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The map label for this gene is fmdA [H]

Identifier: 218531615

GI number: 218531615

Start: 3882049

End: 3883278

Strand: Reverse

Name: fmdA [H]

Synonym: Mchl_3684

Alternate gene names: 218531615

Gene position: 3883278-3882049 (Counterclockwise)

Preceding gene: 218531616

Following gene: 218531614

Centisome position: 67.21

GC content: 63.9

Gene sequence:

>1230_bases
ATGCCCGAGACCCTGATTAAGGTCGATCTCACGCAGTCGGCCTACGACAACGACATGGTGCACAACCGCTGGCACCCGGA
CATTCCGATGGTCGCGACCGTGAAGCCCGGCGACGACTTCATCGTCGAGACCTATGACTGGACCGGCGGCTTCATCAAGA
ACAACGATTCCGCCGACGACGTGCGCGACATCGACCTGTCGATCGTGCACTTCCTGTCCGGCCCGATCGGCGTCGAGGGT
GCCGAGCCCGGCGACCTGCTCGTGGTCGATCTGCTCGATATCGGCGCCAAGCCCGAGAGCCAGTGGGGCTTCAACGGCTT
CTTCTCGAAGAACAACGGCGGCGGCTTTCTCGACGAACACTTCCCCCAGGCCCAGAAGTCGATCTGGGATTTCGAGGGCA
TGTTTACCAAGTCGCGCCACGTGCCCGGCGTGCGCTTCCCCGGCCTGATCCATCCCGGCCTGATCGGCTGTCTGCCCGAT
CCGAAGATGCTGGAGACCTGGAACACCCGCGAGAAGGCGCTCTACGACACCAACCCGAGCCGAGTGCCGGCGCTGGCCAC
CCTCCCCTTCGGCCCGACCGCGCATATGGGCCGGCTGAAGGGTGACGCGAAGGACAACGCCGCGGCGACGGGCGCCCGTA
CGGTGCCGGGACGTGAGCATGGCGGCAATTGCGACATTAAGGATCTGTCGCGCGGCTCGAAGATCTACTTCCCCGTCTAC
GTGCCCGGCGCCGGCCTTTCCATGGGCGACCTGCATTTCAGCCAGGGCGACGGCGAGATCACCTTCTGCGGCGCTATCGA
GATGGCAGGCTGGGTCCACCTCAAGGTCAGCCTGATCAAGGACGGTATGGCCAAGTACGGGATCAAGAACCCGATCTTCA
AGCCGTCGCCGATCACCCCGAAATACGACGATCACCTGATCTTCGAGGGTGTCTCGGTCGACGAGTACGGCAAGCAGCAT
TACCTCGACGTCACGGTCGCCTACCGCCAAGCCTGCCTCAACGCGATCGAGTACCTGAAGAAGTTCGGCTACTCGGGCGC
CCAGGCCTACTCGATCCTCGGCACCGCCCCGGTCCAGGGCCACATCTCCGGCGTCGTCGATATCCCGAACGCCTGCGCCA
CGCTCTGGATTCCGACCGGCATCTTCGACTTCGACATCAACCCGTCCGAGGCCGGGCCGACGAAGTTCCTCGACGGCTCG
ATCCAGATGCCGCTCTCGCCGGATCTCTGA

Upstream 100 bases:

>100_bases
ACGAAGAACGACAACAACGATCCACGGGGGCGGCCGGACCAGCACCGGACACGCAACGGGGACGGCTGCCAAAAATCCGG
ACTGTAAAGGAGTAAAGGAA

Downstream 100 bases:

>100_bases
ATCTCTTGGTTTGCCGCCCTCTCCGGGCGGCAAGCCGCACACACTTTGCCTGAGAGTGCTTGAGATGCCGGTCTACGATT
ACGCCTGCGAGAGCTGTGGT

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MPETLIKVDLTQSAYDNDMVHNRWHPDIPMVATVKPGDDFIVETYDWTGGFIKNNDSADDVRDIDLSIVHFLSGPIGVEG
AEPGDLLVVDLLDIGAKPESQWGFNGFFSKNNGGGFLDEHFPQAQKSIWDFEGMFTKSRHVPGVRFPGLIHPGLIGCLPD
PKMLETWNTREKALYDTNPSRVPALATLPFGPTAHMGRLKGDAKDNAAATGARTVPGREHGGNCDIKDLSRGSKIYFPVY
VPGAGLSMGDLHFSQGDGEITFCGAIEMAGWVHLKVSLIKDGMAKYGIKNPIFKPSPITPKYDDHLIFEGVSVDEYGKQH
YLDVTVAYRQACLNAIEYLKKFGYSGAQAYSILGTAPVQGHISGVVDIPNACATLWIPTGIFDFDINPSEAGPTKFLDGS
IQMPLSPDL

Sequences:

>Translated_409_residues
MPETLIKVDLTQSAYDNDMVHNRWHPDIPMVATVKPGDDFIVETYDWTGGFIKNNDSADDVRDIDLSIVHFLSGPIGVEG
AEPGDLLVVDLLDIGAKPESQWGFNGFFSKNNGGGFLDEHFPQAQKSIWDFEGMFTKSRHVPGVRFPGLIHPGLIGCLPD
PKMLETWNTREKALYDTNPSRVPALATLPFGPTAHMGRLKGDAKDNAAATGARTVPGREHGGNCDIKDLSRGSKIYFPVY
VPGAGLSMGDLHFSQGDGEITFCGAIEMAGWVHLKVSLIKDGMAKYGIKNPIFKPSPITPKYDDHLIFEGVSVDEYGKQH
YLDVTVAYRQACLNAIEYLKKFGYSGAQAYSILGTAPVQGHISGVVDIPNACATLWIPTGIFDFDINPSEAGPTKFLDGS
IQMPLSPDL
>Mature_408_residues
PETLIKVDLTQSAYDNDMVHNRWHPDIPMVATVKPGDDFIVETYDWTGGFIKNNDSADDVRDIDLSIVHFLSGPIGVEGA
EPGDLLVVDLLDIGAKPESQWGFNGFFSKNNGGGFLDEHFPQAQKSIWDFEGMFTKSRHVPGVRFPGLIHPGLIGCLPDP
KMLETWNTREKALYDTNPSRVPALATLPFGPTAHMGRLKGDAKDNAAATGARTVPGREHGGNCDIKDLSRGSKIYFPVYV
PGAGLSMGDLHFSQGDGEITFCGAIEMAGWVHLKVSLIKDGMAKYGIKNPIFKPSPITPKYDDHLIFEGVSVDEYGKQHY
LDVTVAYRQACLNAIEYLKKFGYSGAQAYSILGTAPVQGHISGVVDIPNACATLWIPTGIFDFDINPSEAGPTKFLDGSI
QMPLSPDL

Specific function: Hydrolyzes formamide with the production of ammonia which can be used as a source of nitrogen for growth. Also acts, more slowly, on acetamide, propanamide and butanamide [H]

COG id: COG2421

COG function: function code C; Predicted acetamidase/formamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetamidase/formamidase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004304 [H]

Pfam domain/function: PF03069 FmdA_AmdA [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 44558; Mature: 44427

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETLIKVDLTQSAYDNDMVHNRWHPDIPMVATVKPGDDFIVETYDWTGGFIKNNDSADD
CCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEECCCCCCC
VRDIDLSIVHFLSGPIGVEGAEPGDLLVVDLLDIGAKPESQWGFNGFFSKNNGGGFLDEH
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHH
FPQAQKSIWDFEGMFTKSRHVPGVRFPGLIHPGLIGCLPDPKMLETWNTREKALYDTNPS
CCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEECCCCCHHHHCCCCCCCEEECCCCC
RVPALATLPFGPTAHMGRLKGDAKDNAAATGARTVPGREHGGNCDIKDLSRGSKIYFPVY
CCCEEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEE
VPGAGLSMGDLHFSQGDGEITFCGAIEMAGWVHLKVSLIKDGMAKYGIKNPIFKPSPITP
ECCCCCEECCEEEECCCCCEEEEEEECCCCEEEEEEEEHHCCHHHCCCCCCCCCCCCCCC
KYDDHLIFEGVSVDEYGKQHYLDVTVAYRQACLNAIEYLKKFGYSGAQAYSILGTAPVQG
CCCCEEEEECCCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
HISGVVDIPNACATLWIPTGIFDFDINPSEAGPTKFLDGSIQMPLSPDL
CCEEEEECCCCCEEEEEECCEEEECCCCCCCCCCEEECCCEECCCCCCC
>Mature Secondary Structure 
PETLIKVDLTQSAYDNDMVHNRWHPDIPMVATVKPGDDFIVETYDWTGGFIKNNDSADD
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEECCCCCCC
VRDIDLSIVHFLSGPIGVEGAEPGDLLVVDLLDIGAKPESQWGFNGFFSKNNGGGFLDEH
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHH
FPQAQKSIWDFEGMFTKSRHVPGVRFPGLIHPGLIGCLPDPKMLETWNTREKALYDTNPS
CCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEECCCCCHHHHCCCCCCCEEECCCCC
RVPALATLPFGPTAHMGRLKGDAKDNAAATGARTVPGREHGGNCDIKDLSRGSKIYFPVY
CCCEEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEE
VPGAGLSMGDLHFSQGDGEITFCGAIEMAGWVHLKVSLIKDGMAKYGIKNPIFKPSPITP
ECCCCCEECCEEEECCCCCEEEEEEECCCCEEEEEEEEHHCCHHHCCCCCCCCCCCCCCC
KYDDHLIFEGVSVDEYGKQHYLDVTVAYRQACLNAIEYLKKFGYSGAQAYSILGTAPVQG
CCCCEEEEECCCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
HISGVVDIPNACATLWIPTGIFDFDINPSEAGPTKFLDGSIQMPLSPDL
CCEEEEECCCCCEEEEEECCEEEECCCCCCCCCCEEECCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8841393 [H]