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The map label for this gene is yieH [H]

Identifier: 218531548

GI number: 218531548

Start: 3807772

End: 3808458

Strand: Reverse

Name: yieH [H]

Synonym: Mchl_3616

Alternate gene names: 218531548

Gene position: 3808458-3807772 (Counterclockwise)

Preceding gene: 218531550

Following gene: 218531547

Centisome position: 65.91

GC content: 71.47

Gene sequence:

>687_bases
ATGACGGACGCCTTCCCCGCCTCGCCTTCCCTCGCCCTCGTGATCTTCGACTGCGACGGCGTGCTGATCGACAGCGAGCC
GATCAGCCTCGCGACCCTGACGCGCGGGCTCAACGGGATCGGGCTCGCGATCAGCGTCGACAGCGTGCGGGAGCGGTTTG
CCGGCACCTCCATGACCTCGATCATGGAGCGCGTCGCCCGCGAGGATGGGGTCACGGCTCCGGACGGCTTCGTCGAGCGC
GTGAAGGCGGAGACGCTCGCCGCCTTCGAGGCGGAGCTGGCCGCCATGGCCGGGATCGCCGAGGCACTGGGGCTCCTGAA
CCTGCCGTTCTGCGTCGCCTCCAGCAGCGACCCCGTGCGGCTGCGCCGCTCGCTCTCGCTCACCGGACTGCTGCCGCTGT
TCGAGGGGCGCGTCTTCTCCTCCGCGCAGGTGGCGCGCGGAAAGCCGTTCCCGGACCTGTTCCTGTTCGCGGCTGAACAG
ATGGGCATCGCGCCGGAACAGTGCCTCGTGATCGAGGACAGCGTGCCGGGGGTGCAGGCCGCGCGGGCGGCCGGAATGCG
GGTCGTCGGCTTCACCGGCGGCGGGCATTGGGGCCACGACCGGGCCGGACGGGATCTCCTCGATGCCGGGGCCGCCGCTA
TCTTCTCGGCCCATGCCGATCTCGGGCGCATCGTCGCCGGGGCATGA

Upstream 100 bases:

>100_bases
CGCGGGGCGCAGTTGGCGCCGGGAATGTGGTGCAACCCCGCTTCGAGCGATGGCCCGGCTGCGTTGCAGTGCTTCGATTG
CCGTGCGAGACGAGCCGCCG

Downstream 100 bases:

>100_bases
GGCCGGTTTCGGCCCGGGCTTTGCTAGATTTGGCGTGAGCCCTTCCCCGACAGACGGGACGCGCCGCGGATCGCTCGAAT
CGCGGCGCGAACTTCCTCTG

Product: HAD-superfamily hydrolase, subfamily IA, variant 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTDAFPASPSLALVIFDCDGVLIDSEPISLATLTRGLNGIGLAISVDSVRERFAGTSMTSIMERVAREDGVTAPDGFVER
VKAETLAAFEAELAAMAGIAEALGLLNLPFCVASSSDPVRLRRSLSLTGLLPLFEGRVFSSAQVARGKPFPDLFLFAAEQ
MGIAPEQCLVIEDSVPGVQAARAAGMRVVGFTGGGHWGHDRAGRDLLDAGAAAIFSAHADLGRIVAGA

Sequences:

>Translated_228_residues
MTDAFPASPSLALVIFDCDGVLIDSEPISLATLTRGLNGIGLAISVDSVRERFAGTSMTSIMERVAREDGVTAPDGFVER
VKAETLAAFEAELAAMAGIAEALGLLNLPFCVASSSDPVRLRRSLSLTGLLPLFEGRVFSSAQVARGKPFPDLFLFAAEQ
MGIAPEQCLVIEDSVPGVQAARAAGMRVVGFTGGGHWGHDRAGRDLLDAGAAAIFSAHADLGRIVAGA
>Mature_227_residues
TDAFPASPSLALVIFDCDGVLIDSEPISLATLTRGLNGIGLAISVDSVRERFAGTSMTSIMERVAREDGVTAPDGFVERV
KAETLAAFEAELAAMAGIAEALGLLNLPFCVASSSDPVRLRRSLSLTGLLPLFEGRVFSSAQVARGKPFPDLFLFAAEQM
GIAPEQCLVIEDSVPGVQAARAAGMRVVGFTGGGHWGHDRAGRDLLDAGAAAIFSAHADLGRIVAGA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=184, Percent_Identity=33.695652173913, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789046, Length=184, Percent_Identity=28.804347826087, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788021, Length=180, Percent_Identity=29.4444444444444, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789787, Length=210, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=4e-11,
Organism=Drosophila melanogaster, GI116008157, Length=185, Percent_Identity=34.5945945945946, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17137324, Length=186, Percent_Identity=32.7956989247312, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23656; Mature: 23525

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAFPASPSLALVIFDCDGVLIDSEPISLATLTRGLNGIGLAISVDSVRERFAGTSMTS
CCCCCCCCCCEEEEEEECCCEEECCCCCHHHHHHCCCCCEEEEEEHHHHHHHHCCCCHHH
IMERVAREDGVTAPDGFVERVKAETLAAFEAELAAMAGIAEALGLLNLPFCVASSSDPVR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHH
LRRSLSLTGLLPLFEGRVFSSAQVARGKPFPDLFLFAAEQMGIAPEQCLVIEDSVPGVQA
HHHHCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
ARAAGMRVVGFTGGGHWGHDRAGRDLLDAGAAAIFSAHADLGRIVAGA
HHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDAFPASPSLALVIFDCDGVLIDSEPISLATLTRGLNGIGLAISVDSVRERFAGTSMTS
CCCCCCCCCEEEEEEECCCEEECCCCCHHHHHHCCCCCEEEEEEHHHHHHHHCCCCHHH
IMERVAREDGVTAPDGFVERVKAETLAAFEAELAAMAGIAEALGLLNLPFCVASSSDPVR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHH
LRRSLSLTGLLPLFEGRVFSSAQVARGKPFPDLFLFAAEQMGIAPEQCLVIEDSVPGVQA
HHHHCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
ARAAGMRVVGFTGGGHWGHDRAGRDLLDAGAAAIFSAHADLGRIVAGA
HHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]