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The map label for this gene is ispE

Identifier: 218531360

GI number: 218531360

Start: 3614031

End: 3614906

Strand: Reverse

Name: ispE

Synonym: Mchl_3428

Alternate gene names: 218531360

Gene position: 3614906-3614031 (Counterclockwise)

Preceding gene: 218531361

Following gene: 218531358

Centisome position: 62.56

GC content: 74.09

Gene sequence:

>876_bases
GTGTCCGTCCTCACCACCCGCGCTCCGGCCAAGATCAACCTGACCCTGCACATCCTCGGCCGCCGGCCCGGCGACGGCTA
CCATGCGCTGGAGAGCCTCGTGGCCTTCGCCGACGTGGCGGACACGCTGGAACTGGTGCCGGGCCCTGATCTGACGCTGG
ACATCTCCGGCCCCACCGCCGGGCCGGCTGGACCGCTCGACGACAACCTCGTCCTGCGCGCCGCCCGCCACCTCGCTGCG
GGCGTGGACGGCCTGAGGCTCGGCGCCTTTCGCCTGCACAAGCAACTGCCCGTGGCGGCGGGGATCGGCGGCGGCTCGTC
GGATGCGGCCGCTGCCCTGCGGCTGCTGGCCGAGCTGAATGGGCTCGCCCTTGATCACCCGGCGGTCATCGCGGCGGCGC
GGGCGACCGGCGCCGACGTGCCGGTCTGTCTCGATCCGCGGGCGCGGATGATGCGCGGCGCGGGCGAGGAAATCGGGCCG
GTGCTCGGCCTCGCATCGCTGCCGGCGGTGCTGGTCAATCCCGGCGTGCCGGTCTCGACCGCGCCGGTTTTCAAGGCGCT
CGGCCTCGCGGTCGGTCAGCGGCTCGACGGGGCGGAGCTTCCGGTCGTCGGGGCTAGCCTCAATGCGGACGCCGTGCTCG
CCGTCATCACCCCCGCTCGCAACGATCTTGAGGCGCCGGCCCTCACCGTCGCCCCGGTGATCGGCGAAGCGCTGGCGCTG
CTGCGGGCTCAGGCCGGGTGCCGGCTCGCGCGGATGTCGGGCTCCGGGGCCACCGTGTTCGCAATTTTTTCCGATGACGG
GGCGGCGGAGACGGCGGCCGCAGCGATCCGCACCGCCGAGCCGGGCTGGTGGGTCGAGCCGACGCGGCTCGCCTGA

Upstream 100 bases:

>100_bases
GGCCCGTCGGTCGCTGCGGGCAGGCGCCAACTGCCTTGCTCGTCGGGAAGGCTCCCCCTATGGCAGTGCCCGTCCCCCGC
CTCATCGGCCGAGAGCTCCT

Downstream 100 bases:

>100_bases
CGCGGCCGTGCCGCGGGCGCCTCAGTGCGCCCGGGCGATGCAGAAATCGACCACCTGCAGCAGGGCGGCCTTCATCGGCC
CCTGCGGGAACGGCTCAAGC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSVLTTRAPAKINLTLHILGRRPGDGYHALESLVAFADVADTLELVPGPDLTLDISGPTAGPAGPLDDNLVLRAARHLAA
GVDGLRLGAFRLHKQLPVAAGIGGGSSDAAAALRLLAELNGLALDHPAVIAAARATGADVPVCLDPRARMMRGAGEEIGP
VLGLASLPAVLVNPGVPVSTAPVFKALGLAVGQRLDGAELPVVGASLNADAVLAVITPARNDLEAPALTVAPVIGEALAL
LRAQAGCRLARMSGSGATVFAIFSDDGAAETAAAAIRTAEPGWWVEPTRLA

Sequences:

>Translated_291_residues
MSVLTTRAPAKINLTLHILGRRPGDGYHALESLVAFADVADTLELVPGPDLTLDISGPTAGPAGPLDDNLVLRAARHLAA
GVDGLRLGAFRLHKQLPVAAGIGGGSSDAAAALRLLAELNGLALDHPAVIAAARATGADVPVCLDPRARMMRGAGEEIGP
VLGLASLPAVLVNPGVPVSTAPVFKALGLAVGQRLDGAELPVVGASLNADAVLAVITPARNDLEAPALTVAPVIGEALAL
LRAQAGCRLARMSGSGATVFAIFSDDGAAETAAAAIRTAEPGWWVEPTRLA
>Mature_290_residues
SVLTTRAPAKINLTLHILGRRPGDGYHALESLVAFADVADTLELVPGPDLTLDISGPTAGPAGPLDDNLVLRAARHLAAG
VDGLRLGAFRLHKQLPVAAGIGGGSSDAAAALRLLAELNGLALDHPAVIAAARATGADVPVCLDPRARMMRGAGEEIGPV
LGLASLPAVLVNPGVPVSTAPVFKALGLAVGQRLDGAELPVVGASLNADAVLAVITPARNDLEAPALTVAPVIGEALALL
RAQAGCRLARMSGSGATVFAIFSDDGAAETAAAAIRTAEPGWWVEPTRLA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=282, Percent_Identity=31.5602836879433, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_METC4 (B7KUL9)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002422176.1
- ProteinModelPortal:   B7KUL9
- GeneID:   7116075
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_3428
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK14608
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29268; Mature: 29137

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLTTRAPAKINLTLHILGRRPGDGYHALESLVAFADVADTLELVPGPDLTLDISGPTA
CCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GPAGPLDDNLVLRAARHLAAGVDGLRLGAFRLHKQLPVAAGIGGGSSDAAAALRLLAELN
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHC
GLALDHPAVIAAARATGADVPVCLDPRARMMRGAGEEIGPVLGLASLPAVLVNPGVPVST
CCCCCCCHHEEEECCCCCCCCEEECCHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCH
APVFKALGLAVGQRLDGAELPVVGASLNADAVLAVITPARNDLEAPALTVAPVIGEALAL
HHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHHH
LRAQAGCRLARMSGSGATVFAIFSDDGAAETAAAAIRTAEPGWWVEPTRLA
HHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHEECCCCCCCCCCCCC
>Mature Secondary Structure 
SVLTTRAPAKINLTLHILGRRPGDGYHALESLVAFADVADTLELVPGPDLTLDISGPTA
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GPAGPLDDNLVLRAARHLAAGVDGLRLGAFRLHKQLPVAAGIGGGSSDAAAALRLLAELN
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHC
GLALDHPAVIAAARATGADVPVCLDPRARMMRGAGEEIGPVLGLASLPAVLVNPGVPVST
CCCCCCCHHEEEECCCCCCCCEEECCHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCH
APVFKALGLAVGQRLDGAELPVVGASLNADAVLAVITPARNDLEAPALTVAPVIGEALAL
HHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHHH
LRAQAGCRLARMSGSGATVFAIFSDDGAAETAAAAIRTAEPGWWVEPTRLA
HHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA