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The map label for this gene is fixL [H]

Identifier: 218531744

GI number: 218531744

Start: 4026192

End: 4027739

Strand: Reverse

Name: fixL [H]

Synonym: Mchl_3814

Alternate gene names: 218531744

Gene position: 4027739-4026192 (Counterclockwise)

Preceding gene: 218531747

Following gene: 218531743

Centisome position: 69.71

GC content: 69.19

Gene sequence:

>1548_bases
ATGGAATCGATGACGCTTTCCATGGCCGCGGCTGAGGCGATTCAGGGGGGCGGCGAAGCGCTGACGGCCGAGGACTTCCG
GCAGACCCTCCACGAGGTCGGTGTCTGCATCTGGTCGCTGGACATTCCCACCGGCCGCGTCAGCGCTTCGCAGACCTGCG
GCTGCCTCTTCGGTATTCCAACCGAACGTCTGACGAGCTTTGCCGCGACCCAGGATCTGGTCCACCCGGACGACCGCCAA
GCCCGCGCTCACGCCATCGAGAGCGTGCTGCGTGACGGCGGCAGTTACGAGATCGAATACCGTGTCGTGCTGCCGAATGG
GCGGGGTGGCTGGCTGCGCTCGCGAGGGCAGGTGCATCTCGACGCCGAAGGCCGGCCCCACCGGCACCGCGGGGTCGTCT
TCAGCATCGAAGAGCAGAAGCAGGTGGAGGCGGAGCTGCGCGCCCGCGAGGCCCATCTCCGCTCGATCCTCGACACGATG
CCGGAGGCGATGGTGGTCATCGACGAGGCGGGACTGATCCACTCGTTCAACCCGGCGGCCGAACGCCTCTTCGGCTACGC
GGCCGGCGAGGCGATCGGGCAGGACGTCCGCATCCTGATGCCGGAGGCGATGCAGGATGGACATGCCGCCGACCTCGAGC
GCTATCGGCAGACGCGCCAGCGCCACATCATCGGCACCACGCGGTGCGTGACGGGCCGACGGCATGACGGCTCGACCTTT
CCGATGGAGCTGGCCATCGGCGAGATGCATTCGGGCGAGCGGACCTTCTTCACCGGCTTCATCAACGACCTCACCGCGCA
GCGGCGCACCGAGGCGCGGCTTCAGGAACTCCAGTCCGAGCTGGGGCATGTCTCCCGCTTGAGCGCCATGGGCGAGATGG
CGACGACGCTGGCCCACGAGCTGAATCAGCCGCTCGGTGCCATCACCAACTACACCAACGGCTGCCGCCGCCTCCTCGCC
CATCCCGACCCCGAGACCATCGCCAGGGCACAGGAGGTTCTCGACAAGGCGGCCGAGCAGGCGCTGCGGGCCCGGCAGAT
CATCGCCCGCCTGCGGGAGTTCGTCGCCCGTGGCGAGACGGAGAAACGGGTCGAGCCGGTCGCGACGATGATCGAGGAGG
CCGGCGCCCTGACCCTGGCGGCGGCCGGCGAGCAGGGCATCACGGCCCACGTCGTGCCGGATCCGCGGGTCGGATCGGTC
TTGGTCGACCGGGTTCAGGTGCAGCAGGTTCTGGTCAACCTGATGCGCAATGCCTGCGAGGCGATGCAGCGCAGCAGCCG
GCGCGAGCTGACCGTCGCGACGCGACGGGTTTCGCCGGATCTGGCCGAGGTCGCGGTGTCGGATACCGGCCCCGGTATCG
CCGAGGAGGTCGCCGACCGGCTGTTCCAGCCCTTCGTCACCACCAAGGAGGCCGGGATGGGCGTCGGCCTCTCGATTTCC
CGCACCATCATCGAGGCGCATGGCGGCCGCCTCTGGGTCGAGCCCAACGCCGCGGGCGGGGCGACCTTCCGGCTGACCCT
GCCGACCGCACCCGAGAGAGGTCGGTAA

Upstream 100 bases:

>100_bases
CGTTACGAGTTGAATGATTGGCTTTTTATTTATCAAATGACATGATTTATGTAGAATTTATTCAAAAATTATCCCCTCAT
CCTACGCAGAGACTTCCGGG

Downstream 100 bases:

>100_bases
CCCATGGCCCCTGCCCTCGTGCATGTCATCGACGACGACCCCGCCATGCGCGACTCGGTCGCGTTCCTTCTCGACACGGC
CGGCTTCGACGTGCGGCTCT

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MESMTLSMAAAEAIQGGGEALTAEDFRQTLHEVGVCIWSLDIPTGRVSASQTCGCLFGIPTERLTSFAATQDLVHPDDRQ
ARAHAIESVLRDGGSYEIEYRVVLPNGRGGWLRSRGQVHLDAEGRPHRHRGVVFSIEEQKQVEAELRAREAHLRSILDTM
PEAMVVIDEAGLIHSFNPAAERLFGYAAGEAIGQDVRILMPEAMQDGHAADLERYRQTRQRHIIGTTRCVTGRRHDGSTF
PMELAIGEMHSGERTFFTGFINDLTAQRRTEARLQELQSELGHVSRLSAMGEMATTLAHELNQPLGAITNYTNGCRRLLA
HPDPETIARAQEVLDKAAEQALRARQIIARLREFVARGETEKRVEPVATMIEEAGALTLAAAGEQGITAHVVPDPRVGSV
LVDRVQVQQVLVNLMRNACEAMQRSSRRELTVATRRVSPDLAEVAVSDTGPGIAEEVADRLFQPFVTTKEAGMGVGLSIS
RTIIEAHGGRLWVEPNAAGGATFRLTLPTAPERGR

Sequences:

>Translated_515_residues
MESMTLSMAAAEAIQGGGEALTAEDFRQTLHEVGVCIWSLDIPTGRVSASQTCGCLFGIPTERLTSFAATQDLVHPDDRQ
ARAHAIESVLRDGGSYEIEYRVVLPNGRGGWLRSRGQVHLDAEGRPHRHRGVVFSIEEQKQVEAELRAREAHLRSILDTM
PEAMVVIDEAGLIHSFNPAAERLFGYAAGEAIGQDVRILMPEAMQDGHAADLERYRQTRQRHIIGTTRCVTGRRHDGSTF
PMELAIGEMHSGERTFFTGFINDLTAQRRTEARLQELQSELGHVSRLSAMGEMATTLAHELNQPLGAITNYTNGCRRLLA
HPDPETIARAQEVLDKAAEQALRARQIIARLREFVARGETEKRVEPVATMIEEAGALTLAAAGEQGITAHVVPDPRVGSV
LVDRVQVQQVLVNLMRNACEAMQRSSRRELTVATRRVSPDLAEVAVSDTGPGIAEEVADRLFQPFVTTKEAGMGVGLSIS
RTIIEAHGGRLWVEPNAAGGATFRLTLPTAPERGR
>Mature_515_residues
MESMTLSMAAAEAIQGGGEALTAEDFRQTLHEVGVCIWSLDIPTGRVSASQTCGCLFGIPTERLTSFAATQDLVHPDDRQ
ARAHAIESVLRDGGSYEIEYRVVLPNGRGGWLRSRGQVHLDAEGRPHRHRGVVFSIEEQKQVEAELRAREAHLRSILDTM
PEAMVVIDEAGLIHSFNPAAERLFGYAAGEAIGQDVRILMPEAMQDGHAADLERYRQTRQRHIIGTTRCVTGRRHDGSTF
PMELAIGEMHSGERTFFTGFINDLTAQRRTEARLQELQSELGHVSRLSAMGEMATTLAHELNQPLGAITNYTNGCRRLLA
HPDPETIARAQEVLDKAAEQALRARQIIARLREFVARGETEKRVEPVATMIEEAGALTLAAAGEQGITAHVVPDPRVGSV
LVDRVQVQQVLVNLMRNACEAMQRSSRRELTVATRRVSPDLAEVAVSDTGPGIAEEVADRLFQPFVTTKEAGMGVGLSIS
RTIIEAHGGRLWVEPNAAGGATFRLTLPTAPERGR

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=361, Percent_Identity=27.1468144044321, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790436, Length=246, Percent_Identity=28.4552845528455, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790300, Length=374, Percent_Identity=26.7379679144385, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI48994928, Length=387, Percent_Identity=24.8062015503876, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=28.125, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=25.8687258687259, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56340; Mature: 56340

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESMTLSMAAAEAIQGGGEALTAEDFRQTLHEVGVCIWSLDIPTGRVSASQTCGCLFGIP
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEECCC
TERLTSFAATQDLVHPDDRQARAHAIESVLRDGGSYEIEYRVVLPNGRGGWLRSRGQVHL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCEEE
DAEGRPHRHRGVVFSIEEQKQVEAELRAREAHLRSILDTMPEAMVVIDEAGLIHSFNPAA
CCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
ERLFGYAAGEAIGQDVRILMPEAMQDGHAADLERYRQTRQRHIIGTTRCVTGRRHDGSTF
HHHHHHHHHHHHCCCCEEECHHHHCCCCHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCC
PMELAIGEMHSGERTFFTGFINDLTAQRRTEARLQELQSELGHVSRLSAMGEMATTLAHE
CCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNQPLGAITNYTNGCRRLLAHPDPETIARAQEVLDKAAEQALRARQIIARLREFVARGET
HCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EKRVEPVATMIEEAGALTLAAAGEQGITAHVVPDPRVGSVLVDRVQVQQVLVNLMRNACE
HHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AMQRSSRRELTVATRRVSPDLAEVAVSDTGPGIAEEVADRLFQPFVTTKEAGMGVGLSIS
HHHHCCCCCEEHHHHHCCCCHHHHEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCHHH
RTIIEAHGGRLWVEPNAAGGATFRLTLPTAPERGR
HHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MESMTLSMAAAEAIQGGGEALTAEDFRQTLHEVGVCIWSLDIPTGRVSASQTCGCLFGIP
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEECCC
TERLTSFAATQDLVHPDDRQARAHAIESVLRDGGSYEIEYRVVLPNGRGGWLRSRGQVHL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCEEE
DAEGRPHRHRGVVFSIEEQKQVEAELRAREAHLRSILDTMPEAMVVIDEAGLIHSFNPAA
CCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
ERLFGYAAGEAIGQDVRILMPEAMQDGHAADLERYRQTRQRHIIGTTRCVTGRRHDGSTF
HHHHHHHHHHHHCCCCEEECHHHHCCCCHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCC
PMELAIGEMHSGERTFFTGFINDLTAQRRTEARLQELQSELGHVSRLSAMGEMATTLAHE
CCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNQPLGAITNYTNGCRRLLAHPDPETIARAQEVLDKAAEQALRARQIIARLREFVARGET
HCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EKRVEPVATMIEEAGALTLAAAGEQGITAHVVPDPRVGSVLVDRVQVQQVLVNLMRNACE
HHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AMQRSSRRELTVATRRVSPDLAEVAVSDTGPGIAEEVADRLFQPFVTTKEAGMGVGLSIS
HHHHCCCCCEEHHHHHCCCCHHHHEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCHHH
RTIIEAHGGRLWVEPNAAGGATFRLTLPTAPERGR
HHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]