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The map label for this gene is rlmE [H]

Identifier: 218531672

GI number: 218531672

Start: 3952036

End: 3952755

Strand: Reverse

Name: rlmE [H]

Synonym: Mchl_3741

Alternate gene names: 218531672

Gene position: 3952755-3952036 (Counterclockwise)

Preceding gene: 218531673

Following gene: 218531671

Centisome position: 68.41

GC content: 70.28

Gene sequence:

>720_bases
ATGAGCGACCGGCGAGGCGGGACGGGGGGCCTGCGCGGCGACCTGAAGCAGCGGGTGAAGACCGGGCGCGGGCGCACCCT
CTCCCAGAAGCGCTGGCTGGAACGCCAGCTCAACGACCCGTACGTCGCCCGCGCCAAGCGCGAGGGCTACCGTTCCCGCG
CGGCGTTCAAGCTTCTCGAAATCGACGAGCGCTTCAAGCTGTTGAAGCCCGGACAGCGCATCGTCGATCTCGGCGCCGCC
CCCGGTGGCTGGTCGCAGGTGGCGGCGCGGATCCTCGGCGAGAGCGGCCGCATCGTCGGCATCGATCTGCTTGAGATCGA
GCCGATGCCGGGCGCGACCTTCATCACCCTCGATTTCCTCGATCCCTCCGCACCCGAGCGCTTGACCGAGCTGCTGGGCG
GCCGGGCCGACCTCGTCCTGTCCGACATGGCCGCCAACACCACCGGCCACAAGAAGACCGACCACCTGCGCATCATCGGG
CTCGCCGAGACCGCCGCGGTCTTCGCCCGCGAGATCCTGGCGCCCGGCGGCGCCTATCTCGCCAAGGTGTTCCAGGGCGG
CACCGAGGGCGATCTGCTGACCGAGCTGAAGCGCGACTTCGCCACCGTGCGCCACGTCAAGCCGCAGGCGAGCCGGGCGG
ATTCGAGCGAACTCTACGTGCTGGCCACGGGCTTTCGCGGCCAGGCCGGCGCGAAGGACGCGGCGGACGAACAGGACTAA

Upstream 100 bases:

>100_bases
CTCCCCGGACGGAACGTCTTCACGGACCCGCCGCGCCGCGATAGGGACCATCCTGCGGCTGTGAGCGCATCGGTGCGTTC
CGATGCTTGAGGACAGGATC

Downstream 100 bases:

>100_bases
CCGTCACCGGCGGCTGCGCCCGGCCTTCCTTTCGTGGCACGATGCCTGCGCGCCGTGCCCGAGAGCCACGTAAGGAAGGC
CCCGATGACTGCCCAACCCC

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSDRRGGTGGLRGDLKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAFKLLEIDERFKLLKPGQRIVDLGAA
PGGWSQVAARILGESGRIVGIDLLEIEPMPGATFITLDFLDPSAPERLTELLGGRADLVLSDMAANTTGHKKTDHLRIIG
LAETAAVFAREILAPGGAYLAKVFQGGTEGDLLTELKRDFATVRHVKPQASRADSSELYVLATGFRGQAGAKDAADEQD

Sequences:

>Translated_239_residues
MSDRRGGTGGLRGDLKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAFKLLEIDERFKLLKPGQRIVDLGAA
PGGWSQVAARILGESGRIVGIDLLEIEPMPGATFITLDFLDPSAPERLTELLGGRADLVLSDMAANTTGHKKTDHLRIIG
LAETAAVFAREILAPGGAYLAKVFQGGTEGDLLTELKRDFATVRHVKPQASRADSSELYVLATGFRGQAGAKDAADEQD
>Mature_238_residues
SDRRGGTGGLRGDLKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAFKLLEIDERFKLLKPGQRIVDLGAAP
GGWSQVAARILGESGRIVGIDLLEIEPMPGATFITLDFLDPSAPERLTELLGGRADLVLSDMAANTTGHKKTDHLRIIGL
AETAAVFAREILAPGGAYLAKVFQGGTEGDLLTELKRDFATVRHVKPQASRADSSELYVLATGFRGQAGAKDAADEQD

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=218, Percent_Identity=45.8715596330275, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=38.2978723404255, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI29029591, Length=205, Percent_Identity=38.0487804878049, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI29029589, Length=205, Percent_Identity=38.0487804878049, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI7110661, Length=205, Percent_Identity=38.0487804878049, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1789569, Length=205, Percent_Identity=44.8780487804878, Blast_Score=176, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17553474, Length=202, Percent_Identity=40.0990099009901, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17554650, Length=200, Percent_Identity=35, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=36.2694300518135, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=36.2694300518135, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71987561, Length=196, Percent_Identity=36.2244897959184, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=36.1809045226131, Blast_Score=118, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6321302, Length=101, Percent_Identity=47.5247524752475, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=31.413612565445, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356387, Length=222, Percent_Identity=40.5405405405405, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=37.2340425531915, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648639, Length=218, Percent_Identity=37.1559633027523, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24647580, Length=213, Percent_Identity=34.2723004694836, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 25967; Mature: 25836

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRRGGTGGLRGDLKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAFKLLE
CCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IDERFKLLKPGQRIVDLGAAPGGWSQVAARILGESGRIVGIDLLEIEPMPGATFITLDFL
HHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEECC
DPSAPERLTELLGGRADLVLSDMAANTTGHKKTDHLRIIGLAETAAVFAREILAPGGAYL
CCCCHHHHHHHHCCCHHEEEHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
AKVFQGGTEGDLLTELKRDFATVRHVKPQASRADSSELYVLATGFRGQAGAKDAADEQD
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDRRGGTGGLRGDLKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAFKLLE
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IDERFKLLKPGQRIVDLGAAPGGWSQVAARILGESGRIVGIDLLEIEPMPGATFITLDFL
HHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEECC
DPSAPERLTELLGGRADLVLSDMAANTTGHKKTDHLRIIGLAETAAVFAREILAPGGAYL
CCCCHHHHHHHHCCCHHEEEHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
AKVFQGGTEGDLLTELKRDFATVRHVKPQASRADSSELYVLATGFRGQAGAKDAADEQD
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA