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The map label for this gene is 218533079

Identifier: 218533079

GI number: 218533079

Start: 5569711

End: 5570763

Strand: Reverse

Name: 218533079

Synonym: Mchl_5205

Alternate gene names: NA

Gene position: 5570763-5569711 (Counterclockwise)

Preceding gene: 218533080

Following gene: 218533077

Centisome position: 96.41

GC content: 75.88

Gene sequence:

>1053_bases
GTGACAGGTGAGACGGATTTCGTCCGGCGGATCGGGCTCGTGCATCCGATCATCCAGGCTCCCATGGTCGGCCCGAAGGC
GGCGCTCGCCGCCGCCGTTAGCGGGGCGGGCGGGCTCGGCTCCCTTGCCTGCGCCGCGCTCACGCCCGATCAGATCCGCG
CCGAGGTGACCGCGATCAGGGCCGTGACGGAGGCGCCGTTCAACCTGAACTTCTTCTGCCACGAGCCGCCCGCACCCGAT
CCGGCACGCGAGGCCGCGTGGCGGGCCGCCCTCGCGCCCTACTACGCGGCGTTCGGGCTCGACCCGGCGGCGCCGGTGGC
GATGGCGAACCGCGCGCCCTTCGACGCCGCGATGGCCGAGGTGGTGGAAGAGCTGCGCCCGGCCGTCGTCAGCTTCCATT
TCGGCCTGCCGGCGGAGCCGCTGCTGCGGCGGGTGCGGGACGCCGGCTGCCTGATCTGGTCCTCGGCCACCACGGTGCGC
GAGGCGCGCTGGCTCGCCGAACGCGGCGTCGATGCGGTGATCGCGCAAGGGGCGGAGGCCGGCGGCCACCGCGGTATGTT
CCTCGCCGACGCGGCCGCCTCTCAGGTCGGCACCATCGCCCTCGTGCCGCAGATCGCCGACGCCGTGGATGTGCCGGTGA
TCGCGGCCGGCGGCATCGGCGATCCCCGCGGCGTTGCGGCCGCCTTCGCGCTGGGCGCCAGCGCCGTGCAGGTCGGCACC
GCCTATCTGCGCTGCCCGGAGGCCGGCATTTCCGCGCCCTATCGTGCGGCGCTGGCGGCGGCCCGCGACGAGGACACGGC
GCTCACCAACGTGCTGACCGGGCGCCCGGCCCGCGGCCTGCTCACCCGCGCCGTGCGCGAGCTCGGACCCATCAGCCCGC
TCGCTCCGGCCTTTCCCGGCGCGGCGGTGGCGCTCCAGCCCCTGCGCACGGCCGCGGAGGCGGCGGGCTCGGGCGATTTC
TCGCCGCTCTGGTCCGGGCAGGCGGTCGGCCTCTCGCAGGAGCGGCCGGCGGCGGAACTGACCCGCCTGCTGGCGAGCCG
CGTGCCGAGCTAA

Upstream 100 bases:

>100_bases
GCTCCGCCTCGGCGACGATCCCTCCGCTGACCGGGATCTGGCCCCGCGCCCCGTCCGGCCTCGTCCCGCCACTCATATTG
GGGCGAGTGAGGAGACAGCC

Downstream 100 bases:

>100_bases
AGCGCATTCCGACGAAGTGGCCACCGGTTCGTTGAAAGAATGCGCGCCACAAGAATGACTTGGAGCCGCAACGAAAAAGG
GCCGCCCCGAGGGGCGGCCC

Product: 2-nitropropane dioxygenase NPD

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTGETDFVRRIGLVHPIIQAPMVGPKAALAAAVSGAGGLGSLACAALTPDQIRAEVTAIRAVTEAPFNLNFFCHEPPAPD
PAREAAWRAALAPYYAAFGLDPAAPVAMANRAPFDAAMAEVVEELRPAVVSFHFGLPAEPLLRRVRDAGCLIWSSATTVR
EARWLAERGVDAVIAQGAEAGGHRGMFLADAAASQVGTIALVPQIADAVDVPVIAAGGIGDPRGVAAAFALGASAVQVGT
AYLRCPEAGISAPYRAALAAARDEDTALTNVLTGRPARGLLTRAVRELGPISPLAPAFPGAAVALQPLRTAAEAAGSGDF
SPLWSGQAVGLSQERPAAELTRLLASRVPS

