Click here to switch to the map view.

The map label for this gene is cooH [H]

Identifier: 218883728

GI number: 218883728

Start: 389201

End: 390418

Strand: Reverse

Name: cooH [H]

Synonym: DKAM_0417

Alternate gene names: 218883728

Gene position: 390418-389201 (Counterclockwise)

Preceding gene: 218883729

Following gene: 218883727

Centisome position: 28.6

GC content: 44.91

Gene sequence:

>1218_bases
ATGGCTGTAACAAAGATAATCGAGGTCCCATTCACACTAGAGATTCCTGTAGGACCCCAACATCCCGCCCTCCACGAGCC
CGTATTATTGAAGGCTTATGCGGATGGGGAGGAAATAGTAAAGGTAGATATTAACACGGGATATAATCACAGGGGTATCG
AGAAGCTCTGCGAGAAAAACAGCTTCTACCGCGACATATTTATTGTTGGAAGAGTCTGTGGTATATGTAATGCGGTACAC
GCAAATGCTTATGTGAGGGCGCTTGAAGCAATATTAGATGTAACACCTAATAATAGGGCCAGATACCTAAGAGTCCTGGC
GATGGAGCTAGAGCGTATACACAGCCATATGCTGATAAATGCTGTGATGGCTGAAAACATAGGCTTTGAAGCACTATTCA
TGAACATAATGCTAGACCGTGAAAAAGTAATGAAGGCCAAGGAGATCGTGACAGGTGCCAGAGTTATGGCGGACTACATG
ATGGTTGGCGGCGTTAGAAGAGACATAGATGACGTCAAGAGGGATAAACTGAGAAAGCTTATCTCAGAGCTGAGGCCAAG
GGTTGAGTACTATAGGAAGGTATTCGAGGAAGACGAAACAATCTTGAAGAGACTAATCGATGTAGGCAGGCTTAAACCAG
TGGATGTCACAAGCCATGGGTTACTTGGCCCTGTTGCAAGGGCTTCTGGTGTAAAGATAGATACAAGGGCCAGTGACAGG
TATGATGCGTATAATGAGATTCCATTCGACGTTGTAACCAGGAATGAAGGGGATTCATGGGCTAGAATGATGGTTAGATG
GGATGAGGTCCTTGAAGCCATAAACATGGTAGAGTATATCCTGGAGAAACTACCCAGCGACGGTAATCCAGTACCGGATG
AGAAGAAGCTGCCGAGGAAATTCCCGCTTGGAGAGGCGTATACAAGGGTTGAGGCTCAAAGAGGTGAGCTGACATACTAT
GTCATGAGTGATGGTAAGGGTATGAATCCCTACAGGGTTAAAATAAGGACTCCCAGTTTTAATAATATAATAAACACGGG
TTTCATGTATATTGGCCACACAATACCTGATCTTCCTGTAATACTTACATCTCTCGACCCATGTATTTCATGTATGGAGA
GAGTCACTGTTGTGGACTTGGAGAAGAATATCGTGTACAAGGTTTCCTTGAAGGAGCTGGGTGGTGGGAAATGGGGAAGC
CGAAGATACTCAATCTAG

Upstream 100 bases:

>100_bases
TTCAAGGGTAATTCATTCTTAAAGCGCGGCTTCTTCATCCCGCAGGATTTAGTTGAACAGGATAGGTATCCCCTGAGAAA
AGATAGCGGGGTGTAGACGT

Downstream 100 bases:

>100_bases
TATTAAAGAACCTGGCTAGTAAGCCAGCTACCATACAGTATCCCAAGGAACCGACCCCTGTGGAGCCCGATTACAGGGGG
AGGCATTACGCGGATCTAGG

Product: NADH-ubiquinone oxidoreductase, chain 49 kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAVTKIIEVPFTLEIPVGPQHPALHEPVLLKAYADGEEIVKVDINTGYNHRGIEKLCEKNSFYRDIFIVGRVCGICNAVH
ANAYVRALEAILDVTPNNRARYLRVLAMELERIHSHMLINAVMAENIGFEALFMNIMLDREKVMKAKEIVTGARVMADYM
MVGGVRRDIDDVKRDKLRKLISELRPRVEYYRKVFEEDETILKRLIDVGRLKPVDVTSHGLLGPVARASGVKIDTRASDR
YDAYNEIPFDVVTRNEGDSWARMMVRWDEVLEAINMVEYILEKLPSDGNPVPDEKKLPRKFPLGEAYTRVEAQRGELTYY
VMSDGKGMNPYRVKIRTPSFNNIINTGFMYIGHTIPDLPVILTSLDPCISCMERVTVVDLEKNIVYKVSLKELGGGKWGS
RRYSI

Sequences:

