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The map label for this gene is cobD [H]

Identifier: 218533065

GI number: 218533065

Start: 5555657

End: 5556658

Strand: Reverse

Name: cobD [H]

Synonym: Mchl_5191

Alternate gene names: 218533065

Gene position: 5556658-5555657 (Counterclockwise)

Preceding gene: 218533067

Following gene: 218533062

Centisome position: 96.17

GC content: 72.75

Gene sequence:

>1002_bases
ATGTCCTGGATCGCCCTGACCCATCCGCCCGACACCCTCGGCATCCTGGCCCTCGCCCTGCTGATCGAGGCCATGGCGGG
TTATCCGGACCGCCTCTACAGGGCGCTCGGCCATCCTGTCACCTGGATCGGCCGACTGATCGCGGCGCTCGAACGCGGGC
TCAACCGGGGCACGCCGCGAGCGCGCCGGCTCGGCGGCATCCTGGCGCTCACCGGGCTGCTCGCCACGGTGGCCGCCGTC
ACCCTGGGGCTGACGGCACTCGCCGCGCTCATGGGTCACGGGTTCGGGCTGGTGGTCCTCGCGATTCTCGCCGCCAGCCT
GCCGGCGCAGCGCAGCCTGTTCGTCCATGTCCGGCGCGTCTCCGCCGCCCTGCGGACGGAGGGGCTGGCCGGGGGTCGCA
CTGCCGTCTCGATGATCGTCGGCCGAAACCCCGAGAGCCTCGACGAGGCGGCCGTGTGCCGAGCCGCGATCGAGAGCCTC
GCCGAAAACTTTTCCGACGGCATCGTCGCGCCGGCCTTCTGGATCGGCGCGGGCGGGCTCACCGGCGGCGCGCTCTACAA
GGCGATCAACACCGCCGACAGCATGATCGGCCACCGGACTCACCGCTACGAAGCGTTCGGCTGGGCCTCGGCCCGTCTCG
ACGATCTCGTGAACCTGCCGGCCTCGCGGCTCACCGCCCTCCTGCTGATCGCGGCAGCGGCCCTGAGCCGGGATGTCTCT
GCGGCGGGCGCATGGCGGGCGATCCGCCGCGACGCGGGCCGCCACCGCTCGCCCAATGCCGGCTGGCCCGAGGCGGCCAT
GGCCGGCGCGCTTGGCTTACGGCTTGCAGGCCCGCGCATCTACGGCGACACCCGCGTCGAGGATGCCTGGATGGGCGACG
GGCGGGCCGAGGCCAACCCCGACGACATCGTGCGCGCGCTGAAGCTCTACCGGACGGCCTGCGCGCTGCAATTCACGCTG
GTCGCGGCCGGGACCGGCCTTTGGCTCACCTTCGGCCGCTAA

Upstream 100 bases:

>100_bases
CGGGCCGCGTCGAGATCGCCGCCATGCGCGATCCCGTCGCGCGAATCGTCCGTCAGCACCTCGCGTCCGCCCATCCCGCC
CCGCACCGACCCCACCAACG

Downstream 100 bases:

>100_bases
GGTTCTTGTCTTTGCATCATCTTTTTCCGAAAGCCGGCAATCGCCTTTCGGAATGATGCTCGAGCGTCCCTCAGCGCAGG
CGCAGCAGCTCGTCGCAGTC

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSWIALTHPPDTLGILALALLIEAMAGYPDRLYRALGHPVTWIGRLIAALERGLNRGTPRARRLGGILALTGLLATVAAV
TLGLTALAALMGHGFGLVVLAILAASLPAQRSLFVHVRRVSAALRTEGLAGGRTAVSMIVGRNPESLDEAAVCRAAIESL
AENFSDGIVAPAFWIGAGGLTGGALYKAINTADSMIGHRTHRYEAFGWASARLDDLVNLPASRLTALLLIAAAALSRDVS
AAGAWRAIRRDAGRHRSPNAGWPEAAMAGALGLRLAGPRIYGDTRVEDAWMGDGRAEANPDDIVRALKLYRTACALQFTL
VAAGTGLWLTFGR

Sequences:

>Translated_333_residues
MSWIALTHPPDTLGILALALLIEAMAGYPDRLYRALGHPVTWIGRLIAALERGLNRGTPRARRLGGILALTGLLATVAAV
TLGLTALAALMGHGFGLVVLAILAASLPAQRSLFVHVRRVSAALRTEGLAGGRTAVSMIVGRNPESLDEAAVCRAAIESL
AENFSDGIVAPAFWIGAGGLTGGALYKAINTADSMIGHRTHRYEAFGWASARLDDLVNLPASRLTALLLIAAAALSRDVS
AAGAWRAIRRDAGRHRSPNAGWPEAAMAGALGLRLAGPRIYGDTRVEDAWMGDGRAEANPDDIVRALKLYRTACALQFTL
VAAGTGLWLTFGR
>Mature_332_residues
SWIALTHPPDTLGILALALLIEAMAGYPDRLYRALGHPVTWIGRLIAALERGLNRGTPRARRLGGILALTGLLATVAAVT
LGLTALAALMGHGFGLVVLAILAASLPAQRSLFVHVRRVSAALRTEGLAGGRTAVSMIVGRNPESLDEAAVCRAAIESLA
ENFSDGIVAPAFWIGAGGLTGGALYKAINTADSMIGHRTHRYEAFGWASARLDDLVNLPASRLTALLLIAAAALSRDVSA
AGAWRAIRRDAGRHRSPNAGWPEAAMAGALGLRLAGPRIYGDTRVEDAWMGDGRAEANPDDIVRALKLYRTACALQFTLV
AAGTGLWLTFGR

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34975; Mature: 34844

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWIALTHPPDTLGILALALLIEAMAGYPDRLYRALGHPVTWIGRLIAALERGLNRGTPR
CCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCH
ARRLGGILALTGLLATVAAVTLGLTALAALMGHGFGLVVLAILAASLPAQRSLFVHVRRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHH
SAALRTEGLAGGRTAVSMIVGRNPESLDEAAVCRAAIESLAENFSDGIVAPAFWIGAGGL
HHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
TGGALYKAINTADSMIGHRTHRYEAFGWASARLDDLVNLPASRLTALLLIAAAALSRDVS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AAGAWRAIRRDAGRHRSPNAGWPEAAMAGALGLRLAGPRIYGDTRVEDAWMGDGRAEANP
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECCCEECCCCCCCHHHCCCCCCCCCH
DDIVRALKLYRTACALQFTLVAAGTGLWLTFGR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
SWIALTHPPDTLGILALALLIEAMAGYPDRLYRALGHPVTWIGRLIAALERGLNRGTPR
CCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCH
ARRLGGILALTGLLATVAAVTLGLTALAALMGHGFGLVVLAILAASLPAQRSLFVHVRRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHH
SAALRTEGLAGGRTAVSMIVGRNPESLDEAAVCRAAIESLAENFSDGIVAPAFWIGAGGL
HHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
TGGALYKAINTADSMIGHRTHRYEAFGWASARLDDLVNLPASRLTALLLIAAAALSRDVS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AAGAWRAIRRDAGRHRSPNAGWPEAAMAGALGLRLAGPRIYGDTRVEDAWMGDGRAEANP
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECCCEECCCCCCCHHHCCCCCCCCCH
DDIVRALKLYRTACALQFTLVAAGTGLWLTFGR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]