Sequences:

>Translated_350_residues
MTGETDFVRRIGLVHPIIQAPMVGPKAALAAAVSGAGGLGSLACAALTPDQIRAEVTAIRAVTEAPFNLNFFCHEPPAPD
PAREAAWRAALAPYYAAFGLDPAAPVAMANRAPFDAAMAEVVEELRPAVVSFHFGLPAEPLLRRVRDAGCLIWSSATTVR
EARWLAERGVDAVIAQGAEAGGHRGMFLADAAASQVGTIALVPQIADAVDVPVIAAGGIGDPRGVAAAFALGASAVQVGT
AYLRCPEAGISAPYRAALAAARDEDTALTNVLTGRPARGLLTRAVRELGPISPLAPAFPGAAVALQPLRTAAEAAGSGDF
SPLWSGQAVGLSQERPAAELTRLLASRVPS
>Mature_349_residues
TGETDFVRRIGLVHPIIQAPMVGPKAALAAAVSGAGGLGSLACAALTPDQIRAEVTAIRAVTEAPFNLNFFCHEPPAPDP
AREAAWRAALAPYYAAFGLDPAAPVAMANRAPFDAAMAEVVEELRPAVVSFHFGLPAEPLLRRVRDAGCLIWSSATTVRE
ARWLAERGVDAVIAQGAEAGGHRGMFLADAAASQVGTIALVPQIADAVDVPVIAAGGIGDPRGVAAAFALGASAVQVGTA
YLRCPEAGISAPYRAALAAARDEDTALTNVLTGRPARGLLTRAVRELGPISPLAPAFPGAAVALQPLRTAAEAAGSGDFS
PLWSGQAVGLSQERPAAELTRLLASRVPS

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=318, Percent_Identity=32.3899371069182, Blast_Score=150, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 35748; Mature: 35617

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGETDFVRRIGLVHPIIQAPMVGPKAALAAAVSGAGGLGSLACAALTPDQIRAEVTAIR
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHH
AVTEAPFNLNFFCHEPPAPDPAREAAWRAALAPYYAAFGLDPAAPVAMANRAPFDAAMAE
HHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHH
VVEELRPAVVSFHFGLPAEPLLRRVRDAGCLIWSSATTVREARWLAERGVDAVIAQGAEA
HHHHHHHHHEEEECCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCHHHHHHCCCCC
GGHRGMFLADAAASQVGTIALVPQIADAVDVPVIAAGGIGDPRGVAAAFALGASAVQVGT
CCCCCEEEEHHHHHHCCEEEEHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
AYLRCPEAGISAPYRAALAAARDEDTALTNVLTGRPARGLLTRAVRELGPISPLAPAFPG
HHEECCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
AAVALQPLRTAAEAAGSGDFSPLWSGQAVGLSQERPAAELTRLLASRVPS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGETDFVRRIGLVHPIIQAPMVGPKAALAAAVSGAGGLGSLACAALTPDQIRAEVTAIR
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHH
AVTEAPFNLNFFCHEPPAPDPAREAAWRAALAPYYAAFGLDPAAPVAMANRAPFDAAMAE
HHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHH
VVEELRPAVVSFHFGLPAEPLLRRVRDAGCLIWSSATTVREARWLAERGVDAVIAQGAEA
HHHHHHHHHEEEECCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCHHHHHHCCCCC
GGHRGMFLADAAASQVGTIALVPQIADAVDVPVIAAGGIGDPRGVAAAFALGASAVQVGT
CCCCCEEEEHHHHHHCCEEEEHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
AYLRCPEAGISAPYRAALAAARDEDTALTNVLTGRPARGLLTRAVRELGPISPLAPAFPG
HHEECCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
AAVALQPLRTAAEAAGSGDFSPLWSGQAVGLSQERPAAELTRLLASRVPS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA