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The map label for this gene is cspE [C]

Identifier: 218532203

GI number: 218532203

Start: 4554862

End: 4555071

Strand: Reverse

Name: cspE [C]

Synonym: Mchl_4315

Alternate gene names: 218532203

Gene position: 4555071-4554862 (Counterclockwise)

Preceding gene: 218532204

Following gene: 218532202

Centisome position: 78.84

GC content: 65.24

Gene sequence:

>210_bases
ATGCAGACTGGTACCGTGAAATGGTTCGATGAGGTCAAGGGCTACGGGTTCATCCAGCCCGATACCGGGGGCAAGGACGT
GTTCGTGCATATCTCCGCCGTCCAGCAGGCGGGCCTGCGCGGCCTCGTCGAGGGGCAGAAGGTCAGCTTCGACGTCGAGA
ACGACCGCCGCAGCGGCAAGCCGGCCGCGGTCAACCTGCAGGCCAGCTGA

Upstream 100 bases:

>100_bases
GCTTGTTTCCTCGCTCGCTTCGACGAACGGGACGGCCTTTTCGGCCGAGGACGGGACGCATTTCATACGGGGTGCCGCGG
CGAGCGGAGGAGGAACCCGG

Downstream 100 bases:

>100_bases
GGCCGGATACGCTGGACGCGAGATACACTGGACGCTTGAGGGCCCTGCCCTAAAACCTGATTCCATCCCGCAACAGGTCC
AAAAATGTCACCGGAGCCGC

Product: cold-shock DNA-binding domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 69

Protein sequence:

>69_residues
MQTGTVKWFDEVKGYGFIQPDTGGKDVFVHISAVQQAGLRGLVEGQKVSFDVENDRRSGKPAAVNLQAS

Sequences:

>Translated_69_residues
MQTGTVKWFDEVKGYGFIQPDTGGKDVFVHISAVQQAGLRGLVEGQKVSFDVENDRRSGKPAAVNLQAS
>Mature_69_residues
MQTGTVKWFDEVKGYGFIQPDTGGKDVFVHISAVQQAGLRGLVEGQKVSFDVENDRRSGKPAAVNLQAS

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=52.3076923076923, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=55.2238805970149, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=53.8461538461538, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=54.6875, Blast_Score=72, Evalue=7e-15,
Organism=Escherichia coli, GI1787839, Length=65, Percent_Identity=53.8461538461538, Blast_Score=72, Evalue=9e-15,
Organism=Escherichia coli, GI1787834, Length=66, Percent_Identity=51.5151515151515, Blast_Score=69, Evalue=5e-14,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=45.3125, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7466; Mature: 7466

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTGTVKWFDEVKGYGFIQPDTGGKDVFVHISAVQQAGLRGLVEGQKVSFDVENDRRSGK
CCCCCEEEHHCCCCCEEECCCCCCCEEEEEEEHHHCCCCCCCCCCCEEEEEECCCCCCCC
PAAVNLQAS
CEEEEEECC
>Mature Secondary Structure
MQTGTVKWFDEVKGYGFIQPDTGGKDVFVHISAVQQAGLRGLVEGQKVSFDVENDRRSGK
CCCCCEEEHHCCCCCEEECCCCCCCEEEEEEEHHHCCCCCCCCCCCEEEEEECCCCCCCC
PAAVNLQAS
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]