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The map label for this gene is gabT [C]

Identifier: 218883438

GI number: 218883438

Start: 111196

End: 112542

Strand: Direct

Name: gabT [C]

Synonym: DKAM_0124

Alternate gene names: 218883438

Gene position: 111196-112542 (Clockwise)

Preceding gene: 218883435

Following gene: 218883440

Centisome position: 8.14

GC content: 46.77

Gene sequence:

>1347_bases
TTGAAGCCCGAGGTAAGAGTAGAGCCAGGCTCACCAGGCAGTAAGTCTAGATACTGGATAGAGGAACACTTCAAATACGT
TGCAACAACAACTCATAGCCCAGAAGAACAACCCCTGGTAATCGAGAGAGGAGAGGGTGTATGGTTATATGATGTAGATG
GAAACAAGTACCTTGATTTCTCAAGCGCCATAGGAGTAAACAACCTAGGTTATCCAACACATCCAGAGGTAAAGAAGGCT
GTTGTAGAGCAGTTAGAAAAGCTCGCCCATGGAGCCGGCACAGACTTCTTCAACCCATACCAGGTCATGCTGGCTAAGAG
ACTCGCCGAGCTAGCCCCTGGGGGTATACCTAGGAAGGTGTTCTTCTCGAATAGCGGTACAGAGGCTAATGAAGCTGCTT
TAAAGATAGCTAGACAGGCAACTGGGAGAAAGTTCTTCATAGCATTCTTTGGTGGATTCCACGGGCGGACAATGGGATCC
CTGGGATTAACGGCTAGTAAAAACATTCACAAGAAACTGGTATTCCCATGGATGCCCGGGGTATTTCATGCACCATACCC
TAATCCATATAGGAATCCCTGGCATATCGACGGCTATGAGGAGCCTGCTGAACTAGTCAATAGAGTTATAGAGTTCATAG
AGGAATACATACTTGATAAACTCGTACCACCCGATGAGGTTGCCGCAATATTCGTTGAACCCATACAGGGCGAAGGTGGA
TACGTGGTGCCTCCACCAATGTTCTTCATGGAGTTGAAGAAATTGGCCGATAAGTATGGGATACTGATATCGATTGATGA
GGTGCAAATGGGCGTTGGAAGAACCGGTAAAATGTTCGCTATAGAGCATTTTGGAATAACCCCTGACATCATATCAATGG
CTAAGGCGTTAAGCGGTGGCGTTGTACCGATAGGTGCCACTATATTCAGGAGCGAGCTCGACTTTAAACAACCCGGGATA
CATAGCAACACCTACGGTGGTCATGCCTTTGCATCAATAGCAGCATTGAAAACCCTGGAAGTGGTTGAGAGGCTACTTCC
ACACGTGGGAAGACTCGAATCATTGTTCAGGGATGAGTTAAATGAGTTGAAGCACGAAGTCGAACAAGTAGGGGATGTAA
GGGGTATCGGGTTAGCATGGGGTGTTGAAATAGTTAGGGATAAAAGCTCTAAGACTCCTTATCCAGCGCTTAGGAACAAG
ATTGTATCCAAGACTCTGAGGAATGGACTAGTGCTACTGCCCTGCGGGAAATCCTCGATAAGGCTTATCCCGCCATTAGT
GATAAGCGAGGAGGAAGCTAAAACAGGGTTAGAGATATTCAAGAAGTCTGTGAAGGAGTCGCTGTAG

Upstream 100 bases:

>100_bases
CCCGGTTTCCAATGGAGCTTAACGGTTGAATAGTATTTATAGTCTATGCCATATGTATTTATATGTGTAGATATATATAG
GATGTAGAGGTGTTGAATTA

Downstream 100 bases:

