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The map label for this gene is ppaX [H]

Identifier: 218884324

GI number: 218884324

Start: 963369

End: 964028

Strand: Reverse

Name: ppaX [H]

Synonym: DKAM_1013

Alternate gene names: 218884324

Gene position: 964028-963369 (Counterclockwise)

Preceding gene: 218884325

Following gene: 218884323

Centisome position: 70.61

GC content: 49.85

Gene sequence:

>660_bases
ATGAAGATTCCAGTGAGTAGTCTTTTAAACAGTGTCAGACTAATCCTCTTCGACATGGATGGCACGCTTGTAGATAGCGA
GGACTTCATTGTCTGGAGCTTTACCGAGGCAGCACGTCTCACTGGTCTACGCGTCGATCCATCCATAATCAGGGAGTTGA
TAGGCTACCCCCTCGAATACCTCCTCAACGAGATACTGGGTGGGGTCAGTGAGGAGAAGGCGAGGGAGTTCATTGAGGTT
AGGAGGAGGCTTGTCTGGGAGAACTGGAGTAAACACGTTAAATTATTCCCGGACGTGGTGCCGGTACTAGAATACCTGTC
TTCAAAGGGTTATGTACTTGGAGTAGCTTCCTCCAGCATAGTGGAGAGAATAAATGCTTTCCTCAACCACCTCGGTGTAT
CAAGGTACTTTAACGTGGTTTCTGGAGTGACACCAGGTGTTAGGGGAAAGCCTGAGCCAGACGTGATATTAAATGCGTTG
AGGATGACTGGTATGCATCCAGGCGAAACACTATATATCGGTGATAGAGTAGTCGACTGCATAGCTGCCAGGCAGGCCGG
GGTCGGGGTAGTGATTGTTAACAGAAGGGGAACAGGGCTACCCACCAGTGGATGCAGCCCGGATGCAGTGGTTCAGAGCC
TCATAGAGCTCATCGAATAA

Upstream 100 bases:

>100_bases
TCTGATGAGGAGTTACTCAAGTACTGTAATATTGTTGCAGAGTCTCCAAGCGGCTTTGGTTTAGCCGAGATTATTTCACG
TGTTTTAAACGGGATGGGTG

Downstream 100 bases:

>100_bases
AACAATTAATACCCTTATGAAACATAGAGATACATGGCCCGCGGCACTGCCCGGCGGCGGCATCGCTTTGACGCCGAGCA
TAGCCCGGTGAATGATAAGC

Product: HAD-superfamily hydrolase, subfamily IA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKIPVSSLLNSVRLILFDMDGTLVDSEDFIVWSFTEAARLTGLRVDPSIIRELIGYPLEYLLNEILGGVSEEKAREFIEV
RRRLVWENWSKHVKLFPDVVPVLEYLSSKGYVLGVASSSIVERINAFLNHLGVSRYFNVVSGVTPGVRGKPEPDVILNAL
RMTGMHPGETLYIGDRVVDCIAARQAGVGVVIVNRRGTGLPTSGCSPDAVVQSLIELIE

Sequences:

>Translated_219_residues
MKIPVSSLLNSVRLILFDMDGTLVDSEDFIVWSFTEAARLTGLRVDPSIIRELIGYPLEYLLNEILGGVSEEKAREFIEV
RRRLVWENWSKHVKLFPDVVPVLEYLSSKGYVLGVASSSIVERINAFLNHLGVSRYFNVVSGVTPGVRGKPEPDVILNAL
RMTGMHPGETLYIGDRVVDCIAARQAGVGVVIVNRRGTGLPTSGCSPDAVVQSLIELIE
>Mature_219_residues
MKIPVSSLLNSVRLILFDMDGTLVDSEDFIVWSFTEAARLTGLRVDPSIIRELIGYPLEYLLNEILGGVSEEKAREFIEV
RRRLVWENWSKHVKLFPDVVPVLEYLSSKGYVLGVASSSIVERINAFLNHLGVSRYFNVVSGVTPGVRGKPEPDVILNAL
RMTGMHPGETLYIGDRVVDCIAARQAGVGVVIVNRRGTGLPTSGCSPDAVVQSLIELIE

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=240, Percent_Identity=27.9166666666667, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 24136; Mature: 24136

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPVSSLLNSVRLILFDMDGTLVDSEDFIVWSFTEAARLTGLRVDPSIIRELIGYPLEY
CCCCHHHHHHHHEEEEECCCCCEECCCCEEEEEEHHHHHHCCCCCCHHHHHHHHCCCHHH
LLNEILGGVSEEKAREFIEVRRRLVWENWSKHVKLFPDVVPVLEYLSSKGYVLGVASSSI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHH
VERINAFLNHLGVSRYFNVVSGVTPGVRGKPEPDVILNALRMTGMHPGETLYIGDRVVDC
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHH
IAARQAGVGVVIVNRRGTGLPTSGCSPDAVVQSLIELIE
HHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MKIPVSSLLNSVRLILFDMDGTLVDSEDFIVWSFTEAARLTGLRVDPSIIRELIGYPLEY
CCCCHHHHHHHHEEEEECCCCCEECCCCEEEEEEHHHHHHCCCCCCHHHHHHHHCCCHHH
LLNEILGGVSEEKAREFIEVRRRLVWENWSKHVKLFPDVVPVLEYLSSKGYVLGVASSSI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHH
VERINAFLNHLGVSRYFNVVSGVTPGVRGKPEPDVILNALRMTGMHPGETLYIGDRVVDC
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHH
IAARQAGVGVVIVNRRGTGLPTSGCSPDAVVQSLIELIE
HHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]