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The map label for this gene is yngF [H]

Identifier: 218532493

GI number: 218532493

Start: 4884589

End: 4885923

Strand: Reverse

Name: yngF [H]

Synonym: Mchl_4607

Alternate gene names: 218532493

Gene position: 4885923-4884589 (Counterclockwise)

Preceding gene: 218532500

Following gene: 218532492

Centisome position: 84.56

GC content: 72.43

Gene sequence:

>1335_bases
ATGCGGTCCGGCGCCGTCGCACGCTCCGCGTTGACGCCCCACCCGGGAGTTCGTCTAAGAGGGCCGCGCAATGCCCCGGG
TGCAAAGCCTCCGGGCATAAAGCCCGGCTTCGAAACCGCGGGCATCCGCTCGGGTGACGATGCGGCGAAGGAGAGCGCGG
CGGTGGAGCGCGGAATGTCGGGTGGGCAACAGGCCTTGCAGCAAGGGGTCGCGCAACGGGACGTGCGTGAGCCGTCGGCC
GAGCCCGAGATCGTGTTCGAGACCCGCGGTGAGGCCGGGCTGATCACCCTGACACGGCCGAAGGCGCTCAACGCTCTCAC
GCTGCCGATGGTGGAGGCGATGCACCGGCAGCTTCGGGCCTGGGCGGGGGATGCGCGGGTCAGCCGGATCGTGGTGCGCG
GCTCGGGCGGGCGCGCCTTCTGCGCGGGCGGCGACATCCGCCAGATCCAGGCCCTCGGCAAGGCCGGGCGCCATGCCGAC
GTGATGGCGTTCTGGCGCGGGGAATACTGGCTCGACGCCGCCGTGAAGTCCTACCCGAAGCCCTATATTGCCCTGATCGA
AGGGATCGTGATGGGCGGCGGCGTCGGCATCTCGCTGCACGGCGACGTGCGGGTCGCGGCCGAGAACTACAGCTTCGCCA
TGCCCGAGACCGGCATCGGCTTCTTCCCCGATGTCGGCGCGACCTACGCTCTGCCGCGCCTGCCCGGCTACACCGGGCGC
TACCTCGCGCTGACGGGCGCACGGATCGGCGCGGGCGACGCGCTCGCCGTCGGCCTTGCGACCCACGCGGCGCGGGCGGA
GGATTTCGAGACCATCATCGAGCGGCTGGCGGGGGGAGGGGCGATCGCCCGCGCCTTCGACGGCCTCGATGCGCCGGGGA
CGGAGACCGGCCCCCTCGTCGCCGAGCGGGCGCTGATCGATACGTGCTTCTCCGCCGACACCGTCACGGAGGTGCTCGCC
CGGCTCGACGCGGCAGCGCGGGACGGCTCCGCTTTTGCCGGGGCGACCGCGGCGACGATCCGCACCCGCTCGCCCACCAG
CCTGACCATCGCCCTGGCGCAGATGCGCCGCGGGGCCGACTTGAGCTTTGCCGAAGCAATGCTCGCCGAGTACCGGCTGT
GCGAGCGGGTGATGCGCGGGCACGATTTCTATGAAGGTGTGCGGGCGGTGATCATCGACAAGGACAACCGGCCGGCCTGG
CAGCCCGCGACCCTGGACGCGGTCGATGCCGGGGCGATCGAGGCCGCCTTCGGCCCGCTCGACGGCCCCGAGCCGCTCTT
CGACGGCAGCCAGGGCCCGCCTGAGGCGGCGCGCGCCGCGGAGGCACGACCGTGA

Upstream 100 bases:

>100_bases
CGGACGACAGAGCGCCCCCGAACGGTGCCGCGAGAGAAGCTCCCGCGCGTGCAGATCCTGTCGAAGGGCCCGTTTCCCGA
GGAGCCTCATGAGGCGGTCA

Downstream 100 bases:

>100_bases
GGGCGCTCGCCAAGGTCGGCTCGACCCTCAACGGGCGCGGACGCCCGAGCGCCGGAGCCGGCGACCGGATCGAGGGCACC
GCGCCGCGGGCGCAGACCCG

Product: enoyl-CoA hydratase/isomerase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRSGAVARSALTPHPGVRLRGPRNAPGAKPPGIKPGFETAGIRSGDDAAKESAAVERGMSGGQQALQQGVAQRDVREPSA
EPEIVFETRGEAGLITLTRPKALNALTLPMVEAMHRQLRAWAGDARVSRIVVRGSGGRAFCAGGDIRQIQALGKAGRHAD
VMAFWRGEYWLDAAVKSYPKPYIALIEGIVMGGGVGISLHGDVRVAAENYSFAMPETGIGFFPDVGATYALPRLPGYTGR
YLALTGARIGAGDALAVGLATHAARAEDFETIIERLAGGGAIARAFDGLDAPGTETGPLVAERALIDTCFSADTVTEVLA
RLDAAARDGSAFAGATAATIRTRSPTSLTIALAQMRRGADLSFAEAMLAEYRLCERVMRGHDFYEGVRAVIIDKDNRPAW
QPATLDAVDAGAIEAAFGPLDGPEPLFDGSQGPPEAARAAEARP

Sequences:

>Translated_444_residues
MRSGAVARSALTPHPGVRLRGPRNAPGAKPPGIKPGFETAGIRSGDDAAKESAAVERGMSGGQQALQQGVAQRDVREPSA
EPEIVFETRGEAGLITLTRPKALNALTLPMVEAMHRQLRAWAGDARVSRIVVRGSGGRAFCAGGDIRQIQALGKAGRHAD
VMAFWRGEYWLDAAVKSYPKPYIALIEGIVMGGGVGISLHGDVRVAAENYSFAMPETGIGFFPDVGATYALPRLPGYTGR
YLALTGARIGAGDALAVGLATHAARAEDFETIIERLAGGGAIARAFDGLDAPGTETGPLVAERALIDTCFSADTVTEVLA
RLDAAARDGSAFAGATAATIRTRSPTSLTIALAQMRRGADLSFAEAMLAEYRLCERVMRGHDFYEGVRAVIIDKDNRPAW
QPATLDAVDAGAIEAAFGPLDGPEPLFDGSQGPPEAARAAEARP
>Mature_444_residues
MRSGAVARSALTPHPGVRLRGPRNAPGAKPPGIKPGFETAGIRSGDDAAKESAAVERGMSGGQQALQQGVAQRDVREPSA
EPEIVFETRGEAGLITLTRPKALNALTLPMVEAMHRQLRAWAGDARVSRIVVRGSGGRAFCAGGDIRQIQALGKAGRHAD
VMAFWRGEYWLDAAVKSYPKPYIALIEGIVMGGGVGISLHGDVRVAAENYSFAMPETGIGFFPDVGATYALPRLPGYTGR
YLALTGARIGAGDALAVGLATHAARAEDFETIIERLAGGGAIARAFDGLDAPGTETGPLVAERALIDTCFSADTVTEVLA
RLDAAARDGSAFAGATAATIRTRSPTSLTIALAQMRRGADLSFAEAMLAEYRLCERVMRGHDFYEGVRAVIIDKDNRPAW
QPATLDAVDAGAIEAAFGPLDGPEPLFDGSQGPPEAARAAEARP

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=364, Percent_Identity=43.956043956044, Blast_Score=294, Evalue=9e-80,
Organism=Homo sapiens, GI37594469, Length=315, Percent_Identity=43.1746031746032, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI194097323, Length=195, Percent_Identity=35.3846153846154, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI31542718, Length=238, Percent_Identity=24.7899159663866, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=180, Percent_Identity=32.2222222222222, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI221142681, Length=168, Percent_Identity=34.5238095238095, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082183, Length=192, Percent_Identity=25.5208333333333, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788597, Length=182, Percent_Identity=27.4725274725275, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=378, Percent_Identity=38.3597883597884, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI25144160, Length=356, Percent_Identity=39.6067415730337, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI25145438, Length=171, Percent_Identity=35.0877192982456, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=189, Percent_Identity=32.2751322751323, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=202, Percent_Identity=29.2079207920792, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=199, Percent_Identity=29.1457286432161, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=182, Percent_Identity=24.1758241758242, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=178, Percent_Identity=29.2134831460674, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=350, Percent_Identity=30.5714285714286, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28571730, Length=355, Percent_Identity=40, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI28571729, Length=355, Percent_Identity=40, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI20129971, Length=178, Percent_Identity=33.7078651685393, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653477, Length=178, Percent_Identity=33.7078651685393, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653139, Length=178, Percent_Identity=27.5280898876405, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 46465; Mature: 46465

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSGAVARSALTPHPGVRLRGPRNAPGAKPPGIKPGFETAGIRSGDDAAKESAAVERGMS
CCCCCCHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
GGQQALQQGVAQRDVREPSAEPEIVFETRGEAGLITLTRPKALNALTLPMVEAMHRQLRA
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
WAGDARVSRIVVRGSGGRAFCAGGDIRQIQALGKAGRHADVMAFWRGEYWLDAAVKSYPK
HCCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCC
PYIALIEGIVMGGGVGISLHGDVRVAAENYSFAMPETGIGFFPDVGATYALPRLPGYTGR
HHHHHHHHHHCCCCEEEEEECCEEEEECCCEEECCCCCCCCCCCCCCCEECCCCCCCCCC
YLALTGARIGAGDALAVGLATHAARAEDFETIIERLAGGGAIARAFDGLDAPGTETGPLV
EEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHH
AERALIDTCFSADTVTEVLARLDAAARDGSAFAGATAATIRTRSPTSLTIALAQMRRGAD
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
LSFAEAMLAEYRLCERVMRGHDFYEGVRAVIIDKDNRPAWQPATLDAVDAGAIEAAFGPL
CHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
DGPEPLFDGSQGPPEAARAAEARP
CCCCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MRSGAVARSALTPHPGVRLRGPRNAPGAKPPGIKPGFETAGIRSGDDAAKESAAVERGMS
CCCCCCHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
GGQQALQQGVAQRDVREPSAEPEIVFETRGEAGLITLTRPKALNALTLPMVEAMHRQLRA
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
WAGDARVSRIVVRGSGGRAFCAGGDIRQIQALGKAGRHADVMAFWRGEYWLDAAVKSYPK
HCCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCC
PYIALIEGIVMGGGVGISLHGDVRVAAENYSFAMPETGIGFFPDVGATYALPRLPGYTGR
HHHHHHHHHHCCCCEEEEEECCEEEEECCCEEECCCCCCCCCCCCCCCEECCCCCCCCCC
YLALTGARIGAGDALAVGLATHAARAEDFETIIERLAGGGAIARAFDGLDAPGTETGPLV
EEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHH
AERALIDTCFSADTVTEVLARLDAAARDGSAFAGATAATIRTRSPTSLTIALAQMRRGAD
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
LSFAEAMLAEYRLCERVMRGHDFYEGVRAVIIDKDNRPAWQPATLDAVDAGAIEAAFGPL
CHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
DGPEPLFDGSQGPPEAARAAEARP
CCCCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]