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The map label for this gene is aspS

Identifier: 218532262

GI number: 218532262

Start: 4614810

End: 4616624

Strand: Reverse

Name: aspS

Synonym: Mchl_4374

Alternate gene names: 218532262

Gene position: 4616624-4614810 (Counterclockwise)

Preceding gene: 218532263

Following gene: 218532261

Centisome position: 79.9

GC content: 67.27

Gene sequence:

>1815_bases
ATGCACCGTTACCGTACCCATACCTGCGGGGCGATCCGCCCGTCCGATGTCGGGCAGACCGTCCGCCTGTCCGGCTGGTG
CCACCGCATCCGCGACCATGGCGGCGTGCTCTTCATCGACCTGCGCGACCATTACGGCCTGACGCAATGCGTGATCGATT
CCGACTCGAAGGCCTTCAAGGCGGCCGAGACCGCCCGCTCCGAGTGGGTGATCCGCATCGATGGCCGCGTGCGCACCCGA
CCCGCCGGCACCGAGAACGCGGAGCTGCCGACCGGTAGCGTCGAGGTCTATATCGACGACCTCGAAGTGCTCGGGCCCGC
GGGCGAGCTGCCGCTGCCCGTCTTCGGCGACCAGGAATACCCGGAAGAGACCCGGCTCAAGTACCGCTTCCTCGATCTGC
GCCGGGAGAAGCTGCACGCCAACATCATGAAGCGCGGCGCGATCGTGGATTCGCTCCGCCGCCGCATGCGCGAGGGCGGG
TTCTTCGAGTTCCAGACGCCGATCCTGACCGCCTCTTCGCCGGAGGGCGCGCGCGACTACCTCGTGCCGTCCCGCGTCCA
CCCCGGAAAGTTCTACGCGCTGCCGCAGGCGCCGCAGCAGTTCAAGCAGCTCACGATGATCGCGGGCTTCGATCGCTACT
TCCAGATCGCGCCCTGCTTCCGCGATGAGGATGCCCGCGCCGACCGCTCCCCGGGCGAGTTCTACCAGCTCGACATCGAG
ATGAGCTTCGTCACGCAGGAAGACGTGTTCCAGGCGGTCGAGCCGGTGCTGCGCGGCGTGTTCGAGGAATTCGCTGGCGG
CAAGCGCGTGACGAAGGAGTTCCCGCGCATCACCTACGCCGACGCGATGCTGAAATACGGCGTCGACAAGCCGGACCTGC
GCAACCCGCTGATCATCGCCGACGTGACCGACGAGTTCGCCGACGACGCGGTGGAGTTCAAGGCGTTCAAGGGCGTCATC
AAGTCGGGCGGCGTGGTGCGCGCCATCCCCGCCACCGGCGCCGCCGGCCAGCCGCGCTCGTTCTTCGACAAGCTCAACGA
CTGGGCCCGCTCGGAAGGCGCGCCGGGGCTCGGCTACATCGTGTTCGAGGAAGAGGGTGGGGCGCTCACCGGCAAGGGGC
CGATCGCCAAGTTCATCCCCGCCGCGATCCAGGCGCGGATTGCCGAGAAGGCCGGCGCCAAGGCGGGTGACGCGGTGTTC
TTCGCCGCCGGCACCGAGGCGAAGGCGGCCGGGCTCGCGGGCAAGGCGCGCATCCGCATCGGCGACGAGCTGAAGCTCTC
CGACACGGACCAGTTCGCCTTCTGCTGGGTCGTCGATTTCCCGATGTACGAGTGGAACGAAGAAGACAAGAAAATCGACT
TCTCCCACAACCCGTTCTCGATGCCGAACTTCGACCGGGACGAGTTCCTCGCCCTGGGCGAGGCGGATTCCGAAAAGATC
CTTGGGATTAAGGCGTTCCAGTACGACATCGTCTGCAACGGCATCGAGCTGTCCTCGGGCGCGATCCGGAACCACCGCCC
CGACGTGATGGAGAAGGCGTTTGCCATCGCCGGCTACGGCCGGGACGTGCTGGAGGAGAAGTTCGGCGGCATGCTGAACG
CCCTGCGCCTCGGCGCCCCGCCGCACGGCGGCATCGCGCCGGGTGTCGACCGCATCGTCATGCTGCTCTGCGAGGAGCCG
AACATCCGTGAGGTCGTGCTGTTCCCGATGAACCAGCGCGCCGAGGATCTGATGATGGGCGCGCCCGCCGAGGCGACCCC
GAAGCAGCTGCGCGAGCTGCACATCCGGCTCAACCTGCCCGAGAAGAAGGCGTAG

Upstream 100 bases:

>100_bases
TGACGCTGGCCGCCGCGGAGTAGGGGCACGGCAAGCCGGCCCTCACCTTGACTTGAAACGCCTCCCGTGAATGGTGCCGG
CTCATTCTCAAGAGCCCGCC

Downstream 100 bases:

>100_bases
CGCCTAAACCCTCTCCCACCGCGGGGGAGGGTGCCTGCGGAGCAGGCGGATGAGGGGCCGGTTCCGCTTTGTCGGAAACG
GCGCTCCCCTCGCCCGCCCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MHRYRTHTCGAIRPSDVGQTVRLSGWCHRIRDHGGVLFIDLRDHYGLTQCVIDSDSKAFKAAETARSEWVIRIDGRVRTR
PAGTENAELPTGSVEVYIDDLEVLGPAGELPLPVFGDQEYPEETRLKYRFLDLRREKLHANIMKRGAIVDSLRRRMREGG
FFEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
MSFVTQEDVFQAVEPVLRGVFEEFAGGKRVTKEFPRITYADAMLKYGVDKPDLRNPLIIADVTDEFADDAVEFKAFKGVI
KSGGVVRAIPATGAAGQPRSFFDKLNDWARSEGAPGLGYIVFEEEGGALTGKGPIAKFIPAAIQARIAEKAGAKAGDAVF
FAAGTEAKAAGLAGKARIRIGDELKLSDTDQFAFCWVVDFPMYEWNEEDKKIDFSHNPFSMPNFDRDEFLALGEADSEKI
LGIKAFQYDIVCNGIELSSGAIRNHRPDVMEKAFAIAGYGRDVLEEKFGGMLNALRLGAPPHGGIAPGVDRIVMLLCEEP
NIREVVLFPMNQRAEDLMMGAPAEATPKQLRELHIRLNLPEKKA

Sequences:

>Translated_604_residues
MHRYRTHTCGAIRPSDVGQTVRLSGWCHRIRDHGGVLFIDLRDHYGLTQCVIDSDSKAFKAAETARSEWVIRIDGRVRTR
PAGTENAELPTGSVEVYIDDLEVLGPAGELPLPVFGDQEYPEETRLKYRFLDLRREKLHANIMKRGAIVDSLRRRMREGG
FFEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
MSFVTQEDVFQAVEPVLRGVFEEFAGGKRVTKEFPRITYADAMLKYGVDKPDLRNPLIIADVTDEFADDAVEFKAFKGVI
KSGGVVRAIPATGAAGQPRSFFDKLNDWARSEGAPGLGYIVFEEEGGALTGKGPIAKFIPAAIQARIAEKAGAKAGDAVF
FAAGTEAKAAGLAGKARIRIGDELKLSDTDQFAFCWVVDFPMYEWNEEDKKIDFSHNPFSMPNFDRDEFLALGEADSEKI
LGIKAFQYDIVCNGIELSSGAIRNHRPDVMEKAFAIAGYGRDVLEEKFGGMLNALRLGAPPHGGIAPGVDRIVMLLCEEP
NIREVVLFPMNQRAEDLMMGAPAEATPKQLRELHIRLNLPEKKA
>Mature_604_residues
MHRYRTHTCGAIRPSDVGQTVRLSGWCHRIRDHGGVLFIDLRDHYGLTQCVIDSDSKAFKAAETARSEWVIRIDGRVRTR
PAGTENAELPTGSVEVYIDDLEVLGPAGELPLPVFGDQEYPEETRLKYRFLDLRREKLHANIMKRGAIVDSLRRRMREGG
FFEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
MSFVTQEDVFQAVEPVLRGVFEEFAGGKRVTKEFPRITYADAMLKYGVDKPDLRNPLIIADVTDEFADDAVEFKAFKGVI
KSGGVVRAIPATGAAGQPRSFFDKLNDWARSEGAPGLGYIVFEEEGGALTGKGPIAKFIPAAIQARIAEKAGAKAGDAVF
FAAGTEAKAAGLAGKARIRIGDELKLSDTDQFAFCWVVDFPMYEWNEEDKKIDFSHNPFSMPNFDRDEFLALGEADSEKI
LGIKAFQYDIVCNGIELSSGAIRNHRPDVMEKAFAIAGYGRDVLEEKFGGMLNALRLGAPPHGGIAPGVDRIVMLLCEEP
NIREVVLFPMNQRAEDLMMGAPAEATPKQLRELHIRLNLPEKKA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=36.2438220757825, Blast_Score=358, Evalue=1e-98,
Organism=Homo sapiens, GI45439306, Length=280, Percent_Identity=27.1428571428571, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI226491502, Length=256, Percent_Identity=24.609375, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI4758762, Length=286, Percent_Identity=23.7762237762238, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=596, Percent_Identity=42.6174496644295, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=26.2068965517241, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789256, Length=220, Percent_Identity=28.1818181818182, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=611, Percent_Identity=33.8788870703764, Blast_Score=308, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI71984122, Length=297, Percent_Identity=24.9158249158249, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=275, Percent_Identity=24.3636363636364, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=651, Percent_Identity=29.6466973886329, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321807, Length=297, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323011, Length=267, Percent_Identity=25.8426966292135, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24584738, Length=609, Percent_Identity=34.8111658456486, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI17136276, Length=262, Percent_Identity=27.0992366412214, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19921528, Length=281, Percent_Identity=24.9110320284698, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_METC4 (B7KN33)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002423078.1
- GeneID:   7116538
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_4374
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67191; Mature: 67191

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRTHTCGAIRPSDVGQTVRLSGWCHRIRDHGGVLFIDLRDHYGLTQCVIDSDSKAFK
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHH
AAETARSEWVIRIDGRVRTRPAGTENAELPTGSVEVYIDDLEVLGPAGELPLPVFGDQEY
HHHHCCCCEEEEECCEEEECCCCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCCCCCC
PEETRLKYRFLDLRREKLHANIMKRGAIVDSLRRRMREGGFFEFQTPILTASSPEGARDY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCCCCCC
LVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVFQAVEPVLRGVFEEFAGGKRVTKEFPRITYADAMLKYGVDKPDLRNPLIIA
EEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEE
DVTDEFADDAVEFKAFKGVIKSGGVVRAIPATGAAGQPRSFFDKLNDWARSEGAPGLGYI
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
VFEEEGGALTGKGPIAKFIPAAIQARIAEKAGAKAGDAVFFAAGTEAKAAGLAGKARIRI
EEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHCCCCCCEEEEE
GDELKLSDTDQFAFCWVVDFPMYEWNEEDKKIDFSHNPFSMPNFDRDEFLALGEADSEKI
CCEEEECCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHEEEECCCCCCCE
LGIKAFQYDIVCNGIELSSGAIRNHRPDVMEKAFAIAGYGRDVLEEKFGGMLNALRLGAP
EEEEEEEEEEEECCEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
PHGGIAPGVDRIVMLLCEEPNIREVVLFPMNQRAEDLMMGAPAEATPKQLRELHIRLNLP
CCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHEEEEEECCC
EKKA
CCCC
>Mature Secondary Structure
MHRYRTHTCGAIRPSDVGQTVRLSGWCHRIRDHGGVLFIDLRDHYGLTQCVIDSDSKAFK
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHH
AAETARSEWVIRIDGRVRTRPAGTENAELPTGSVEVYIDDLEVLGPAGELPLPVFGDQEY
HHHHCCCCEEEEECCEEEECCCCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCCCCCC
PEETRLKYRFLDLRREKLHANIMKRGAIVDSLRRRMREGGFFEFQTPILTASSPEGARDY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCCCCCC
LVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVFQAVEPVLRGVFEEFAGGKRVTKEFPRITYADAMLKYGVDKPDLRNPLIIA
EEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEE
DVTDEFADDAVEFKAFKGVIKSGGVVRAIPATGAAGQPRSFFDKLNDWARSEGAPGLGYI
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
VFEEEGGALTGKGPIAKFIPAAIQARIAEKAGAKAGDAVFFAAGTEAKAAGLAGKARIRI
EEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHCCCCCCEEEEE
GDELKLSDTDQFAFCWVVDFPMYEWNEEDKKIDFSHNPFSMPNFDRDEFLALGEADSEKI
CCEEEECCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHEEEECCCCCCCE
LGIKAFQYDIVCNGIELSSGAIRNHRPDVMEKAFAIAGYGRDVLEEKFGGMLNALRLGAP
EEEEEEEEEEEECCEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
PHGGIAPGVDRIVMLLCEEPNIREVVLFPMNQRAEDLMMGAPAEATPKQLRELHIRLNLP
CCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHEEEEEECCC
EKKA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA