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The map label for this gene is hycG [H]

Identifier: 218883730

GI number: 218883730

Start: 390850

End: 391332

Strand: Reverse

Name: hycG [H]

Synonym: DKAM_0419

Alternate gene names: 218883730

Gene position: 391332-390850 (Counterclockwise)

Preceding gene: 218883731

Following gene: 218883729

Centisome position: 28.66

GC content: 49.48

Gene sequence:

>483_bases
TTGGATAATGAGGTTGTAGAGGGGAAAACTACTAGAAGAGAATACAAGGTAATCAGGTATAGCCCATGGCTAGTGCACTT
CAACACTGGTGCCTGTAATGGATGTGATATAGAGGTCTTAGCAGCAATCACCCCATTATACGACCCAGAGAGATTCGGCG
TGAAGCTCGCGCCTAGCATAAGGCATGGAGATATACTAGTGGTGACTGGGGCTGTAACCAAGAAAGCAGCTGAGAGACTT
AAGAGGCTATATGATCAAATGCCCTGTCCTAAATTCGTTATCGCTGTAGGGGCTTGTGCCTGTACTGGCGGTGTGTTTTC
CAAGTCGTATCCTGTCCTAGCCGGCGCTGATAAGGCTGTCCCGGTTTCAATGTATATCCCGGGATGCCCTCCACGCCCGG
AGGCCATTCTAGATGGTATTGTGAAGCTCCTTAAGAAGCTTGAGAGTGGCGAGAGCGGTGATGGCTGTGGAGGAAAAGCT
TAG

Upstream 100 bases:

>100_bases
TCGGGCAGGTGATCGTAGCTGGTTTAATTAGGTAAACCTACCGCTTGATTTATTTATCCTAGTTAATGAATCCTATAACC
ATGGCGAGGGTGCTTACGCC

Downstream 100 bases:

>100_bases
CTTCATGGATAAATATGTGATTGAGAAAGGCTTTGTTAAACCAAATAGAAGGCTCTTCACTATTAGATCAGAGCATGTGA
GACAAGTGTTCACAGAGTTT

Product: NADH-quinone oxidoreductase subunit B

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MDNEVVEGKTTRREYKVIRYSPWLVHFNTGACNGCDIEVLAAITPLYDPERFGVKLAPSIRHGDILVVTGAVTKKAAERL
KRLYDQMPCPKFVIAVGACACTGGVFSKSYPVLAGADKAVPVSMYIPGCPPRPEAILDGIVKLLKKLESGESGDGCGGKA

Sequences:

>Translated_160_residues
MDNEVVEGKTTRREYKVIRYSPWLVHFNTGACNGCDIEVLAAITPLYDPERFGVKLAPSIRHGDILVVTGAVTKKAAERL
KRLYDQMPCPKFVIAVGACACTGGVFSKSYPVLAGADKAVPVSMYIPGCPPRPEAILDGIVKLLKKLESGESGDGCGGKA
>Mature_160_residues
MDNEVVEGKTTRREYKVIRYSPWLVHFNTGACNGCDIEVLAAITPLYDPERFGVKLAPSIRHGDILVVTGAVTKKAAERL
KRLYDQMPCPKFVIAVGACACTGGVFSKSYPVLAGADKAVPVSMYIPGCPPRPEAILDGIVKLLKKLESGESGDGCGGKA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=130, Percent_Identity=44.6153846153846, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI1789074, Length=142, Percent_Identity=46.4788732394366, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1788834, Length=141, Percent_Identity=42.5531914893617, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1788624, Length=128, Percent_Identity=42.1875, Blast_Score=103, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17509685, Length=130, Percent_Identity=44.6153846153846, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24651058, Length=128, Percent_Identity=43.75, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI18859983, Length=128, Percent_Identity=42.1875, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24642371, Length=128, Percent_Identity=42.1875, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 17112; Mature: 17112

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNEVVEGKTTRREYKVIRYSPWLVHFNTGACNGCDIEVLAAITPLYDPERFGVKLAPSI
CCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCCHHHEEHHCCCCCCHHCCEEECCCC
RHGDILVVTGAVTKKAAERLKRLYDQMPCPKFVIAVGACACTGGVFSKSYPVLAGADKAV
CCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCC
PVSMYIPGCPPRPEAILDGIVKLLKKLESGESGDGCGGKA
EEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDNEVVEGKTTRREYKVIRYSPWLVHFNTGACNGCDIEVLAAITPLYDPERFGVKLAPSI
CCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCCHHHEEHHCCCCCCHHCCEEECCCC
RHGDILVVTGAVTKKAAERLKRLYDQMPCPKFVIAVGACACTGGVFSKSYPVLAGADKAV
CCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCC
PVSMYIPGCPPRPEAILDGIVKLLKKLESGESGDGCGGKA
EEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]