Click here to switch to the map view.

The map label for this gene is dctA [H]

Identifier: 218531232

GI number: 218531232

Start: 3459147

End: 3460490

Strand: Reverse

Name: dctA [H]

Synonym: Mchl_3283

Alternate gene names: 218531232

Gene position: 3460490-3459147 (Counterclockwise)

Preceding gene: 218531235

Following gene: 218531231

Centisome position: 59.89

GC content: 65.62

Gene sequence:

>1344_bases
ATGGCCACCGTGCCGTCCCCTTTGACCGCGCCCCACGCGCCGCCCGCACCTAAGCCGCTCTATCGCACGCTGTACTTCCA
GGTGCTGGTCGCCGTCGCCATCGGCATTGCCCTCGGGCATTTCTACCCGAAGCTCGGCGCCGACATGAAGCCGCTCGGCG
ACGCCTTCATCAAGCTCGTCAAGATGATCATCGCGCCGGTGATCTTCCTCACCGTCGTCTCCGGCATCGCCGGCATGACC
AATCTTGAGAAGGTCGGCCGCGTCGGCGGCAAGGCGCTCATCTACTTCATCACCTTCTCGACGCTGGCCCTGATCGTCGG
CCTCGTCGTCGCCAACGTGCTCCAGCCCGGCCACGGCCTGCATATCGACCCGAACTCGCTCGATCCGAAGGCGGTCGCGA
CCTATGCCGGCAAGGCCAAGGAGCAGAACATCGCCGACTTCCTGATGAACATCATCCCGACGACGGCCGTCGGTGCGTTC
GCGGGCGGTGAGATCCTCCAGGTGCTGTTCTTCTCGGTGCTGTTCGGCTTCGGCCTCGCCTTCCTCGGCGAGCGCGGCAA
GCCGGTGCTCGACATCATCAAGGTGATGTCGGAGGCGATCTTCGGCGTCGTCAACATCATCATGAAGGTCGCCCCCATCG
GTGCCTTCGGCGCGATGGCCTTCACCATCGGCAAGTACGGCATCTCCTCGCTCGCCAACCTCGCCTACCTCGTCGGCGCC
TTCTACCTGACTTCGGCGATCTTCGTGCTCGGCGTGCTCGGCGCGGTCGCCCGCTACAACGGCTTCTCCATTCTCAAGCT
CATCCGCTACATCAAGGAAGAGCTGATGCTGGTGCTCGGCACCTCCTCCTCGGAGTCGGCCCTGCCCTCGCTCATCGACA
AGATGGAGAAGGCCGGCTGCTCGCGCCCCGTCGTCGGCCTCGTGGTCCCGACCGGCTACTCGTTCAACCTCGACGGCACC
AACATCTACATGACGATGGCGGCGCTCTTCATCGCCCAGGCCACCGACACCCCGATCACCTATGGCGAGCAGATCCTGCT
GCTGCTGGTGGCCATGCTCTCCTCGAAGGGCGCTGCGGGCGTGACCGGCTCGGGCTTCATCACCTTGGCCGCGACGCTCG
CCGTCGTCCCCTCCGTGCCGGTCGTCGGCATGGCACTGATCCTCGGCATCGACCGCTTCATGTCGGAGTGCCGCGCCCTC
ACCAACTTCATCGGCAACGCGGTCGCCTGCATCGTCGTCGCCCGCTGGGAAGGTGAGGTCGACGAGGCCAAGCTTCACGC
CGCGCTGGGTGGCAAGCCGGTCGCCGAGGCGACCCCTGCCCCGGTCCTTCAGCCGGCTGAGTGA

Upstream 100 bases:

>100_bases
GAGGCCAAAAGCAAGAGCGGACGGTACCGGAATCCTCCTCGGGAGGCGTTCGGCGAACCGATCCGACGGACCGCCCACCG
TCCGACAGGGAGAAACGCCC

Downstream 100 bases:

>100_bases
TCCGGTCAGGAGGGCGCACCGTCCTGCGTCCTTCGTCCGGTATGGGAGCCGCGGCAGCCGGAAAAGGGCTGCCGCGGTTT
CTCGCTTTCTGAGGCCTCTC

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MATVPSPLTAPHAPPAPKPLYRTLYFQVLVAVAIGIALGHFYPKLGADMKPLGDAFIKLVKMIIAPVIFLTVVSGIAGMT
NLEKVGRVGGKALIYFITFSTLALIVGLVVANVLQPGHGLHIDPNSLDPKAVATYAGKAKEQNIADFLMNIIPTTAVGAF
AGGEILQVLFFSVLFGFGLAFLGERGKPVLDIIKVMSEAIFGVVNIIMKVAPIGAFGAMAFTIGKYGISSLANLAYLVGA
FYLTSAIFVLGVLGAVARYNGFSILKLIRYIKEELMLVLGTSSSESALPSLIDKMEKAGCSRPVVGLVVPTGYSFNLDGT
NIYMTMAALFIAQATDTPITYGEQILLLLVAMLSSKGAAGVTGSGFITLAATLAVVPSVPVVGMALILGIDRFMSECRAL
TNFIGNAVACIVVARWEGEVDEAKLHAALGGKPVAEATPAPVLQPAE

Sequences:

>Translated_447_residues
MATVPSPLTAPHAPPAPKPLYRTLYFQVLVAVAIGIALGHFYPKLGADMKPLGDAFIKLVKMIIAPVIFLTVVSGIAGMT
NLEKVGRVGGKALIYFITFSTLALIVGLVVANVLQPGHGLHIDPNSLDPKAVATYAGKAKEQNIADFLMNIIPTTAVGAF
AGGEILQVLFFSVLFGFGLAFLGERGKPVLDIIKVMSEAIFGVVNIIMKVAPIGAFGAMAFTIGKYGISSLANLAYLVGA
FYLTSAIFVLGVLGAVARYNGFSILKLIRYIKEELMLVLGTSSSESALPSLIDKMEKAGCSRPVVGLVVPTGYSFNLDGT
NIYMTMAALFIAQATDTPITYGEQILLLLVAMLSSKGAAGVTGSGFITLAATLAVVPSVPVVGMALILGIDRFMSECRAL
TNFIGNAVACIVVARWEGEVDEAKLHAALGGKPVAEATPAPVLQPAE
>Mature_446_residues
ATVPSPLTAPHAPPAPKPLYRTLYFQVLVAVAIGIALGHFYPKLGADMKPLGDAFIKLVKMIIAPVIFLTVVSGIAGMTN
LEKVGRVGGKALIYFITFSTLALIVGLVVANVLQPGHGLHIDPNSLDPKAVATYAGKAKEQNIADFLMNIIPTTAVGAFA
GGEILQVLFFSVLFGFGLAFLGERGKPVLDIIKVMSEAIFGVVNIIMKVAPIGAFGAMAFTIGKYGISSLANLAYLVGAF
YLTSAIFVLGVLGAVARYNGFSILKLIRYIKEELMLVLGTSSSESALPSLIDKMEKAGCSRPVVGLVVPTGYSFNLDGTN
IYMTMAALFIAQATDTPITYGEQILLLLVAMLSSKGAAGVTGSGFITLAATLAVVPSVPVVGMALILGIDRFMSECRALT
NFIGNAVACIVVARWEGEVDEAKLHAALGGKPVAEATPAPVLQPAE

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=461, Percent_Identity=25.5965292841649, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI169790839, Length=420, Percent_Identity=25, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI194239697, Length=466, Percent_Identity=22.7467811158798, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI40254478, Length=429, Percent_Identity=24.4755244755245, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI21314632, Length=444, Percent_Identity=26.3513513513513, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI66773030, Length=397, Percent_Identity=23.9294710327456, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI5032093, Length=385, Percent_Identity=26.7532467532467, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI223468566, Length=288, Percent_Identity=27.4305555555556, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI223468564, Length=259, Percent_Identity=28.1853281853282, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI262359914, Length=352, Percent_Identity=24.1477272727273, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI301601644, Length=201, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=62.8019323671498, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1790514, Length=421, Percent_Identity=40.6175771971496, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI1788024, Length=401, Percent_Identity=22.6932668329177, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789473, Length=265, Percent_Identity=26.0377358490566, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=421, Percent_Identity=26.1282660332542, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71983099, Length=436, Percent_Identity=25.2293577981651, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983106, Length=436, Percent_Identity=25.2293577981651, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71996953, Length=460, Percent_Identity=25, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193206505, Length=447, Percent_Identity=24.3847874720358, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI193206654, Length=468, Percent_Identity=21.3675213675214, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=403, Percent_Identity=21.8362282878412, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24583025, Length=402, Percent_Identity=24.8756218905473, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137668, Length=402, Percent_Identity=24.8756218905473, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24583023, Length=402, Percent_Identity=24.8756218905473, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281360481, Length=481, Percent_Identity=22.6611226611227, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281360483, Length=481, Percent_Identity=22.6611226611227, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137666, Length=481, Percent_Identity=22.6611226611227, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46719; Mature: 46588

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATVPSPLTAPHAPPAPKPLYRTLYFQVLVAVAIGIALGHFYPKLGADMKPLGDAFIKLV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KMIIAPVIFLTVVSGIAGMTNLEKVGRVGGKALIYFITFSTLALIVGLVVANVLQPGHGL
HHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HIDPNSLDPKAVATYAGKAKEQNIADFLMNIIPTTAVGAFAGGEILQVLFFSVLFGFGLA
CCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FLGERGKPVLDIIKVMSEAIFGVVNIIMKVAPIGAFGAMAFTIGKYGISSLANLAYLVGA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FYLTSAIFVLGVLGAVARYNGFSILKLIRYIKEELMLVLGTSSSESALPSLIDKMEKAGC
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
SRPVVGLVVPTGYSFNLDGTNIYMTMAALFIAQATDTPITYGEQILLLLVAMLSSKGAAG
CCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
VTGSGFITLAATLAVVPSVPVVGMALILGIDRFMSECRALTNFIGNAVACIVVARWEGEV
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCH
DEAKLHAALGGKPVAEATPAPVLQPAE
HHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA