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The map label for this gene is fusA [H]

Identifier: 218531230

GI number: 218531230

Start: 3457446

End: 3458330

Strand: Reverse

Name: fusA [H]

Synonym: Mchl_3281

Alternate gene names: 218531230

Gene position: 3458330-3457446 (Counterclockwise)

Preceding gene: 218531231

Following gene: 218531226

Centisome position: 59.85

GC content: 63.95

Gene sequence:

>885_bases
ATGCCGAGTGCGCCGTGGCCGCTGATCGAGATCTCCGTCGCGGCGGTGTCCGACGATAACCGGAGCAACCTGCGCAGCGC
CTTGCTGGAACTGGTGGCCGAGACTCCGGGCCTCGATTTCTCGACCGATTCGACGACCGGCCAATTCATCTTCAAAGCCG
GGACCGAGAGTTCTCTCGGCGCCGCACTCGAGCGTCTCCGAGCCGGTCCGATCGCCTTCGAGACCGGGCAGCTGCAGGTC
GCTTATCGCGAGAGACTGACCCGGCGACGTGAGATCGACTTCCTGCACAAGCGTGTGATCAAAGGCGACAAAGGCCAGTT
CGCGCGCGTGAAGCTCCGGTTCGAACCGAGCGAGCCCGGTGTCGGCAACGTGTTCAACGCGAAGATCCCCGAAGCGATTC
TGCCGAGGGGGTACGTCGATGGTATCGAGAGGGGCGTCGCTTCGGTGCTGGCCGCCGGTGTCGTGGCCGGCTGTCCCGTT
ATCGAGGTCACCGCTGAACTGATCGACGGCGCTTATCACGAAATCGATTCTTCGCCCCTCGCCTTCGAAATTGCAGCCCG
CGCGGCTTTCCGTGAGGCGCTTCACGGCAGCTCCGTGCTCGCCGAGCCGATCATGGCAGTCGAAATCGCGGTACCGGAGC
GGTCCGCCGCACGGGTGATCAGCGACTTGCAAGGTCGCCGCGGCCTGATCCAGGACAGGAGCGTGCGCAGCGACGCGACC
ATCATCACCGCGACGGTGCCGCTTGCTGAAATGCTTGGTTTTGGTTCGCGCCTGCAATCCGTGGCCGGCGACGAAGCCTG
CTTTTCAATGGCATTCAGCCATTATGCACCCGTCCCGTCCTTGGATCTCGATCCGCCGCCGGTGATTGCTGCGCTACGCG
CGTGA

Upstream 100 bases:

>100_bases
ATGCGTGGGGCTTTGTATTCTACTCTATGTTGCATTTACCTGATGCGCCTGCGAAGCAGAACGGGCGGATAGCCGGTCCG
TCGCCTGCCAAGAGCCCGTC

Downstream 100 bases:

>100_bases
TGATCTTGCGCCGAGCGTGAGAGCGCTCGCGCAAGAACATCAGAACGCCGACAGCCGTCGACGAACCCGCTCGATGAACC
GTGCGCGCATCGGCTGCGTG

Product: elongation factor G domain IV

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MPSAPWPLIEISVAAVSDDNRSNLRSALLELVAETPGLDFSTDSTTGQFIFKAGTESSLGAALERLRAGPIAFETGQLQV
AYRERLTRRREIDFLHKRVIKGDKGQFARVKLRFEPSEPGVGNVFNAKIPEAILPRGYVDGIERGVASVLAAGVVAGCPV
IEVTAELIDGAYHEIDSSPLAFEIAARAAFREALHGSSVLAEPIMAVEIAVPERSAARVISDLQGRRGLIQDRSVRSDAT
IITATVPLAEMLGFGSRLQSVAGDEACFSMAFSHYAPVPSLDLDPPPVIAALRA

Sequences:

>Translated_294_residues
MPSAPWPLIEISVAAVSDDNRSNLRSALLELVAETPGLDFSTDSTTGQFIFKAGTESSLGAALERLRAGPIAFETGQLQV
AYRERLTRRREIDFLHKRVIKGDKGQFARVKLRFEPSEPGVGNVFNAKIPEAILPRGYVDGIERGVASVLAAGVVAGCPV
IEVTAELIDGAYHEIDSSPLAFEIAARAAFREALHGSSVLAEPIMAVEIAVPERSAARVISDLQGRRGLIQDRSVRSDAT
IITATVPLAEMLGFGSRLQSVAGDEACFSMAFSHYAPVPSLDLDPPPVIAALRA
>Mature_293_residues
PSAPWPLIEISVAAVSDDNRSNLRSALLELVAETPGLDFSTDSTTGQFIFKAGTESSLGAALERLRAGPIAFETGQLQVA
YRERLTRRREIDFLHKRVIKGDKGQFARVKLRFEPSEPGVGNVFNAKIPEAILPRGYVDGIERGVASVLAAGVVAGCPVI
EVTAELIDGAYHEIDSSPLAFEIAARAAFREALHGSSVLAEPIMAVEIAVPERSAARVISDLQGRRGLIQDRSVRSDATI
ITATVPLAEMLGFGSRLQSVAGDEACFSMAFSHYAPVPSLDLDPPPVIAALRA

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=285, Percent_Identity=27.3684210526316, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI19923640, Length=279, Percent_Identity=27.5985663082437, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI25306287, Length=279, Percent_Identity=27.5985663082437, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1789738, Length=282, Percent_Identity=34.3971631205674, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17533571, Length=283, Percent_Identity=25.7950530035336, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=273, Percent_Identity=25.6410256410256, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=277, Percent_Identity=31.7689530685921, Blast_Score=125, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24582462, Length=281, Percent_Identity=29.8932384341637, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI221458488, Length=296, Percent_Identity=25.3378378378378, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 31402; Mature: 31271

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSAPWPLIEISVAAVSDDNRSNLRSALLELVAETPGLDFSTDSTTGQFIFKAGTESSLG
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHH
AALERLRAGPIAFETGQLQVAYRERLTRRREIDFLHKRVIKGDKGQFARVKLRFEPSEPG
HHHHHHHCCCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
VGNVFNAKIPEAILPRGYVDGIERGVASVLAAGVVAGCPVIEVTAELIDGAYHEIDSSPL
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCH
AFEIAARAAFREALHGSSVLAEPIMAVEIAVPERSAARVISDLQGRRGLIQDRSVRSDAT
HHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCE
IITATVPLAEMLGFGSRLQSVAGDEACFSMAFSHYAPVPSLDLDPPPVIAALRA
EEEECCCHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
PSAPWPLIEISVAAVSDDNRSNLRSALLELVAETPGLDFSTDSTTGQFIFKAGTESSLG
CCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHH
AALERLRAGPIAFETGQLQVAYRERLTRRREIDFLHKRVIKGDKGQFARVKLRFEPSEPG
HHHHHHHCCCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
VGNVFNAKIPEAILPRGYVDGIERGVASVLAAGVVAGCPVIEVTAELIDGAYHEIDSSPL
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCH
AFEIAARAAFREALHGSSVLAEPIMAVEIAVPERSAARVISDLQGRRGLIQDRSVRSDAT
HHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCE
IITATVPLAEMLGFGSRLQSVAGDEACFSMAFSHYAPVPSLDLDPPPVIAALRA
EEEECCCHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA