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The map label for this gene is pmbA [C]

Identifier: 218884245

GI number: 218884245

Start: 887601

End: 888926

Strand: Direct

Name: pmbA [C]

Synonym: DKAM_0934

Alternate gene names: 218884245

Gene position: 887601-888926 (Clockwise)

Preceding gene: 218884244

Following gene: 218884251

Centisome position: 65.02

GC content: 44.12

Gene sequence:

>1326_bases
ATGGAGATATATAAGAGGATATTAAAGGAGGCCCTTGATAAGGGGTTCCAGGAGGCAATCGTTAGAGTGCTTGAATCCGA
GAGAACGATGACCAAGATAGCTAACAGCGAGCCAAGCGTGGTGCAACACTGGAGGAGCCTCGATATAGGTTTATACCTTG
TAAAAGATAAGAGGATATTTATAGCTGAGGTAAGAGGCTTAGATCCAAGTGGGACAGAATACACCTTGAACCAATTATTC
TCGATAGCCGACAAGCTCAGGGAATCCGAGCTCTACGCCCCTCTCCCAGAACCCGGTAAACCAGTTGAAGTAGGTAACCT
AGTTGATAAGAATATCGATAAGTACATTGAGGATCCATCGAGTTTAGCTGAGGAAGTAATTAGCACAGCACATGAGGAGA
GAATAGATTATATAGCTGGAATGATCGACCTAAAGCATGAGAAAGAATATATAGCGTCGTCAAAAGGATTCGAGGGGATG
CATGAATATACATCAATGGAGTCATATATACGGGCATTCGCCGGGGAAGAAGGTAGTGGTCAATGGAGCACCTGCTCCAC
AAAGCTTAGGAGAGATAAACTAGCCGAGATGGCCAGGATAGCTGCGAGGTATGCTGTCGACTCCAAGAGTAGGGTAGACA
TAGAGCCCGGGATCTATGATTTAATACTTTCCCCAATGGTGTTCGGGAACCTATTAGACTACATATCATACATGGCTTCA
GGCTTCAGCATCCTCACCGGGATGTCCATGTTTATAAAGAACAAGCCAGGTGATAAGGTAGCCTCTGAGAAATTCACTCT
TATAGATAACCCACACGAACCCGAGTTACCGGGTAGCAGGGGCTTCGACCTAGAGGGAGTACCCACTATAAGGAAGCCCA
TCATAGAGAAAGGGATACTGGCAACAATACTCCACAACTCTAAAACAGCCGCGAAATTCAGCGCATCAACCACCGGTAAC
GCTGGATGGATCTCACCAGAGCCGTGGAATCTAATAGTGGAACCAGGAGATTACAGGCTCGAGGAGTTAATTAGTGAGGT
TAAGAAAGGGATATTGATCAACAATAACTGGTATACTAGGTTCCAGAACTATGTTGAGGGAGAATTCTCAACCATAACGA
GGGATGCATTATTCTTTATCGAGAACGGCGAGATAAAGACTGCTGTGAGAAAAATAAGGATAGCTGATAAACTCGGGAAC
ATATTAAGAAATATTGATGGGCTAACAAAGGAGAGATACGATGTGAAATGGTGGGAGATAAGCTATCCAACCAGGGCGCC
ATATATATTAGTAAGAGGAGTAAATACTTCAAAACACACTATTTAG

Upstream 100 bases:

>100_bases
GCGGTAAAGGCGAACCCTCCCAGGGCGTACCCGTATGGTTTGGAGGCCCGAATGTGAAGCTTAAGAATATAAGGCTTAGA
AGTACTGTGGGGTGATTTGA

Downstream 100 bases:

>100_bases
TTTGAGTAGTCTTTAATATGGCGTTGCTTCATTGAATCATGATTAATTTTTAACTGGCTACCACTTTTCCAGCTATTCTA
TTCTTAATATACTCATCAAA

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MEIYKRILKEALDKGFQEAIVRVLESERTMTKIANSEPSVVQHWRSLDIGLYLVKDKRIFIAEVRGLDPSGTEYTLNQLF
SIADKLRESELYAPLPEPGKPVEVGNLVDKNIDKYIEDPSSLAEEVISTAHEERIDYIAGMIDLKHEKEYIASSKGFEGM
HEYTSMESYIRAFAGEEGSGQWSTCSTKLRRDKLAEMARIAARYAVDSKSRVDIEPGIYDLILSPMVFGNLLDYISYMAS
GFSILTGMSMFIKNKPGDKVASEKFTLIDNPHEPELPGSRGFDLEGVPTIRKPIIEKGILATILHNSKTAAKFSASTTGN
AGWISPEPWNLIVEPGDYRLEELISEVKKGILINNNWYTRFQNYVEGEFSTITRDALFFIENGEIKTAVRKIRIADKLGN
ILRNIDGLTKERYDVKWWEISYPTRAPYILVRGVNTSKHTI