>100_bases
TCTAATGTTTTTACTTAAGATATTTGAACATGTATGGGCTTTCCGGTGGGTGGAAATACCCTAGTCTCCTATAGTACTCC
CTGGCGCCGACACCGCTTAT

Product: 4-aminobutyrate aminotransferase

Products: succinate semialdehyde; L-glutamate

Alternate protein names: ORF B [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKPEVRVEPGSPGSKSRYWIEEHFKYVATTTHSPEEQPLVIERGEGVWLYDVDGNKYLDFSSAIGVNNLGYPTHPEVKKA
VVEQLEKLAHGAGTDFFNPYQVMLAKRLAELAPGGIPRKVFFSNSGTEANEAALKIARQATGRKFFIAFFGGFHGRTMGS
LGLTASKNIHKKLVFPWMPGVFHAPYPNPYRNPWHIDGYEEPAELVNRVIEFIEEYILDKLVPPDEVAAIFVEPIQGEGG
YVVPPPMFFMELKKLADKYGILISIDEVQMGVGRTGKMFAIEHFGITPDIISMAKALSGGVVPIGATIFRSELDFKQPGI
HSNTYGGHAFASIAALKTLEVVERLLPHVGRLESLFRDELNELKHEVEQVGDVRGIGLAWGVEIVRDKSSKTPYPALRNK
IVSKTLRNGLVLLPCGKSSIRLIPPLVISEEEAKTGLEIFKKSVKESL

Sequences:

>Translated_448_residues
MKPEVRVEPGSPGSKSRYWIEEHFKYVATTTHSPEEQPLVIERGEGVWLYDVDGNKYLDFSSAIGVNNLGYPTHPEVKKA
VVEQLEKLAHGAGTDFFNPYQVMLAKRLAELAPGGIPRKVFFSNSGTEANEAALKIARQATGRKFFIAFFGGFHGRTMGS
LGLTASKNIHKKLVFPWMPGVFHAPYPNPYRNPWHIDGYEEPAELVNRVIEFIEEYILDKLVPPDEVAAIFVEPIQGEGG
YVVPPPMFFMELKKLADKYGILISIDEVQMGVGRTGKMFAIEHFGITPDIISMAKALSGGVVPIGATIFRSELDFKQPGI
HSNTYGGHAFASIAALKTLEVVERLLPHVGRLESLFRDELNELKHEVEQVGDVRGIGLAWGVEIVRDKSSKTPYPALRNK
IVSKTLRNGLVLLPCGKSSIRLIPPLVISEEEAKTGLEIFKKSVKESL
>Mature_448_residues
MKPEVRVEPGSPGSKSRYWIEEHFKYVATTTHSPEEQPLVIERGEGVWLYDVDGNKYLDFSSAIGVNNLGYPTHPEVKKA
VVEQLEKLAHGAGTDFFNPYQVMLAKRLAELAPGGIPRKVFFSNSGTEANEAALKIARQATGRKFFIAFFGGFHGRTMGS
LGLTASKNIHKKLVFPWMPGVFHAPYPNPYRNPWHIDGYEEPAELVNRVIEFIEEYILDKLVPPDEVAAIFVEPIQGEGG
YVVPPPMFFMELKKLADKYGILISIDEVQMGVGRTGKMFAIEHFGITPDIISMAKALSGGVVPIGATIFRSELDFKQPGI
HSNTYGGHAFASIAALKTLEVVERLLPHVGRLESLFRDELNELKHEVEQVGDVRGIGLAWGVEIVRDKSSKTPYPALRNK
IVSKTLRNGLVLLPCGKSSIRLIPPLVISEEEAKTGLEIFKKSVKESL

Specific function: 4-aminobutyrate (GABA) degradation. [C]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=449, Percent_Identity=31.8485523385301, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI13994255, Length=455, Percent_Identity=31.2087912087912, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI37574042, Length=414, Percent_Identity=28.9855072463768, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI226442705, Length=412, Percent_Identity=28.6407766990291, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI24119277, Length=416, Percent_Identity=30.2884615384615, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI226442709, Length=377, Percent_Identity=29.1777188328912, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI284507298, Length=332, Percent_Identity=32.5301204819277, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI188536080, Length=437, Percent_Identity=28.8329519450801, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI38679950, Length=437, Percent_Identity=28.8329519450801, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI38679946, Length=437, Percent_Identity=28.8329519450801, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1789016, Length=421, Percent_Identity=36.104513064133, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI145693181, Length=407, Percent_Identity=36.6093366093366, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1787560, Length=411, Percent_Identity=35.036496350365, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1788044, Length=417, Percent_Identity=34.2925659472422, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1789759, Length=414, Percent_Identity=30.9178743961353, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786991, Length=419, Percent_Identity=28.1622911694511, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1786349, Length=312, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564660, Length=432, Percent_Identity=32.1759259259259, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI25144271, Length=457, Percent_Identity=30.1969365426696, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71992977, Length=424, Percent_Identity=30.4245283018868, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI25144274, Length=297, Percent_Identity=31.3131313131313, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17541228, Length=476, Percent_Identity=27.9411764705882, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323470, Length=445, Percent_Identity=33.0337078651685, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6324432, Length=442, Percent_Identity=31.6742081447964, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321456, Length=454, Percent_Identity=28.6343612334802, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324386, Length=404, Percent_Identity=25, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28574759, Length=437, Percent_Identity=32.9519450800915, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI161085790, Length=433, Percent_Identity=32.5635103926097, Blast_Score=189, Evalue=5e-48,
Organism=Drosophila melanogaster, GI21357415, Length=450, Percent_Identity=31.1111111111111, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21356575, Length=419, Percent_Identity=30.0715990453461, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI281366494, Length=444, Percent_Identity=28.6036036036036, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24667139, Length=444, Percent_Identity=28.6036036036036, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24667143, Length=444, Percent_Identity=28.6036036036036, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19

Molecular weight: Translated: 49649; Mature: 49649

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEVRVEPGSPGSKSRYWIEEHFKYVATTTHSPEEQPLVIERGEGVWLYDVDGNKYLDF
CCCCEEECCCCCCCCCCHHHHHHHHHHEECCCCCCCCCEEEECCCEEEEEECCCCEEEEH
SSAIGVNNLGYPTHPEVKKAVVEQLEKLAHGAGTDFFNPYQVMLAKRLAELAPGGIPRKV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
FFSNSGTEANEAALKIARQATGRKFFIAFFGGFHGRTMGSLGLTASKNIHKKLVFPWMPG
EECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCC
VFHAPYPNPYRNPWHIDGYEEPAELVNRVIEFIEEYILDKLVPPDEVAAIFVEPIQGEGG
CEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
YVVPPPMFFMELKKLADKYGILISIDEVQMGVGRTGKMFAIEHFGITPDIISMAKALSGG
EECCCHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
VVPIGATIFRSELDFKQPGIHSNTYGGHAFASIAALKTLEVVERLLPHVGRLESLFRDEL
CEEECHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NELKHEVEQVGDVRGIGLAWGVEIVRDKSSKTPYPALRNKIVSKTLRNGLVLLPCGKSSI
HHHHHHHHHHCCCCCCCHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCC
RLIPPLVISEEEAKTGLEIFKKSVKESL
EECCCEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPEVRVEPGSPGSKSRYWIEEHFKYVATTTHSPEEQPLVIERGEGVWLYDVDGNKYLDF
CCCCEEECCCCCCCCCCHHHHHHHHHHEECCCCCCCCCEEEECCCEEEEEECCCCEEEEH
SSAIGVNNLGYPTHPEVKKAVVEQLEKLAHGAGTDFFNPYQVMLAKRLAELAPGGIPRKV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
FFSNSGTEANEAALKIARQATGRKFFIAFFGGFHGRTMGSLGLTASKNIHKKLVFPWMPG
EECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCC
VFHAPYPNPYRNPWHIDGYEEPAELVNRVIEFIEEYILDKLVPPDEVAAIFVEPIQGEGG
CEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
YVVPPPMFFMELKKLADKYGILISIDEVQMGVGRTGKMFAIEHFGITPDIISMAKALSGG
EECCCHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
VVPIGATIFRSELDFKQPGIHSNTYGGHAFASIAALKTLEVVERLLPHVGRLESLFRDEL
CEEECHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NELKHEVEQVGDVRGIGLAWGVEIVRDKSSKTPYPALRNKIVSKTLRNGLVLLPCGKSSI
HHHHHHHHHHCCCCCCCHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCC
RLIPPLVISEEEAKTGLEIFKKSVKESL
EECCCEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-aminobutanoate; 2-oxoglutarate

Specific reaction: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA