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The map label for this gene is ruvB [H]

Identifier: 218533109

GI number: 218533109

Start: 5602471

End: 5603541

Strand: Reverse

Name: ruvB [H]

Synonym: Mchl_5235

Alternate gene names: 218533109

Gene position: 5603541-5602471 (Counterclockwise)

Preceding gene: 218533112

Following gene: 218533108

Centisome position: 96.98

GC content: 69.19

Gene sequence:

>1071_bases
GTGAGCGAAGACAAGACCTCCAAGCCCGGCAAGACCCTGCTGAGCCCCGAGCGCCGGCCGGACGACGCCGACCAGTCGAT
CCGCCCGTTGAGCCTTTCCGAGTTCATCGGGCAGCGGGCGGCGCGTGCCAACATGCAGATCTTCATCGAGGCGGCCAAGA
AGACCGGCCAGGCCCTCGATCACGTCCTGTTCGTCGGCCCGCCGGGCCTCGGCAAGACGACGCTGGCACAGATCGTCGCC
CGCGAACTCGGCGTCAATTTCCGCTCGACCTCCGGCCCGGTGATCGCCAAGGCCGGCGATCTGGCGGCGCAGCTCACCAA
TCTGGAGGAGCGCGACGTCCTGTTCATCGACGAGATCCACCGTCTCAACCCGGCGGTGGAAGAGATCCTTTATCCCGCGA
TGGAGGATTACCAGCTCGACCTCATCATCGGCGAAGGCCCGGCCGCCCGCTCGGTGAAGATCGAGCTGCCGAAATTCACC
CTCGTCGGCGCCACCACCCGCGCGGGTCTGCTCACCACGCCGCTGCGCGACCGCTTCGGCATCCCGATCCGCCTCGAATT
CTACGAGATCGACGAACTGGAACTCATCGTCCGCCGCGGCGCGCGGGTGCTCGGCCTCGGGATGTCGGAGGAGGGCGCCA
ACGAGATCGCCAAGCGAGCCCGCGGCACCCCGCGCATCGCCGGGCGCCTGCTGCGCCGGGTGCGCGACTTCGCCATCGTC
GAGGACGCGACGACGGTGAGCCGCGCCATCGCCGACCGGGCGCTGCAGATGCTCGACGTCGATCCCGTTGGCCTCGACGT
GATGGACCGCAAGTACCTCACCCTGATCGCCGCCTCGTTCGGCGGCGGCCCGGTCGGGATCGAGACCATCGCCGCGGCCC
TCTCCGAGCCGCGCGACGCGATCGAGGACATCATCGAGCCCTACCTGATCCAGAAGGGCTTCGTGCAGCGCACCCCGCGC
GGGCGGGTGCTGACACCGCACGCCTTCCGGCACATGGGCTTTGCCGAACCGCGGCGCGATCCCGGCTCGCAGTTCGGCCT
GTTCGGCGGCAGCGACGACCCGGATGCGTGA

Upstream 100 bases:

>100_bases
ATCCCTCACGAGACGCCCCGCCGCCGGCCGTTTCCCCTCGGGGCGACAGCGCAGCGGGCGTTTTGCGAGAGCCATTCGGC
CGACGCCCCGCGAGACCCCC

Downstream 100 bases:

>100_bases
CGGCCGAAGCCCCGTTTCCGTCACGCGACGGACGGTGCTGATCGGGCTCGGCGCGACGGCGCTGACCGGGGTGGGGACGG
GCGCCTATGCAGTCGGCATC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSEDKTSKPGKTLLSPERRPDDADQSIRPLSLSEFIGQRAARANMQIFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVA
RELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFT
LVGATTRAGLLTTPLRDRFGIPIRLEFYEIDELELIVRRGARVLGLGMSEEGANEIAKRARGTPRIAGRLLRRVRDFAIV
EDATTVSRAIADRALQMLDVDPVGLDVMDRKYLTLIAASFGGGPVGIETIAAALSEPRDAIEDIIEPYLIQKGFVQRTPR
GRVLTPHAFRHMGFAEPRRDPGSQFGLFGGSDDPDA

Sequences:

>Translated_356_residues
MSEDKTSKPGKTLLSPERRPDDADQSIRPLSLSEFIGQRAARANMQIFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVA
RELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFT
LVGATTRAGLLTTPLRDRFGIPIRLEFYEIDELELIVRRGARVLGLGMSEEGANEIAKRARGTPRIAGRLLRRVRDFAIV
EDATTVSRAIADRALQMLDVDPVGLDVMDRKYLTLIAASFGGGPVGIETIAAALSEPRDAIEDIIEPYLIQKGFVQRTPR
GRVLTPHAFRHMGFAEPRRDPGSQFGLFGGSDDPDA
>Mature_355_residues
SEDKTSKPGKTLLSPERRPDDADQSIRPLSLSEFIGQRAARANMQIFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVAR
ELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTL
VGATTRAGLLTTPLRDRFGIPIRLEFYEIDELELIVRRGARVLGLGMSEEGANEIAKRARGTPRIAGRLLRRVRDFAIVE
DATTVSRAIADRALQMLDVDPVGLDVMDRKYLTLIAASFGGGPVGIETIAAALSEPRDAIEDIIEPYLIQKGFVQRTPRG
RVLTPHAFRHMGFAEPRRDPGSQFGLFGGSDDPDA

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=334, Percent_Identity=64.3712574850299, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787119, Length=146, Percent_Identity=32.1917808219178, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 38897; Mature: 38766

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDKTSKPGKTLLSPERRPDDADQSIRPLSLSEFIGQRAARANMQIFIEAAKKTGQALD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHH
HVLFVGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIH
CEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCEEEECHHHHHHHHCCCCCCEEHHHHHH
RLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLVGATTRAGLLTTPLRDRFG
HCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCCCCCEEECCHHHHCC
IPIRLEFYEIDELELIVRRGARVLGLGMSEEGANEIAKRARGTPRIAGRLLRRVRDFAIV
CCEEEEEEECCHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EDATTVSRAIADRALQMLDVDPVGLDVMDRKYLTLIAASFGGGPVGIETIAAALSEPRDA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEHHCCCCCCCHHHHHHHHHCHHHH
IEDIIEPYLIQKGFVQRTPRGRVLTPHAFRHMGFAEPRRDPGSQFGLFGGSDDPDA
HHHHHHHHHHHHHHHHCCCCCCEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEDKTSKPGKTLLSPERRPDDADQSIRPLSLSEFIGQRAARANMQIFIEAAKKTGQALD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHH
HVLFVGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIH
CEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCEEEECHHHHHHHHCCCCCCEEHHHHHH
RLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLVGATTRAGLLTTPLRDRFG
HCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCCCCCEEECCHHHHCC
IPIRLEFYEIDELELIVRRGARVLGLGMSEEGANEIAKRARGTPRIAGRLLRRVRDFAIV
CCEEEEEEECCHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EDATTVSRAIADRALQMLDVDPVGLDVMDRKYLTLIAASFGGGPVGIETIAAALSEPRDA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEHHCCCCCCCHHHHHHHHHCHHHH
IEDIIEPYLIQKGFVQRTPRGRVLTPHAFRHMGFAEPRRDPGSQFGLFGGSDDPDA
HHHHHHHHHHHHHHHHCCCCCCEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]