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The map label for this gene is est [H]

Identifier: 218532435

GI number: 218532435

Start: 4816680

End: 4817594

Strand: Reverse

Name: est [H]

Synonym: Mchl_4547

Alternate gene names: 218532435

Gene position: 4817594-4816680 (Counterclockwise)

Preceding gene: 218532436

Following gene: 218532434

Centisome position: 83.38

GC content: 73.88

Gene sequence:

>915_bases
ATGGCCAGCCTGCGCGCCCACCTCTACGATCTTACCATCCGCCTCGCGGTGAAGGGGCGGCTCGGCGGCACCCACGATGT
CTACGCGATCCGCCGCGCCTTCGAGGGCGCCCGGTTCCCGACGCCGGCCGGCGTCGCCTTCACCCCCGGCGAGGTCGGCG
GCGTGCCCGGCGAGTGGGCGGAGGCCGCAGGGGCGGACGGCGAGCGCCCCACCCTGCTCTATTGCCACGGCGGCGGCTTC
ATTGCCTGCTCGGCGCAACTCTACCGCCCGGTCACCGGCCGCTTCGCTCGAGCCGGGTTCCGGGTGTTCACGCCGGATTA
CCGGTTGGCGCCGGAGCACCCCTTCCCCGCGGCGGTCGAGGATTGCACGGGCGTCTGGGCCGCGCTCGCGGAGGCGGGTC
CGGCGGTCGTCGCCGGCGATTCCGCGGGCGGCAGCCTGGCGCTCACCACGATCATCGAGGCGCGCCGCCTCGGCCTGACC
CCGCCCGTGGCGGCGGCCCTGTTCTCACCGGCCACCGACCTGATCGGCCGCAGCGCCTCGTTCCGCACGAATGGGCGGCG
CGACGCGATGTTCCGCCCCGAGGCGCTGATGCGGCTGGTTCCGGCCTACCTCGCCGGGGCCGACCCTGCCGACCCGCGCG
CCTCGCCGATCGAGGCGGATTTTTCCGGGTTTCCGCCGCTCCTGATCCATGTCGGCGTCCGCGAGATCCTGCGCGACGAT
TCCCTGCGCCTCGCCGAGAAGGCGAAGGCCGCGGGTGTGCCGGTCGAACTCACGGTGTTTCCCGTGGTGACGCATGCTTG
GCAGCTCGCCGAGAGCTTTCTGCCCGAGGCACGCCGCTCCCTCGACGAGGCCGCGACCTTCCTGCACCGCGCCCTGAAAG
CGCCCCCCCATCCCGCGGCGGAGGTCGCCGCGTGA

Upstream 100 bases:

>100_bases
TGGTGATCGGCGCCGATGCCCGCCGCGCCCTGCTGATCCAGCGCCTGATGCCGGTGCGCTACTGGTCGCTGATCCGCCGC
GCCATTCGGGACACGTGACC

Downstream 100 bases:

>100_bases
GCGAAGCGCCCCTCGATCTCGTCATCGTCGGCGCCGGCTTTTCCGGTCTCGCCATGGCGATCCGCGCGCGGCAGGCCGGG
TTCGACCGCCTCCTCGTGCT

Product: alpha/beta hydrolase fold-3 domain protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MASLRAHLYDLTIRLAVKGRLGGTHDVYAIRRAFEGARFPTPAGVAFTPGEVGGVPGEWAEAAGADGERPTLLYCHGGGF
IACSAQLYRPVTGRFARAGFRVFTPDYRLAPEHPFPAAVEDCTGVWAALAEAGPAVVAGDSAGGSLALTTIIEARRLGLT
PPVAAALFSPATDLIGRSASFRTNGRRDAMFRPEALMRLVPAYLAGADPADPRASPIEADFSGFPPLLIHVGVREILRDD
SLRLAEKAKAAGVPVELTVFPVVTHAWQLAESFLPEARRSLDEAATFLHRALKAPPHPAAEVAA

Sequences:

>Translated_304_residues
MASLRAHLYDLTIRLAVKGRLGGTHDVYAIRRAFEGARFPTPAGVAFTPGEVGGVPGEWAEAAGADGERPTLLYCHGGGF
IACSAQLYRPVTGRFARAGFRVFTPDYRLAPEHPFPAAVEDCTGVWAALAEAGPAVVAGDSAGGSLALTTIIEARRLGLT
PPVAAALFSPATDLIGRSASFRTNGRRDAMFRPEALMRLVPAYLAGADPADPRASPIEADFSGFPPLLIHVGVREILRDD
SLRLAEKAKAAGVPVELTVFPVVTHAWQLAESFLPEARRSLDEAATFLHRALKAPPHPAAEVAA
>Mature_303_residues
ASLRAHLYDLTIRLAVKGRLGGTHDVYAIRRAFEGARFPTPAGVAFTPGEVGGVPGEWAEAAGADGERPTLLYCHGGGFI
ACSAQLYRPVTGRFARAGFRVFTPDYRLAPEHPFPAAVEDCTGVWAALAEAGPAVVAGDSAGGSLALTTIIEARRLGLTP
PVAAALFSPATDLIGRSASFRTNGRRDAMFRPEALMRLVPAYLAGADPADPRASPIEADFSGFPPLLIHVGVREILRDDS
LRLAEKAKAAGVPVELTVFPVVTHAWQLAESFLPEARRSLDEAATFLHRALKAPPHPAAEVAA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=238, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 32104; Mature: 31973

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLRAHLYDLTIRLAVKGRLGGTHDVYAIRRAFEGARFPTPAGVAFTPGEVGGVPGEWA
CCCHHHHHEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCHHH
EAAGADGERPTLLYCHGGGFIACSAQLYRPVTGRFARAGFRVFTPDYRLAPEHPFPAAVE
HHCCCCCCCCEEEEECCCCEEEECHHHHCCHHHHHHHCCCEEECCCCCCCCCCCCCHHHH
DCTGVWAALAEAGPAVVAGDSAGGSLALTTIIEARRLGLTPPVAAALFSPATDLIGRSAS
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCCCC
FRTNGRRDAMFRPEALMRLVPAYLAGADPADPRASPIEADFSGFPPLLIHVGVREILRDD
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
SLRLAEKAKAAGVPVELTVFPVVTHAWQLAESFLPEARRSLDEAATFLHRALKAPPHPAA
CHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
EVAA
HCCC
>Mature Secondary Structure 
ASLRAHLYDLTIRLAVKGRLGGTHDVYAIRRAFEGARFPTPAGVAFTPGEVGGVPGEWA
CCHHHHHEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCHHH
EAAGADGERPTLLYCHGGGFIACSAQLYRPVTGRFARAGFRVFTPDYRLAPEHPFPAAVE
HHCCCCCCCCEEEEECCCCEEEECHHHHCCHHHHHHHCCCEEECCCCCCCCCCCCCHHHH
DCTGVWAALAEAGPAVVAGDSAGGSLALTTIIEARRLGLTPPVAAALFSPATDLIGRSAS
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCCCC
FRTNGRRDAMFRPEALMRLVPAYLAGADPADPRASPIEADFSGFPPLLIHVGVREILRDD
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
SLRLAEKAKAAGVPVELTVFPVVTHAWQLAESFLPEARRSLDEAATFLHRALKAPPHPAA
CHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
EVAA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]