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The map label for this gene is mdtE [H]

Identifier: 218532070

GI number: 218532070

Start: 4387869

End: 4389185

Strand: Reverse

Name: mdtE [H]

Synonym: Mchl_4164

Alternate gene names: 218532070

Gene position: 4389185-4387869 (Counterclockwise)

Preceding gene: 218532071

Following gene: 218532069

Centisome position: 75.96

GC content: 71.98

Gene sequence:

>1317_bases
ATGTCTGATGACGCCCGTCCGATCCGACCGGAGGATTCGCCCGTGACCGACGGCGAGGACAAGAAAATCCCGCCGCCGCC
GGGCAAGGGCGGCTTCGTGTGGGCCGGGCTGATCGCGCTCGGCCTGCTGGCCTATGGCGGCTACGGCCACTGGCAGCGGG
CGGCCCGGGCCGAGTCGACGCGGGCCCGGCAGATGGAGTTCGTGCCGAAAGTGCGCACCGAGGAGGCCAAGCGCCTCGAC
GGGCCGATCGAGCTGACCCTGCCCGGCCAGACCGAGCCCTTCGCCACCGCGCGGATCTATCCCCGCGCCACCGGCTACAT
CGCCGAGCGCCATGTCGATCTCGGCTCGCGGGTGAAGAAGGGCGACGTGCTCCTGCGCATCGCCGCCCCGGATCTCGACC
AGCAACTCGCGCAAGCCGAGGCGCAGCTCGGGCAGCTCGAGGCCCAGCTCCTGCGGGCCCGGGCCATGGTCGATCAGGCC
AAGGCCAACACGAACCTCGCCCAGGCCACCAACTCGCGCACCTCGACGCTCGCCGGCCAGGGCTGGGCCTCGAAGCAGAA
CGCCGACAACACCCAGGCCGGCGTGCTGGCGCAGCAGGCGAACCTTGCCTCGGCGGAGGCCGACGTGAAGGTGGCCCAGG
CCAATATCAAGGCGCAGCAGGCGACCGTGGGGCGGCTCAAGGCGCTGACCGGCTTCGAGGTCGTCACCGCCCCCTTCGAC
GGCGTGATCACCACCCGCAACGTCGATGTCGGCGACCTCGTCCAGGCCGATGCCGGCTCCGGCTCGCCGCTCCTGTCGAT
GGACCGGGACGACGTGCTGCGCATCTCGGTCAACGTGCCGCAGAACGCGGCGGTGGGCGTGCAGCCCGGCATCGCGGCCC
AGATCAAGGTGCCGCAGATGCCCGACCGCAGCTTCGCCGGCGTGGTCGAGCGCAGTTCGGTGGCCCTGCTCGCCTCCTCG
CGCACCCTGACGACGCAGGTCGACGTGCCGAACCCCGACCTCACCCTGCGGCCGGGCCTCTACGTCTACGTGACCCTGGC
GATCCCCCGCACCGGCGGCGCCACGGTGGCGGTGCCGGCCGAGGCCCTGGTGTTCAACCAGTCCGGCACCCGCGTGGCCG
TCGCCAGCGAGGACGACCGCGTGCGTTGGGTCGAGGTCCATGTCGCCCGCGACAAGGGCACGGTGGTCGAGGTGGACAAG
GGGCTCTCGGGCGGCGAGCGCCTCGTGCTGAGCCCCCCGGCCGATCTCAAGGACGGCGGCCGCATCGCCCCCCAGGCGCC
GAAATCGGACAAGCCGATGCAAGCCGCGCAGCGCTAA

Upstream 100 bases:

>100_bases
GTGATGGGCGGCATCGCGCTCGGCACGCCCTCGACCCTGCTGTTCGTGCCGTTCCTCTACAGCCTGCTGCGGCGGGGCGA
GGCCAAGCCGCTCGAGGATT

Downstream 100 bases:

>100_bases
GACTTTCGCGCAGCGCTAAGACTTTCGCGCGGCGCTGATCCCATCCTCGTCCTCATCCTGAGGTGCCGGAGCGAAGCGGA
GGCCTCGAAGGATCCTCCAG

Product: efflux transporter RND family, MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSDDARPIRPEDSPVTDGEDKKIPPPPGKGGFVWAGLIALGLLAYGGYGHWQRAARAESTRARQMEFVPKVRTEEAKRLD
GPIELTLPGQTEPFATARIYPRATGYIAERHVDLGSRVKKGDVLLRIAAPDLDQQLAQAEAQLGQLEAQLLRARAMVDQA
KANTNLAQATNSRTSTLAGQGWASKQNADNTQAGVLAQQANLASAEADVKVAQANIKAQQATVGRLKALTGFEVVTAPFD
GVITTRNVDVGDLVQADAGSGSPLLSMDRDDVLRISVNVPQNAAVGVQPGIAAQIKVPQMPDRSFAGVVERSSVALLASS
RTLTTQVDVPNPDLTLRPGLYVYVTLAIPRTGGATVAVPAEALVFNQSGTRVAVASEDDRVRWVEVHVARDKGTVVEVDK
GLSGGERLVLSPPADLKDGGRIAPQAPKSDKPMQAAQR

Sequences:

>Translated_438_residues
MSDDARPIRPEDSPVTDGEDKKIPPPPGKGGFVWAGLIALGLLAYGGYGHWQRAARAESTRARQMEFVPKVRTEEAKRLD
GPIELTLPGQTEPFATARIYPRATGYIAERHVDLGSRVKKGDVLLRIAAPDLDQQLAQAEAQLGQLEAQLLRARAMVDQA
KANTNLAQATNSRTSTLAGQGWASKQNADNTQAGVLAQQANLASAEADVKVAQANIKAQQATVGRLKALTGFEVVTAPFD
GVITTRNVDVGDLVQADAGSGSPLLSMDRDDVLRISVNVPQNAAVGVQPGIAAQIKVPQMPDRSFAGVVERSSVALLASS
RTLTTQVDVPNPDLTLRPGLYVYVTLAIPRTGGATVAVPAEALVFNQSGTRVAVASEDDRVRWVEVHVARDKGTVVEVDK
GLSGGERLVLSPPADLKDGGRIAPQAPKSDKPMQAAQR
>Mature_437_residues
SDDARPIRPEDSPVTDGEDKKIPPPPGKGGFVWAGLIALGLLAYGGYGHWQRAARAESTRARQMEFVPKVRTEEAKRLDG
PIELTLPGQTEPFATARIYPRATGYIAERHVDLGSRVKKGDVLLRIAAPDLDQQLAQAEAQLGQLEAQLLRARAMVDQAK
ANTNLAQATNSRTSTLAGQGWASKQNADNTQAGVLAQQANLASAEADVKVAQANIKAQQATVGRLKALTGFEVVTAPFDG
VITTRNVDVGDLVQADAGSGSPLLSMDRDDVLRISVNVPQNAAVGVQPGIAAQIKVPQMPDRSFAGVVERSSVALLASSR
TLTTQVDVPNPDLTLRPGLYVYVTLAIPRTGGATVAVPAEALVFNQSGTRVAVASEDDRVRWVEVHVARDKGTVVEVDKG
LSGGERLVLSPPADLKDGGRIAPQAPKSDKPMQAAQR

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=337, Percent_Identity=27.299703264095, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786668, Length=374, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081796, Length=330, Percent_Identity=25.1515151515152, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082036, Length=347, Percent_Identity=25.3602305475504, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 46392; Mature: 46261

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDARPIRPEDSPVTDGEDKKIPPPPGKGGFVWAGLIALGLLAYGGYGHWQRAARAEST
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RARQMEFVPKVRTEEAKRLDGPIELTLPGQTEPFATARIYPRATGYIAERHVDLGSRVKK
HHHHHHHCCCCCCHHHHHCCCCEEEEECCCCCCCEEEEECCCCCCEEHHCCCCCCCCCCC
GDVLLRIAAPDLDQQLAQAEAQLGQLEAQLLRARAMVDQAKANTNLAQATNSRTSTLAGQ
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEECCC
GWASKQNADNTQAGVLAQQANLASAEADVKVAQANIKAQQATVGRLKALTGFEVVTAPFD
CCCCCCCCCCCHHHEEEHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCCC
GVITTRNVDVGDLVQADAGSGSPLLSMDRDDVLRISVNVPQNAAVGVQPGIAAQIKVPQM
CEEEECCCCHHHHEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEECCCC
PDRSFAGVVERSSVALLASSRTLTTQVDVPNPDLTLRPGLYVYVTLAIPRTGGATVAVPA
CCCHHHHHHCCCCEEEEECCCEEEEEEECCCCCEEECCCEEEEEEEEEECCCCCEEEECC
EALVFNQSGTRVAVASEDDRVRWVEVHVARDKGTVVEVDKGLSGGERLVLSPPADLKDGG
EEEEECCCCCEEEEECCCCCEEEEEEEEECCCCCEEEECCCCCCCCEEEECCCCCCCCCC
RIAPQAPKSDKPMQAAQR
EECCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SDDARPIRPEDSPVTDGEDKKIPPPPGKGGFVWAGLIALGLLAYGGYGHWQRAARAEST
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RARQMEFVPKVRTEEAKRLDGPIELTLPGQTEPFATARIYPRATGYIAERHVDLGSRVKK
HHHHHHHCCCCCCHHHHHCCCCEEEEECCCCCCCEEEEECCCCCCEEHHCCCCCCCCCCC
GDVLLRIAAPDLDQQLAQAEAQLGQLEAQLLRARAMVDQAKANTNLAQATNSRTSTLAGQ
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEECCC
GWASKQNADNTQAGVLAQQANLASAEADVKVAQANIKAQQATVGRLKALTGFEVVTAPFD
CCCCCCCCCCCHHHEEEHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCCC
GVITTRNVDVGDLVQADAGSGSPLLSMDRDDVLRISVNVPQNAAVGVQPGIAAQIKVPQM
CEEEECCCCHHHHEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEECCCC
PDRSFAGVVERSSVALLASSRTLTTQVDVPNPDLTLRPGLYVYVTLAIPRTGGATVAVPA
CCCHHHHHHCCCCEEEEECCCEEEEEEECCCCCEEECCCEEEEEEEEEECCCCCEEEECC
EALVFNQSGTRVAVASEDDRVRWVEVHVARDKGTVVEVDKGLSGGERLVLSPPADLKDGG
EEEEECCCCCEEEEECCCCCEEEEEEEEECCCCCEEEECCCCCCCCEEEECCCCCCCCCC
RIAPQAPKSDKPMQAAQR
EECCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]