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The map label for this gene is pnp

Identifier: 218532284

GI number: 218532284

Start: 4646649

End: 4648886

Strand: Reverse

Name: pnp

Synonym: Mchl_4396

Alternate gene names: 218532284

Gene position: 4648886-4646649 (Counterclockwise)

Preceding gene: 218532285

Following gene: 218532283

Centisome position: 80.46

GC content: 67.52

Gene sequence:

>2238_bases
ATGTTCGACGTACAACGTGAAGAGCTGATCTGGGGCGACCGCAAGCTCGTCCTCGAGACCGGCAAGACCGCCCGCCAGGC
GGACGGCTCCGTGGTCGCCACCTACGGCGAGACCACCGTGCTCGCCACCGTGGTCGCGGGCAAGGAGCCCAAGGCCGGCA
TCGACTTCCTGCCGCTCACCGTGAACTACCAGGAGCGCGCCTACGCTGCCGGCCGCATCCCCGGCGGCTACTTCAAGCGC
GAAGGCCGTCCCTCCGAGAAGGAGACGCTGGTCTCCCGTCTGATCGACCGCCCGATCCGTCCGCTCTTCGTCGAGGGCTG
GCGCAACGACACCCAGGTCGTCGTCACGGTCCTCTCCCACGATCTCGAGAACGATCCCGACATCGTCGCGATGGTCGCGG
CCTCCGCCGCCCTTACCCTCTCGGGCGTGCCCTTCATGGGCCCGATCGGCGCGGCTCGCGTCGGCTACCTCAACGGCGGC
TACAAGCTGAACCCGCTGGTGACGGAAATCGCCGAATCGACCCTCGACCTCGTCGTCGCCGGCACCCAGGATGCGGTGCT
GATGGTCGAGTCCGAGGCCAAGGAACTCTCCGAGGACGTGATGCTCGGGGCCGTGATGTTCGGCCACAAGCATTTCCAGC
CGGTGATCGAGGCGATCATCCGTCTGGCCGAGAAGGCCGCCAAGGAGCCGCGCGACTTCACCCCGCCGGAGAACGCCGAC
GTCGAGGCCGCCGTTCTCGCGGTCGCCGAGACCGAGCTGCGCGAGGCTTACAAGAAGACGGTCAAGCAGGAGCGCTACGC
CGCCGTCGACGCCGTGAAGGCGAAGGTCGTGGCCGCGCTCTGCCCGGCCGAGGGCGAGCAGAAGTTCAGCCCTGAGAAGG
TCAAGGCGGCCTTCAAGGAAGCGCAGTCGAAGGTGGTCCGCTGGAACATCCTCGACACCGGCTCGCGCATCGACGGCCGT
GACGTGAAGACGGTCCGCTCGATCGTCTCCGAGGTCGGCGTGCTGCCCCGCGCCCACGGTTCGTCGCTGTTCACCCGCGG
CGAGACCCAGGCGCTGGTCGTGGCCACGCTCGGCACCGGCGAGGACGAGCAGTTCATCGACGCGCTGGAAGGGACCTACA
AGGAGCGCTTCCTGCTCCACTACAACTTCCCTCCCTACTCCGTCGGCGAGACCGGCCGCATGGGTTCGCCCGGCCGCCGC
GAGATCGGCCACGGCAAGCTCGCCTGGCGCGCGATCCGTCCCGTTCTGCCGCCGGCTCACGAGTTCCCGTACACGATCCG
CGTGGTCTCCGAGATCACCGAGTCGAACGGCTCGTCCTCGATGGCTTCGGTCTGCGGCGGTTCGCTGTCGCTGATGGATG
CGGGCGTGCCCCTGCGCCGCCCGGTCGCCGGCATCGCCATGGGCCTCATCCTCGAAGGTGAGCGCTTCGCCGTGCTGTCC
GACATCCTCGGCGACGAGGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGCTCGGAGGAGGGCATCACCTCCCTCCA
GATGGACATCAAGATCGCCGGCATCACCGAGGAGATCATGAAGATCGCCCTTGCCCAGGCCAAGGACGGCCGCGCCCACA
TCCTGGGCGAGATGTCCAAGGCCCTGACCGCGGCCCGTCCGGAGCTCGGCGAATACGCGCCGCGCATCGAGACGATGCAG
ATCCCCACCGACAAGATCCGTGACGTGATCGGCACCGGCGGCAAGATCATCCGCGAGATCGTCGAGAAGACCGGCGCCAA
GATCAACATCGAGGATACCGGCATCGTCAAGATCGCCTCCTCCGACGGCAAGGCGATCAAGGCGGCCTATAACTGGATCC
GCTCCATCGTCGCCGAGCCCGAGGCCGGCACGATCTACGACGGCACGATCGTCAAGATCATGGAGTTCGGCGCCTTCGTG
AACTTCTTCGGCGCCAAGGACGGCCTCGTCCACATCTCGGAGCTGGCCGCCCAGCGCGTCGCCAAGGTCGGCGACGTCGT
GAAGGAAGGCCAGAAGGTCAAGGTGAAGTTCCTCGGTGCCGACGAGCGCGGCAAGATCCGCCTCTCGATGAAGGTCGTCG
ATCAGGAGACCGGCGAAGACCTCACCGAGAAGCTGAAGGCCGAGCGGGCTGAGCGCGGCGAGCCGGAGCGCGAGGAGCGC
AGCGACCGCGGTGATCGTGGTGACCGTGGCCCCCGCCGCGACCGCGGCGAGCGCCGCCGGGAATCGTCGGGCGAGTAA

Upstream 100 bases:

>100_bases
GGGCAGGATCGCGAGACGCTTCCTTAAGACGAAGCGTCTCGCCGTCTTGCCTCTGGCGGCGCCGGGCTCAGCCCACCGCA
TGTTTGAAGGCGAGAGACAT

Downstream 100 bases:

>100_bases
GCCCCGTCCCGCACGCCATACCGGACGTTTGAAGAGCGCGGTCCCTCCGGGGGCCGCGCTCTTTGCTTGTGGCCCTCGGC
CATTTTGCGCAATGGCAAAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MFDVQREELIWGDRKLVLETGKTARQADGSVVATYGETTVLATVVAGKEPKAGIDFLPLTVNYQERAYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFVEGWRNDTQVVVTVLSHDLENDPDIVAMVAASAALTLSGVPFMGPIGAARVGYLNGG
YKLNPLVTEIAESTLDLVVAGTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFTPPENAD
VEAAVLAVAETELREAYKKTVKQERYAAVDAVKAKVVAALCPAEGEQKFSPEKVKAAFKEAQSKVVRWNILDTGSRIDGR
DVKTVRSIVSEVGVLPRAHGSSLFTRGETQALVVATLGTGEDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPVLPPAHEFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGSEEGITSLQMDIKIAGITEEIMKIALAQAKDGRAHILGEMSKALTAARPELGEYAPRIETMQ
IPTDKIRDVIGTGGKIIREIVEKTGAKINIEDTGIVKIASSDGKAIKAAYNWIRSIVAEPEAGTIYDGTIVKIMEFGAFV
NFFGAKDGLVHISELAAQRVAKVGDVVKEGQKVKVKFLGADERGKIRLSMKVVDQETGEDLTEKLKAERAERGEPEREER
SDRGDRGDRGPRRDRGERRRESSGE

Sequences:

>Translated_745_residues
MFDVQREELIWGDRKLVLETGKTARQADGSVVATYGETTVLATVVAGKEPKAGIDFLPLTVNYQERAYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFVEGWRNDTQVVVTVLSHDLENDPDIVAMVAASAALTLSGVPFMGPIGAARVGYLNGG
YKLNPLVTEIAESTLDLVVAGTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFTPPENAD
VEAAVLAVAETELREAYKKTVKQERYAAVDAVKAKVVAALCPAEGEQKFSPEKVKAAFKEAQSKVVRWNILDTGSRIDGR
DVKTVRSIVSEVGVLPRAHGSSLFTRGETQALVVATLGTGEDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPVLPPAHEFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGSEEGITSLQMDIKIAGITEEIMKIALAQAKDGRAHILGEMSKALTAARPELGEYAPRIETMQ
IPTDKIRDVIGTGGKIIREIVEKTGAKINIEDTGIVKIASSDGKAIKAAYNWIRSIVAEPEAGTIYDGTIVKIMEFGAFV
NFFGAKDGLVHISELAAQRVAKVGDVVKEGQKVKVKFLGADERGKIRLSMKVVDQETGEDLTEKLKAERAERGEPEREER
SDRGDRGDRGPRRDRGERRRESSGE
>Mature_745_residues
MFDVQREELIWGDRKLVLETGKTARQADGSVVATYGETTVLATVVAGKEPKAGIDFLPLTVNYQERAYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFVEGWRNDTQVVVTVLSHDLENDPDIVAMVAASAALTLSGVPFMGPIGAARVGYLNGG
YKLNPLVTEIAESTLDLVVAGTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFTPPENAD
VEAAVLAVAETELREAYKKTVKQERYAAVDAVKAKVVAALCPAEGEQKFSPEKVKAAFKEAQSKVVRWNILDTGSRIDGR
DVKTVRSIVSEVGVLPRAHGSSLFTRGETQALVVATLGTGEDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPVLPPAHEFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGSEEGITSLQMDIKIAGITEEIMKIALAQAKDGRAHILGEMSKALTAARPELGEYAPRIETMQ
IPTDKIRDVIGTGGKIIREIVEKTGAKINIEDTGIVKIASSDGKAIKAAYNWIRSIVAEPEAGTIYDGTIVKIMEFGAFV
NFFGAKDGLVHISELAAQRVAKVGDVVKEGQKVKVKFLGADERGKIRLSMKVVDQETGEDLTEKLKAERAERGEPEREER
SDRGDRGDRGPRRDRGERRRESSGE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=710, Percent_Identity=38.169014084507, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI4826690, Length=88, Percent_Identity=55.6818181818182, Blast_Score=84, Evalue=6e-16,
Organism=Escherichia coli, GI145693187, Length=682, Percent_Identity=58.9442815249267, Blast_Score=780, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=704, Percent_Identity=33.6647727272727, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=87, Percent_Identity=50.5747126436782, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320850, Length=91, Percent_Identity=42.8571428571429, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=37.5354107648725, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=37.5354107648725, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=37.5354107648725, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=673, Percent_Identity=36.849925705795, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI20129977, Length=92, Percent_Identity=47.8260869565217, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_METC4 (B7KN55)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002423100.1
- GeneID:   7117710
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_4396
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 81003; Mature: 81003

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVQREELIWGDRKLVLETGKTARQADGSVVATYGETTVLATVVAGKEPKAGIDFLPLT
CCCCCCHHHHCCCCEEEEECCCCHHHCCCCEEEECCCCEEEEEEEECCCCCCCCEEEEEE
VNYQERAYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFVEGWRNDTQVVVTVLSH
ECHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHEEHHH
DLENDPDIVAMVAASAALTLSGVPFMGPIGAARVGYLNGGYKLNPLVTEIAESTLDLVVA
CCCCCCCEEEHHHHHHHHEECCCCCCCCCCCCCEEEECCCEEECHHHHHHHHHHEEEEEE
GTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFTPPENAD
CCCCEEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VEAAVLAVAETELREAYKKTVKQERYAAVDAVKAKVVAALCPAEGEQKFSPEKVKAAFKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AQSKVVRWNILDTGSRIDGRDVKTVRSIVSEVGVLPRAHGSSLFTRGETQALVVATLGTG
HHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEEECCC
EDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPVLPPAH
CHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
CCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHEECCCHHHHHH
DILGDEDHLGDMDFKVAGSEEGITSLQMDIKIAGITEEIMKIALAQAKDGRAHILGEMSK
HHCCCCCCCCCCCEEECCCCCCCEEEEEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHH
ALTAARPELGEYAPRIETMQIPTDKIRDVIGTGGKIIREIVEKTGAKINIEDTGIVKIAS
HHHHCCCCHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGKAIKAAYNWIRSIVAEPEAGTIYDGTIVKIMEFGAFVNFFGAKDGLVHISELAAQRV
CCCCHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AKVGDVVKEGQKVKVKFLGADERGKIRLSMKVVDQETGEDLTEKLKAERAERGEPEREER
HHHHHHHHCCCEEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHH
SDRGDRGDRGPRRDRGERRRESSGE
HCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MFDVQREELIWGDRKLVLETGKTARQADGSVVATYGETTVLATVVAGKEPKAGIDFLPLT
CCCCCCHHHHCCCCEEEEECCCCHHHCCCCEEEECCCCEEEEEEEECCCCCCCCEEEEEE
VNYQERAYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFVEGWRNDTQVVVTVLSH
ECHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHEEHHH
DLENDPDIVAMVAASAALTLSGVPFMGPIGAARVGYLNGGYKLNPLVTEIAESTLDLVVA
CCCCCCCEEEHHHHHHHHEECCCCCCCCCCCCCEEEECCCEEECHHHHHHHHHHEEEEEE
GTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFTPPENAD
CCCCEEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VEAAVLAVAETELREAYKKTVKQERYAAVDAVKAKVVAALCPAEGEQKFSPEKVKAAFKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AQSKVVRWNILDTGSRIDGRDVKTVRSIVSEVGVLPRAHGSSLFTRGETQALVVATLGTG
HHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEEECCC
EDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPVLPPAH
CHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
CCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHEECCCHHHHHH
DILGDEDHLGDMDFKVAGSEEGITSLQMDIKIAGITEEIMKIALAQAKDGRAHILGEMSK
HHCCCCCCCCCCCEEECCCCCCCEEEEEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHH
ALTAARPELGEYAPRIETMQIPTDKIRDVIGTGGKIIREIVEKTGAKINIEDTGIVKIAS
HHHHCCCCHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGKAIKAAYNWIRSIVAEPEAGTIYDGTIVKIMEFGAFVNFFGAKDGLVHISELAAQRV
CCCCHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AKVGDVVKEGQKVKVKFLGADERGKIRLSMKVVDQETGEDLTEKLKAERAERGEPEREER
HHHHHHHHCCCEEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHH
SDRGDRGDRGPRRDRGERRRESSGE
HCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA