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The map label for this gene is yhjG [H]

Identifier: 218531242

GI number: 218531242

Start: 3472144

End: 3473604

Strand: Reverse

Name: yhjG [H]

Synonym: Mchl_3293

Alternate gene names: 218531242

Gene position: 3473604-3472144 (Counterclockwise)

Preceding gene: 218531246

Following gene: 218531241

Centisome position: 60.12

GC content: 73.44

Gene sequence:

>1461_bases
ATGGCAGACGTCCTGATCGTCGGCGCCGGGCCGGTCGGCCTGACGCTCGCCTGCGAACTGGCGCGCTACGGCGTCGGCGT
CCGGCTGATCGACCGCGCGCCACAGGCGACGCAGACCTCGAAGGCGCTCGTCGTCTGGTCGCGCACCCTCGAATTGATGG
ACCGGATGGGCTGCACCGAGGCCTTCCTCGCGGCGGGTCTGCGCGCCCGCGGCGCGACTCTGCGCGGTGGCGGCCGGGTG
CTCGGCGGCCCGGACTTCTCCGACATCGCCAGCCCCTACGCCTTCGGGCTGATGATTCCGCAGAGCGACACCGAGCGGCT
GCTGGCCGAGCACCTGCGCGGCTTCGGGGTCGCGGTCGAGCGCGCGGTCGAACTCACGCGCTTCTCACAGACGCCTGATG
GGGTGGAGGCCCAGCTCTCCCACGCCGACGGACGCGAGGAGCGGGTGGCGACAGCCTTCCTGATCGGCTGCGACGGCGCC
CACAGCGCCGTGCGGCACGGGCTCGGTCTGCCCTTCCGCGGCACGGCGCAGGGCGACGAGTGGCTGCTCGCTGACATCCG
CCTCGACGGCGCGGGGGGCCCGCCCAGCGACGCGATCGCGGTCTATTTCCACCGGGACGGTCCGTTCGTGATCTTCCCGA
TCCCCGGCGGCCGGGTGCGGGTGGTCGCCACGGTCGGACGCGCGGACGGGCGGCCGCGACCCGATCCGACGCTGGAAGAG
GTGCAGGCCCTGGTCGCCGCGCGGGCCGGTCCCGGCCTCCGCGTCTTCGATCCGGTCTGGCTCAGCCGCTTCCGCATCCA
TGAGCGGAAGGTCTCGGAGTACGGCCGGGGCCGCGTGTTCCTGGCGGGCGATGCCGCGCATATCCACAGCCCGGCCGGCG
GCCAGGGCATGAATACCGGCATGCAGGACGCGGTGAACCTGGCCTGGAAACTAGCCCTGGTGCGACGTGGCGCGGCCGCC
CCATCGCTCCTCGACAGCTACAGTTCGGAGCGCAACGCCGTCGGCGAGATGGTCCTGCGCAATGCCGGGTGGCTCACTGC
CATGGCGACGCTCACCCACCCCCTCGCCCAGGCCGTCCGCAACCGCGCCCTGCGTCTGTTCCTCGGCTTTCACACCGTGC
GGCGCCGGATGGCGGCGACGATGAGCGAGATCGCGATCGGCTATCCCGCCTCTCCGCTCTCGATCGGGCCCGGCGCCGGC
CGGCGCTGGCGGCCGGAGGATACGGACGGCCCGCCGCCGGGCGCCGGCGCCGACCCACGCTTCGTCCTCTACGCGGCCGA
TGCCGAGCGCGGGACGGCGCTCGCCGCCCGCTTTCCCGGTCTGGTTCAGCCCGCACCACGAGCGAACCCCGAGCCGGAGC
GCCTGCATCTGGTGCGCCCCGACGGGTATGTCGGCCTCGCAGCCGGCGCGGCCGACTGGGACGCCGCGGCGCACTACCTG
AAAGAACGTGGACCGGTCTGA

Upstream 100 bases:

>100_bases
ACACGCATCCCAGGGCCATCAGCACGGATGGGCGCCCGACGCAAATGGCGGATTGATCGCGGAAGCCGGCCGGAGCAGCA
TGACACAGGAGGCATCGCCT

Downstream 100 bases:

>100_bases
ACGCGGTGCAGCCGAGGCGCCTGTTCGCGCGTTGAGGTAGCGATTCGACGGAGAACGCCGCCCACAATGAAATGGTTTGC
AATCAGCCCGCGGGATTTTG

Product: monooxygenase FAD-binding

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MADVLIVGAGPVGLTLACELARYGVGVRLIDRAPQATQTSKALVVWSRTLELMDRMGCTEAFLAAGLRARGATLRGGGRV
LGGPDFSDIASPYAFGLMIPQSDTERLLAEHLRGFGVAVERAVELTRFSQTPDGVEAQLSHADGREERVATAFLIGCDGA
HSAVRHGLGLPFRGTAQGDEWLLADIRLDGAGGPPSDAIAVYFHRDGPFVIFPIPGGRVRVVATVGRADGRPRPDPTLEE
VQALVAARAGPGLRVFDPVWLSRFRIHERKVSEYGRGRVFLAGDAAHIHSPAGGQGMNTGMQDAVNLAWKLALVRRGAAA
PSLLDSYSSERNAVGEMVLRNAGWLTAMATLTHPLAQAVRNRALRLFLGFHTVRRRMAATMSEIAIGYPASPLSIGPGAG
RRWRPEDTDGPPPGAGADPRFVLYAADAERGTALAARFPGLVQPAPRANPEPERLHLVRPDGYVGLAAGAADWDAAAHYL
KERGPV

