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The map label for this gene is pleD [H]

Identifier: 218532240

GI number: 218532240

Start: 4596489

End: 4597598

Strand: Reverse

Name: pleD [H]

Synonym: Mchl_4352

Alternate gene names: 218532240

Gene position: 4597598-4596489 (Counterclockwise)

Preceding gene: 218532241

Following gene: 218532238

Centisome position: 79.57

GC content: 67.3

Gene sequence:

>1110_bases
ATGTGGGCGGCGTGCCCCGTCCGCAGGAGCCTACGTCACCGGATGAGCCCCGATCCCGATCGTGATCGCAGTTTCGTGCT
CGCGCAGCGCAGCTTCGAGCTGATGCGCGACTACTGTGCCTGCGCGACGCCGCGGGCCTACGCTGTGTGGTATCTCTACG
TCTCCGGCACGCAGCCGCTGATGAACGATGCGGTCAAACGGCTCACGACGCAGAACGGCACGCTGACCAGCGCCGACATC
GAGGGGCTCCACGACACCTACATCGACGGCCGCCGCATGGCCGTGGAGGTGGACCGGATGAATTCGAGCCTCATCGCCGA
GGTCGAGGGCATCATGGAAATGATCGAGGTGTCGATCCGCTCCACCGCCCAGTACGGCGAGTCGCTTCAGGCCCTGTCCC
ACGACATCGCCAACACGGCCACGAGCCGGGCGCGTCTGCGCGAGGTCGTGACGACGATCATCGCCAACACCCGCGACGTG
ACCGCCAACAACCGCACCCTCGAAGCGCGGATGCGCGAGAGCCGCAGCGAGATCGAGGCCTTACGCGAGACGCTGGAGGC
GACCCGTCTGGAGAGCCTGACCGACGCCCTGACGGGGCTGGGCAACCGCAAGAGTTTCGAGGAGTCGCTCCGCAAGAGCG
TCGATCTGCAGTCTGCCTCGGCGCAGCAGGGCAAGCCGGCGAGCCTGATCGTGATCGACATTGATTCGTTCAAGCGCTTC
AACGACCTCTACGGCCACCTGACGGGGGATCAGGTGCTGCGCCTCGTCGCCATCGTGATGCGCGAGCATATCGGTCAGAG
AAACGCGACGCTCGCCCGGTTCGGCGGCGAGGAATTCGGCATCGTCCTGCCGGAAACCGACCGGGTCGAGGCGCGCGCCA
TCGCCGAGCGGGTGCGCATCGGCGTCACCGGCCGCGAACTGGTGAAGCGCTCCACCGGCGAATCGCTCGGCAAGGTCACG
GTCTCGCTCGGCGTCGCGACCCAGCGCGAGGACGACAACGCGGTCTCCCTGCTGGAACGAGCCGACGCCTGCCTGTTCGC
CGCCAAGCGCGCCGGCCGCAACCGCACCCTCGACGAGACCGAAGTCGAGAACCTGCGCGACGTCGCCTGA

Upstream 100 bases:

>100_bases
TCAAAACGCCCGCGCGGTGAATGGCCAGCGCTCGGGCGCTTTGTGAGACGCTCTTAACGTCCTGGCGAGATTCTGAGACG
ACACTTCGGCGGAGCGGACG

Downstream 100 bases:

>100_bases
GACGGAAGAGAGCCTCGTCCCGAAAGGCGGCCTCCGGGACGAGGCGAGAGCCATGCGCTCAAGCCTCGTCCGGTCTGCGA
GACGAGGCAGCGCCGATCAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MWAACPVRRSLRHRMSPDPDRDRSFVLAQRSFELMRDYCACATPRAYAVWYLYVSGTQPLMNDAVKRLTTQNGTLTSADI
EGLHDTYIDGRRMAVEVDRMNSSLIAEVEGIMEMIEVSIRSTAQYGESLQALSHDIANTATSRARLREVVTTIIANTRDV
TANNRTLEARMRESRSEIEALRETLEATRLESLTDALTGLGNRKSFEESLRKSVDLQSASAQQGKPASLIVIDIDSFKRF
NDLYGHLTGDQVLRLVAIVMREHIGQRNATLARFGGEEFGIVLPETDRVEARAIAERVRIGVTGRELVKRSTGESLGKVT
VSLGVATQREDDNAVSLLERADACLFAAKRAGRNRTLDETEVENLRDVA

Sequences:

>Translated_369_residues
MWAACPVRRSLRHRMSPDPDRDRSFVLAQRSFELMRDYCACATPRAYAVWYLYVSGTQPLMNDAVKRLTTQNGTLTSADI
EGLHDTYIDGRRMAVEVDRMNSSLIAEVEGIMEMIEVSIRSTAQYGESLQALSHDIANTATSRARLREVVTTIIANTRDV
TANNRTLEARMRESRSEIEALRETLEATRLESLTDALTGLGNRKSFEESLRKSVDLQSASAQQGKPASLIVIDIDSFKRF
NDLYGHLTGDQVLRLVAIVMREHIGQRNATLARFGGEEFGIVLPETDRVEARAIAERVRIGVTGRELVKRSTGESLGKVT
VSLGVATQREDDNAVSLLERADACLFAAKRAGRNRTLDETEVENLRDVA
>Mature_369_residues
MWAACPVRRSLRHRMSPDPDRDRSFVLAQRSFELMRDYCACATPRAYAVWYLYVSGTQPLMNDAVKRLTTQNGTLTSADI
EGLHDTYIDGRRMAVEVDRMNSSLIAEVEGIMEMIEVSIRSTAQYGESLQALSHDIANTATSRARLREVVTTIIANTRDV
TANNRTLEARMRESRSEIEALRETLEATRLESLTDALTGLGNRKSFEESLRKSVDLQSASAQQGKPASLIVIDIDSFKRF
NDLYGHLTGDQVLRLVAIVMREHIGQRNATLARFGGEEFGIVLPETDRVEARAIAERVRIGVTGRELVKRSTGESLGKVT
VSLGVATQREDDNAVSLLERADACLFAAKRAGRNRTLDETEVENLRDVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=40.2366863905325, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693134, Length=207, Percent_Identity=35.2657004830918, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=38.0368098159509, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=32.972972972973, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=164, Percent_Identity=37.1951219512195, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082007, Length=190, Percent_Identity=33.6842105263158, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=34.3373493975904, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=182, Percent_Identity=33.5164835164835, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=191, Percent_Identity=29.3193717277487, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=220, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081977, Length=189, Percent_Identity=30.6878306878307, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=30.6451612903226, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=31.4814814814815, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081974, Length=170, Percent_Identity=28.8235294117647, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41123; Mature: 41123

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWAACPVRRSLRHRMSPDPDRDRSFVLAQRSFELMRDYCACATPRAYAVWYLYVSGTQPL
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHH
MNDAVKRLTTQNGTLTSADIEGLHDTYIDGRRMAVEVDRMNSSLIAEVEGIMEMIEVSIR
HHHHHHHHHCCCCCEEHHHHCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
STAQYGESLQALSHDIANTATSRARLREVVTTIIANTRDVTANNRTLEARMRESRSEIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LRETLEATRLESLTDALTGLGNRKSFEESLRKSVDLQSASAQQGKPASLIVIDIDSFKRF
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHH
NDLYGHLTGDQVLRLVAIVMREHIGQRNATLARFGGEEFGIVLPETDRVEARAIAERVRI
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHC
GVTGRELVKRSTGESLGKVTVSLGVATQREDDNAVSLLERADACLFAAKRAGRNRTLDET
CCCHHHHHHHHCCCHHHHEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EVENLRDVA
HHHHHHCCC
>Mature Secondary Structure
MWAACPVRRSLRHRMSPDPDRDRSFVLAQRSFELMRDYCACATPRAYAVWYLYVSGTQPL
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHH
MNDAVKRLTTQNGTLTSADIEGLHDTYIDGRRMAVEVDRMNSSLIAEVEGIMEMIEVSIR
HHHHHHHHHCCCCCEEHHHHCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
STAQYGESLQALSHDIANTATSRARLREVVTTIIANTRDVTANNRTLEARMRESRSEIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LRETLEATRLESLTDALTGLGNRKSFEESLRKSVDLQSASAQQGKPASLIVIDIDSFKRF
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHH
NDLYGHLTGDQVLRLVAIVMREHIGQRNATLARFGGEEFGIVLPETDRVEARAIAERVRI
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHC
GVTGRELVKRSTGESLGKVTVSLGVATQREDDNAVSLLERADACLFAAKRAGRNRTLDET
CCCHHHHHHHHCCCHHHHEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EVENLRDVA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA