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The map label for this gene is stcD [H]

Identifier: 218531953

GI number: 218531953

Start: 4255225

End: 4257267

Strand: Reverse

Name: stcD [H]

Synonym: Mchl_4041

Alternate gene names: 218531953

Gene position: 4257267-4255225 (Counterclockwise)

Preceding gene: 218531954

Following gene: 218531952

Centisome position: 73.68

GC content: 69.6

Gene sequence:

>2043_bases
GTGATCGCCAATGCCGACGCGCTCTTGAAGCCCCTCACCATCAAGGGCCTCACCATCCGCAACCGGGTGATGTCCACGAG
CCACGCCCCCGGCTACGGCCGGGACGGCAAGCCGCAGGAGCGCTACCAGCTCTACCACGCCGAGAAGGCCAAGGGCGGCA
TCGGGCTCACCATGTTCGGCGGCTCGTCCTCGGTCGCCGTCGACAGCCCCGCCGCACCCTGGAACCAGATCTCGGTCGCC
GACGACTCAGTGATCCCGTTCTTCCAGCAGTTCTCGGACCGGGTGCACGCGCATGGCGGCAAGCTGATGATTCAGCTCAC
CCATATGGGCCGGCGCACCAAGTGGGACACCGAGCACTGGCTCCCCACCCTCTCGCCCTCCCCCCGGCGTGAGCCCGCCT
CCCGCACCATCCCGAAGGAGATGGAAGCGGAGGACATCGCCCGCATCGTCAAGAGCTTCGCCCAGGCCGCGCGCCGTTGC
CGCGAGGGCGGGCTCGACGGCTGCGAGGTCTCGGCGGCCCACGGCCACCTGATCGACCAGTTCTGGTCGCCGTCGGTCAA
CCAGCGCACCGACCAATACGGCGGCAGCCTGGAGAACCGGATGCGCTTCGGCATCGAGGTGCTGGAGGCGATGCGCGCGG
AAGCGGGTGACGACTACGTCATCGGCATCCGCATGTCCGGCGACGAGATGATCGCCGACGGCCTCAGCCAGGACGATTGC
GTCGCCATCGCCTCCGAATATGCCAAGCGCGGGCTCGTGGACTTCCTCAACATCCTCGGCGGGCAAGCGCGCGACCACAT
CGCCCATGCGATCTCGCTGCCGAACATGTCCTTCCCCGTGGCGCCGTTCCTGTTTCTGCCGAGCGCGATCAAGCGCGAGG
TCGACGTGCCGGTCTTCCACGCCCAGCGCGTCACCGATCTGGCCACCGCCGCCCGCGCGGTCGCGGAGGGCCATGTCGAC
ATGGTGGCGATGACGCGCGCCCATATCGCCGACCCGCATCTCGTGAAGAAGCTCTCCGAGGGCCGGGCCGACGACATCCG
CCAATGCGTCGGTGCGGGCTACTGCATCGACCGGATCTATGTCGGCGGCGACGCGCTCTGCATCCAGAACGCCGCCACCG
GCCGCGAGGCGACGATGCCGCACGAGATCCGCAAGGCGGATCTGCGCCGCCGGGTCGTGGTGATCGGCGCCGGCCCCGGC
GGCCTCGAGGCCGCCCGCGTCTGCGCCGAGCGCGGCCACGCGGTGGTGCTGTTCGAGAAGAGCGGGCAGGCCGGCGGCCA
GATCGGCATCGCGGCACGCGCCGGCTGGCGCGAGGCGATGTCGGGCATCACCCGCTGGCTGGTGGCCCAGGTGACCAAGC
TCGGCGTCGATCTGCGCCTCAACACCGAGGCGACCGAGGCGGCGGTGCTCGCCGAGAAGCCGGACGTGATCATCGTCGCC
ACCGGCGGCACGCCGTTCCGCGGCCACGCCAAGGGCGCCGCGCAGCACGCGGTCACCACCGCCGAGGTTCTGGCCGGCGC
GGTCGAGCCCACCGGCTCGGTCCTGCTCTACGACGAGATGGGCCAGCACAACGCCGCCTCGGTGGCCGAGCAATTGGCCA
AGCGCGGCTGCCTCGTGGAGATCGCCACCCACGACCGCATGGTGGCCGAGGAGGTCGGCACCACCAACCAGCCGATCCAC
CTGCGCGAGCTCTACAAGCTCGGCGTAGTGATGAGCCCGAACCTGGAGCTGATCGAGATCTATCCGGAGGGCAACCGCTT
CGTCGCGGCGTTGCGCAACACCATGACCGACGCCGAGGAGGAGCGCCTCGTCGACCGCATCGTCGTCGATCACGGCACGC
TGCCGGTGGACAGGCTCTACCACGGCTTGAAGGAGGCCTCGGTCAACCGGGGCCAGACCGACCCGGACGCACTGATCCGC
GGCGAGCCGCAGCCCTACGACCTGTCGAAGGGCTACGCGCTCTATCGCATCGGCGACGCGGTCTCGGGCCGCAACATCCA
CGCGGCGATCTACGACGCCCTGCGGCTGTGCAAGGATCTTTGA

Upstream 100 bases:

>100_bases
GCTCGAAGCCGACCAGCGACCTCGTCATCGAACGCTGAACGGGCGGGCTCGGCCCCGCCCGCGACCCCACGCTTTCCCCA
TCGCCTTCGGGAGTCGTCCC

Downstream 100 bases:

>100_bases
TTGGGTTCCCCCTCTCCCCGCACGCGGGGAGAGGCCCGCAGGCACCCTGTCGTGCCTGCGGGAAGCGGAGGTGAAGCCGG
AGCGGCGGTGAGGGGGCGGC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MIANADALLKPLTIKGLTIRNRVMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFGGSSSVAVDSPAAPWNQISVA
DDSVIPFFQQFSDRVHAHGGKLMIQLTHMGRRTKWDTEHWLPTLSPSPRREPASRTIPKEMEAEDIARIVKSFAQAARRC
REGGLDGCEVSAAHGHLIDQFWSPSVNQRTDQYGGSLENRMRFGIEVLEAMRAEAGDDYVIGIRMSGDEMIADGLSQDDC
VAIASEYAKRGLVDFLNILGGQARDHIAHAISLPNMSFPVAPFLFLPSAIKREVDVPVFHAQRVTDLATAARAVAEGHVD
MVAMTRAHIADPHLVKKLSEGRADDIRQCVGAGYCIDRIYVGGDALCIQNAATGREATMPHEIRKADLRRRVVVIGAGPG
GLEAARVCAERGHAVVLFEKSGQAGGQIGIAARAGWREAMSGITRWLVAQVTKLGVDLRLNTEATEAAVLAEKPDVIIVA
TGGTPFRGHAKGAAQHAVTTAEVLAGAVEPTGSVLLYDEMGQHNAASVAEQLAKRGCLVEIATHDRMVAEEVGTTNQPIH
LRELYKLGVVMSPNLELIEIYPEGNRFVAALRNTMTDAEEERLVDRIVVDHGTLPVDRLYHGLKEASVNRGQTDPDALIR
GEPQPYDLSKGYALYRIGDAVSGRNIHAAIYDALRLCKDL

Sequences:

>Translated_680_residues
MIANADALLKPLTIKGLTIRNRVMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFGGSSSVAVDSPAAPWNQISVA
DDSVIPFFQQFSDRVHAHGGKLMIQLTHMGRRTKWDTEHWLPTLSPSPRREPASRTIPKEMEAEDIARIVKSFAQAARRC
REGGLDGCEVSAAHGHLIDQFWSPSVNQRTDQYGGSLENRMRFGIEVLEAMRAEAGDDYVIGIRMSGDEMIADGLSQDDC
VAIASEYAKRGLVDFLNILGGQARDHIAHAISLPNMSFPVAPFLFLPSAIKREVDVPVFHAQRVTDLATAARAVAEGHVD
MVAMTRAHIADPHLVKKLSEGRADDIRQCVGAGYCIDRIYVGGDALCIQNAATGREATMPHEIRKADLRRRVVVIGAGPG
GLEAARVCAERGHAVVLFEKSGQAGGQIGIAARAGWREAMSGITRWLVAQVTKLGVDLRLNTEATEAAVLAEKPDVIIVA
TGGTPFRGHAKGAAQHAVTTAEVLAGAVEPTGSVLLYDEMGQHNAASVAEQLAKRGCLVEIATHDRMVAEEVGTTNQPIH
LRELYKLGVVMSPNLELIEIYPEGNRFVAALRNTMTDAEEERLVDRIVVDHGTLPVDRLYHGLKEASVNRGQTDPDALIR
GEPQPYDLSKGYALYRIGDAVSGRNIHAAIYDALRLCKDL
>Mature_680_residues
MIANADALLKPLTIKGLTIRNRVMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFGGSSSVAVDSPAAPWNQISVA
DDSVIPFFQQFSDRVHAHGGKLMIQLTHMGRRTKWDTEHWLPTLSPSPRREPASRTIPKEMEAEDIARIVKSFAQAARRC
REGGLDGCEVSAAHGHLIDQFWSPSVNQRTDQYGGSLENRMRFGIEVLEAMRAEAGDDYVIGIRMSGDEMIADGLSQDDC
VAIASEYAKRGLVDFLNILGGQARDHIAHAISLPNMSFPVAPFLFLPSAIKREVDVPVFHAQRVTDLATAARAVAEGHVD
MVAMTRAHIADPHLVKKLSEGRADDIRQCVGAGYCIDRIYVGGDALCIQNAATGREATMPHEIRKADLRRRVVVIGAGPG
GLEAARVCAERGHAVVLFEKSGQAGGQIGIAARAGWREAMSGITRWLVAQVTKLGVDLRLNTEATEAAVLAEKPDVIIVA
TGGTPFRGHAKGAAQHAVTTAEVLAGAVEPTGSVLLYDEMGQHNAASVAEQLAKRGCLVEIATHDRMVAEEVGTTNQPIH
LRELYKLGVVMSPNLELIEIYPEGNRFVAALRNTMTDAEEERLVDRIVVDHGTLPVDRLYHGLKEASVNRGQTDPDALIR
GEPQPYDLSKGYALYRIGDAVSGRNIHAAIYDALRLCKDL

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=484, Percent_Identity=32.6446280991736, Blast_Score=246, Evalue=5e-66,
Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=27.7777777777778, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=268, Percent_Identity=31.3432835820896, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=335, Percent_Identity=29.2537313432836, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17540738, Length=400, Percent_Identity=27.25, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17564188, Length=339, Percent_Identity=26.5486725663717, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=269, Percent_Identity=30.4832713754647, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=268, Percent_Identity=27.9850746268657, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=206, Percent_Identity=26.6990291262136, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=83, Percent_Identity=44.578313253012, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=86, Percent_Identity=40.6976744186046, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 73725; Mature: 73725

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIANADALLKPLTIKGLTIRNRVMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFG
CCCCCHHHHCCEEEECEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
GSSSVAVDSPAAPWNQISVADDSVIPFFQQFSDRVHAHGGKLMIQLTHMGRRTKWDTEHW
CCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHC
LPTLSPSPRREPASRTIPKEMEAEDIARIVKSFAQAARRCREGGLDGCEVSAAHGHLIDQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHH
FWSPSVNQRTDQYGGSLENRMRFGIEVLEAMRAEAGDDYVIGIRMSGDEMIADGLSQDDC
HCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHCCCCCCHH
VAIASEYAKRGLVDFLNILGGQARDHIAHAISLPNMSFPVAPFLFLPSAIKREVDVPVFH
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHCCCCCHHH
AQRVTDLATAARAVAEGHVDMVAMTRAHIADPHLVKKLSEGRADDIRQCVGAGYCIDRIY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCEEHEEE
VGGDALCIQNAATGREATMPHEIRKADLRRRVVVIGAGPGGLEAARVCAERGHAVVLFEK
ECCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEC
SGQAGGQIGIAARAGWREAMSGITRWLVAQVTKLGVDLRLNTEATEAAVLAEKPDVIIVA
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHEEECCCCEEEEE
TGGTPFRGHAKGAAQHAVTTAEVLAGAVEPTGSVLLYDEMGQHNAASVAEQLAKRGCLVE
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEE
IATHDRMVAEEVGTTNQPIHLRELYKLGVVMSPNLELIEIYPEGNRFVAALRNTMTDAEE
EECCCHHHHHHCCCCCCCCHHHHHHHCCCEECCCCEEEEEEECCCHHHHHHHHHHCCCHH
ERLVDRIVVDHGTLPVDRLYHGLKEASVNRGQTDPDALIRGEPQPYDLSKGYALYRIGDA
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCC
VSGRNIHAAIYDALRLCKDL
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIANADALLKPLTIKGLTIRNRVMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFG
CCCCCHHHHCCEEEECEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
GSSSVAVDSPAAPWNQISVADDSVIPFFQQFSDRVHAHGGKLMIQLTHMGRRTKWDTEHW
CCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHC
LPTLSPSPRREPASRTIPKEMEAEDIARIVKSFAQAARRCREGGLDGCEVSAAHGHLIDQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHH
FWSPSVNQRTDQYGGSLENRMRFGIEVLEAMRAEAGDDYVIGIRMSGDEMIADGLSQDDC
HCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHCCCCCCHH
VAIASEYAKRGLVDFLNILGGQARDHIAHAISLPNMSFPVAPFLFLPSAIKREVDVPVFH
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHCCCCCHHH
AQRVTDLATAARAVAEGHVDMVAMTRAHIADPHLVKKLSEGRADDIRQCVGAGYCIDRIY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCEEHEEE
VGGDALCIQNAATGREATMPHEIRKADLRRRVVVIGAGPGGLEAARVCAERGHAVVLFEK
ECCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEC
SGQAGGQIGIAARAGWREAMSGITRWLVAQVTKLGVDLRLNTEATEAAVLAEKPDVIIVA
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHEEECCCCEEEEE
TGGTPFRGHAKGAAQHAVTTAEVLAGAVEPTGSVLLYDEMGQHNAASVAEQLAKRGCLVE
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEE
IATHDRMVAEEVGTTNQPIHLRELYKLGVVMSPNLELIEIYPEGNRFVAALRNTMTDAEE
EECCCHHHHHHCCCCCCCCHHHHHHHCCCEECCCCEEEEEEECCCHHHHHHHHHHCCCHH
ERLVDRIVVDHGTLPVDRLYHGLKEASVNRGQTDPDALIRGEPQPYDLSKGYALYRIGDA
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCC
VSGRNIHAAIYDALRLCKDL
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]