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The map label for this gene is fadA [H]

Identifier: 218531500

GI number: 218531500

Start: 3754782

End: 3755990

Strand: Reverse

Name: fadA [H]

Synonym: Mchl_3568

Alternate gene names: 218531500

Gene position: 3755990-3754782 (Counterclockwise)

Preceding gene: 218531501

Following gene: 218531499

Centisome position: 65.01

GC content: 69.98

Gene sequence:

>1209_bases
ATGCCCGAGGCCTACATCTACGATCACGTCCGCACCCCCCGCGGCCGCGGCAAGCCGGATGGTTCGCTGCACGAGGTGAC
GGCGTTGCGCCTCGCCGAGACGGCGTTGCGCGCGCTCAAGGACCGCAACGGCCTCGACACGCGGCAGGTGGACGACGTGG
TGCTCGGCTGCGTCGATCCGGTCGGCGAGGCCGGCGGCGACATCGCCCGCGCCGCCGCGCTCGTGGCTGATTACGGCACC
CACGTGCCCGGCGTGCAGATCAACCGCTTCTGCGCCTCGGGCCTCGACGCCATCAACTTCGCCGCGGCGCAGGTCATGGC
CGGCCAGCACGATCTTACCGTCGGCGGCGGTGTCGAATCGATGAGCCGCGTCGGCCTCGGCGCCTCGGGCGGCGCCTGGC
CGGTCGATCCGGCGATCGCGGTCAAATCCTACTTCATGCCGCAGGGCGTCTCGGCCGACCTCATCGCCACCAAGTACGGG
TTCAGCCGCGACGATTGCGACGCCTACGCCGTCGAGTCGCAGAAGCGCTCGGCCCAATCCTGGGGCGAGGGCCTGTTCAA
GAACTCCGTCGTGCCGGTGCGGGACATCAACGGCATCACCCTGCTCGACCGCGACGAGCACATGCGGCCGACGACCGACA
TGCAGTCGCTGGCGAGCCTGAAACCGTCCTTCGTGCAGATGGGCCAGATGGGCGGCTTCGACGCGGTAGCCGTCGATGCC
CACCCGGATGTCGAGGCGGTCCGTCACGTCCACCATGCCGGCAACTCGTCGGGCATCGTGGATGGCGCGGCGGCGGTGCT
GATCGGCTCGCGCGAGGCCGGCGACGCCGCGGGCCTGCGGCCCCGCGCCCGCATCCGGGCCTTCGCCAATATCGGCTCCG
ATCCGGCGCTGATGCTGACCGGCCCGGTCGATGTCACGAAGAAGGTGCTGGCGCGGGCCGGCATGAGCCTGTCCGACATC
GACCTGTTCGAGATCAACGAGGCCTTCGCCTCGGTGGTCCTGCGCTTCCATCAGGCCTTCGACCTCGACCCGGCGAAGGT
GAACGTCAACGGCGGCGCCATCGCCATGGGTCATCCGCTGGGTGCCACGGGCGCGATGATCCTCGGCACCGTGCTCGACG
AGTTGGAGCGCACCGGCAAGGAGCGGGCGCTCGTGACCCTGTGCATCGGCGCTGGCATGGGCACCGCCACGATCATCGAG
CGGGTGTGA

Upstream 100 bases:

>100_bases
CGACCGGAGCCGGCCCCTCACCCGCCCGCCCACGCGGGCACCCTCTCCCGCGAGGGAATGGGGGAGCCGCGGCAGCGATG
AAGGACACGAGGAAGCGCAT

Downstream 100 bases:

>100_bases
TCGGCCGGGCGGCGTCACGCCGCCGCCCTGCCCTGCCATCGCCGCGCAAACGCTGCTAAGGGCCTCCCACGCCGCGCCCT
TTTTCGATCGGGTGCGGCCC

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPEAYIYDHVRTPRGRGKPDGSLHEVTALRLAETALRALKDRNGLDTRQVDDVVLGCVDPVGEAGGDIARAAALVADYGT
HVPGVQINRFCASGLDAINFAAAQVMAGQHDLTVGGGVESMSRVGLGASGGAWPVDPAIAVKSYFMPQGVSADLIATKYG
FSRDDCDAYAVESQKRSAQSWGEGLFKNSVVPVRDINGITLLDRDEHMRPTTDMQSLASLKPSFVQMGQMGGFDAVAVDA
HPDVEAVRHVHHAGNSSGIVDGAAAVLIGSREAGDAAGLRPRARIRAFANIGSDPALMLTGPVDVTKKVLARAGMSLSDI
DLFEINEAFASVVLRFHQAFDLDPAKVNVNGGAIAMGHPLGATGAMILGTVLDELERTGKERALVTLCIGAGMGTATIIE
RV

Sequences:

>Translated_402_residues
MPEAYIYDHVRTPRGRGKPDGSLHEVTALRLAETALRALKDRNGLDTRQVDDVVLGCVDPVGEAGGDIARAAALVADYGT
HVPGVQINRFCASGLDAINFAAAQVMAGQHDLTVGGGVESMSRVGLGASGGAWPVDPAIAVKSYFMPQGVSADLIATKYG
FSRDDCDAYAVESQKRSAQSWGEGLFKNSVVPVRDINGITLLDRDEHMRPTTDMQSLASLKPSFVQMGQMGGFDAVAVDA
HPDVEAVRHVHHAGNSSGIVDGAAAVLIGSREAGDAAGLRPRARIRAFANIGSDPALMLTGPVDVTKKVLARAGMSLSDI
DLFEINEAFASVVLRFHQAFDLDPAKVNVNGGAIAMGHPLGATGAMILGTVLDELERTGKERALVTLCIGAGMGTATIIE
RV
>Mature_401_residues
PEAYIYDHVRTPRGRGKPDGSLHEVTALRLAETALRALKDRNGLDTRQVDDVVLGCVDPVGEAGGDIARAAALVADYGTH
VPGVQINRFCASGLDAINFAAAQVMAGQHDLTVGGGVESMSRVGLGASGGAWPVDPAIAVKSYFMPQGVSADLIATKYGF
SRDDCDAYAVESQKRSAQSWGEGLFKNSVVPVRDINGITLLDRDEHMRPTTDMQSLASLKPSFVQMGQMGGFDAVAVDAH
PDVEAVRHVHHAGNSSGIVDGAAAVLIGSREAGDAAGLRPRARIRAFANIGSDPALMLTGPVDVTKKVLARAGMSLSDID
LFEINEAFASVVLRFHQAFDLDPAKVNVNGGAIAMGHPLGATGAMILGTVLDELERTGKERALVTLCIGAGMGTATIIER
V

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=371, Percent_Identity=36.6576819407008, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=34.1708542713568, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI167614485, Length=407, Percent_Identity=33.9066339066339, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI4557237, Length=417, Percent_Identity=30.9352517985612, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=30.1123595505618, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI194353979, Length=124, Percent_Identity=43.5483870967742, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=41.3145539906103, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI48994986, Length=409, Percent_Identity=38.1418092909535, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082165, Length=417, Percent_Identity=38.1294964028777, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1788554, Length=419, Percent_Identity=37.2315035799523, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1788683, Length=427, Percent_Identity=28.8056206088993, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI25147385, Length=411, Percent_Identity=32.1167883211679, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI133906874, Length=396, Percent_Identity=31.0606060606061, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17535921, Length=414, Percent_Identity=30.6763285024155, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=447, Percent_Identity=27.9642058165548, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17535917, Length=146, Percent_Identity=33.5616438356164, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322031, Length=361, Percent_Identity=34.0720221606648, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6325229, Length=413, Percent_Identity=31.4769975786925, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24655093, Length=418, Percent_Identity=35.1674641148325, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI17648125, Length=413, Percent_Identity=34.8668280871671, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24640423, Length=417, Percent_Identity=31.6546762589928, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI17137578, Length=447, Percent_Identity=29.3064876957494, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42090; Mature: 41959

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAYIYDHVRTPRGRGKPDGSLHEVTALRLAETALRALKDRNGLDTRQVDDVVLGCVDP
CCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
VGEAGGDIARAAALVADYGTHVPGVQINRFCASGLDAINFAAAQVMAGQHDLTVGGGVES
HCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCHHH
MSRVGLGASGGAWPVDPAIAVKSYFMPQGVSADLIATKYGFSRDDCDAYAVESQKRSAQS
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHECCCCCCCCCCHHHHHHHHHHHHH
WGEGLFKNSVVPVRDINGITLLDRDEHMRPTTDMQSLASLKPSFVQMGQMGGFDAVAVDA
HHHHHHHCCCCCEECCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECC
HPDVEAVRHVHHAGNSSGIVDGAAAVLIGSREAGDAAGLRPRARIRAFANIGSDPALMLT
CCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
GPVDVTKKVLARAGMSLSDIDLFEINEAFASVVLRFHQAFDLDPAKVNVNGGAIAMGHPL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCC
GATGAMILGTVLDELERTGKERALVTLCIGAGMGTATIIERV
CCHHHHHHHHHHHHHHHCCCHHEEEEEEEECCCCHHHHHHCC
>Mature Secondary Structure 
PEAYIYDHVRTPRGRGKPDGSLHEVTALRLAETALRALKDRNGLDTRQVDDVVLGCVDP
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
VGEAGGDIARAAALVADYGTHVPGVQINRFCASGLDAINFAAAQVMAGQHDLTVGGGVES
HCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCHHH
MSRVGLGASGGAWPVDPAIAVKSYFMPQGVSADLIATKYGFSRDDCDAYAVESQKRSAQS
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHECCCCCCCCCCHHHHHHHHHHHHH
WGEGLFKNSVVPVRDINGITLLDRDEHMRPTTDMQSLASLKPSFVQMGQMGGFDAVAVDA
HHHHHHHCCCCCEECCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECC
HPDVEAVRHVHHAGNSSGIVDGAAAVLIGSREAGDAAGLRPRARIRAFANIGSDPALMLT
CCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
GPVDVTKKVLARAGMSLSDIDLFEINEAFASVVLRFHQAFDLDPAKVNVNGGAIAMGHPL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCC
GATGAMILGTVLDELERTGKERALVTLCIGAGMGTATIIERV
CCHHHHHHHHHHHHHHHCCCHHEEEEEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]