Sequences:

>Translated_486_residues
MADVLIVGAGPVGLTLACELARYGVGVRLIDRAPQATQTSKALVVWSRTLELMDRMGCTEAFLAAGLRARGATLRGGGRV
LGGPDFSDIASPYAFGLMIPQSDTERLLAEHLRGFGVAVERAVELTRFSQTPDGVEAQLSHADGREERVATAFLIGCDGA
HSAVRHGLGLPFRGTAQGDEWLLADIRLDGAGGPPSDAIAVYFHRDGPFVIFPIPGGRVRVVATVGRADGRPRPDPTLEE
VQALVAARAGPGLRVFDPVWLSRFRIHERKVSEYGRGRVFLAGDAAHIHSPAGGQGMNTGMQDAVNLAWKLALVRRGAAA
PSLLDSYSSERNAVGEMVLRNAGWLTAMATLTHPLAQAVRNRALRLFLGFHTVRRRMAATMSEIAIGYPASPLSIGPGAG
RRWRPEDTDGPPPGAGADPRFVLYAADAERGTALAARFPGLVQPAPRANPEPERLHLVRPDGYVGLAAGAADWDAAAHYL
KERGPV
>Mature_485_residues
ADVLIVGAGPVGLTLACELARYGVGVRLIDRAPQATQTSKALVVWSRTLELMDRMGCTEAFLAAGLRARGATLRGGGRVL
GGPDFSDIASPYAFGLMIPQSDTERLLAEHLRGFGVAVERAVELTRFSQTPDGVEAQLSHADGREERVATAFLIGCDGAH
SAVRHGLGLPFRGTAQGDEWLLADIRLDGAGGPPSDAIAVYFHRDGPFVIFPIPGGRVRVVATVGRADGRPRPDPTLEEV
QALVAARAGPGLRVFDPVWLSRFRIHERKVSEYGRGRVFLAGDAAHIHSPAGGQGMNTGMQDAVNLAWKLALVRRGAAAP
SLLDSYSSERNAVGEMVLRNAGWLTAMATLTHPLAQAVRNRALRLFLGFHTVRRRMAATMSEIAIGYPASPLSIGPGAGR
RWRPEDTDGPPPGAGADPRFVLYAADAERGTALAARFPGLVQPAPRANPEPERLHLVRPDGYVGLAAGAADWDAAAHYLK
ERGPV

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=339, Percent_Identity=31.5634218289086, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 51681; Mature: 51549

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVLIVGAGPVGLTLACELARYGVGVRLIDRAPQATQTSKALVVWSRTLELMDRMGCTE
CCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHCCCHH
AFLAAGLRARGATLRGGGRVLGGPDFSDIASPYAFGLMIPQSDTERLLAEHLRGFGVAVE
HHHHHCHHHCCCEECCCCEEECCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
RAVELTRFSQTPDGVEAQLSHADGREERVATAFLIGCDGAHSAVRHGLGLPFRGTAQGDE
HHHHHHHCCCCCCCCHHHHHCCCCCHHHEEEEEEEECCCHHHHHHHCCCCCCCCCCCCCC
WLLADIRLDGAGGPPSDAIAVYFHRDGPFVIFPIPGGRVRVVATVGRADGRPRPDPTLEE
EEEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEEEEECCCCCCCCCCCHHHH
VQALVAARAGPGLRVFDPVWLSRFRIHERKVSEYGRGRVFLAGDAAHIHSPAGGQGMNTG
HHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCCCCC
MQDAVNLAWKLALVRRGAAAPSLLDSYSSERNAVGEMVLRNAGWLTAMATLTHPLAQAVR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NRALRLFLGFHTVRRRMAATMSEIAIGYPASPLSIGPGAGRRWRPEDTDGPPPGAGADPR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
FVLYAADAERGTALAARFPGLVQPAPRANPEPERLHLVRPDGYVGLAAGAADWDAAAHYL
EEEEECCCCCCCEEHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHH
KERGPV
HHCCCC
>Mature Secondary Structure 
ADVLIVGAGPVGLTLACELARYGVGVRLIDRAPQATQTSKALVVWSRTLELMDRMGCTE
CCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHCCCHH
AFLAAGLRARGATLRGGGRVLGGPDFSDIASPYAFGLMIPQSDTERLLAEHLRGFGVAVE
HHHHHCHHHCCCEECCCCEEECCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
RAVELTRFSQTPDGVEAQLSHADGREERVATAFLIGCDGAHSAVRHGLGLPFRGTAQGDE
HHHHHHHCCCCCCCCHHHHHCCCCCHHHEEEEEEEECCCHHHHHHHCCCCCCCCCCCCCC
WLLADIRLDGAGGPPSDAIAVYFHRDGPFVIFPIPGGRVRVVATVGRADGRPRPDPTLEE
EEEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEEEEECCCCCCCCCCCHHHH
VQALVAARAGPGLRVFDPVWLSRFRIHERKVSEYGRGRVFLAGDAAHIHSPAGGQGMNTG
HHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCCCCC
MQDAVNLAWKLALVRRGAAAPSLLDSYSSERNAVGEMVLRNAGWLTAMATLTHPLAQAVR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NRALRLFLGFHTVRRRMAATMSEIAIGYPASPLSIGPGAGRRWRPEDTDGPPPGAGADPR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
FVLYAADAERGTALAARFPGLVQPAPRANPEPERLHLVRPDGYVGLAAGAADWDAAAHYL
EEEEECCCCCCCEEHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHH
KERGPV
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]