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The map label for this gene is mltB [H]

Identifier: 218533077

GI number: 218533077

Start: 5567316

End: 5568512

Strand: Reverse

Name: mltB [H]

Synonym: Mchl_5203

Alternate gene names: 218533077

Gene position: 5568512-5567316 (Counterclockwise)

Preceding gene: 218533079

Following gene: 218533071

Centisome position: 96.38

GC content: 70.51

Gene sequence:

>1197_bases
ATGCCGACGCCAGCCACCCGCGCCAGCCTGATCGGGCTTGCCCTTGCCAGCGGCCTTCTCGCCGGCCCCGCGCGAGCCGA
TTTCCAATCTTGCCTTGCCGGCATCCAGGCGCAGGCCGCCGGGGCCGGCGTCTCGGCCCAGACCTTCCGCGCCGCGACGG
CCAACATCGCCTACGACGACAAGGTGATCGAGCTGTCCCAGGCGCAGCCGGAATTCAAGACGCCGATCTGGGACTACATG
TCGGCGCTGGTCGATGAGGAGCGGGTCGAGGACGGGCGCGCGGCCATGCGCCAGCATGCCCAGGCCTTGGCGAACGCGGA
GGCACGCTACGGCGTCGATCGTCACACCATCGCCGCGGTGTGGGGCGTCGAGTCCAATTTCGGCAAGAACCTCGGCAAGA
TGCCGCTGGTGCAGTCGCTTGCCACGCTCGCCTGCTCGAGCAACCGCCGTCGCGACTTCTTCCGCACCGAACTGATCGCG
ACGCTGAAGATCATCGAGCGCGGCGACATCGAAGCCTCGCGGCTGACCGGCTCTTGGGCCGGCGCCTTCGGCCAGACGCA
GTTCATGCCGACCACCTACCAGCGTCTGGCCGTGGACGGCGACGGCGACGGGCGGCGCGACGTGGTCGATTCGGTGGCCG
ACGCGGTCGCCTCCACCGCCAACTTCCTGCGCGTCGCCAAATGGTCGAACGGCCAGCCCTGGGGCTACGAGGTGCGGCTG
CCGCGCGGCTTCAACGTTGCCGCCGCGGGCCGCAAGAACAAGCACGCCGTCGGCCATTGGGCCTCGCTCGGCGTCACCCG
AGTCGATGGCAAGGCGCTGACGGGGGAGGGGCCTGCCGGCATCATCGCGCCCGCCGGTATCAACGGCCCGGCCTTCCTCG
TGACGAAGAACTTCGACGCGATTTATTCCTACAACGCCGCCGAATCCTACGGGCTGGCCATCGCCGTCCTCTCGGACCGC
CTGCGGGGCCGCCCCGGCGTCCAGGCCGACTGGCCGACCGACGATCCGCCGCTCTCGCGTGCCGAGCGCCGCGACCTCCA
GACCCGCCTGACCGCTCGCGGCTACGATGTCGGCGAGCCGGACGGCAAGGTCGGCTCCAAGACCCGCGAGGCGATCAAGG
AGATCGAGCGCCAACTCGGCATGCCCGCCACCGGGCGGCCGGGCGGCAAGGTGCTGGAGGCGCTGCGGCGGGGGTAA

Upstream 100 bases:

>100_bases
GCTTCGAGCGATGGCCCTGCCCCTATCCTCACCTTTCGTTAACCCTCGTATCGCCTGAATCGCGCGAGAGAATCCCCGAA
CTTTGACGGAGCGCCGACCC

Downstream 100 bases:

>100_bases
CGGCAACCCGGCGCGCCGGATCGTCGGCTTCGGCTATGCGAACGAGCCCGCGTCAATCCGGGAAAAACCGTGCTCGCATC
GTCGCAACGACCCCCTCGGC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MPTPATRASLIGLALASGLLAGPARADFQSCLAGIQAQAAGAGVSAQTFRAATANIAYDDKVIELSQAQPEFKTPIWDYM
SALVDEERVEDGRAAMRQHAQALANAEARYGVDRHTIAAVWGVESNFGKNLGKMPLVQSLATLACSSNRRRDFFRTELIA
TLKIIERGDIEASRLTGSWAGAFGQTQFMPTTYQRLAVDGDGDGRRDVVDSVADAVASTANFLRVAKWSNGQPWGYEVRL
PRGFNVAAAGRKNKHAVGHWASLGVTRVDGKALTGEGPAGIIAPAGINGPAFLVTKNFDAIYSYNAAESYGLAIAVLSDR
LRGRPGVQADWPTDDPPLSRAERRDLQTRLTARGYDVGEPDGKVGSKTREAIKEIERQLGMPATGRPGGKVLEALRRG

Sequences:

>Translated_398_residues
MPTPATRASLIGLALASGLLAGPARADFQSCLAGIQAQAAGAGVSAQTFRAATANIAYDDKVIELSQAQPEFKTPIWDYM
SALVDEERVEDGRAAMRQHAQALANAEARYGVDRHTIAAVWGVESNFGKNLGKMPLVQSLATLACSSNRRRDFFRTELIA
TLKIIERGDIEASRLTGSWAGAFGQTQFMPTTYQRLAVDGDGDGRRDVVDSVADAVASTANFLRVAKWSNGQPWGYEVRL
PRGFNVAAAGRKNKHAVGHWASLGVTRVDGKALTGEGPAGIIAPAGINGPAFLVTKNFDAIYSYNAAESYGLAIAVLSDR
LRGRPGVQADWPTDDPPLSRAERRDLQTRLTARGYDVGEPDGKVGSKTREAIKEIERQLGMPATGRPGGKVLEALRRG
>Mature_397_residues
PTPATRASLIGLALASGLLAGPARADFQSCLAGIQAQAAGAGVSAQTFRAATANIAYDDKVIELSQAQPEFKTPIWDYMS
ALVDEERVEDGRAAMRQHAQALANAEARYGVDRHTIAAVWGVESNFGKNLGKMPLVQSLATLACSSNRRRDFFRTELIAT
LKIIERGDIEASRLTGSWAGAFGQTQFMPTTYQRLAVDGDGDGRRDVVDSVADAVASTANFLRVAKWSNGQPWGYEVRLP
RGFNVAAAGRKNKHAVGHWASLGVTRVDGKALTGEGPAGIIAPAGINGPAFLVTKNFDAIYSYNAAESYGLAIAVLSDRL
RGRPGVQADWPTDDPPLSRAERRDLQTRLTARGYDVGEPDGKVGSKTREAIKEIERQLGMPATGRPGGKVLEALRRG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=246, Percent_Identity=30.8943089430894, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 42534; Mature: 42403

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPATRASLIGLALASGLLAGPARADFQSCLAGIQAQAAGAGVSAQTFRAATANIAYDD
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECC
KVIELSQAQPEFKTPIWDYMSALVDEERVEDGRAAMRQHAQALANAEARYGVDRHTIAAV
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHEEEEE
WGVESNFGKNLGKMPLVQSLATLACSSNRRRDFFRTELIATLKIIERGDIEASRLTGSWA
ECCCCHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCHH
GAFGQTQFMPTTYQRLAVDGDGDGRRDVVDSVADAVASTANFLRVAKWSNGQPWGYEVRL
CCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEC
PRGFNVAAAGRKNKHAVGHWASLGVTRVDGKALTGEGPAGIIAPAGINGPAFLVTKNFDA
CCCCCEEECCCCCCCCCCHHHHCCEEEECCEEECCCCCCCEEECCCCCCCEEEEEECCHH
IYSYNAAESYGLAIAVLSDRLRGRPGVQADWPTDDPPLSRAERRDLQTRLTARGYDVGEP
EEECCCCHHHCEEHEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DGKVGSKTREAIKEIERQLGMPATGRPGGKVLEALRRG
CCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PTPATRASLIGLALASGLLAGPARADFQSCLAGIQAQAAGAGVSAQTFRAATANIAYDD
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECC
KVIELSQAQPEFKTPIWDYMSALVDEERVEDGRAAMRQHAQALANAEARYGVDRHTIAAV
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHEEEEE
WGVESNFGKNLGKMPLVQSLATLACSSNRRRDFFRTELIATLKIIERGDIEASRLTGSWA
ECCCCHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCHH
GAFGQTQFMPTTYQRLAVDGDGDGRRDVVDSVADAVASTANFLRVAKWSNGQPWGYEVRL
CCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEC
PRGFNVAAAGRKNKHAVGHWASLGVTRVDGKALTGEGPAGIIAPAGINGPAFLVTKNFDA
CCCCCEEECCCCCCCCCCHHHHCCEEEECCEEECCCCCCCEEECCCCCCCEEEEEECCHH
IYSYNAAESYGLAIAVLSDRLRGRPGVQADWPTDDPPLSRAERRDLQTRLTARGYDVGEP
EEECCCCHHHCEEHEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DGKVGSKTREAIKEIERQLGMPATGRPGGKVLEALRRG
CCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]