Sequences:

>Translated_441_residues
MEIYKRILKEALDKGFQEAIVRVLESERTMTKIANSEPSVVQHWRSLDIGLYLVKDKRIFIAEVRGLDPSGTEYTLNQLF
SIADKLRESELYAPLPEPGKPVEVGNLVDKNIDKYIEDPSSLAEEVISTAHEERIDYIAGMIDLKHEKEYIASSKGFEGM
HEYTSMESYIRAFAGEEGSGQWSTCSTKLRRDKLAEMARIAARYAVDSKSRVDIEPGIYDLILSPMVFGNLLDYISYMAS
GFSILTGMSMFIKNKPGDKVASEKFTLIDNPHEPELPGSRGFDLEGVPTIRKPIIEKGILATILHNSKTAAKFSASTTGN
AGWISPEPWNLIVEPGDYRLEELISEVKKGILINNNWYTRFQNYVEGEFSTITRDALFFIENGEIKTAVRKIRIADKLGN
ILRNIDGLTKERYDVKWWEISYPTRAPYILVRGVNTSKHTI
>Mature_441_residues
MEIYKRILKEALDKGFQEAIVRVLESERTMTKIANSEPSVVQHWRSLDIGLYLVKDKRIFIAEVRGLDPSGTEYTLNQLF
SIADKLRESELYAPLPEPGKPVEVGNLVDKNIDKYIEDPSSLAEEVISTAHEERIDYIAGMIDLKHEKEYIASSKGFEGM
HEYTSMESYIRAFAGEEGSGQWSTCSTKLRRDKLAEMARIAARYAVDSKSRVDIEPGIYDLILSPMVFGNLLDYISYMAS
GFSILTGMSMFIKNKPGDKVASEKFTLIDNPHEPELPGSRGFDLEGVPTIRKPIIEKGILATILHNSKTAAKFSASTTGN
AGWISPEPWNLIVEPGDYRLEELISEVKKGILINNNWYTRFQNYVEGEFSTITRDALFFIENGEIKTAVRKIRIADKLGN
ILRNIDGLTKERYDVKWWEISYPTRAPYILVRGVNTSKHTI

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=187, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49940; Mature: 49940

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIYKRILKEALDKGFQEAIVRVLESERTMTKIANSEPSVVQHWRSLDIGLYLVKDKRIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCEEE
IAEVRGLDPSGTEYTLNQLFSIADKLRESELYAPLPEPGKPVEVGNLVDKNIDKYIEDPS
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHH
SLAEEVISTAHEERIDYIAGMIDLKHEKEYIASSKGFEGMHEYTSMESYIRAFAGEEGSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
QWSTCSTKLRRDKLAEMARIAARYAVDSKSRVDIEPGIYDLILSPMVFGNLLDYISYMAS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
GFSILTGMSMFIKNKPGDKVASEKFTLIDNPHEPELPGSRGFDLEGVPTIRKPIIEKGIL
HHHHHHHHHHHHCCCCCCCHHCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ATILHNSKTAAKFSASTTGNAGWISPEPWNLIVEPGDYRLEELISEVKKGILINNNWYTR
HHHHHCCCHHHEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCEEECCCHHHH
FQNYVEGEFSTITRDALFFIENGEIKTAVRKIRIADKLGNILRNIDGLTKERYDVKWWEI
HHHHHCCHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SYPTRAPYILVRGVNTSKHTI
CCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MEIYKRILKEALDKGFQEAIVRVLESERTMTKIANSEPSVVQHWRSLDIGLYLVKDKRIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCEEE
IAEVRGLDPSGTEYTLNQLFSIADKLRESELYAPLPEPGKPVEVGNLVDKNIDKYIEDPS
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHH
SLAEEVISTAHEERIDYIAGMIDLKHEKEYIASSKGFEGMHEYTSMESYIRAFAGEEGSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
QWSTCSTKLRRDKLAEMARIAARYAVDSKSRVDIEPGIYDLILSPMVFGNLLDYISYMAS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
GFSILTGMSMFIKNKPGDKVASEKFTLIDNPHEPELPGSRGFDLEGVPTIRKPIIEKGIL
HHHHHHHHHHHHCCCCCCCHHCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ATILHNSKTAAKFSASTTGNAGWISPEPWNLIVEPGDYRLEELISEVKKGILINNNWYTR
HHHHHCCCHHHEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCEEECCCHHHH
FQNYVEGEFSTITRDALFFIENGEIKTAVRKIRIADKLGNILRNIDGLTKERYDVKWWEI
HHHHHCCHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SYPTRAPYILVRGVNTSKHTI
CCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]