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The map label for this gene is yfcG [H]

Identifier: 218531385

GI number: 218531385

Start: 3640812

End: 3641519

Strand: Reverse

Name: yfcG [H]

Synonym: Mchl_3453

Alternate gene names: 218531385

Gene position: 3641519-3640812 (Counterclockwise)

Preceding gene: 218531388

Following gene: 218531382

Centisome position: 63.02

GC content: 66.81

Gene sequence:

>708_bases
ATGATCGACCTGCATTACTGGCCGACCCCGAACGGTCACAAGGTGACGATGTTTCTGGAGGAGGCCGGCCTCCCCTACAC
GATCCACCCGGTGGATATCGGCAAGGGCGCGCAGTTCGAGCCGGCCTTCCTGAAGATCGCCCCGAACAACCGGATGCCGG
CCATCGTCGATCACGAGCCGGCCGATAGCGGTGCGCCCGTCTCGCTGTTCGAGTCGGGCGCGATCCTGCTCTACCTCGCG
GAGAAGACCGGGCGCTTCCTGCCCGCCGACTTGCGCGGCCGGGCCGAGACCCTGCAATGGCTGTTCTGGCAGATGGGCGG
CCTCGGGCCGATGCTGGGCCAGAACCACCACTTCTCGCAATACGCGCCGGAGAAGATCCCCTACGCGATCGACCGCTACG
TCAAGGAGACGAACCGGCTCTACGGCGTGCTCGACCGGCGGCTTGCCGACCGCGCCTTCGTCGCGGGCGCCGATTACACC
ATCGCCGACATGGCTTCCTATCCCTGGATCGTGCCGTGGGAGAAGCAGGGCCAGCGCCTCGACGACCACCCCAACCTGAA
GCGCTGGTTCGAGGCGATCGCCGAGCGCGCCGCCACCAAAGCCGCCTATGCGCGGGCGGTGGAAGCCAACCCGAACCACG
GCCAGCCGATGAGCGAGGACGCGAAGAAGGTGATGTTCGGCCAGACCGCGACGAACACCAAGCGCTGA

Upstream 100 bases:

>100_bases
TCGAACGGGATCGGCCGCGGCTTGATCGGATCGCTCGACATTCCCACTTCGCCGCATGCCTGAGGCATCGCCCTCGTGGG
CGCGAAGACAAGGTGTCGCG

Downstream 100 bases:

>100_bases
GGCTTACCGCCCGCTGCGTCGGGCAGCCACCGTGGCGAGCGCGGCAAAATCCTTGTCGCCGTCGCCGTGGGCGATGGCGT
CGAGGAGGTTGTCGCGCAGC

Product: glutathione S-transferase domain

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIDLHYWPTPNGHKVTMFLEEAGLPYTIHPVDIGKGAQFEPAFLKIAPNNRMPAIVDHEPADSGAPVSLFESGAILLYLA
EKTGRFLPADLRGRAETLQWLFWQMGGLGPMLGQNHHFSQYAPEKIPYAIDRYVKETNRLYGVLDRRLADRAFVAGADYT
IADMASYPWIVPWEKQGQRLDDHPNLKRWFEAIAERAATKAAYARAVEANPNHGQPMSEDAKKVMFGQTATNTKR

Sequences:

>Translated_235_residues
MIDLHYWPTPNGHKVTMFLEEAGLPYTIHPVDIGKGAQFEPAFLKIAPNNRMPAIVDHEPADSGAPVSLFESGAILLYLA
EKTGRFLPADLRGRAETLQWLFWQMGGLGPMLGQNHHFSQYAPEKIPYAIDRYVKETNRLYGVLDRRLADRAFVAGADYT
IADMASYPWIVPWEKQGQRLDDHPNLKRWFEAIAERAATKAAYARAVEANPNHGQPMSEDAKKVMFGQTATNTKR
>Mature_235_residues
MIDLHYWPTPNGHKVTMFLEEAGLPYTIHPVDIGKGAQFEPAFLKIAPNNRMPAIVDHEPADSGAPVSLFESGAILLYLA
EKTGRFLPADLRGRAETLQWLFWQMGGLGPMLGQNHHFSQYAPEKIPYAIDRYVKETNRLYGVLDRRLADRAFVAGADYT
IADMASYPWIVPWEKQGQRLDDHPNLKRWFEAIAERAATKAAYARAVEANPNHGQPMSEDAKKVMFGQTATNTKR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=206, Percent_Identity=61.1650485436893, Blast_Score=265, Evalue=1e-72,
Organism=Escherichia coli, GI87082195, Length=213, Percent_Identity=45.0704225352113, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI87081789, Length=173, Percent_Identity=30.0578034682081, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324100, Length=232, Percent_Identity=33.1896551724138, Blast_Score=109, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864592, Length=196, Percent_Identity=28.0612244897959, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17864598, Length=206, Percent_Identity=29.126213592233, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17933730, Length=195, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646249, Length=195, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI85725204, Length=195, Percent_Identity=27.1794871794872, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17737923, Length=195, Percent_Identity=27.1794871794872, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26465; Mature: 26465

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLHYWPTPNGHKVTMFLEEAGLPYTIHPVDIGKGAQFEPAFLKIAPNNRMPAIVDHEP
CCCEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEECCCC
ADSGAPVSLFESGAILLYLAEKTGRFLPADLRGRAETLQWLFWQMGGLGPMLGQNHHFSQ
CCCCCCEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
YAPEKIPYAIDRYVKETNRLYGVLDRRLADRAFVAGADYTIADMASYPWIVPWEKQGQRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCEEECCHHCCCCC
DDHPNLKRWFEAIAERAATKAAYARAVEANPNHGQPMSEDAKKVMFGQTATNTKR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIDLHYWPTPNGHKVTMFLEEAGLPYTIHPVDIGKGAQFEPAFLKIAPNNRMPAIVDHEP
CCCEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEECCCC
ADSGAPVSLFESGAILLYLAEKTGRFLPADLRGRAETLQWLFWQMGGLGPMLGQNHHFSQ
CCCCCCEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
YAPEKIPYAIDRYVKETNRLYGVLDRRLADRAFVAGADYTIADMASYPWIVPWEKQGQRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCEEECCHHCCCCC
DDHPNLKRWFEAIAERAATKAAYARAVEANPNHGQPMSEDAKKVMFGQTATNTKR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]