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The map label for this gene is pgk [H]

Identifier: 218883497

GI number: 218883497

Start: 171877

End: 173160

Strand: Direct

Name: pgk [H]

Synonym: DKAM_0184

Alternate gene names: 218883497

Gene position: 171877-173160 (Clockwise)

Preceding gene: 218883496

Following gene: 218883498

Centisome position: 12.59

GC content: 46.11

Gene sequence:

>1284_bases
TTGGAAGTTATAAACGCTGGCGTTAATGGAATAAATGGTGGAACAATGAGCCACTTAGAGATCCCCAGGCTACCCACAAT
AAGGGATATTGATATAACGGGTAGAAAAGTCTTCATGCGCATAGATATAAATGTCCCCATAGATCCCGACACAAGTGAAA
TACTTGATGATCGACGGATTAGAACACATGCTTCTTTTATCAAAAGACTAGTGGAGGAGTATAGGCCTGCACTGGTACTT
GCATCACACCAGGGTAGACCCGGTGACAGCGATTTTACATCAATGGAGAAGCATGCCGAGTTACTATCAAAGTACACTGG
GATAAACATACGTTACATTCCTGACGTAATAGGTCCAAAGGCTCTTGAGGAAATAAATAACTTAAAGCCAGGCGAGATCC
TCTTGCTGGATAACCTGAGGCTCCTTTCAGAGGAAGTATTAGAGGCTCCACCCGAGAGGCAGGCATTAACTATTATGGCC
AGGAGGCTTTCATCGGTGATCAACATATATGTGAACGATGCATTTGCAACAGCTCACAGGAGCCAGCCAAGCATCGTGGG
ATTACCACTCCTCATGCCGAGTTGCATCGGCCCCCTGTTCGAGTGTGAGATAGAGGCAGTTAGAAAGGTATTCGGCTCCG
GTGAGTCGCCCAGGATATATGTTCTAGGCGGCTCCAAGGTTAGGGAATTATTAAGGGTCATCGAGAACCTGATGAGGAAT
AAGTTAGCCGATAGAATACTTACAACAGGCCTCCTAGCACATTTATTCCTAGTCGCCAAGGGAGTGAACATAGGGACAGA
GAACATGAAAATCCTGGAGGAGAAAGGGGTACTATCCCTTGTCCCCAGGGCTAGATATATACTGCTACGGGGAGCACCGG
TTGAGACACCAGTTGATCTCAAGGTTGAAGTAAACGGTGATGTGAGGAACACATATATAGGTGAGGTTAGAGGTGTGGCT
AAGGATATAGGTGAAAACACAGTCCAGATATACAGCGACCTCATAAGGGATGCTAGAATCATAGTTATGCGTGGTCCCGC
AGGCGTTATAGAGGATGAGAGATTTAGAGATGGAACACTTAGGATACTCGACGCTGTATACAGTTCCAACGCTTTTACAT
TGATAGCTGGAGGACACCTGGCCTCTATGGTGAATGAATCAAAGTTATCGAATAGAATACATGTGTCAACAGGCGGCAAC
GCACTCCTACTATTCCTCTCCGGCGAAGAACTCCCAGCGTTAAAGGCACTGGAGTTATCGGCTAAAATGTTCCTGGGATG
GTAG

Upstream 100 bases:

>100_bases
CATCATAATTTTTCTTAATTCTTCATTTTATTGTGTTTATGTAAAAAATCATGTAAGAATTCTCGTTGATTAGTTTTAAA
TATTTAAATAAACCCTTCCA

Downstream 100 bases:

>100_bases
CCATGTCTGTTAAAGTAGCTGTAAATGGCTCGGGAACCATAGGTAAGAGAATTGTTGACTCAGTATTGTTGAACAAGGAC
TTTAAGCTAGTTGGAGTCGC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MEVINAGVNGINGGTMSHLEIPRLPTIRDIDITGRKVFMRIDINVPIDPDTSEILDDRRIRTHASFIKRLVEEYRPALVL
ASHQGRPGDSDFTSMEKHAELLSKYTGINIRYIPDVIGPKALEEINNLKPGEILLLDNLRLLSEEVLEAPPERQALTIMA
RRLSSVINIYVNDAFATAHRSQPSIVGLPLLMPSCIGPLFECEIEAVRKVFGSGESPRIYVLGGSKVRELLRVIENLMRN
KLADRILTTGLLAHLFLVAKGVNIGTENMKILEEKGVLSLVPRARYILLRGAPVETPVDLKVEVNGDVRNTYIGEVRGVA
KDIGENTVQIYSDLIRDARIIVMRGPAGVIEDERFRDGTLRILDAVYSSNAFTLIAGGHLASMVNESKLSNRIHVSTGGN
ALLLFLSGEELPALKALELSAKMFLGW

Sequences:

>Translated_427_residues
MEVINAGVNGINGGTMSHLEIPRLPTIRDIDITGRKVFMRIDINVPIDPDTSEILDDRRIRTHASFIKRLVEEYRPALVL
ASHQGRPGDSDFTSMEKHAELLSKYTGINIRYIPDVIGPKALEEINNLKPGEILLLDNLRLLSEEVLEAPPERQALTIMA
RRLSSVINIYVNDAFATAHRSQPSIVGLPLLMPSCIGPLFECEIEAVRKVFGSGESPRIYVLGGSKVRELLRVIENLMRN
KLADRILTTGLLAHLFLVAKGVNIGTENMKILEEKGVLSLVPRARYILLRGAPVETPVDLKVEVNGDVRNTYIGEVRGVA
KDIGENTVQIYSDLIRDARIIVMRGPAGVIEDERFRDGTLRILDAVYSSNAFTLIAGGHLASMVNESKLSNRIHVSTGGN
ALLLFLSGEELPALKALELSAKMFLGW
>Mature_427_residues
MEVINAGVNGINGGTMSHLEIPRLPTIRDIDITGRKVFMRIDINVPIDPDTSEILDDRRIRTHASFIKRLVEEYRPALVL
ASHQGRPGDSDFTSMEKHAELLSKYTGINIRYIPDVIGPKALEEINNLKPGEILLLDNLRLLSEEVLEAPPERQALTIMA
RRLSSVINIYVNDAFATAHRSQPSIVGLPLLMPSCIGPLFECEIEAVRKVFGSGESPRIYVLGGSKVRELLRVIENLMRN
KLADRILTTGLLAHLFLVAKGVNIGTENMKILEEKGVLSLVPRARYILLRGAPVETPVDLKVEVNGDVRNTYIGEVRGVA
KDIGENTVQIYSDLIRDARIIVMRGPAGVIEDERFRDGTLRILDAVYSSNAFTLIAGGHLASMVNESKLSNRIHVSTGGN
ALLLFLSGEELPALKALELSAKMFLGW

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=419, Percent_Identity=30.3102625298329, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI31543397, Length=422, Percent_Identity=29.6208530805687, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI1789294, Length=409, Percent_Identity=28.8508557457213, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17508823, Length=418, Percent_Identity=29.4258373205742, Blast_Score=161, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=30.5084745762712, Blast_Score=169, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24581203, Length=422, Percent_Identity=30.5687203791469, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17136394, Length=421, Percent_Identity=29.2161520190024, Blast_Score=148, Evalue=9e-36,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 47148; Mature: 47148

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVINAGVNGINGGTMSHLEIPRLPTIRDIDITGRKVFMRIDINVPIDPDTSEILDDRRI
CCEEECCCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHH
RTHASFIKRLVEEYRPALVLASHQGRPGDSDFTSMEKHAELLSKYTGINIRYIPDVIGPK
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHH
ALEEINNLKPGEILLLDNLRLLSEEVLEAPPERQALTIMARRLSSVINIYVNDAFATAHR
HHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCHHHHHCC
SQPSIVGLPLLMPSCIGPLFECEIEAVRKVFGSGESPRIYVLGGSKVRELLRVIENLMRN
CCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHH
KLADRILTTGLLAHLFLVAKGVNIGTENMKILEEKGVLSLVPRARYILLRGAPVETPVDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCCCEEEEEECCCCCCCCEE
KVEVNGDVRNTYIGEVRGVAKDIGENTVQIYSDLIRDARIIVMRGPAGVIEDERFRDGTL
EEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHH
RILDAVYSSNAFTLIAGGHLASMVNESKLSNRIHVSTGGNALLLFLSGEELPALKALELS
HHHHHHHCCCCEEEEECCHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCHHHHHHH
AKMFLGW
HHHHCCC
>Mature Secondary Structure
MEVINAGVNGINGGTMSHLEIPRLPTIRDIDITGRKVFMRIDINVPIDPDTSEILDDRRI
CCEEECCCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHH
RTHASFIKRLVEEYRPALVLASHQGRPGDSDFTSMEKHAELLSKYTGINIRYIPDVIGPK
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHH
ALEEINNLKPGEILLLDNLRLLSEEVLEAPPERQALTIMARRLSSVINIYVNDAFATAHR
HHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCHHHHHCC
SQPSIVGLPLLMPSCIGPLFECEIEAVRKVFGSGESPRIYVLGGSKVRELLRVIENLMRN
CCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHH
KLADRILTTGLLAHLFLVAKGVNIGTENMKILEEKGVLSLVPRARYILLRGAPVETPVDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCCCEEEEEECCCCCCCCEE
KVEVNGDVRNTYIGEVRGVAKDIGENTVQIYSDLIRDARIIVMRGPAGVIEDERFRDGTL
EEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHH
RILDAVYSSNAFTLIAGGHLASMVNESKLSNRIHVSTGGNALLLFLSGEELPALKALELS
HHHHHHHCCCCEEEEECCHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCHHHHHHH
AKMFLGW
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]