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The map label for this gene is dosC [H]

Identifier: 218531235

GI number: 218531235

Start: 3464042

End: 3465100

Strand: Reverse

Name: dosC [H]

Synonym: Mchl_3286

Alternate gene names: 218531235

Gene position: 3465100-3464042 (Counterclockwise)

Preceding gene: 218531236

Following gene: 218531232

Centisome position: 59.97

GC content: 71.1

Gene sequence:

>1059_bases
ATGCAGGGACAGATGCAGCAGGCGGATCCGGACCTCGACCGCGCTCTGGCTCCCGCGCACCGCGCCTGGGAGGCGCTGCG
GCAGTATGCGCTGCCGCCCTCCCCGCGGAACTACGAATTGCTGCACACCTTCTTCGCCGGCGCCAATCCGCCGCTGAGCG
AACGCCTCGCGGCCTGTCTGGCACCCGGTGGAACGCTGACCGAGGAGCAGGCGAGCGCGCTCTACGACGCCTGCATCGCC
GGGACCGCGACAGAGGCCTCCGTGCTCGATGGGGCGGGCCGGCTGGCGGAGGCGGCCGGTACCCTGGCCGAGCGCGTCGC
GGGCAGTCGGAAGGCGCTCGCCGGCTATGGTGATGCCCTGACCCATTGGTCCGGCCGCCTGAATGCACGGCCGACCGCCG
ATGAGCTGATGACGGCGCTCGTCGCCCTGATCGGTGAGACCGAGCAGGCGCAGACCCGCAATCACCTGCTCGAGGAGCAG
CTCTCGGTCTCCGCCGCCCACATCGCCAAGCTGCACAAGGATCTCGTCGAGACGCAGCGCGCGGCGGCGACCGATGCCCT
GACGGGGCTTGCCAACCGCAAGACCTTCGACGGGTTCCTGCGCGACTCGATCGCCCGCACCCGGAGCGATTCCGGCAGCA
CTTTCTCCCTGCTCATGCTCGACATCGACCACTTCAAGGGTCTCAACGACCGGCACGGCCACCGCTTCGGCGATCAGGTG
CTGCGCCTCGTCGGACGGCTGCTCAGCGCGAAGACGACGGATCGCGACCTCGCCGCGCGCTACGGCGGCGAGGAATTCGC
GATCGTGCTCGGCGAGGCCGGCATCGGCGCCGGCGTCGCGCTCGCCCAGGAGATCTGCGGCCGGCTGCGGGGTCAGCGGC
TGGTCAAGCGCGGCACCGGCGATGTCGTGGCACAGGTCACGATCTCGGCTGGCGTCGCGCAATACAGGCCGGGCGAAGCC
GCCCGCGATGTGATCGAGCGGGCCGACCGCGCGCTCTACGAGGCCAAGCGGACCGGCCGCGACCGTGTCTGCCCGACGCC
GCGAGCTGCAGTCGCGTGA

Upstream 100 bases:

>100_bases
CCGACCCCGTTGGGTCGGGCACCGCTCTATCCTCTTGTTTGCGGCGCTTTCTTTCGATGAACCGGAACCCGCTTCGTCGG
AAAGCGCTCGAGGGGCGGTG

Downstream 100 bases:

>100_bases
GGTCTCGCGGCGATCAGCGGAAATCGGTCGGCGCGAGGCCGTAGCGGGCGATCTTGTCGTAGAGGGTCTTGCGCGGAACG
CCGAGGGACTCGGCCGCCAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MQGQMQQADPDLDRALAPAHRAWEALRQYALPPSPRNYELLHTFFAGANPPLSERLAACLAPGGTLTEEQASALYDACIA
GTATEASVLDGAGRLAEAAGTLAERVAGSRKALAGYGDALTHWSGRLNARPTADELMTALVALIGETEQAQTRNHLLEEQ
LSVSAAHIAKLHKDLVETQRAAATDALTGLANRKTFDGFLRDSIARTRSDSGSTFSLLMLDIDHFKGLNDRHGHRFGDQV
LRLVGRLLSAKTTDRDLAARYGGEEFAIVLGEAGIGAGVALAQEICGRLRGQRLVKRGTGDVVAQVTISAGVAQYRPGEA
ARDVIERADRALYEAKRTGRDRVCPTPRAAVA