>Translated_405_residues
MAVTKIIEVPFTLEIPVGPQHPALHEPVLLKAYADGEEIVKVDINTGYNHRGIEKLCEKNSFYRDIFIVGRVCGICNAVH
ANAYVRALEAILDVTPNNRARYLRVLAMELERIHSHMLINAVMAENIGFEALFMNIMLDREKVMKAKEIVTGARVMADYM
MVGGVRRDIDDVKRDKLRKLISELRPRVEYYRKVFEEDETILKRLIDVGRLKPVDVTSHGLLGPVARASGVKIDTRASDR
YDAYNEIPFDVVTRNEGDSWARMMVRWDEVLEAINMVEYILEKLPSDGNPVPDEKKLPRKFPLGEAYTRVEAQRGELTYY
VMSDGKGMNPYRVKIRTPSFNNIINTGFMYIGHTIPDLPVILTSLDPCISCMERVTVVDLEKNIVYKVSLKELGGGKWGS
RRYSI
>Mature_404_residues
AVTKIIEVPFTLEIPVGPQHPALHEPVLLKAYADGEEIVKVDINTGYNHRGIEKLCEKNSFYRDIFIVGRVCGICNAVHA
NAYVRALEAILDVTPNNRARYLRVLAMELERIHSHMLINAVMAENIGFEALFMNIMLDREKVMKAKEIVTGARVMADYMM
VGGVRRDIDDVKRDKLRKLISELRPRVEYYRKVFEEDETILKRLIDVGRLKPVDVTSHGLLGPVARASGVKIDTRASDRY
DAYNEIPFDVVTRNEGDSWARMMVRWDEVLEAINMVEYILEKLPSDGNPVPDEKKLPRKFPLGEAYTRVEAQRGELTYYV
MSDGKGMNPYRVKIRTPSFNNIINTGFMYIGHTIPDLPVILTSLDPCISCMERVTVVDLEKNIVYKVSLKELGGGKWGSR
RYSI

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=372, Percent_Identity=29.3010752688172, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI260898743, Length=370, Percent_Identity=29.1891891891892, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1789076, Length=392, Percent_Identity=35.4591836734694, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788832, Length=380, Percent_Identity=33.9473684210526, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI145693162, Length=382, Percent_Identity=27.7486910994764, Blast_Score=158, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17568379, Length=380, Percent_Identity=29.2105263157895, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17555284, Length=380, Percent_Identity=28.6842105263158, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24638644, Length=385, Percent_Identity=28.8311688311688, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI221459469, Length=376, Percent_Identity=30.5851063829787, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 46167; Mature: 46036

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTKIIEVPFTLEIPVGPQHPALHEPVLLKAYADGEEIVKVDINTGYNHRGIEKLCEKN
CCCCEEEECCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHCC
SFYRDIFIVGRVCGICNAVHANAYVRALEAILDVTPNNRARYLRVLAMELERIHSHMLIN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVMAENIGFEALFMNIMLDREKVMKAKEIVTGARVMADYMMVGGVRRDIDDVKRDKLRKL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ISELRPRVEYYRKVFEEDETILKRLIDVGRLKPVDVTSHGLLGPVARASGVKIDTRASDR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCC
YDAYNEIPFDVVTRNEGDSWARMMVRWDEVLEAINMVEYILEKLPSDGNPVPDEKKLPRK
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
FPLGEAYTRVEAQRGELTYYVMSDGKGMNPYRVKIRTPSFNNIINTGFMYIGHTIPDLPV
CCCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHEECCCCCCHHH
ILTSLDPCISCMERVTVVDLEKNIVYKVSLKELGGGKWGSRRYSI
HHHHHHHHHHHHHHCEEEEECCCEEEEEEHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AVTKIIEVPFTLEIPVGPQHPALHEPVLLKAYADGEEIVKVDINTGYNHRGIEKLCEKN
CCCEEEECCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHCC
SFYRDIFIVGRVCGICNAVHANAYVRALEAILDVTPNNRARYLRVLAMELERIHSHMLIN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVMAENIGFEALFMNIMLDREKVMKAKEIVTGARVMADYMMVGGVRRDIDDVKRDKLRKL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ISELRPRVEYYRKVFEEDETILKRLIDVGRLKPVDVTSHGLLGPVARASGVKIDTRASDR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCC
YDAYNEIPFDVVTRNEGDSWARMMVRWDEVLEAINMVEYILEKLPSDGNPVPDEKKLPRK
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
FPLGEAYTRVEAQRGELTYYVMSDGKGMNPYRVKIRTPSFNNIINTGFMYIGHTIPDLPV
CCCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHEECCCCCCHHH
ILTSLDPCISCMERVTVVDLEKNIVYKVSLKELGGGKWGSRRYSI
HHHHHHHHHHHHHHCEEEEECCCEEEEEEHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]