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The map label for this gene is rffG [H]

Identifier: 218531919

GI number: 218531919

Start: 4213543

End: 4214601

Strand: Reverse

Name: rffG [H]

Synonym: Mchl_4003

Alternate gene names: 218531919

Gene position: 4214601-4213543 (Counterclockwise)

Preceding gene: 218531920

Following gene: 218531918

Centisome position: 72.94

GC content: 68.84

Gene sequence:

>1059_bases
ATGCGCATTCTGGTAACGGGCGGCTGCGGCTTCATCGGCTCCGCCCTCGTGCTGCATCTGGTGCAGGATCTCGGCCACGA
GGTGCTCACCCTCGACGCCATGACCTACGCCGCCAACCCGATCTCGCTGCAGCCGCTGGCGGACGATCCGCGCCACCGCC
TGGAACAGGCCGACATCTGCGATCCGGCCCGCGTCCACGCCCTCTACGCCGAGTTCAGGCCCGAGGCGGTGATGCATCTG
GCCGCCGAGAGCCATGTCGATCGCTCGATCACCGATCCGGGTGCCTTCGTGCGCACCAACGTGATCGGCACCCAGGTCAT
GCTCGACGGCGCCCGCACCCATTGGGAGAGCCTCGACCGCGAAGCCAAGGCGACGTTCCGCTTCCTCCACGTCTCGACCG
ACGAGGTCTACGGCTCGCTGCCGCCGGACGCGTTCTTCACCGAGGAGAGCCGCTACGATCCGCGCTCGCCCTACTCGGCG
TCAAAGGCGGCCTCCGACCATCTCGCCCGCGCCTGGCACGAGACCTACGGCCTGCCGGTGCTGGTGACGAACTGCTCGAA
CAATTACGGCCCGCGCCACTTCCCGGAAAAGCTGATCCCACTGATGATCCTGGCTGCGCTGGAGGGCAAGCCGCTCCCGG
TCTACGGTGACGGCCTGAACGAGCGCGACTGGATCCATGTCGAGGACCATGCCCGCGGCCTCGTCGCGGTGCTGGAGCGC
GGCCGGCTCGGCGAGACCTACCTGCTGGGCGGGCGCTCGGTGCGGAATAACCTCGCCGTGGTGAAGGCGCTCTGCGCCGC
CTTCGACCAACTCAAGCCCGAGAACGGCCCGCACGAACGCCTGATCAGCTTCGTCGCCGACCGGCCCGGCCATGACCGGC
GCTACGCGATCGACCCGTCGAAGGCGGAAGCCGAGGTCGGCTGGCGGCCGACCAAGGTGTTCGAGGAGGCGCTGACGGAA
ACCGTGCGCTGGTATCTCGACAACGAGGCGTGGTGGCGCCCGATCCGCGAGGGCCGCTATTCCGGCGAGCGCCTCGGCCT
CGCGCCCAAGAGCGCCTGA

Upstream 100 bases:

>100_bases
TGAGCCCGACCTCAGGTTGGCGTAGGCGCGGCCGAGCGTGGCCCTTGCCCATCCCGCGGATGCCGACCCCGCGGATAGGA
ATTGGACGGAGTTGGGATCG

Downstream 100 bases:

>100_bases
GCATGGACATCCTGATCCTCGGCGGCGCCGGCCAGGTCGGCACGGAGCTTCAGGCCTTGTCCTGGCCCGAGGGCGTGCGG
GTGCACGCACCCGACCGCCA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MRILVTGGCGFIGSALVLHLVQDLGHEVLTLDAMTYAANPISLQPLADDPRHRLEQADICDPARVHALYAEFRPEAVMHL
AAESHVDRSITDPGAFVRTNVIGTQVMLDGARTHWESLDREAKATFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSA
SKAASDHLARAWHETYGLPVLVTNCSNNYGPRHFPEKLIPLMILAALEGKPLPVYGDGLNERDWIHVEDHARGLVAVLER
GRLGETYLLGGRSVRNNLAVVKALCAAFDQLKPENGPHERLISFVADRPGHDRRYAIDPSKAEAEVGWRPTKVFEEALTE
TVRWYLDNEAWWRPIREGRYSGERLGLAPKSA

Sequences:

>Translated_352_residues
MRILVTGGCGFIGSALVLHLVQDLGHEVLTLDAMTYAANPISLQPLADDPRHRLEQADICDPARVHALYAEFRPEAVMHL
AAESHVDRSITDPGAFVRTNVIGTQVMLDGARTHWESLDREAKATFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSA
SKAASDHLARAWHETYGLPVLVTNCSNNYGPRHFPEKLIPLMILAALEGKPLPVYGDGLNERDWIHVEDHARGLVAVLER
GRLGETYLLGGRSVRNNLAVVKALCAAFDQLKPENGPHERLISFVADRPGHDRRYAIDPSKAEAEVGWRPTKVFEEALTE
TVRWYLDNEAWWRPIREGRYSGERLGLAPKSA
>Mature_352_residues
MRILVTGGCGFIGSALVLHLVQDLGHEVLTLDAMTYAANPISLQPLADDPRHRLEQADICDPARVHALYAEFRPEAVMHL
AAESHVDRSITDPGAFVRTNVIGTQVMLDGARTHWESLDREAKATFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSA
SKAASDHLARAWHETYGLPVLVTNCSNNYGPRHFPEKLIPLMILAALEGKPLPVYGDGLNERDWIHVEDHARGLVAVLER
GRLGETYLLGGRSVRNNLAVVKALCAAFDQLKPENGPHERLISFVADRPGHDRRYAIDPSKAEAEVGWRPTKVFEEALTE
TVRWYLDNEAWWRPIREGRYSGERLGLAPKSA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=33.1360946745562, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=27.3273273273273, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI56237023, Length=362, Percent_Identity=26.2430939226519, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI56118217, Length=362, Percent_Identity=26.2430939226519, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI189083684, Length=362, Percent_Identity=26.2430939226519, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=56.8181818181818, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1788353, Length=349, Percent_Identity=54.1547277936963, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=28.3667621776504, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=26.5536723163842, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=328, Percent_Identity=33.5365853658537, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=332, Percent_Identity=30.421686746988, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=25.1515151515152, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=367, Percent_Identity=29.1553133514986, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=369, Percent_Identity=28.9972899728997, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319493, Length=376, Percent_Identity=23.936170212766, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=26.1127596439169, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=26.4623955431755, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24158427, Length=346, Percent_Identity=24.8554913294798, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39346; Mature: 39346

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGCGFIGSALVLHLVQDLGHEVLTLDAMTYAANPISLQPLADDPRHRLEQADIC
CEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCEECCCCCCHHHHHHHCCCC
DPARVHALYAEFRPEAVMHLAAESHVDRSITDPGAFVRTNVIGTQVMLDGARTHWESLDR
CHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHEEECCHHHHHHHHHH
EAKATFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSASKAASDHLARAWHETYGLPV
HHHHEEEEEEEEHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
LVTNCSNNYGPRHFPEKLIPLMILAALEGKPLPVYGDGLNERDWIHVEDHARGLVAVLER
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHC
GRLGETYLLGGRSVRNNLAVVKALCAAFDQLKPENGPHERLISFVADRPGHDRRYAIDPS
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCC
KAEAEVGWRPTKVFEEALTETVRWYLDNEAWWRPIREGRYSGERLGLAPKSA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRILVTGGCGFIGSALVLHLVQDLGHEVLTLDAMTYAANPISLQPLADDPRHRLEQADIC
CEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCEECCCCCCHHHHHHHCCCC
DPARVHALYAEFRPEAVMHLAAESHVDRSITDPGAFVRTNVIGTQVMLDGARTHWESLDR
CHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHEEECCHHHHHHHHHH
EAKATFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSASKAASDHLARAWHETYGLPV
HHHHEEEEEEEEHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
LVTNCSNNYGPRHFPEKLIPLMILAALEGKPLPVYGDGLNERDWIHVEDHARGLVAVLER
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHC
GRLGETYLLGGRSVRNNLAVVKALCAAFDQLKPENGPHERLISFVADRPGHDRRYAIDPS
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCC
KAEAEVGWRPTKVFEEALTETVRWYLDNEAWWRPIREGRYSGERLGLAPKSA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]