Click here to switch to the map view.

The map label for this gene is pycB [H]

Identifier: 218883778

GI number: 218883778

Start: 435199

End: 436608

Strand: Reverse

Name: pycB [H]

Synonym: DKAM_0467

Alternate gene names: 218883778

Gene position: 436608-435199 (Counterclockwise)

Preceding gene: 218883779

Following gene: 218883777

Centisome position: 31.98

GC content: 47.52

Gene sequence:

>1410_bases
ATGTTTCAATGGGTGATATTAGTGGTTGAAATAGTTGATACAACACTGAGAGATGCTCACCAATCATTGATTGCAACCCG
TTTAAGAACCGAGGACATGATCCCCATTCTCGAGAAGATCGATAAGGCAGGTTTCTACAGTATCGAGATGTGGGGTGGAG
CTACATTCGACGTCATGATAAGGTATTTGAATGAAGATCCCTGGGAGAGATTACGGCTTATACGTGAAAGAGTCCACAGG
ACCAAGCTTCAAATGCTTTTACGTGGACAGAACCTGGTTGGATACAGGCATTACCCGGATGACGTCGTGGAAAAGTTTGT
CGAGCTCTCCTACAAGAATGGCATAGATGTATTCAGGATCTTTGATGCATTAAATGATGTAAGGAATATGAGGACATCCA
TAGCTAAAGCCAAGAAGCTTGGCGCTATAGTCCAGGGCACGATAACGTATACTATAAGCCCTGTCCACACCATAGAGCAC
TACCTGAAGATAGCCGAGGAGCTCGCTGCGTTGGAGGTAGACCATATAACGATAAAGGATATGTCAGGTATACTAGACCC
ATATACAGCATATACGCTGGTTAAAGCGTTAAAGGAGAGGTTAAAGATACCTGTTGACATTCACTCACATTTCACAGGTG
GGCTAGCTGTAGCCACATATGTTAAAGCTGTTGAAGCCGGAGCAGACTTCATCGATACATCGATAAGTCCCCTAGCATTC
GGCTCAGGTCAACCAGGTATACAGACAGTCTATCACGCGCTCCCTCCTGAGAATAGGCCAAAGATAGATCTAAAGGTGGT
GGGGGAGATAAGCAGTTACCTGGAGAAGATTGTTTTCACCAAGTACAAGGACCTAATAAACATAAAGGTCTTCATCCCGG
ACCCCAATGTCCTTGAGCACCAGGTGCCAGGCGGTATGATAACCAATTTCATCATGCAGCTTAAGCAGCTGGGGGCAGAG
GATAAGCTGGAAGAGGTATTAGCGGAGGTGAAGCGCGTCAGGGAAGACCTGGGATGGCCTCCACTAGTCACCCCCTCCTC
ACAGATTGTGGGGGCCCAGGCTGTTCTCAACGTTATAAACGGCCGTTACAAGGTTGTGACTAAGGAGGTCAGGGACTATG
TGAAGGGGCTGTATGGTAGGCCGCCTGCACCAATAAAAGAGGAGATTATAAGGCTGGTTCTAGGTAATGAGAAACCTATT
GAGGTGCGCCCGGCCGACCTATTGAAGCCCATGTATGAGGAATGCTATAAAACCGTTAAGGAGAAGGGGTATTATAGTAA
GGAGGAGGACGTACTCACCTACTGCCTATTCCCCGAGGTTGCTGCGGAGTTCTTTGAGAAGACTAGGAAGCGGGCTGTAG
CGGTGAAGGTTGCAAGCCGGGATCTATGGGATGAGATACTGGGCTTCTGA

Upstream 100 bases:

>100_bases
TAGGGGCTTCCTTCTCGGGTACGCTAGTCTTTATAATACAGATATTAAATATATCGATAATAATTAACTTGGATTAGAAT
AGAATAAGGCTTTCCCCCAC

Downstream 100 bases:

>100_bases
GCCAGTAGGGTGTATTGTCATTGAACACCAGGTATATTCTAAACCTGCGCCACATACCCAGAACCGGCTGGCTTCTGAGA
GGTGTACCCCCCGTTATCGC

Product: Pyruvate/oxaloacetate carboxyltransferase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MFQWVILVVEIVDTTLRDAHQSLIATRLRTEDMIPILEKIDKAGFYSIEMWGGATFDVMIRYLNEDPWERLRLIRERVHR
TKLQMLLRGQNLVGYRHYPDDVVEKFVELSYKNGIDVFRIFDALNDVRNMRTSIAKAKKLGAIVQGTITYTISPVHTIEH
YLKIAEELAALEVDHITIKDMSGILDPYTAYTLVKALKERLKIPVDIHSHFTGGLAVATYVKAVEAGADFIDTSISPLAF
GSGQPGIQTVYHALPPENRPKIDLKVVGEISSYLEKIVFTKYKDLINIKVFIPDPNVLEHQVPGGMITNFIMQLKQLGAE
DKLEEVLAEVKRVREDLGWPPLVTPSSQIVGAQAVLNVINGRYKVVTKEVRDYVKGLYGRPPAPIKEEIIRLVLGNEKPI
EVRPADLLKPMYEECYKTVKEKGYYSKEEDVLTYCLFPEVAAEFFEKTRKRAVAVKVASRDLWDEILGF

Sequences:

>Translated_469_residues
MFQWVILVVEIVDTTLRDAHQSLIATRLRTEDMIPILEKIDKAGFYSIEMWGGATFDVMIRYLNEDPWERLRLIRERVHR
TKLQMLLRGQNLVGYRHYPDDVVEKFVELSYKNGIDVFRIFDALNDVRNMRTSIAKAKKLGAIVQGTITYTISPVHTIEH
YLKIAEELAALEVDHITIKDMSGILDPYTAYTLVKALKERLKIPVDIHSHFTGGLAVATYVKAVEAGADFIDTSISPLAF
GSGQPGIQTVYHALPPENRPKIDLKVVGEISSYLEKIVFTKYKDLINIKVFIPDPNVLEHQVPGGMITNFIMQLKQLGAE
DKLEEVLAEVKRVREDLGWPPLVTPSSQIVGAQAVLNVINGRYKVVTKEVRDYVKGLYGRPPAPIKEEIIRLVLGNEKPI
EVRPADLLKPMYEECYKTVKEKGYYSKEEDVLTYCLFPEVAAEFFEKTRKRAVAVKVASRDLWDEILGF
>Mature_469_residues
MFQWVILVVEIVDTTLRDAHQSLIATRLRTEDMIPILEKIDKAGFYSIEMWGGATFDVMIRYLNEDPWERLRLIRERVHR
TKLQMLLRGQNLVGYRHYPDDVVEKFVELSYKNGIDVFRIFDALNDVRNMRTSIAKAKKLGAIVQGTITYTISPVHTIEH
YLKIAEELAALEVDHITIKDMSGILDPYTAYTLVKALKERLKIPVDIHSHFTGGLAVATYVKAVEAGADFIDTSISPLAF
GSGQPGIQTVYHALPPENRPKIDLKVVGEISSYLEKIVFTKYKDLINIKVFIPDPNVLEHQVPGGMITNFIMQLKQLGAE
DKLEEVLAEVKRVREDLGWPPLVTPSSQIVGAQAVLNVINGRYKVVTKEVRDYVKGLYGRPPAPIKEEIIRLVLGNEKPI
EVRPADLLKPMYEECYKTVKEKGYYSKEEDVLTYCLFPEVAAEFFEKTRKRAVAVKVASRDLWDEILGF

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=460, Percent_Identity=34.5652173913044, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI106049295, Length=460, Percent_Identity=34.5652173913044, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI106049292, Length=460, Percent_Identity=34.5652173913044, Blast_Score=252, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17562816, Length=458, Percent_Identity=36.0262008733624, Blast_Score=272, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6319695, Length=473, Percent_Identity=34.6723044397463, Blast_Score=273, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6321376, Length=473, Percent_Identity=34.6723044397463, Blast_Score=271, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24652212, Length=475, Percent_Identity=34.9473684210526, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24652210, Length=475, Percent_Identity=34.9473684210526, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24652214, Length=475, Percent_Identity=34.9473684210526, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI19921944, Length=475, Percent_Identity=34.9473684210526, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24652216, Length=475, Percent_Identity=34.9473684210526, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI281363050, Length=475, Percent_Identity=34.9473684210526, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24652224, Length=475, Percent_Identity=34.9473684210526, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24652222, Length=475, Percent_Identity=34.9473684210526, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24652220, Length=475, Percent_Identity=34.9473684210526, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24652218, Length=475, Percent_Identity=34.9473684210526, Blast_Score=262, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 53391; Mature: 53391

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQWVILVVEIVDTTLRDAHQSLIATRLRTEDMIPILEKIDKAGFYSIEMWGGATFDVMI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
RYLNEDPWERLRLIRERVHRTKLQMLLRGQNLVGYRHYPDDVVEKFVELSYKNGIDVFRI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
FDALNDVRNMRTSIAKAKKLGAIVQGTITYTISPVHTIEHYLKIAEELAALEVDHITIKD
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEHHH
MSGILDPYTAYTLVKALKERLKIPVDIHSHFTGGLAVATYVKAVEAGADFIDTSISPLAF
HHHCCCHHHHHHHHHHHHHHCCCCEEHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCC
GSGQPGIQTVYHALPPENRPKIDLKVVGEISSYLEKIVFTKYKDLINIKVFIPDPNVLEH
CCCCCHHHHHHHHCCCCCCCCEEHEEHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHC
QVPGGMITNFIMQLKQLGAEDKLEEVLAEVKRVREDLGWPPLVTPSSQIVGAQAVLNVIN
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
GRYKVVTKEVRDYVKGLYGRPPAPIKEEIIRLVLGNEKPIEVRPADLLKPMYEECYKTVK
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHH
EKGYYSKEEDVLTYCLFPEVAAEFFEKTRKRAVAVKVASRDLWDEILGF
HCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFQWVILVVEIVDTTLRDAHQSLIATRLRTEDMIPILEKIDKAGFYSIEMWGGATFDVMI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
RYLNEDPWERLRLIRERVHRTKLQMLLRGQNLVGYRHYPDDVVEKFVELSYKNGIDVFRI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
FDALNDVRNMRTSIAKAKKLGAIVQGTITYTISPVHTIEHYLKIAEELAALEVDHITIKD
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEHHH
MSGILDPYTAYTLVKALKERLKIPVDIHSHFTGGLAVATYVKAVEAGADFIDTSISPLAF
HHHCCCHHHHHHHHHHHHHHCCCCEEHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCC
GSGQPGIQTVYHALPPENRPKIDLKVVGEISSYLEKIVFTKYKDLINIKVFIPDPNVLEH
CCCCCHHHHHHHHCCCCCCCCEEHEEHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHC
QVPGGMITNFIMQLKQLGAEDKLEEVLAEVKRVREDLGWPPLVTPSSQIVGAQAVLNVIN
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
GRYKVVTKEVRDYVKGLYGRPPAPIKEEIIRLVLGNEKPIEVRPADLLKPMYEECYKTVK
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHH
EKGYYSKEEDVLTYCLFPEVAAEFFEKTRKRAVAVKVASRDLWDEILGF
HCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]