, Percent_Identity=55.4272517321016, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI17137572, Length=433, Percent_Identity=55.4272517321016, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45553807, Length=433, Percent_Identity=54.7344110854504, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI45553816, Length=433, Percent_Identity=54.7344110854504, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24651721, Length=433, Percent_Identity=54.7344110854504, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI17864154, Length=433, Percent_Identity=54.7344110854504, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI45550900, Length=434, Percent_Identity=39.1705069124424, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI17137380, Length=428, Percent_Identity=38.3177570093458, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI281363316, Length=432, Percent_Identity=32.6388888888889, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI17864358, Length=432, Percent_Identity=32.6388888888889, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI19921738, Length=312, Percent_Identity=30.7692307692308, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922690, Length=315, Percent_Identity=26.031746031746, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19922512, Length=452, Percent_Identity=23.8938053097345, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24654852, Length=452, Percent_Identity=23.8938053097345, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647048, Length=366, Percent_Identity=23.224043715847, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24647046, Length=366, Percent_Identity=23.224043715847, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 48890; Mature: 48890

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSAPQKPHLNIVIIGHVDHGKSTMTGHILYRLGYFDEKTVKMIEEEAKKMGKESFKFA
CCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCHHHHHHH
WLLDRMKEERERGVTISLSYMKFETKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVS
HHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEE
ARKGEFEAGMSAEGQTREHAILARTMGINQLIVAINKMDATEPPYSEKRYNEIKEILGKF
ECCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LKGLGYDISKIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDLLTVPPKPLDKPLRIP
HHHCCCCCHHCCEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
IQDVYNVSGVGVVPVGRVETGVLKVGDKVVFMPPGLIGEVKSIETHYTKIEKAEPGDNIG
HHHHCCCCCCCEEECCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHEECCCCCCCCC
FNVKGVEKKDIKRGDVAGSLDVPPTVADEFTARIMIMWHPTAIAVGYTPVIHVHTASVAC
EEECCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEEEECCCEEEEEEHHHHHH
RITEIISKIDPRTGKEVEKNPQFLKQGDIAIVKFKPIKPLVVEKYADFPALGRFAMRDMG
HHHHHHHHCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHCC
KTIGIGQVLEIKPAQVNIKK
CCCCCCEEEEECCCEEEECC
>Mature Secondary Structure
MNVSAPQKPHLNIVIIGHVDHGKSTMTGHILYRLGYFDEKTVKMIEEEAKKMGKESFKFA
CCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCHHHHHHH
WLLDRMKEERERGVTISLSYMKFETKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVS
HHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEE
ARKGEFEAGMSAEGQTREHAILARTMGINQLIVAINKMDATEPPYSEKRYNEIKEILGKF
ECCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LKGLGYDISKIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDLLTVPPKPLDKPLRIP
HHHCCCCCHHCCEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
IQDVYNVSGVGVVPVGRVETGVLKVGDKVVFMPPGLIGEVKSIETHYTKIEKAEPGDNIG
HHHHCCCCCCCEEECCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHEECCCCCCCCC
FNVKGVEKKDIKRGDVAGSLDVPPTVADEFTARIMIMWHPTAIAVGYTPVIHVHTASVAC
EEECCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEEEECCCEEEEEEHHHHHH
RITEIISKIDPRTGKEVEKNPQFLKQGDIAIVKFKPIKPLVVEKYADFPALGRFAMRDMG
HHHHHHHHCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHCC
KTIGIGQVLEIKPAQVNIKK
CCCCCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7898436 [H]