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The map label for this gene is fliF [H]

Identifier: 218532348

GI number: 218532348

Start: 4719471

End: 4721093

Strand: Reverse

Name: fliF [H]

Synonym: Mchl_4460

Alternate gene names: 218532348

Gene position: 4721093-4719471 (Counterclockwise)

Preceding gene: 218532349

Following gene: 218532347

Centisome position: 81.71

GC content: 70.18

Gene sequence:

>1623_bases
GTGAAACCCATCCTCGATCTGGTGACGAAGCTCGGGCCCGCGCGCATCGCCGCGATGGCGGCCGTGACGCTGACGCTGGT
CGGCTTCTTCGCCTTCGTGATCCTGCGGGTCTCGCGGCCCGATATGGGCGTGCTGTTCTCCGACCTTTCGCTGCAGGATT
CCTCGTCCGTGATCCGCGAACTCGACGCGCGCGGCATCACCTACGAGACCAAGGGTGAGATGGGCCAGACCGTGCTCGCT
CCCCGCGCCGACCTCGCCAAGCTGCGCATGGACTTCGCCGGCAAGGGCCTGCCGTCCCAAGGGGGCATCGGCTACGAGAT
CTTCGACAAGGGCGACGCCTTCTCCTCCACGAGCTTCGTCCAGAACGTGAACCACCTGCGGGCGATGGAGGGCGAACTCG
CCCGCTCGATCCGCGCCATCGGCCGGGTCCAGGCGGCGCGCGTCCACCTCGTCATCCCCGAGCGCCGCCTGTTCGAGCGC
GACCGCGAGGCGCCCTCGGCCGCCATCGTCGTCAAGCTGATGGGCGACCTCGATGCGAGCCAGGTGCGCGCGATCCGGCA
TCTCGCCGCCTCCGCGGTCGAGGGCCTGAAGCCGGAGCGCGTCTCGATCGTCGACGAGCGCGGGCGGCTCCTCGCCGACG
GCGCCCGCGGCGCGGAAGCCGACGGGGCGGGCGCCCTGGAGGAGCGGCAGGCCGGCATCGAACGGCGGATGCGCTCGCAG
ATCGAGGAGATCGTAGCCGGCATCGTCGGCCAGGGCCGCGCCCGGGTGCAGGTCACCGCCGAACTCGACCTCAACCGGGT
CGAGAGCCGCTCGGAGAGCTTCGACCCCGAGAGCCGCGTCGTCCGCTCGACCCAGACGCGCTCCGAGAACTCGCTCACCG
GCGGGGCGGAGGGGCAGGTTACCGTCGGCAACGAATTGCCGGGCGCCAACCAGAACCAGCCCGCGCCGCAGCAGAAGGAT
TCCTCGCAGAAGAACGAGGAGACGACGAATTACGAGATCTCCCGCGTCACCAAGGTCGAGACCCTGGAGGGCGGCCGCCT
CAAGCGTCTGTCGGTGGCGGTGCTGGTCGACGGCGTCTACGCCCCCGGCGCCGACGGCAAGCCCGCCTACCAGCCACGCT
CCGATGCCGAGATCGCACGGATCACGGCGCTGGTGCGCACCGCGGTCGGCTACGACAAGGTCCGCGGCGATCAGGTCGAG
GTGGTCAACCTGCGCTTTGCCGAGACCCCCACCGCCGCGGAGTTTTCCGAGCCCGGCCTGATCCAGTCCTTCCTGAGCCC
GACCAAGGAGGAGGTGATGCGCCTCGTCGAGCTCACGGTGCTGAGCCTGCTCACCCTGGTCGTGCTGATGGCGGTGGTGC
GTCCGCTCCTGCGCCGCGTGCTCGAATCCGATGCGCCGATGGCGCTCGCCGGCCCGGCCGGAGCGCTCGCGCTGGCCGGG
GCGGCGGGCGGCGAGGCGCCGGTCGAGATCGCGGTCCGCGAGAACAGCACTAACAAGTTCCTCGAGTCGGCCAAGATCAA
CGGCCAGATCCAGGCCGAAACGGTCGAGCGCGTGGTCGACATGGTGCGGGCGAGCCCGACCGAGACGGTCGAGGTGCTGC
GCAACTGGATCCACGATTCCTGA

Upstream 100 bases:

>100_bases
GCTCCGGCGTTAACGCCTCGCTAACCACGCACCGGGCAATTCTTGCCGGGCGACCGCGCATCCCTCGCGGCCGCACCGCT
GTGCGCAGGCGTGCCCGTCC

Downstream 100 bases:

>100_bases
TGGACGCTGACACCACGCAGAACCGGAAGAGAGGAGGGAATTCGTGTCCGCGGGCGTAAACTTCAACGCTGCCATGGAGA
GCCTCGATGCGTCGAAGGCG

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKPILDLVTKLGPARIAAMAAVTLTLVGFFAFVILRVSRPDMGVLFSDLSLQDSSSVIRELDARGITYETKGEMGQTVLA
PRADLAKLRMDFAGKGLPSQGGIGYEIFDKGDAFSSTSFVQNVNHLRAMEGELARSIRAIGRVQAARVHLVIPERRLFER
DREAPSAAIVVKLMGDLDASQVRAIRHLAASAVEGLKPERVSIVDERGRLLADGARGAEADGAGALEERQAGIERRMRSQ
IEEIVAGIVGQGRARVQVTAELDLNRVESRSESFDPESRVVRSTQTRSENSLTGGAEGQVTVGNELPGANQNQPAPQQKD
SSQKNEETTNYEISRVTKVETLEGGRLKRLSVAVLVDGVYAPGADGKPAYQPRSDAEIARITALVRTAVGYDKVRGDQVE
VVNLRFAETPTAAEFSEPGLIQSFLSPTKEEVMRLVELTVLSLLTLVVLMAVVRPLLRRVLESDAPMALAGPAGALALAG
AAGGEAPVEIAVRENSTNKFLESAKINGQIQAETVERVVDMVRASPTETVEVLRNWIHDS

Sequences:

>Translated_540_residues
MKPILDLVTKLGPARIAAMAAVTLTLVGFFAFVILRVSRPDMGVLFSDLSLQDSSSVIRELDARGITYETKGEMGQTVLA
PRADLAKLRMDFAGKGLPSQGGIGYEIFDKGDAFSSTSFVQNVNHLRAMEGELARSIRAIGRVQAARVHLVIPERRLFER
DREAPSAAIVVKLMGDLDASQVRAIRHLAASAVEGLKPERVSIVDERGRLLADGARGAEADGAGALEERQAGIERRMRSQ
IEEIVAGIVGQGRARVQVTAELDLNRVESRSESFDPESRVVRSTQTRSENSLTGGAEGQVTVGNELPGANQNQPAPQQKD
SSQKNEETTNYEISRVTKVETLEGGRLKRLSVAVLVDGVYAPGADGKPAYQPRSDAEIARITALVRTAVGYDKVRGDQVE
VVNLRFAETPTAAEFSEPGLIQSFLSPTKEEVMRLVELTVLSLLTLVVLMAVVRPLLRRVLESDAPMALAGPAGALALAG
AAGGEAPVEIAVRENSTNKFLESAKINGQIQAETVERVVDMVRASPTETVEVLRNWIHDS
>Mature_540_residues
MKPILDLVTKLGPARIAAMAAVTLTLVGFFAFVILRVSRPDMGVLFSDLSLQDSSSVIRELDARGITYETKGEMGQTVLA
PRADLAKLRMDFAGKGLPSQGGIGYEIFDKGDAFSSTSFVQNVNHLRAMEGELARSIRAIGRVQAARVHLVIPERRLFER
DREAPSAAIVVKLMGDLDASQVRAIRHLAASAVEGLKPERVSIVDERGRLLADGARGAEADGAGALEERQAGIERRMRSQ
IEEIVAGIVGQGRARVQVTAELDLNRVESRSESFDPESRVVRSTQTRSENSLTGGAEGQVTVGNELPGANQNQPAPQQKD
SSQKNEETTNYEISRVTKVETLEGGRLKRLSVAVLVDGVYAPGADGKPAYQPRSDAEIARITALVRTAVGYDKVRGDQVE
VVNLRFAETPTAAEFSEPGLIQSFLSPTKEEVMRLVELTVLSLLTLVVLMAVVRPLLRRVLESDAPMALAGPAGALALAG
AAGGEAPVEIAVRENSTNKFLESAKINGQIQAETVERVVDMVRASPTETVEVLRNWIHDS

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=425, Percent_Identity=36.2352941176471, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 58186; Mature: 58186

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPILDLVTKLGPARIAAMAAVTLTLVGFFAFVILRVSRPDMGVLFSDLSLQDSSSVIRE
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHH
LDARGITYETKGEMGQTVLAPRADLAKLRMDFAGKGLPSQGGIGYEIFDKGDAFSSTSFV
HHCCCCEEECCCCCCCCHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHH
QNVNHLRAMEGELARSIRAIGRVQAARVHLVIPERRLFERDREAPSAAIVVKLMGDLDAS
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCEEHHHHHHCCCHH
QVRAIRHLAASAVEGLKPERVSIVDERGRLLADGARGAEADGAGALEERQAGIERRMRSQ
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
IEEIVAGIVGQGRARVQVTAELDLNRVESRSESFDPESRVVRSTQTRSENSLTGGAEGQV
HHHHHHHHHCCCCEEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEE
TVGNELPGANQNQPAPQQKDSSQKNEETTNYEISRVTKVETLEGGRLKRLSVAVLVDGVY
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHEEEEEECCCCCCHHEEEEEEEECCC
APGADGKPAYQPRSDAEIARITALVRTAVGYDKVRGDQVEVVNLRFAETPTAAEFSEPGL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCEEEEEEEECCCCCCCCCCCCHH
IQSFLSPTKEEVMRLVELTVLSLLTLVVLMAVVRPLLRRVLESDAPMALAGPAGALALAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHEEC
AAGGEAPVEIAVRENSTNKFLESAKINGQIQAETVERVVDMVRASPTETVEVLRNWIHDS
CCCCCCCEEEEEECCCHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPILDLVTKLGPARIAAMAAVTLTLVGFFAFVILRVSRPDMGVLFSDLSLQDSSSVIRE
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHH
LDARGITYETKGEMGQTVLAPRADLAKLRMDFAGKGLPSQGGIGYEIFDKGDAFSSTSFV
HHCCCCEEECCCCCCCCHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHH
QNVNHLRAMEGELARSIRAIGRVQAARVHLVIPERRLFERDREAPSAAIVVKLMGDLDAS
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCEEHHHHHHCCCHH
QVRAIRHLAASAVEGLKPERVSIVDERGRLLADGARGAEADGAGALEERQAGIERRMRSQ
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
IEEIVAGIVGQGRARVQVTAELDLNRVESRSESFDPESRVVRSTQTRSENSLTGGAEGQV
HHHHHHHHHCCCCEEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEE
TVGNELPGANQNQPAPQQKDSSQKNEETTNYEISRVTKVETLEGGRLKRLSVAVLVDGVY
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHEEEEEECCCCCCHHEEEEEEEECCC
APGADGKPAYQPRSDAEIARITALVRTAVGYDKVRGDQVEVVNLRFAETPTAAEFSEPGL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCEEEEEEEECCCCCCCCCCCCHH
IQSFLSPTKEEVMRLVELTVLSLLTLVVLMAVVRPLLRRVLESDAPMALAGPAGALALAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHEEC
AAGGEAPVEIAVRENSTNKFLESAKINGQIQAETVERVVDMVRASPTETVEVLRNWIHDS
CCCCCCCEEEEEECCCHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002583; 11259647 [H]