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The map label for this gene is ywbI [H]

Identifier: 218532638

GI number: 218532638

Start: 5042714

End: 5043667

Strand: Reverse

Name: ywbI [H]

Synonym: Mchl_4752

Alternate gene names: 218532638

Gene position: 5043667-5042714 (Counterclockwise)

Preceding gene: 218532641

Following gene: 218532634

Centisome position: 87.29

GC content: 72.43

Gene sequence:

>954_bases
ATGCGCTTCGATCTGACCGACCTGCGCCTGTTCCTTCACGTCGTCGAGGCGGAGAGCATCACCCGTGGCGCCGAGCGGGC
CGGGCTGGCGCTTGCCTCGGCGAGCGAGCGCATCCGCGGCATGGAGGCGGATGGCGGCGTGCCGCTCTTGGAGCGCCATG
CCCGCGGGGTGCGCCCGACGCCGGCGGGCCTCGCCGTGGTGCATCACGCCCGGCTCGTGCTCGGCCAGCTCGAACAGATG
CGCGGCGAACTCGGCCAGTACGCGAGGGGCCTGCGCGGTCATGTCCGGCTGCTGTCGATCACCGCGGCGGTCGCCGCCTT
CCTGCCCGATGCGCTGTCCGGTTTCCTTGCGGCCTATCCGAGCGTCGACATCGATCTCGAAGAGCGCACCAGCCGCGAGA
CCGTCGATGCCCTGGCGGGCGGGCTTGCCGATCTCGGCATCGTCGCCGACACGACCGATCTCGGCGCGCTCGAAACCCGG
CCGCTCCTTCTCGACCGGATCGTGCTCGCAGTGCCGGCGGGCCATGCCCTGGCGCAGCGGAGCGGCGTCGCGTTCCGGGA
GGTGCTGGACGAGCCTCTGGTCGGCCTCAGCTACGGCCGGGCGCTGCAGGCGCATCTCGCGACCCACGCCGCCCGCGCCG
GCCGTCCGTTCAAGCTCCGGGTGCGCCTGAACGGGCTCGATGCGGTCTGCCGCATGGTCGAGCGGGGCGTCGGCGTGGCA
ATCGTGCCCGAGGCGGCGGCGCAGCGCGCGGCCGAGACGCTGGCGATCCACGCGATTCCGATCGACGAGCCCTGGGCCGT
GCGCCAGCTCAGGCTCTGCGCCCGCAGCTTCGCAGCGCTTCCCCCGCACGCCCGCCAACTCGCCGAGCACTTGGTCGCGC
ATCTCGGCGCAGCAGAGAGTCCGGGTTTCGAAAGGACAAGTCCTTTCGCGGGTCCAGGGCAGAGCCCTGGCTGA

Upstream 100 bases:

>100_bases
GCGATGCCACGGGATGGGGCCTTGCCGACGAGGCCCTGGCTTTTCGTCCCTGCATCGTCTTCTTCCGAAAGCTGACGGCC
ATCTTTCGCGACGATGCTCC

Downstream 100 bases:

>100_bases
CATCCGTGATCGACGTCACCAGTCGCGATCAGGCGAGGCGCTCGACCGATACGGGATGCCGCGGAACGCCCTGCTTCTCG
AAGCAGGACAGGTTCTCGAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRFDLTDLRLFLHVVEAESITRGAERAGLALASASERIRGMEADGGVPLLERHARGVRPTPAGLAVVHHARLVLGQLEQM
RGELGQYARGLRGHVRLLSITAAVAAFLPDALSGFLAAYPSVDIDLEERTSRETVDALAGGLADLGIVADTTDLGALETR
PLLLDRIVLAVPAGHALAQRSGVAFREVLDEPLVGLSYGRALQAHLATHAARAGRPFKLRVRLNGLDAVCRMVERGVGVA
IVPEAAAQRAAETLAIHAIPIDEPWAVRQLRLCARSFAALPPHARQLAEHLVAHLGAAESPGFERTSPFAGPGQSPG

Sequences:

>Translated_317_residues
MRFDLTDLRLFLHVVEAESITRGAERAGLALASASERIRGMEADGGVPLLERHARGVRPTPAGLAVVHHARLVLGQLEQM
RGELGQYARGLRGHVRLLSITAAVAAFLPDALSGFLAAYPSVDIDLEERTSRETVDALAGGLADLGIVADTTDLGALETR
PLLLDRIVLAVPAGHALAQRSGVAFREVLDEPLVGLSYGRALQAHLATHAARAGRPFKLRVRLNGLDAVCRMVERGVGVA
IVPEAAAQRAAETLAIHAIPIDEPWAVRQLRLCARSFAALPPHARQLAEHLVAHLGAAESPGFERTSPFAGPGQSPG
>Mature_317_residues
MRFDLTDLRLFLHVVEAESITRGAERAGLALASASERIRGMEADGGVPLLERHARGVRPTPAGLAVVHHARLVLGQLEQM
RGELGQYARGLRGHVRLLSITAAVAAFLPDALSGFLAAYPSVDIDLEERTSRETVDALAGGLADLGIVADTTDLGALETR
PLLLDRIVLAVPAGHALAQRSGVAFREVLDEPLVGLSYGRALQAHLATHAARAGRPFKLRVRLNGLDAVCRMVERGVGVA
IVPEAAAQRAAETLAIHAIPIDEPWAVRQLRLCARSFAALPPHARQLAEHLVAHLGAAESPGFERTSPFAGPGQSPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=26.1904761904762, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI157672245, Length=242, Percent_Identity=28.9256198347107, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790399, Length=287, Percent_Identity=25.0871080139373, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=25.5144032921811, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787879, Length=295, Percent_Identity=25.4237288135593, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=23.5059760956175, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787806, Length=281, Percent_Identity=23.4875444839858, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=28.0632411067194, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789204, Length=283, Percent_Identity=25.4416961130742, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693193, Length=135, Percent_Identity=31.1111111111111, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=252, Percent_Identity=27.7777777777778, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367136, Length=250, Percent_Identity=26.8, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33613; Mature: 33613

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDLTDLRLFLHVVEAESITRGAERAGLALASASERIRGMEADGGVPLLERHARGVRPT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCC
PAGLAVVHHARLVLGQLEQMRGELGQYARGLRGHVRLLSITAAVAAFLPDALSGFLAAYP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SVDIDLEERTSRETVDALAGGLADLGIVADTTDLGALETRPLLLDRIVLAVPAGHALAQR
CCCCCHHHHHHHHHHHHHHCCHHHHCEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
SGVAFREVLDEPLVGLSYGRALQAHLATHAARAGRPFKLRVRLNGLDAVCRMVERGVGVA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCCEE
IVPEAAAQRAAETLAIHAIPIDEPWAVRQLRLCARSFAALPPHARQLAEHLVAHLGAAES
EECHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
PGFERTSPFAGPGQSPG
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRFDLTDLRLFLHVVEAESITRGAERAGLALASASERIRGMEADGGVPLLERHARGVRPT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCC
PAGLAVVHHARLVLGQLEQMRGELGQYARGLRGHVRLLSITAAVAAFLPDALSGFLAAYP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SVDIDLEERTSRETVDALAGGLADLGIVADTTDLGALETRPLLLDRIVLAVPAGHALAQR
CCCCCHHHHHHHHHHHHHHCCHHHHCEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
SGVAFREVLDEPLVGLSYGRALQAHLATHAARAGRPFKLRVRLNGLDAVCRMVERGVGVA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCCEE
IVPEAAAQRAAETLAIHAIPIDEPWAVRQLRLCARSFAALPPHARQLAEHLVAHLGAAES
EECHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
PGFERTSPFAGPGQSPG
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]