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The map label for this gene is rnhA [H]

Identifier: 218531996

GI number: 218531996

Start: 4303595

End: 4304056

Strand: Reverse

Name: rnhA [H]

Synonym: Mchl_4085

Alternate gene names: 218531996

Gene position: 4304056-4303595 (Counterclockwise)

Preceding gene: 218531997

Following gene: 218531995

Centisome position: 74.49

GC content: 64.07

Gene sequence:

>462_bases
ATGTCGGAAGAACCTACTAGAGAAATCACCATTGCCTTCGACGGCGCATGTCGCGGCAACCCTGGCCCAGGCGGCTATGG
GGCCATCCTCATCAACACCCGCACGGGCGCTGAGAAGATCGTGAAGGGGCGCGAGGTAGCCACGACCAACAACCGCATGG
AGCTGTCAGCGGCCATCGCCGGGCTGAACGCCATACGCCCTGGCGCCATCGTCGCGATGATCGGAGATAGCCAGTACGTT
ATCAAGGGCATCACCGAGTGGCTCCCCGGCTGGAAGGCCAGGGGCTGGAGCGGAGCGGGTGGAAAGCCCGTCTTGAACAA
AGAGCTATGGCAGTCGCTGGAGATGGCCGTAGCGCGGCACCTGACGGTAACGTGGACGTGGGTGCGAGGGCACGCGGGGC
ATGAGCTGAACGAGCGCGTGGACGAGATCGCAGCGGCTCAAGCGGGGCTGGCTCATGGGTGA

Upstream 100 bases:

>100_bases
CACGCGCCTACCCAGCGCACTGAGCAGACCCACACACCATCAACGACCACCCGGAGGGGCCTCGAAAGGGGCTCCTCTTT
TTCATTCAAGGAGACCGACG

Downstream 100 bases:

>100_bases
GCCGGGTCGCAAACTGGGCTCCCCACAAGCCTTGCCGCCTCACGACACGATCACTGAGGGGGACGCGATAGGCGTCCTCC
TGTCCAACCGACCGGCACGG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MSEEPTREITIAFDGACRGNPGPGGYGAILINTRTGAEKIVKGREVATTNNRMELSAAIAGLNAIRPGAIVAMIGDSQYV
IKGITEWLPGWKARGWSGAGGKPVLNKELWQSLEMAVARHLTVTWTWVRGHAGHELNERVDEIAAAQAGLAHG

Sequences:

>Translated_153_residues
MSEEPTREITIAFDGACRGNPGPGGYGAILINTRTGAEKIVKGREVATTNNRMELSAAIAGLNAIRPGAIVAMIGDSQYV
IKGITEWLPGWKARGWSGAGGKPVLNKELWQSLEMAVARHLTVTWTWVRGHAGHELNERVDEIAAAQAGLAHG
>Mature_152_residues
SEEPTREITIAFDGACRGNPGPGGYGAILINTRTGAEKIVKGREVATTNNRMELSAAIAGLNAIRPGAIVAMIGDSQYVI
KGITEWLPGWKARGWSGAGGKPVLNKELWQSLEMAVARHLTVTWTWVRGHAGHELNERVDEIAAAQAGLAHG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=98, Percent_Identity=36.734693877551, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1786408, Length=142, Percent_Identity=52.112676056338, Blast_Score=145, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45552509, Length=149, Percent_Identity=34.2281879194631, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17137476, Length=149, Percent_Identity=34.2281879194631, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16296; Mature: 16165

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEPTREITIAFDGACRGNPGPGGYGAILINTRTGAEKIVKGREVATTNNRMELSAAIA
CCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHCCCEEEECCCCEEHHHHHH
GLNAIRPGAIVAMIGDSQYVIKGITEWLPGWKARGWSGAGGKPVLNKELWQSLEMAVARH
CCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LTVTWTWVRGHAGHELNERVDEIAAAQAGLAHG
EEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEEPTREITIAFDGACRGNPGPGGYGAILINTRTGAEKIVKGREVATTNNRMELSAAIA
CCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHCCCEEEECCCCEEHHHHHH
GLNAIRPGAIVAMIGDSQYVIKGITEWLPGWKARGWSGAGGKPVLNKELWQSLEMAVARH
CCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LTVTWTWVRGHAGHELNERVDEIAAAQAGLAHG
EEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA