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The map label for this gene is splB [H]

Identifier: 218531389

GI number: 218531389

Start: 3643018

End: 3644082

Strand: Reverse

Name: splB [H]

Synonym: Mchl_3457

Alternate gene names: 218531389

Gene position: 3644082-3643018 (Counterclockwise)

Preceding gene: 218531390

Following gene: 218531388

Centisome position: 63.07

GC content: 66.38

Gene sequence:

>1065_bases
ATGGCCCCGCGCGACCTCATCGACATCGACCGCATCTTCCACGAGCCGGCGGTCGCCGATTATCCGCGCGGACGCGAGAT
CCTGGCCCGCTATCCGAACGCCGAGCGGATCGCGGTCCCGTCGCACTGGAACATCCCGGAGCTGCACGGCAATGCCGGCT
CGGTCGAGGACTGGGTGCGGATCAAGCGATCGACCCTGGTGCTCGGCGTGAAGAAGGGGCTGGCGATGCGGCCCAACGGC
CGTTCCGCCCACTTCATCGCGCCCTCGACCTCCAACGGTTGCGCGATGGCCTGCGCCTATTGCTACGTGCCGCGCCGCAA
GGGCTTTTCCAATCCGATCTCGCTGTTCGTGAATGTCGACGCGGTGTGCGCGGCGATCACCCGCCATGCGGCCAAGCAGG
GCCCGCTGCCGGAGCCCGATCAGATCGACCCGCAAGCCTGGGTCTACGATCTCGGCGAGAACGGCGACCTCTCGGTCGAT
GCGATGATCTGCGACAACGTCCGCGATCTCGTCGCGCAATTTCGCGGGATTCCCAACGCCAAGGGTTCATTCGCGACCAA
GGCCGTGAACCGGGATCTCCTCGCCTACGATCCGCAAGGCCGCACCCGCATCCGCTTCTCGCTGATGCCGGCGCGGATCG
CGCGGGTCGTCGATGTGCGCACCGCGCCGATCCCCGAGCGGATCGCCGCGATCGACGACTTCCTGCGGGCCGGCTACGAG
GTCCACGTCAATTTCTCCCCCGTGATCCTCTACGAGGGCTGGGAGGCGGATTGGGCCGCCCTGTTCGACGAGATCGACGG
GACGATCTCGAATGCCGCCAAGAATCAGCTCGCCTGCGAGATCATCATGCTGACCCACAATGCCGGGCTGCACGAGGTGA
ACCTCGGCTGGCACCCCAAGGCCGAGGATCTCCTCTGGCGGCCGGACATCCAGGAGACCAAGCGTTCGGAAGGCGGCGGC
GAAAACCTGCGCTACCGCACCGGCTGGAAAGGGAAATGGCTCGCCCGCTTCAAGGCGCTGCTCGCCGAGCGGATGCCCTA
TTGCCGGGTGCGCTACGCATTCTGA

Upstream 100 bases:

>100_bases
AGTTCTCCGCCAAGAAGGCGGAGCTGCTGGCGCGGCTCTGACAGGACGGCCTGTCGCCGCAGGCCCCGCCGGAAGGCCGC
GCTCGCCCTAGATCCAGGCC

Downstream 100 bases:

>100_bases
GGAGAAGAAAACCGATGCCGGACCACGAGAGCTATCTGCGCGAGGCGACCGAAATCGCCCTCGCCAACGTCGCCGAGGGT
GGCCGACCCTACGGCGCGGT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MAPRDLIDIDRIFHEPAVADYPRGREILARYPNAERIAVPSHWNIPELHGNAGSVEDWVRIKRSTLVLGVKKGLAMRPNG
RSAHFIAPSTSNGCAMACAYCYVPRRKGFSNPISLFVNVDAVCAAITRHAAKQGPLPEPDQIDPQAWVYDLGENGDLSVD
AMICDNVRDLVAQFRGIPNAKGSFATKAVNRDLLAYDPQGRTRIRFSLMPARIARVVDVRTAPIPERIAAIDDFLRAGYE
VHVNFSPVILYEGWEADWAALFDEIDGTISNAAKNQLACEIIMLTHNAGLHEVNLGWHPKAEDLLWRPDIQETKRSEGGG
ENLRYRTGWKGKWLARFKALLAERMPYCRVRYAF

Sequences:

>Translated_354_residues
MAPRDLIDIDRIFHEPAVADYPRGREILARYPNAERIAVPSHWNIPELHGNAGSVEDWVRIKRSTLVLGVKKGLAMRPNG
RSAHFIAPSTSNGCAMACAYCYVPRRKGFSNPISLFVNVDAVCAAITRHAAKQGPLPEPDQIDPQAWVYDLGENGDLSVD
AMICDNVRDLVAQFRGIPNAKGSFATKAVNRDLLAYDPQGRTRIRFSLMPARIARVVDVRTAPIPERIAAIDDFLRAGYE
VHVNFSPVILYEGWEADWAALFDEIDGTISNAAKNQLACEIIMLTHNAGLHEVNLGWHPKAEDLLWRPDIQETKRSEGGG
ENLRYRTGWKGKWLARFKALLAERMPYCRVRYAF
>Mature_353_residues
APRDLIDIDRIFHEPAVADYPRGREILARYPNAERIAVPSHWNIPELHGNAGSVEDWVRIKRSTLVLGVKKGLAMRPNGR
SAHFIAPSTSNGCAMACAYCYVPRRKGFSNPISLFVNVDAVCAAITRHAAKQGPLPEPDQIDPQAWVYDLGENGDLSVDA
MICDNVRDLVAQFRGIPNAKGSFATKAVNRDLLAYDPQGRTRIRFSLMPARIARVVDVRTAPIPERIAAIDDFLRAGYEV
HVNFSPVILYEGWEADWAALFDEIDGTISNAAKNQLACEIIMLTHNAGLHEVNLGWHPKAEDLLWRPDIQETKRSEGGGE
NLRYRTGWKGKWLARFKALLAERMPYCRVRYAF

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR004594 [H]

Pfam domain/function: NA

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 39672; Mature: 39541

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRDLIDIDRIFHEPAVADYPRGREILARYPNAERIAVPSHWNIPELHGNAGSVEDWVR
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHH
IKRSTLVLGVKKGLAMRPNGRSAHFIAPSTSNGCAMACAYCYVPRRKGFSNPISLFVNVD
HHHHEEEEEECCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHH
AVCAAITRHAAKQGPLPEPDQIDPQAWVYDLGENGDLSVDAMICDNVRDLVAQFRGIPNA
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCC
KGSFATKAVNRDLLAYDPQGRTRIRFSLMPARIARVVDVRTAPIPERIAAIDDFLRAGYE
CCCHHHHHHCCCCEEECCCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEE
VHVNFSPVILYEGWEADWAALFDEIDGTISNAAKNQLACEIIMLTHNAGLHEVNLGWHPK
EEEECCEEEEEECCCCHHHHHHHHHCCHHHHHHHCCEEEEEEEEECCCCCEEECCCCCCC
AEDLLWRPDIQETKRSEGGGENLRYRTGWKGKWLARFKALLAERMPYCRVRYAF
CCCCEECCCHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
APRDLIDIDRIFHEPAVADYPRGREILARYPNAERIAVPSHWNIPELHGNAGSVEDWVR
CCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHH
IKRSTLVLGVKKGLAMRPNGRSAHFIAPSTSNGCAMACAYCYVPRRKGFSNPISLFVNVD
HHHHEEEEEECCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHH
AVCAAITRHAAKQGPLPEPDQIDPQAWVYDLGENGDLSVDAMICDNVRDLVAQFRGIPNA
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCC
KGSFATKAVNRDLLAYDPQGRTRIRFSLMPARIARVVDVRTAPIPERIAAIDDFLRAGYE
CCCHHHHHHCCCCEEECCCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEE
VHVNFSPVILYEGWEADWAALFDEIDGTISNAAKNQLACEIIMLTHNAGLHEVNLGWHPK
EEEECCEEEEEECCCCHHHHHHHHHCCHHHHHHHCCEEEEEEEEECCCCCEEECCCCCCC
AEDLLWRPDIQETKRSEGGGENLRYRTGWKGKWLARFKALLAERMPYCRVRYAF
CCCCEECCCHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8449881; 9384377; 9353924 [H]