Sequences:

>Translated_352_residues
MQGQMQQADPDLDRALAPAHRAWEALRQYALPPSPRNYELLHTFFAGANPPLSERLAACLAPGGTLTEEQASALYDACIA
GTATEASVLDGAGRLAEAAGTLAERVAGSRKALAGYGDALTHWSGRLNARPTADELMTALVALIGETEQAQTRNHLLEEQ
LSVSAAHIAKLHKDLVETQRAAATDALTGLANRKTFDGFLRDSIARTRSDSGSTFSLLMLDIDHFKGLNDRHGHRFGDQV
LRLVGRLLSAKTTDRDLAARYGGEEFAIVLGEAGIGAGVALAQEICGRLRGQRLVKRGTGDVVAQVTISAGVAQYRPGEA
ARDVIERADRALYEAKRTGRDRVCPTPRAAVA
>Mature_352_residues
MQGQMQQADPDLDRALAPAHRAWEALRQYALPPSPRNYELLHTFFAGANPPLSERLAACLAPGGTLTEEQASALYDACIA
GTATEASVLDGAGRLAEAAGTLAERVAGSRKALAGYGDALTHWSGRLNARPTADELMTALVALIGETEQAQTRNHLLEEQ
LSVSAAHIAKLHKDLVETQRAAATDALTGLANRKTFDGFLRDSIARTRSDSGSTFSLLMLDIDHFKGLNDRHGHRFGDQV
LRLVGRLLSAKTTDRDLAARYGGEEFAIVLGEAGIGAGVALAQEICGRLRGQRLVKRGTGDVVAQVTISAGVAQYRPGEA
ARDVIERADRALYEAKRTGRDRVCPTPRAAVA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=186, Percent_Identity=34.4086021505376, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787262, Length=194, Percent_Identity=35.5670103092784, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=37.9746835443038, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081881, Length=210, Percent_Identity=33.8095238095238, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=33.5329341317365, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=31.638418079096, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=34.8571428571429, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=34.4444444444444, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787802, Length=186, Percent_Identity=29.0322580645161, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=194, Percent_Identity=29.8969072164948, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=35.4037267080745, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=177, Percent_Identity=26.5536723163842, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 37582; Mature: 37582

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGQMQQADPDLDRALAPAHRAWEALRQYALPPSPRNYELLHTFFAGANPPLSERLAACL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
APGGTLTEEQASALYDACIAGTATEASVLDGAGRLAEAAGTLAERVAGSRKALAGYGDAL
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHH
THWSGRLNARPTADELMTALVALIGETEQAQTRNHLLEEQLSVSAAHIAKLHKDLVETQR
HHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAATDALTGLANRKTFDGFLRDSIARTRSDSGSTFSLLMLDIDHFKGLNDRHGHRFGDQV
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHCCCCCCCCHHHHHHH
LRLVGRLLSAKTTDRDLAARYGGEEFAIVLGEAGIGAGVALAQEICGRLRGQRLVKRGTG
HHHHHHHHHCCCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DVVAQVTISAGVAQYRPGEAARDVIERADRALYEAKRTGRDRVCPTPRAAVA
CCEEEHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure
MQGQMQQADPDLDRALAPAHRAWEALRQYALPPSPRNYELLHTFFAGANPPLSERLAACL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
APGGTLTEEQASALYDACIAGTATEASVLDGAGRLAEAAGTLAERVAGSRKALAGYGDAL
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHH
THWSGRLNARPTADELMTALVALIGETEQAQTRNHLLEEQLSVSAAHIAKLHKDLVETQR
HHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAATDALTGLANRKTFDGFLRDSIARTRSDSGSTFSLLMLDIDHFKGLNDRHGHRFGDQV
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHCCCCCCCCHHHHHHH
LRLVGRLLSAKTTDRDLAARYGGEEFAIVLGEAGIGAGVALAQEICGRLRGQRLVKRGTG
HHHHHHHHHCCCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DVVAQVTISAGVAQYRPGEAARDVIERADRALYEAKRTGRDRVCPTPRAAVA
CCEEEHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]