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The map label for this gene is tilS [H]

Identifier: 218533183

GI number: 218533183

Start: 5688429

End: 5689472

Strand: Reverse

Name: tilS [H]

Synonym: Mchl_5309

Alternate gene names: 218533183

Gene position: 5689472-5688429 (Counterclockwise)

Preceding gene: 218533184

Following gene: 218533182

Centisome position: 98.47

GC content: 72.7

Gene sequence:

>1044_bases
TTGAGCGCAGCGCCCGCGTCCCTCGACGAACGCCTCGCCCGCGCCCTCGCACCATTCCTCGAAGAACCCGCTCGCCCAGC
CCTGCTCGCCGTCTCGGGCGGACCCGATTCCACCGCGCTGATGCACGCGGCCGCCCGAGCGGGGCGGGACCTTCTCCACG
TCGCGACGGTCGACCACGCCCTCCGGCCGGGTTCGGCCGATGAGGCGCTCGGGGTCGGGCGCCTTGCCGCCGAACTCGGC
CTTGTCCACACGATTCTGACTTGGGACGCGCCGCGACATGGCAGCCGGATCCAGGCGTCGGCGCGCGGTGCCCGCTACAA
GCTCCTGACCGACCATGCCGCACGGATCGGCGCGGGCCTCATTCTCACCGCGCACACGCTGGACGATCAGGCCGAGACCG
TGCTGATGCGGCTCATCGCCGGAAGCGGGCCCTCCGGGCTCGCCGGGATGCGGGACGAGCGTCCGCTGGCACCGGGCATC
CGGCTGGTGCGGCCCTTCTTGAGCCTCTCGAAGGCCGACCTCGTGGCCTATTGCGAGATGCACCGCCTCCCGTTCCTGCG
CGATCCCTCCAACACGGACGAGCGCTTCACCCGAGCCCGGTTGCGCCGCCTTCTGCCGCTCCTGGCGGAGGAAGGCCTGA
ACGCGGCGAGGCTGACCCGCCTTGCCGTTCGCGCCGGGCGGGACGACGCGGCGCTGAGGCAGCGGGCCGAGGCCGTCCTG
GCGGAGATCACGCGCACGGACGAGGCAAGCACAGACGCGGCAAGGCTGCGTCTCGACGGGCAACGCCTGCGTCGCGAACC
CTGCGCCATCGTCCTGCGCGTCGTCGATGCGGCTCTCGATAGGGTCGCACCGCCTCACGAAGCCCCCGTTCCGAAGCGCC
TGGAACGCCTCGAAGCGCTCGTTCTCGATGCGCTGCTGCCGGCTCTGGGCGAGGGGAGGGCGTTGCGCCGCACCCTCCGC
GGCGTCCTGATCGAGGCGGACGGAGAGGGGACGATCCGCCTTGTCGCGGCGCCGCCGCGCAAGGCGCCGGACCGCCGCCG
CTAA

Upstream 100 bases:

>100_bases
CCTGCGCCACGCTGGCCGAGGTCGAGCGCAAGTTTCCGAGCGCCGGCGCCACCGTGCGCCAGGGCGTCGAGCGCGAGCAG
AAGCGCGCCCGCTGCGCCGC

Downstream 100 bases:

>100_bases
CGTTACGCGGTCGCCCTCGACGCAGGGGCAGCCGATTTACTTGGCAACCGGTGGGGCGCCGCCTACATTGGCCTGGAGTG
CGTGGGGTATGCCGCGACAT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSAAPASLDERLARALAPFLEEPARPALLAVSGGPDSTALMHAAARAGRDLLHVATVDHALRPGSADEALGVGRLAAELG
LVHTILTWDAPRHGSRIQASARGARYKLLTDHAARIGAGLILTAHTLDDQAETVLMRLIAGSGPSGLAGMRDERPLAPGI
RLVRPFLSLSKADLVAYCEMHRLPFLRDPSNTDERFTRARLRRLLPLLAEEGLNAARLTRLAVRAGRDDAALRQRAEAVL
AEITRTDEASTDAARLRLDGQRLRREPCAIVLRVVDAALDRVAPPHEAPVPKRLERLEALVLDALLPALGEGRALRRTLR
GVLIEADGEGTIRLVAAPPRKAPDRRR

Sequences:

>Translated_347_residues
MSAAPASLDERLARALAPFLEEPARPALLAVSGGPDSTALMHAAARAGRDLLHVATVDHALRPGSADEALGVGRLAAELG
LVHTILTWDAPRHGSRIQASARGARYKLLTDHAARIGAGLILTAHTLDDQAETVLMRLIAGSGPSGLAGMRDERPLAPGI
RLVRPFLSLSKADLVAYCEMHRLPFLRDPSNTDERFTRARLRRLLPLLAEEGLNAARLTRLAVRAGRDDAALRQRAEAVL
AEITRTDEASTDAARLRLDGQRLRREPCAIVLRVVDAALDRVAPPHEAPVPKRLERLEALVLDALLPALGEGRALRRTLR
GVLIEADGEGTIRLVAAPPRKAPDRRR
>Mature_346_residues
SAAPASLDERLARALAPFLEEPARPALLAVSGGPDSTALMHAAARAGRDLLHVATVDHALRPGSADEALGVGRLAAELGL
VHTILTWDAPRHGSRIQASARGARYKLLTDHAARIGAGLILTAHTLDDQAETVLMRLIAGSGPSGLAGMRDERPLAPGIR
LVRPFLSLSKADLVAYCEMHRLPFLRDPSNTDERFTRARLRRLLPLLAEEGLNAARLTRLAVRAGRDDAALRQRAEAVLA
EITRTDEASTDAARLRLDGQRLRREPCAIVLRVVDAALDRVAPPHEAPVPKRLERLEALVLDALLPALGEGRALRRTLRG
VLIEADGEGTIRLVAAPPRKAPDRRR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=197, Percent_Identity=32.994923857868, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 37423; Mature: 37292

Theoretical pI: Translated: 11.05; Mature: 11.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAPASLDERLARALAPFLEEPARPALLAVSGGPDSTALMHAAARAGRDLLHVATVDHA
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHH
LRPGSADEALGVGRLAAELGLVHTILTWDAPRHGSRIQASARGARYKLLTDHAARIGAGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHCCCCEEEHHHHHHHHHCCCE
ILTAHTLDDQAETVLMRLIAGSGPSGLAGMRDERPLAPGIRLVRPFLSLSKADLVAYCEM
EEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HRLPFLRDPSNTDERFTRARLRRLLPLLAEEGLNAARLTRLAVRAGRDDAALRQRAEAVL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
AEITRTDEASTDAARLRLDGQRLRREPCAIVLRVVDAALDRVAPPHEAPVPKRLERLEAL
HHHHHCCCCCCCHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VLDALLPALGEGRALRRTLRGVLIEADGEGTIRLVAAPPRKAPDRRR
HHHHHHHHHCCCHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SAAPASLDERLARALAPFLEEPARPALLAVSGGPDSTALMHAAARAGRDLLHVATVDHA
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHH
LRPGSADEALGVGRLAAELGLVHTILTWDAPRHGSRIQASARGARYKLLTDHAARIGAGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHCCCCEEEHHHHHHHHHCCCE
ILTAHTLDDQAETVLMRLIAGSGPSGLAGMRDERPLAPGIRLVRPFLSLSKADLVAYCEM
EEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HRLPFLRDPSNTDERFTRARLRRLLPLLAEEGLNAARLTRLAVRAGRDDAALRQRAEAVL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
AEITRTDEASTDAARLRLDGQRLRREPCAIVLRVVDAALDRVAPPHEAPVPKRLERLEAL
HHHHHCCCCCCCHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VLDALLPALGEGRALRRTLRGVLIEADGEGTIRLVAAPPRKAPDRRR
HHHHHHHHHCCCHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA