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The map label for this gene is nodQ [H]

Identifier: 218531753

GI number: 218531753

Start: 4040537

End: 4041169

Strand: Reverse

Name: nodQ [H]

Synonym: Mchl_3825

Alternate gene names: 218531753

Gene position: 4041169-4040537 (Counterclockwise)

Preceding gene: 218531755

Following gene: 218531750

Centisome position: 69.94

GC content: 60.35

Gene sequence:

>633_bases
ATGCCCGGGCAAAACATCATCTGGCAGGATCTGCAGCCACGCGAGCGTCGATGGGAGCGGATGGGGCAGCGCCCGGCCAT
TGCATGGCTCACCGGCCTTTCTGGAGCTGGAAAGTCGACGATCGCCAACGTCGTGGACCGGGCACTGACTCAGGCGGGCC
GTTGCAGCATGGTGCTCGACGGCGACAACCTGCGCCATGGGCTCAATCGCGACCTTGGCTTCACGTCTGCCGACCGAGTA
GAAAACATCCGGCGCACGGCTGAGGCAGCCCGGCTGATGGCGGACACCGGACTTGTGGTGATCGTTTCGCTCATCTCGCC
TTTCCGCGCCGAGCGCGCGGCGGCAAGGGCCATTGCGGGGGATATCCCTTTCCTTGAAATATTCATTGATGCGCCACTTG
CGGTCTGCGAAGCGCGTGATCCAAAGGGCCTCTACGGGCGCGCTCGCGCCGGTAAGATTCCGAGCTTTACCGGGGTCTCG
GCTCCTTATGAGGCCCCGCAGGCTGCAGATCTAGTCATAAATACGCATCATTTTCCGCCTGAGCAGTCAAGTCAAGACAT
AATTGCACGATTGCTTGAGTCCAGCGGCTGCTTTCCGGAAACCTCACCGGAAGTACTCAACTATAAGGCTTGA

Upstream 100 bases:

>100_bases
TTGAGAGCGGCAGCGTGAAAAAATCACCCTAGGTTAACGACAATCCGGCGGAAAACTCGTTCGACGTGTCACCCTAAACC
TTAGCGCTACGAGAAGGAAA

Downstream 100 bases:

>100_bases
GATAAACAAGGCGGTAGAGCGCCAAGCATCTGCGCAAGCCTCTTTATCGAAATCGCTATTCATCAAGCAGCCCTGCCCGG
TTCCCCGGCGCAGGGCTTTT

Product: adenylyl-sulfate kinase

Products: NA

Alternate protein names: Nodulation protein Q; Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPGQNIIWQDLQPRERRWERMGQRPAIAWLTGLSGAGKSTIANVVDRALTQAGRCSMVLDGDNLRHGLNRDLGFTSADRV
ENIRRTAEAARLMADTGLVVIVSLISPFRAERAAARAIAGDIPFLEIFIDAPLAVCEARDPKGLYGRARAGKIPSFTGVS
APYEAPQAADLVINTHHFPPEQSSQDIIARLLESSGCFPETSPEVLNYKA

Sequences:

>Translated_210_residues
MPGQNIIWQDLQPRERRWERMGQRPAIAWLTGLSGAGKSTIANVVDRALTQAGRCSMVLDGDNLRHGLNRDLGFTSADRV
ENIRRTAEAARLMADTGLVVIVSLISPFRAERAAARAIAGDIPFLEIFIDAPLAVCEARDPKGLYGRARAGKIPSFTGVS
APYEAPQAADLVINTHHFPPEQSSQDIIARLLESSGCFPETSPEVLNYKA
>Mature_209_residues
PGQNIIWQDLQPRERRWERMGQRPAIAWLTGLSGAGKSTIANVVDRALTQAGRCSMVLDGDNLRHGLNRDLGFTSADRVE
NIRRTAEAARLMADTGLVVIVSLISPFRAERAAARAIAGDIPFLEIFIDAPLAVCEARDPKGLYGRARAGKIPSFTGVSA
PYEAPQAADLVINTHHFPPEQSSQDIIARLLESSGCFPETSPEVLNYKA

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG0529

COG function: function code P; Adenylylsulfate kinase and related kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI34447231, Length=184, Percent_Identity=49.4565217391304, Blast_Score=176, Evalue=1e-44,
Organism=Homo sapiens, GI62912492, Length=184, Percent_Identity=49.4565217391304, Blast_Score=176, Evalue=1e-44,
Organism=Homo sapiens, GI46094058, Length=176, Percent_Identity=49.4318181818182, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1789107, Length=197, Percent_Identity=47.2081218274112, Blast_Score=194, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542422, Length=208, Percent_Identity=43.2692307692308, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322852, Length=191, Percent_Identity=47.6439790575916, Blast_Score=172, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24667032, Length=186, Percent_Identity=50.5376344086022, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24667028, Length=186, Percent_Identity=50.5376344086022, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24667036, Length=186, Percent_Identity=50.5376344086022, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI116007838, Length=186, Percent_Identity=50.5376344086022, Blast_Score=183, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24667044, Length=186, Percent_Identity=50.5376344086022, Blast_Score=183, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24667040, Length=186, Percent_Identity=50.5376344086022, Blast_Score=183, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 22862; Mature: 22731

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGQNIIWQDLQPRERRWERMGQRPAIAWLTGLSGAGKSTIANVVDRALTQAGRCSMVLD
CCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEC
GDNLRHGLNRDLGFTSADRVENIRRTAEAARLMADTGLVVIVSLISPFRAERAAARAIAG
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
DIPFLEIFIDAPLAVCEARDPKGLYGRARAGKIPSFTGVSAPYEAPQAADLVINTHHFPP
CCCEEEHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
EQSSQDIIARLLESSGCFPETSPEVLNYKA
CCCHHHHHHHHHHHCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
PGQNIIWQDLQPRERRWERMGQRPAIAWLTGLSGAGKSTIANVVDRALTQAGRCSMVLD
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEC
GDNLRHGLNRDLGFTSADRVENIRRTAEAARLMADTGLVVIVSLISPFRAERAAARAIAG
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
DIPFLEIFIDAPLAVCEARDPKGLYGRARAGKIPSFTGVSAPYEAPQAADLVINTHHFPP
CCCEEEHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
EQSSQDIIARLLESSGCFPETSPEVLNYKA
CCCHHHHHHHHHHHCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA