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The map label for this gene is rtcA

Identifier: 218884596

GI number: 218884596

Start: 1202644

End: 1203717

Strand: Reverse

Name: rtcA

Synonym: DKAM_1285

Alternate gene names: 218884596

Gene position: 1203717-1202644 (Counterclockwise)

Preceding gene: 218884597

Following gene: 218884594

Centisome position: 88.17

GC content: 46.0

Gene sequence:

>1074_bases
ATGAGCATTCTAGAAATTGACGGCAGTATTGGAGAAGGCGGTGGACAAATACTTAGGTATGCTCTTGCATTATCCGCTTT
AACACTTAAGCCTATCAGGGTATATAATATAAGAGCGAAAAGAGACAACCCAGGCCTCCGCCCCCAGCACTTAACAGCTG
TTGAAGTACTTAAAGAGGTAACAGGGGCTGAAGTAGAGAACGCGAAAGTAGGCAGCATGGAGATCCTGTTTAAACCAACC
AGTAGGAGAAGCGGTGAGATGGAGATAGATATTGGAACCGCTGGAAGCATTTCACTAGTACTGCAAGCCATGCTTCCAGT
ATTATTGTTTGCCGAAGGAGACTCAAGGGCGAGGTTAAAGGGGGGAACAAATGTACCGTGGAGCCCTCCAATAGATTATA
TTAAACACGTCTTCCTATACAATATAAGCCACATGGGCGTTAGAGCCAATATCGAAGTGGTGAGGAGGGGACACTATCCT
AGGGGCGGTGGGTTAGTTAATGTGGAAGTTAAACGCGTCGAGGAGGCCTTGAAGCCATTAATGATTGTTAGAAGGGGGCG
TATAAGGGGTTTCAGAATACACAGCCACTGCGTTAAGCTACCTGCCCATGTAGCTGTGAGACAGTGTGAGTCGGCTAGAC
GTATTCTCTCAGGGATATTCAAGGAGAAAATAACAGAGGAGATAGAGACTTATCCACCAGATAAAGACCCTCATCTAGGT
CCTGGAAGCGGCATCCTTATCTACGTTGAAGCCGAGCCAGGGATAAGACTAGGCAGTGATTCATTAGGCGAGAAAGGAAA
ACCAGCTGAGAGAGTAGGTGAGGAAGCCGCTCTAATACTCATTGAGGAATTAGAGACAGGCATGGCTTTCGATAGGCATA
TGGGCGATATGCTGATACCCTATCTGTTTCTAGCCAAGGGGACAAGTAGAATAGGTGTTTCAATGATCACGCTTCACTTG
TTAACAGCCATAGAGGTTGGAAAGTTATTCTTTCCAGAGGCAAAGGTAGAAGTAGATGGAGAGCTGGGTAAACCAGGTAT
AATAACTATCCAGGGCGTTGGGTATAAGCCATAA

Upstream 100 bases:

>100_bases
CTCGAGGGCAATGAATACTGGAGGAAACTAGTGCCAAACGCTATCTCGAGATACATAGACGAAGTAAACGGAGTCGAGAG
AATAAGGGAGGTGTGTAGGA

Downstream 100 bases:

>100_bases
ACGCCTCTACTAATACCTTTCTTAAAGCATCCATGTTTATTTCCAGCAGTGCTGAGATACAAATGGGTTTCAAGCCGGTT
CTTGACTCGATTAATTCAAC

Product: putative RNA 3'-terminal phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSILEIDGSIGEGGGQILRYALALSALTLKPIRVYNIRAKRDNPGLRPQHLTAVEVLKEVTGAEVENAKVGSMEILFKPT
SRRSGEMEIDIGTAGSISLVLQAMLPVLLFAEGDSRARLKGGTNVPWSPPIDYIKHVFLYNISHMGVRANIEVVRRGHYP
RGGGLVNVEVKRVEEALKPLMIVRRGRIRGFRIHSHCVKLPAHVAVRQCESARRILSGIFKEKITEEIETYPPDKDPHLG
PGSGILIYVEAEPGIRLGSDSLGEKGKPAERVGEEAALILIEELETGMAFDRHMGDMLIPYLFLAKGTSRIGVSMITLHL
LTAIEVGKLFFPEAKVEVDGELGKPGIITIQGVGYKP

Sequences:

>Translated_357_residues
MSILEIDGSIGEGGGQILRYALALSALTLKPIRVYNIRAKRDNPGLRPQHLTAVEVLKEVTGAEVENAKVGSMEILFKPT
SRRSGEMEIDIGTAGSISLVLQAMLPVLLFAEGDSRARLKGGTNVPWSPPIDYIKHVFLYNISHMGVRANIEVVRRGHYP
RGGGLVNVEVKRVEEALKPLMIVRRGRIRGFRIHSHCVKLPAHVAVRQCESARRILSGIFKEKITEEIETYPPDKDPHLG
PGSGILIYVEAEPGIRLGSDSLGEKGKPAERVGEEAALILIEELETGMAFDRHMGDMLIPYLFLAKGTSRIGVSMITLHL
LTAIEVGKLFFPEAKVEVDGELGKPGIITIQGVGYKP
>Mature_356_residues
SILEIDGSIGEGGGQILRYALALSALTLKPIRVYNIRAKRDNPGLRPQHLTAVEVLKEVTGAEVENAKVGSMEILFKPTS
RRSGEMEIDIGTAGSISLVLQAMLPVLLFAEGDSRARLKGGTNVPWSPPIDYIKHVFLYNISHMGVRANIEVVRRGHYPR
GGGLVNVEVKRVEEALKPLMIVRRGRIRGFRIHSHCVKLPAHVAVRQCESARRILSGIFKEKITEEIETYPPDKDPHLGP
GSGILIYVEAEPGIRLGSDSLGEKGKPAERVGEEAALILIEELETGMAFDRHMGDMLIPYLFLAKGTSRIGVSMITLHLL
TAIEVGKLFFPEAKVEVDGELGKPGIITIQGVGYKP

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=359, Percent_Identity=38.4401114206128, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI195927044, Length=372, Percent_Identity=37.0967741935484, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI157426877, Length=295, Percent_Identity=28.135593220339, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI48994937, Length=340, Percent_Identity=37.9411764705882, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI32564710, Length=331, Percent_Identity=27.4924471299094, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324564, Length=350, Percent_Identity=22.8571428571429, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI18543269, Length=354, Percent_Identity=35.5932203389831, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI18860065, Length=329, Percent_Identity=24.0121580547112, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_DESK1 (B8D680)

Other databases:

- EMBL:   CP001140
- RefSeq:   YP_002428978.1
- GeneID:   7171844
- GenomeReviews:   CP001140_GR
- KEGG:   dka:DKAM_1285
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- ProtClustDB:   CLSK2399617
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR013791
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF52913 RNA3'-term_phos_cycl_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 39042; Mature: 38911

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 319-319 BINDING 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILEIDGSIGEGGGQILRYALALSALTLKPIRVYNIRAKRDNPGLRPQHLTAVEVLKEV
CEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHH
TGAEVENAKVGSMEILFKPTSRRSGEMEIDIGTAGSISLVLQAMLPVLLFAEGDSRARLK
HCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEECCCCCEEEC
GGTNVPWSPPIDYIKHVFLYNISHMGVRANIEVVRRGHYPRGGGLVNVEVKRVEEALKPL
CCCCCCCCCCHHHHHHHHHHHHHCCCEEECEEEEECCCCCCCCCEEEEEHHHHHHHHHHH
MIVRRGRIRGFRIHSHCVKLPAHVAVRQCESARRILSGIFKEKITEEIETYPPDKDPHLG
HHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PGSGILIYVEAEPGIRLGSDSLGEKGKPAERVGEEAALILIEELETGMAFDRHMGDMLIP
CCCCEEEEEECCCCCEECCCCCCCCCCCHHHHCCCEEEEEEEHHHCCHHHHHHHHHHHHH
YLFLAKGTSRIGVSMITLHLLTAIEVGKLFFPEAKVEVDGELGKPGIITIQGVGYKP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
SILEIDGSIGEGGGQILRYALALSALTLKPIRVYNIRAKRDNPGLRPQHLTAVEVLKEV
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHH
TGAEVENAKVGSMEILFKPTSRRSGEMEIDIGTAGSISLVLQAMLPVLLFAEGDSRARLK
HCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEECCCCCEEEC
GGTNVPWSPPIDYIKHVFLYNISHMGVRANIEVVRRGHYPRGGGLVNVEVKRVEEALKPL
CCCCCCCCCCHHHHHHHHHHHHHCCCEEECEEEEECCCCCCCCCEEEEEHHHHHHHHHHH
MIVRRGRIRGFRIHSHCVKLPAHVAVRQCESARRILSGIFKEKITEEIETYPPDKDPHLG
HHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PGSGILIYVEAEPGIRLGSDSLGEKGKPAERVGEEAALILIEELETGMAFDRHMGDMLIP
CCCCEEEEEECCCCCEECCCCCCCCCCCHHHHCCCEEEEEEEHHHCCHHHHHHHHHHHHH
YLFLAKGTSRIGVSMITLHLLTAIEVGKLFFPEAKVEVDGELGKPGIITIQGVGYKP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA